人淋巴细胞转移因子对银屑病患者自然杀伤细胞活性的影响

本文用ＬＤＨ释放法测定了应用转移因子前后的银屑病患者外周血ＮＫ细胞活性，结果显示转移因子能显著地提高其ＮＫ细胞的杀伤活性，揭示了转移因子对银屑病患者细胞免疫功能的调节作用，佐证了其临床应用价值。同时讨论了转移因子对银屑病患者ＮＫ细胞的作用机制。     

银屑病患者外周血T细胞及其亚群和胸腺肽治疗的研究

应用抗人Ｔ细胞单克隆抗体碱性磷酸酶－抗碱性磷酸酶法对９２例寻常性银屑病患者外周血Ｔ细胞及其亚群进行检测，并用胸腺肽治疗其中３２例，观察疗效及治疗前后外周血Ｔ细胞及其亚群的变化，并与２６名正常人进行比较。结果显示，银屑病患者与正常人和治疗前后比较有显著不同。由此进一步支持银屑病患者存在细胞免疫功能异常。胸腺肽注射治疗收到较好效果。     

麻风患者NK细胞活性检测

大量实验资料证实 ,麻风患者体液免疫功能正常或部分过度 ,但细胞免疫出现不同程度的缺陷 ,原因还不太清楚 ,我们对麻风患者的免疫功能曾作过较全面的检测 ,并进行了系列报告。其结果与国内外大部分报道基本相同 ,迄今为止 ,还很少见到有关麻风患者ＮＫ细胞活性的检测报告。因此 ,我们进行了一定数量的麻风患者ＮＫ细胞活性的检测。结果报告如下。材料与方法　检测对象 :我们选择了恩施州二市三县皮防院麻风病区麻风患者6 0例 (ＬＬ型 2 0例 ,ＴＴ型 4 0例 ,病程 5～ 6 0年 ,男 4 3例 ,女 17例 ,年龄 18～ 75岁 ) ,另选当地 30名健康成年人 …     

银屑病患者骨髓基质细胞生物学活性研究

目的:通过比较银屑病患者与对照组骨髓基质细胞生物学活性(包括细胞形态、免疫表型、增殖活性、自发凋亡率以及分泌细胞因子)的差异,揭示银屑病患者骨髓基质细胞的异常.方法:采用密度梯度离心法分别分离患者组与对照组骨髓单一核细胞,通过贴壁法培养骨髓基质细胞,收集传3代后的骨髓基质细胞及细胞培养上清液,流式细胞术鉴定其免疫表型及细胞凋亡率、四甲基偶氮唑蓝(MTT)比色法检测其增殖活性以及ELISA法检测细胞培养上清液中白介素(IL)-6、血小板源性生长因子(PDGF)和表皮生长因子(EGF)的水平.结果:患者组和对照组比较,骨髓基质细胞形态相似、表型相同,但细胞增殖活性、凋亡率以及细胞因子分泌水平均有差异(P＜0.05).结论:银屑病患者骨髓基质细胞生物学活性异常,表明患者骨髓造血微环境可能存在异常.     

武警某部新兵军事训练前后NK细胞活性和T细胞转化功能测定

目的 研究武警某部新兵军事训练前后NK细胞活性和T细胞转化功能的变化.方法 采用乳酸脱氢酶（LDH）释放实验,测定NK细胞杀伤活性.采用MTT比色法,测定T淋巴细胞的增殖反应.结果 军事训练第二个月（应激期）,新兵NK细胞杀伤活性和T细胞转化功能与训练前期（基础期）和训练期第三个月后（恢复期）相比较差异有统计学意义（P〈0.05）,军事训练应激期,新兵NK细胞杀伤活性和T细胞转化功能明显升高.结论 军事训练可改变新兵NK细胞活性和T细胞转化功能状态,从而调节机体免疫平衡.     

银屑病患者的自然杀伤细胞活性测定

本文用乳酸脱氢酶释放法测定了３０例银屑病患者和２８例正常人的ＮＫ细胞活性，结果发现患者的ＮＫ细胞活性明显低于正常人，而且进展期患者的ＮＫ细胞活性也明显低于稳定期患者，提示ＮＫ细胞的活性与银屑病的发生和发展有关。     

A study on the candida-killing activity of polymorphonuclear leukocytes in patients with psoriasis vulgaris

Summary The Candida albicans killing capacity, a function of polymorphonuclear leukocytes from patients with psoriasis vulgaris was significantly higher than that of granulocytes from healthy persons. This observation is a further data supporting the concept of the activated granulocyte in psoriasis.     

Natural killer (NK) cell activity and NK-related cell surface markers in patients with atopic dermatitis

Natural killer (NK) cell activity and NK-related surface markers in peripheral blood mononuclear cells (PBMC) in thirty-three patients with adult type atopic dermatitis (AD) were investigated. NK cell activity, detectable by cytolysis of erythroleukemic cell line K562 cells, was reduced in patient PBMC as compared with healthy controls. Imbalances in the lymphocyte populations reactable with the monoclonal antibodies, anti-HNK-1, and anti-CD16 were found in the patient PBMC. Some positive correlations between these cell populations and the NK cell activities were recognized. In most cases, high levels of serum IgE were inversely correlated with NK cell activity. Depletion of the macrophages from patient PBMC had no effect on NK cell activities. These activities were enhanced by culture with β-interferon for 18 hours, as were the NK cell activities of healthy controls. These results suggest that reduced numbers or imbalances in the lymphocyte population with NK cell activity, are responsible for the pathogenesis and the prognosis of adult type atopic dermatitis.     

