Antimicrobial proteins of maternal and cord sera and human milk in relation to maternal nutritional status

Antimicrobial proteins in maternal and cord sera and sequential samples of human milk were studied in a group of 60 Chinese women to determine the degrees of passive immunity transferred from women of different nutritional status to their infants. Maternal malnutrition was characterized by low values for wt/ht2 and serum total protein and albumin. Maternal immunoglobulin (IgG) concentrations were not significantly different between well- and malnourished groups prepartum but were significantly different postpartum. Mean concentrations of cord IgG and lysozyme from well- and malnourished groups were not statistically different. During the first 7 d of lactation and most stages thereafter, mean concentrations of IgA; complements C3 and C4, and lysozyme in milk from the malnourished group were only half of those of the well-nourished group. Antimicrobial proteins transferred via milk to newborns may be influenced by the mother's nutritional status.     

Iron, zinc, folate and vitamin B12 nutritional status and milk composition of low-income Brazilian mothers

The purpose of this study was to assess the nutritional status of low-income Brazilian mothers, who were supplemented with iron and vitamin B12 during pregnancy, in terms of iron, zinc, folate and vitamin B12, in different stages of lactation and to determine the influence of the maternal nutritional status on milk composition. The effect of folate supplementation during pregnancy on folate status of the nursing mothers and milk composition was investigated. The effect of partial weaning on maternal status and milk composition was also studied. In general, the nutritional status of iron, zinc, folate and vitamin B12 of the mothers appears adequate. However, some of the mothers had indices of status lower than normal limits for non-pregnant women. These values, particularly after 30 d post-partum, indicate that these mothers might be at nutritional risk and that the nutrient supplementation received during pregnancy was insufficient to meet demands. There was an increase with the stage of lactation for haematocrit, serum vitamin B12, serum zinc, serum albumin, milk folate and saturation of its binding protein, but there was a decrease for milk protein, total and whey-bound iron and zinc, and lactoferrin. Mothers who took folate supplements during pregnancy had higher serum folate levels immediately after birth than those not taking the supplements but no differences were found at later stages of lactation. Milk composition was not affected. Partial weaning did not affect the maternal nutritional status or the milk composition except for iron which was higher in milk from mothers who were partially breastfeeding.(ABSTRACT TRUNCATED AT 250 WORDS)     

Humoral defence factors in the breast milk of Ethiopian women with leprosy and healthy controls

Secretory IgA, lactoferrin, albumin, and total protein were quantitated in colostrum and milk samples obtained from 215 Ethiopian nursing mothers over a period ranging from 1 day to 2 yr postparturition. IgG, IgM, C3, and C4 complement components were quantitated in 11 day 1 samples. The subjects were classified into three groups: lepromatous leprosy, borderline lepromatous leprosy, and a nonlepromatous group consisting of women with tuberculoid leprosy and healthy controls. Results obtained from the above groups were also compared with a group from Edinburgh. No major variation in levels of secretory IgA, lactoferrin, albumin, and total protein was found between the three groups of Ethiopian women. Results from the Edinburgh group were significantly higher, mainly in the level of total protein. When the individual proteins were expressed as a percentage of the total protein, there was no difference between the milk samples from the Ethiopian and Edinburgh mothers.     

Premature delivery influences the immunological composition of colostrum and transitional and mature human milk

Human breast milk is the ideal nutrition for the newborn, and in addition to its nutritional contribution, necessary for infant growth and development, it contains various immune bioactive factors that confer some of the numerous beneficial effects of breastfeeding. The current study analyzed the concentrations of IgA, growth factors such as epidermal growth factor (EGF), TGFβ1, and TGFβ2, cytokines IL-6, IL-8, IL-10, IL-13, and TNFα, and TNF-receptor I (TNF-RI) in colostrum and transitional and mature milk from mothers with mature, premature, and very premature infants. Human milk samples were collected from mothers delivering at term (T), preterm (PT), and very preterm (VPT). Milk from all the mothers was collected at 3 different time points after delivery corresponding to colostrum and transitional and mature milk. After obtaining milk whey, IgA, EGF, TGFβ1, and TGFβ2 were determined by ELISA and IL-6, IL-8, IL-10, IL-13, TNFα and TNF-RI by cytometric bead array immunoassay. The colostrum of the PT group was extremely rich in most of the factors studied, but higher concentrations than in the T group were only found for IL-6 (P = 0.051), TGFβ1, and TGFβ2 (P < 0.05). Conversely, the colostrum of the VPT group had lower concentrations of IgA, IL-8, IL-10, and TNFα than those in the T group (P < 0.05). Results suggest that maternal lactogenic compensatory mechanisms accelerating the development of immature breast-fed preterm infants may take effect only after wk 30 of gestation.     

