多肽对生物材料表面修饰的研究现状

对生物材料进行表面修饰可以提高生物材料的细胞粘附性，用多肽对生物材料进行表面修饰是一种较好的表面改性方法。本文对生物材料表面修饰用多肽的种类的选择及多肽固定的方法研究现状进行综述。     

不同多肽修饰方法对纯钛微弧氧化膜层性能的影响

背景：理论上推测钛基-微弧氧化陶瓷膜-精氨酸-甘氨酸-天冬氨酸(RGD)序列多肽的结合模式应具较好的力学和生物学性能。 目的：观察不同修饰方法固定RGD多肽后,钛基体微弧氧化膜层表面的微观结构和细胞增殖。 方法：取纯钛与微弧氧化纯钛试件共90枚,分3种方法固定RGD多肽,分别为RGD多肽物理吸附修饰纯钛组、RGD多肽物理吸附修饰微弧氧化组与RGD多肽化学偶联修饰微弧氧化组,每组30枚。应用荧光显微镜观察3组试件表面接枝效果,采用X射线光电子能谱扫描检测试样表面的RGD多肽含量。将3组试件分别与小鼠骨髓基质细胞培养,光镜观察各时间点的细胞黏附及增殖情况。 结果与结论：3组试样表面有大小不一、数量不等的绿色荧光亮点,在单位视野中,RGD多肽化学偶联修饰微弧氧化组荧光最强,提示此组试件接枝了更多的多肽。RGD多肽物理吸附修饰纯钛组试样表面仅含少量或微量多肽,RGD多肽物理吸附修饰微弧氧化组含多肽量居中,RGD多肽化学偶联修饰微弧氧化组含肽量最高。3组试件均无明显的细胞毒性,但RGD多肽化学偶联修饰微弧氧化组细胞生长情况最好。表明化学偶联法可以较好地将RGD多肽固定在含微弧氧化膜层的纯钛试样表面,无明显细胞毒性,有利于细胞的生长增殖。中国组织工程研究杂志出版内容重点：生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程全文链接：     

人工韧带表面修饰重建韧带缺损的方法及其效果评价

目的:综述生物材料治疗韧带缺损中表面修饰的主要方法及其效果,为科研工作者提供一定的借鉴。方法:采用电子检索的方式,在万方数据库(http://www.wanfangdata.com.cn/)中检索2000-01/2010-05关于韧带重建生物材料表面修饰方法与应用效果的研究文章,关键词为"生物材料,表面修饰,人工韧带"。排除重复研究、普通综述或Meta分析类文章,筛选纳入22篇文献进行评价。结果:目前为了使生物材料在治疗韧带、肌腱等缺损中具有良好的生物相容性和细胞亲和性,要进行生物材料表面改性,通常称为表面修饰。所采用的表面修饰方法主要有固定蛋白质法、固定多肽法、辐射接枝法、离子注入法。研究表明,通过生物材料表面修饰,不仅增进了细胞的黏附,而且促进了细胞的增殖。结论:生物材料的表面修饰是一个复杂的系统工程,需要兼顾材料科学和生物科学的需要,实现优化。理想的表面修饰应该兼顾表面拓扑结构、特异性识别、亲水/疏水平衡、蛋白质吸附等各个方面才能得到功能化的新生组织。     

组织工程中生物材料表面修饰的研究

为了提高生物材料的生物相容性和细胞亲和性 ,需对生物材料进行表面修饰。本文对生物材料表面修饰的方法进行了综述 ,并提出今后尚待解决的问题和发展趋势     

组织工程中生物材料表面修饰的研究

为了提高生物材料的生物相容性和细胞亲和性，需对生物材料进行表面修饰。本对生物材料表面修饰的方法进行了综述，并提出今后尚待解决的问题和发展趋势。     

RGD肽表面修饰聚苯乙烯及其细胞相容性研究

目的以聚苯乙烯二维平面为模板研究了蛋白表面修饰技术,构建具有生物活性的生物材料表面。方法采用物理包被法依靠疏水作用在PS表面架构明胶、胶原和RGD（精氨酸-甘氨酸-天冬氨酸）多肽的生物活性层。通过光电子能谱（XPS）分析修饰表面的元素含量变化,N元素含量显著提高,说明蛋白分子在表面存在。Bradford方法定量分析明胶、胶原和RGD多肽的表面吸附量。结果XPS证实了表面N原子的引入,存在酰胺键,确定蛋白分子存在于PS表面。结论动态接触角下降显著,证明修饰表面的亲水性得到提高。并在明胶、胶原和RGD多肽修饰表面接种人表皮细胞,对比考察其对细胞行为的影响,提高了细胞的黏附和增殖能力。     