累及皮肤的结外鼻型NK/T细胞淋巴瘤

报告1例累及皮肤的结外鼻型NK/T细胞淋巴瘤.患者男,72岁.确诊结外鼻型NK/T细胞淋巴瘤8年后,鼻根部出现红肿、破溃.根据皮损、组织病理检查和免疫组化染色结果确诊.     

Red blood cell distribution width is increased in patients with psoriasis vulgaris: A retrospective study on 261 patients

Circulating inflammatory cytokines and markers are increased in patients with psoriasis. Recent studies have shown that a higher red blood cell distribution width (RDW) is associated with disease activity in various disorders. Our objective was to investigate whether RDW is increased in psoriasis patients, and to evaluate its possible association with disease severity. We conducted a retrospective study of psoriasis patients seen in a university hospital in South Korea. Information about demographics, hematological parameters and disease severity were collected. Statistical analysis was performed using Student's t‐test, multivariable logistic regression, Fisher's exact test and Spearman's rank correlation coefficient analysis as appropriate. A total of 261 psoriasis patients and 102 healthy controls were included in our study. The mean RDW value was significantly increased in psoriasis patients compared with healthy control (P = 0.037). Compared with mild psoriasis patients (Psoriasis Area and Severity Index [PASI], <7), moderate to severe patients (PASI, ≥7) showed significantly higher RDW values (P = 0.044). However, RDW did not show significant correlation with PASI (P = 0.358). When patients were divided into two groups according to their RDW value (<14.6% and ≥14.6%), the mean value of PASI was not significantly different (P = 0.219). Patients with psoriasis showed increased RDW values compared with healthy controls. It was also higher in the moderate and severe disease group than the mild group. Though this is only a pilot study, it is possible that RDW value can reflect the inflammatory status of psoriasis patients.     

他克莫司软膏联合激光治疗对寻常型白癜风疗效及细胞免疫的影响

目的观察他克莫司软膏联合激光治疗寻常型白癜风的效果及对T细胞亚群、趋化因子9(C-X-C motif9,CXCL9)表达的影响。方法选取我院2014年1月至2017年1月期间收治的150例寻常型白癜风患者,依据随机数字表法分为对照组1和对照组2,以及观察组,每组各50例,其中对照组1采用他克莫司软膏治疗,对照组2采用激光治疗,观察组联合采用他克莫司软膏、激光治疗。比较三组患者临床疗效,以及血清CXCL9水平、T细胞亚群。结果观察组总有效率(89.8%)显著高于对照组1(43.8%)和对照组2(56.3%),差异均具有统计学意义(P <0.05)。观察组治疗后的血清CXCL9水平、T总、CD8+T显著低于对照组1和对照组2,CD4+T/CD8+T显著高于对照组1和对照组2(P <0.05)。三组患者不良反应发生率不存在显著差异(P <0.05)。结论他克莫司软膏联合激光治疗可有效降低寻常型白癜风患者CXCL9表达,改善T细胞亚群比例,提高治疗效果。     

Topical treatment of psoriasis with the topoisomerase inhibitors novobiocin and nalidixic acid: A pilot study

Summary Our studies in human epidermal keratinocytes as a model system have suggested that the antibiotic topoisomerase II inhibitors, novobiocin and nalidixic acid, may be of value for the treatment of hyperproliferative skin disorders. We have therefore conducted a pilot study of the clinical efficacy of these compounds for the treatment of psoriasis. The compounds were administered topically to psoriatic plaques in seven healthy patients over a period of 6 weeks. Nalidixic acid (2%) or novobiocin (2% or 5%) in methylcellulose were applied twice daily under occlusion, and methylcellulose alone was used as a control. In six of the seven patients, one or both compounds effected somewhat greater improvement than in the control within 3 weeks of treatment.     

Establishment and characterization of a novel human cell line exhibiting both immunophenotypic markers of monocyte/macrophage and natural killer cell lineages from peripheral blood of a patient with atopic dermatitis

Abstract Large amounts of homogeneous cells are not always available for in vitro studies of inflammatory skin disorders. Here we demonstrate that a novel cell line, termed YAA, has been established from peripheral blood mononuclear cells, which were separated by the Ficoll method, of a patient with atopic dermatitis. YAA cells were grown in suspension culture. The cytochemical staining showed a positive reaction for α-naphthyl butyrate esterase, which was completely inhibited by sodium fluoride, but a negative result for periodic acid-Schiff, peroxidase and alkaline phosphatase. A large population of YAA cells exhibited phenotype of CD33 and CD56 but neither of CD2 nor CD3. The phorbol ester-stimulated YAA cells produced a considerable amount of tumor neerosis factor-α. These findings suggest that YAA might be a monocytoid line with an additional phcnolype specific for natural killer cells.     

NB-UVB联合阿维A治疗寻常型银屑病及其对红细胞天然免疫功能的影响

目的观察窄谱中波紫外线(NB-UVB)联合阿维A治疗寻常型银屑病的疗效及安全性,了解治疗前后患者红细胞天然免疫功能的变化。方法对30例中重度寻常型银屑病患者采用NB-UVB3次/W照射配合阿维A10mg2次/d,疗程8周,用PASI积分评价疗效,同时应用流式细胞仪检测治疗前后患者血浆红细胞CD35(CR1,补体Ⅰ型受体)的表达变化。结果 30例银屑病患者治愈率40%;治疗前红细胞CD35分子定量(70.04±27.05),治疗后CD35分子定量(59.00±17.24),治疗前后差异有统计学意义(t=2.097,P<0.05),且治疗前后红细胞CD35定量与PASI评分呈明显正相关(γ=0.924,P<0.001)。结论 NB-UVB联合阿维A治疗寻常型银屑病有疗效确切,同时测定患者红细胞天然免疫功能可作辅助性观察和评价治疗效果。