Discriminant function of complement C3, serum albumin and anthropometric measurements in identifying malnutrition

A prospective study was conducted on 32 malnourished and 12 healthy pre-school children to study the role of certain variables, viz., supine length, weight-for-supine length, complement C3, weight-for-head circumference, serum albumin, weight and mid-arm circumference, in detecting the presence of malnutrition and to grade the severity of malnutrition. Out of 32 malnourished children 14 cases were re-investigated after 15 days of nutritional rehabilitation to find out the significant discriminator(s) of pre and post-treatment condition. Step-down discriminant analysis showed that supine length, weight-for-supine length and complement C3 were efficient discriminators in detecting cases of malnutrition. To transform the variables, viz., supine length, weight-for-supine length and complement C3 (each expressed as a percentage of standard-for-age) into score form, respective simplified weights, for multiplication, were found to be 0.9, 0.4 and 0.1. A dividing score (Z) of 123.1, with a bordering range () of 119.6 to 126.7 for 90% probability limits, separated malnourished from healthy children. For classifying children with respect to severity of malnutrition, weight-for-supine length and serum albumin concentration were found to be significant discriminators. Malnourished children with oedema (N=11) had higher weight-for-supine length ratio and lower serum albumin concentration as compared to non-oedematous malnourished children (N=21). While oedematous and non-oedematous malnourished children were almost equally stunted, higher weight for supine length ratio was attributed to accumulation of oedema fluid, in the former group. A subsample of 14 malnourished children who had received a 15-day nutritional therapy could be differentiated from their earlier condition (before treatment) by a significant rise in complement C3 and serum albumin levels.     

母乳中甲状腺激素含量测定及其意义的初步探讨

本文动态观察了产后6周内母乳中T_3、T_4含量的变化,结果表明:母初乳、过渡乳和成熟乳中T_3、T_4含量无显著差异,初乳中T_3含量约为母血清T_3含量的1/3,但两者之间无显著相关关系,早产儿和足月儿的母初乳中T_3含量亦无显著差异。说明母乳中含有一定量的T_3,母乳中T_3不是从母血简单扩散而来,可能是T_4在乳腺中脱碘产生。     

Impact on colostrum retinol levels of immediate postpartum supplementation with retinyl palmitate]

OBJECTIVE: To assess the effect of retinyl palmitate supplementation on colostrum retinol levels, investigating the influence of maternal variables (age, type of delivery, and biochemical nutritional status) on these levels. METHOD: The study included 33 mothers receiving care at the Januário Cicco Maternity School, in the city of Natal, Rio Grande do Norte, Brazil. In the first hours after delivery, blood and colostrum samples were collected. Another colostrum sample was collected six hours after maternal supplementation with 200,000 IU of retinyl palmitate. Serum and colostrum retinol levels were determined by high-performance liquid chromatography. RESULTS: The mean retinol level in colostrum before the supplementation was 110.8 +/- 82.3 microg/dL, and after supplementation it was 164.4 +/- 106.5 microg/dL (P < 0.025). Of the 33 mothers, 12 of them either did not respond to supplementation or had an increase of less than 10% in colostrum retinol levels; serum retinol in these women was significantly lower as compared to the responders (P = 0.024). In comparison to women with a normal delivery, the mothers who underwent cesarean delivery (64%) had lower serum retinol levels, but not lower colostrum retinol levels (P = 0.036). Maternal age did not influence retinol levels in either serum or colostrum. CONCLUSION: The increase in colostrum retinol levels following vitamin A supplementation was sufficient to guarantee double the retinol requirements of a newborn infant.     

Effects of malnutrition during the reproductive cycle on nutritional status and lactational performance of rat dams

To assess the effects of malnutrition imposed at various stages of the reproductive cycle on maternal nutritional status and lactational performance, rats were fed 50% of the intake of control animals fed adlibitum (C) either before pregnancy (R-B), before and during pregnancy (R-BP), during lactation (R-L) or throughout the experimental period (R-BPL). At day 14 of lactation, maternal plasma albumin and vitamin A, liver iron stores and carcass fat were reduced in the restricted animals (R-L, R-BPL). Vitamin A stores were lowest in the chronically malnourished rats and were not replenished by refeeding during lactation. Milk yield was compromised in the restricted rats; the acutely malnourished rats (R-L) were much more severely affected than those chronically underfed (R-BPL). The restricted animals (R-L, R-BPL) only partially compensated by producing a more nutrient-dense milk. As a result, only total daily output of milk lactose and iron were reduced in the restricted rats. Thus, nutritional insults occurring both before and during lactation influenced lactational performance. The negative effects of chronic malnutrition were largely overcome by refeeding during lactation.     

Bordetella pertussis FHA antibodies in maternal/infants sera and colostrum.

The high incidence of pertussis in the first year of life confirms that susceptibility remains high for children in this age group despite > 90% pertussis vaccine compliance. In this respect, immunoresponse to Bordetella pertussis was investigated. Filamentous hemagglutinin (FHA) antibodies were studied due to their important protective role, in blocking the adherence of the bacteria to respiratory tract ciliated cells. The relative rate of detection and degree of positivity of IgG and IgA antibodies to Bordetella pertussis FHA were studied in maternal and infant sera and in colostrum samples of the respective mothers. The study comprised 143 mothers of child bearing age and 25 newborns. The highest percentages of serum IgG and IgA were present in the younger females group (15-25 yrs). Both IgG and IgA were detected in the same mother in 60% of them. The study showed that 96.9% of colostrum samples who were positive for IgA, were associated with IgA positivity in serum, also an increase in the degree of serum IgA positivity was associated with a higher rate of detection of IgA in colostrum. Maternal serum IgA could therefore be used as a marker for the future presence of IgA in colostrum. This work demonstrated that newborns show little passive immunity to pertussis, evidenced by the low placental transfer of IgG (35.7%) and the low rate of detection of IgA in the colostrum (41%). We concluded that, it would be advantageous to reimmunize pregnant women, without adequate serum antibody to Bordetella pertussis, with appropriate new vaccine which would offer a better passive immunity to their infants.     

Milk immunoglobulins and complement factors

The importance of colostrum for the growth and health of newborn offspring is well known. In bovine colostrum, the antibody (immunoglobulin) complement system provides a major antimicrobial effect against a wide range of microbes and confers passive immunity until the calf's own immune system has matured. Bovine serum and lacteal secretions contain three major classes of immunoglobulins: IgG, IgM and IgA. The immunoglobulins are selectively transported from the serum into the mammary gland, as a result of which the first colostrum contains very high concentrations of immunoglobulins (40-200 mg/ml). IgG1 accounts for over 75 % of the immunoglobulins in colostral whey, followed by IgM, IgA and IgG2. All these immunoglobulins decrease within a few days to a total immunoglobulin concentration of 0.7-1.0 mg/ml, with IgG1 representing the major Ig class in milk throughout the lactation period. Together with the antibodies absorbed from colostrum after birth, the complement system plays a crucial role in the passive immunisation of the newborn calf. The occurrence of haemolytic or bactericidal complement activity in bovine colostrum and milk has been demonstrated in several studies. This review deals with the characteristics of bovine Igs and the complement system to be exploited as potential ingredients for health-promoting functional foods.     

Maternal antibodies to rotavirus: could they interfere with live rotavirus vaccines in developing countries?

Introduction

Past experience with live oral vaccines including licensed rotavirus vaccines demonstrates a trend towards reduced vaccine efficacy in developing countries compared with developed countries. The reasons behind this disparity are not well understood. Transplacental transfer of maternal antibodies and breast milk ingestion may attenuate vaccine responses in infants in developing countries where rotavirus infections are endemic, and maternal antibody levels are high. We examined the prevalence and level of rotavirus antibody in maternal and cord serum, colostrum and breast milk in a developing country setting.

Methods

100 mother-infant pairs were prospectively recruited from December 2008 to February 2009 at Dr. Sardjito Hospital, Yogyakarta, Indonesia. Maternal and cord sera were collected during delivery. Colostrum and transitional breast milk were collected between day 0-3 and day 7-10 postpartum respectively. Rotavirus-specific IgA and IgG were estimated for all specimens and virus neutralization assays were conducted on a subset of milk specimens.

Results

All maternal and cord serum samples were positive for rotavirus-specific IgG antibodies with a strong correlation between levels of rotavirus-specific IgG in mothers and levels transferred to infants in cord blood (r = 0.86; p = 0.001). 78% of colostrum and 67% of transitional breast milk specimens were positive for rotavirus-specific IgA. There was a median 4-fold decrease in rotavirus-specific IgA from colostrum to transitional breast milk. Neutralizing antibodies were present in 56% of colostrum specimens assayed (19/34) and in 41% of transitional milk specimens assayed (14/34).

Conclusions

Maternal serum and breast milk antibodies to rotavirus are highly prevalent in a developing country setting. Evaluation of the impact of maternal anti-rotavirus serum and breast milk antibody upon vaccine immunogenicity would help to inform rotavirus vaccination strategies, especially in developing settings.     

Prolonged impairment of cell-mediated immunity after major abdominal surgery in malnourished patients

The effect of the nutritional status on postoperative impairment of the immune response was studied in adults undergoing major abdominal surgery. The immune function was evaluated by measuring in vitro the lymphocytic response to phytohaemagglutinin (PHA), concanavalin A (Con A) and the purified protein derivative of tuberculin (PPD) in whole blood cultures, and in vivo delayed skin hypersensitivity to candida, mumps, streptokinase-streptodornase and PPD. Nutritional assessment was carried out by evaluating recent weight loss, the weight for height index and by measuring the arm muscle circumference (AMC), triceps skinfold thickness (TSF), the creatinine-height index (CHI) and the serum concentration of albumin and prealbumin. The patient was considered malnourished, if at least three of these criteria were abnormal. The immune parameters were measured preoperatively, at the end of the surgery and five days after operation. Before the operation both malnourished and well-nourished patients had normal lymphocytic responses, but the malnourished patients had a slower recovery of immune responses after the operation and they had an increased number of postoperative complications. No significant differences in the incidence of anergy were observed between the well and malnourished patients pre or postoperatively.     

Trans fatty acids in human milk lipids: influence of maternal diet and weight loss

Geometric and positional isomers of octadecanoic acid in milk expressed during the first 9 weeks of lactation were determined for mothers delivering prematurely (PT) and at term (FT). Total C18:1 concentration was lower in PT than FT milk. C18:1 trans (elaidic acid) content was higher in PT and FT colostrum compared with subsequent milk samples. No relationship was observed between C18:1 trans level in milk and oleic acid, linoleic acid, total fatty acid levels, or volume of milk expressed. While gestational age and lactational stage appeared to affect the trans fatty acid pattern, the overriding influence was rate of maternal postpartum weight loss. Effect of weight loss on milk trans fatty acid levels was independent of maternal diet. Trans fatty acid content of milk as a function of recent maternal fat intake was also demonstrated. This study indicates that infants receiving human milk ingest levels of trans fatty acids reflecting short and long term maternal diet.     

Lysozyme, lactoferrin, and secretory immunoglobulin A content in breast milk: influence of duration of lactation, nutrition status, prolactin status, and parity of mother

Milk lactoferrin (LF), lysozyme (LZ), and secretory IgA (sIgA) were measured cross-sectionally in 127 Za?rean mothers, lactating greater than or equal to 18 mo. The 54 urban mothers were of marginal nutrition status [body mass index (BMI) 22.6 +/- 2.6 kg/m2 and albumin 33.1 +/- 4.5 g/L]. The neighboring rural mothers were of significantly (P less than 0.001) poorer nutrition status (BMI 20.5 +/- 2.2 kg/m2 and albumin 27.7 +/- 5.4 g/L). In both urban and rural mothers, as lactation progressed LF decreased by 33% and 55% whereas sIgA remained unchanged and LZ steadily increased. There was more LZ and sIgA in rural milk, contrasting with the poorer maternal nutrition. As calculated from individual milk yields, the urban infants were fed daily with twice as much LF and sIgA but with similar amounts of LZ as were the rural infants. In the early stage of lactation, the milk of both groups of Za?rean mothers contains more sIgA than that of a group of west European (Belgian) mothers (n = 20), but the LF and LZ contents were rather similar.     

Nutritional status and lymphocyte function in maintenance hemodialysis patients

Nutritional status and lymphocyte transformation were examined in 30 clinically stable men who underwent maintenance hemodialysis for 1 to 141 months. The men displayed decreased relative body weight, triceps and subscapular skinfold thickness, midarm circumference, midarm muscle circumference, serum total protein, albumin, transferrin, IgG, IgA, IgM, and C3 concentrations. There were many abnormalities in the plasma amino acid pattern. Lymphocyte transformation stimulated by phytohemagglutin or pokeweed mitogen was reduced. Many nutritional parameters correlated with each other and with the rate of lymphocyte transformation. There was a tendency (p = 0.06) for higher mortality in the malnourished patients during a mean follow-up period of 43.3 months. These findings suggest that clinically stable men undergoing maintenance hemodialysis are typically malnourished. Poor nutritional status may be a cause of impaired lymphocyte function. Malnutrition or wasting may indicate that the patient is at risk for a higher mortality rate.     

Quantitation of the major whey proteins in human milk,and development of a technique to isolate minor whey proteins

Several whey proteins of human milk have important nutritional and physiological roles for the breast fed infant. Only 75 to 85% of the proteins in whey have been identified and quantitated, leaving a remaining protein fraction containing many proteins of probable nutritional and physiological significance. In this study, colostrum and mature milk samples were collected, and the concentrations of five major whey proteins were measured using immunoelectrophoresis or immunodiffusion. Based on Kjeldahl nitrogen analysis, the total concentration of whey proteins in colostrum was 17.05 mg/ml and in mature milk 5.95 mg/ml. The concentration of α-lactalbumin was 3.7 mg/ml and 1.7 mg/ml in colostrum and mature milk, respectively; lactoferrin concentration was 7.3 and 1.5 mg/ml; and secretory IgA concentration was 4.7 and 1.2 mg/ml. In both colostrum and mature milk, concentrations of serum albumin and lysozyme were 0.37 and 0.07 mg/ml, respectively. The sum of these major whey proteins expressed as a percentage of the total whey protein accounted for 81% of mature whey protein, and 94% of colostral whey protein, leaving 19% and 6% in the remaining protein fraction. A method was developed to isolate this remaining protein fraction from the major whey proteins using gel filtration and immunoaffinity chromatography. The quantitatively minor whey protein fraction obtained was further characterized using polyacrylamide gel electrophoresis and ion exchange chromatography.     

Impact of Maternal Daily Oral Low-Dose Vitamin A Supplementation on the Mother-Infant Pair: A Randomised Placebo-Controlled Trial in China

Background: The nutritional status of vitamin A in lactating mothers and infants is still not optimistic. Due to the dietary habits and dietary restrictions of postpartum customs in China, vitamin A supplementation has been advocated as a potential strategy to improve vitamin A status of lactating mothers with inadequate dietary vitamin A intake. Existing clinical trials are limited to single or double high-dose maternal administrations. However, in China, vitamin A supplements are readily available in the form of daily oral low-dose supplements, and the effect of these is unknown. This study aimed to evaluate the effects of daily oral low-dose vitamin A supplementation on the retinol levels in the serum and breast milk of lactating mothers and the health status of infants in China. Methods: Lactating mothers who met the inclusion criteria and planned to continue exclusive breastfeeding were randomly assigned to receive either daily oral vitamin A and D drops (one soft capsule of 1800 IU vitamin A and 600 IU vitamin D₂), or a matching placebo for 2 months. Before and after the intervention, dietary intake was investigated by instant photography, and the retinol concentration in maternal serum and breast milk was determined by ultra-high performance liquid chromatography-tandem mass spectrometry. During the trial, the health status of infants was diagnosed by a paediatrician or reported by lactating mothers. A total of 245 participants completed the study, with 117 in the supplementation group and 128 in the control group. Results: After the 2-month intervention, maternal serum retinol concentrations increased in the supplementation group with no change in the control group. Although breast milk retinol concentrations decreased significantly in both groups, the decrease in the supplementation group was significantly lower than that in the control group. However, maternal vitamin A supplementation was not associated with a lower risk of infant febrile illness, respiratory tract infection, diarrhoea, and eczema. Conclusions: Daily oral low-dose vitamin A supplementation is helpful in improving maternal vitamin A status, despite having no effect on infant health status through breast milk.     

Response to total parenteral nutrition in the extremely malnourished patient

Changes in serum albumin levels and body weight are often used as indicators of the efficiency of a nutritional support regimen. Patients with moderate nutritional depletion demonstrate two distinct patterns of response during refeeding. The first is characterized by a decrease in the previously expanded extracellular fluid space with a rise in serum albumin and a loss of weight and the second by continued fluid retention with weight gain and no rise in serum albumin concentration. The second pattern has been observed in patients with ongoing stress such as infection. This study examines severely malnourished patients with no apparent inflammatory complications and demonstrates that this group responds to nutritional support in a pattern similar to that seen in the stressed patient. Eight patients with profound malnutrition were studied during the 1st week of nutritional support. Nitrogen balance was measured and the findings confirmed that all patients were anabolic. Sodium balances were used as an indicator of changes in the extracellular fluid compartment. Body weight and serum albumin were assessed daily. Body weight increased from 59 +/- 4 to 62 +/- 4% of normal (p less than 0.01) while serum albumin changed insignificantly (3.00 +/- 0.27 to 2.85 +/- 0.23 g/100 ml, NS) during the initial week of an adequate nutritional support regimen (nitrogen balance was +21.0 +/- 4.3 g, p less than 0.05). These changes were associated with a positive sodium balance (+215 +/- 20 mEq, p less than 0.05). These data confirm that some extremely malnourished patients do not experience a diuresis during the initial phase of nutritional support but rather may retain water and increase body weight.(ABSTRACT TRUNCATED AT 250 WORDS)     

肠内免疫营养对胃癌手术病人免疫功能和急性炎性反应的调理作用

目的 :观察胃癌病人围手术期使用肿瘤特异性肠内免疫营养物 Supportan对病人的营养、免疫和急性炎性反应的调理作用。　方法 :本研究是一个前瞻、随机、单盲、对照的临床研究。30例营养不良择期手术的胃癌病人 ,随机分为两组 :1、免疫营养组 (Supportan,n=15 ) ,2、标准营养组 (能全素 Nutrison,n=15 )。两组使用等热量肠内营养 ,12 5 k J(30 kcal) / (kg· d)。术前置鼻胃管 ,使用 7天。术中均行空肠造口 ,术后 12小时开始空肠输注肠内营养 ,连续 7天。术前第 8、1天 ;术后第 1、8天抽取静脉血 ,检测血清白蛋白、前白蛋白、转铁蛋白、T淋巴细胞亚群、血清免疫球蛋白、血清 IL - 6、CRP、PGE2 浓度。　结果 :两组病人术后均有急性炎性反应的发生和免疫功能的降低。但免疫营养组比标准营养组的术后第 1、8天的 CD4/ CD8比值、血清 Ig G水平有显著增加 ;血清 IL- 6、CRP、PGE2 有显著下降 ;术后第 8天血清前白蛋白浓度有显著增加。　结论 :胃癌合并营养不良的病人围手术期使用肠内免疫营养物 Supportan可以改善手术后免疫功能的低下 ,缓解急性相炎性反应和增加内脏蛋白的合成。     

Rotavirus specific maternal antibodies and immune response to RV3-BB rotavirus vaccine in central java and yogyakarta,Indonesia

BackgroundPlacental or breast milk maternal antibodies can potentially reduce oral rotavirus vaccine efficacy in developing countries. We aimed to examine the relationship between the level of rotavirus specific immunoglobulin A (IgA) and neutralising antibodies (NA) in colostrum and breast milk and cord IgG, with cumulative vaccine take following one and three doses of oral RV3-BB rotavirus vaccine within a Phase IIb trial in Indonesia.Methods196 infants received three doses of RV3-BB in a randomized, double-blinded trial, using a neonatal schedule (first dose at 0–5 days of age, n = 61), an infant schedule (first dose at ~ 8 weeks of age, n = 67) or placebo (n = 68). Rotavirus specific IgA and NA in colostrum and breast milk, rotavirus specific cord IgG, Serum IgA and stool excretion were measured.ResultsThere was little evidence of an association between IgA in colostrum or breast milk and cumulative vaccine take after three doses in the neonatal or infant groups. In the neonatal group, there was a negative association between IgG titre in cord blood and cumulative vaccine take (odds ratio [OR] 0.96; 95% confidence interval [CI] 0.92–1.00; p = 0.03) and serum IgA response (OR 0.94; 95%CI 0.89–0.99; p = 0.02) after one dose of vaccine, which were not evident after three doses in the neonatal or infant groups.ConclusionsAmongst Indonesian infants we did not find an association between IgA in colostrum or breast milk and vaccine take after 3 doses of RV3-BB vaccine. Maternal rotavirus antibodies in breast milk appear to have minimal impact on RV3-BB vaccine take when administered with a short delay in breast-feeding in settings with a high rotavirus disease burden.