表面修饰对羟基磷灰石修复骨缺损的影响

精氨酸-甘氨酸-天冬氨酸（Arg-Gly-Asp,RGD）多肽是介导种子细胞与支架材料黏附的多肽链．RGD序列可被固有黏附蛋白受体特异性结合．在生物材料表面自发形成一分子层．为与受体介导的种子细胞提供特异性位点而促进细胞的黏附和分化。笔者旨在应用RGD多肽对羟基磷灰石（hydroxyapatite．HA）材料进行表面修饰处理．以促进骨髓基质干细胞（marrow stromal cells．MSC）在其表面的黏附和生长．为骨组织工程提供一种支架材料的表面修饰手段。     

生物材料的表面修饰对细胞黏附性的影响

细胞与生物材料相互作用过程中,细胞黏附是首先发生的生物学行为,对后续发生的迁徙、增殖和分化具有重要影响.因此,如何改善细胞在生物材料表面的黏附特性,自然成为目前组织工程研究的一项重要内容,目前较多采用的是对生物材料进行表面修饰的方法.就生物材料的表面修饰对细胞在生物材料上黏附的影响进展作一综述.     

人工韧带材料表面修饰及在膝关节交叉韧带重建中的应用

目的:综述人工韧带表面修饰技术及其在重建膝关节交叉韧带中的效果。方法:采用电子检索的方式,在万方数据库(http://www.wanfangdata.com.cn/)中检索2000-01/2010-08关于人工韧带表面修饰技术及其在重建膝关节交叉韧带应用方面的文章,关键词为"人工韧带,表面修饰,交叉韧带"。排除重复研究、普通综述或Meta分析类文章,筛选纳入33篇文献进行评价。结果:生物材料的表面修饰是一项复杂的系统工程,需要兼顾材料学和生物科学的需要,实现理想的表面修饰应该设计表面拓扑结构-特异性识别-亲水/疏水平衡-蛋白质吸附等各个方面。目前所采用的表面修饰技术主要有固定多肽法、固定蛋白质法、激光表面修饰法、辐射接枝法、离子注入法、接枝改性法。研究表明,通过生物材料表面修饰,不仅增进了细胞的黏附,而且促进了细胞的增殖。结论:人工韧带表面修饰技术在提高人工材料的生物相容性方面取得了显著效果,具有良好的应用前景。     

肝素固定化技术研究进展

生物材料表面肝素固定化修饰技术是改善生物材料表面抗凝性能和生物相容性的一种有效方法。本文针对生物材料表面肝素固定化修饰技术的原理思想、实验研究和运用等方面做一综述,并展望了肝素固定化技术的前景。     

多肽修饰聚合物PLGA-[ASP-PEG]对骨髓基质细胞黏附特性的影响

探讨在PLGA-[ASP-PEG]表面进行多肽改性后,对骨髓基质细胞在其表面黏附力的影响。在骨支架材料PLGA-[ASP-PEG]表面固定多肽GRGDSPC,用微吸管吸吮法测定骨髓基质细胞不同的时间段在骨支架材料表面的黏附力,并进行扫描电镜观察。结果表明:骨髓基质细胞接种在二种支架材料上4 h时,PLGA-[ASP-PEG]表面黏附力为172.78±15.23 N,多肽改性的PLGA-[SP-PEG]细胞黏附力209.47±92.59 N,二者无明显差异;在12h,多肽改性的PLGA-[ASP-PEG]黏附力576.23±165.74 N,PLGA-[ASP-PEG]黏附力为261.84±100.09 N,前者表面细胞黏附力明显强于后者(P<0.01);在24 h时,二种材料表面的细胞黏附力无明显差异(P>0.05)。扫描电镜观察结果为多肽改性支架材料上表面黏附的细胞数明显多于未改性材料表面黏附的细胞数。在生物材料表面结合多肽可以增强细胞在材料表面的黏附力,从而改善生物材料生物相容性。     

生物医用金属材料表面改性研究

本文主要对生物医用金属材料表面改性的几种方法进行了综述,并对表面改性在生物金属材料方面的应用作出了展望.     

成骨细胞在生物活性材料中黏附性能研究进展

成骨细胞与骨基质材料间的相互作用是骨组织工程研究的主要领域,其中细胞与材料的黏附是基础,细胞必须与材料发生适当的黏附,才能进行迁移、增殖和分化。综述了与成骨细胞黏附有关的蛋白质、生物活性复合材料的表面特征和表面修饰对成骨细胞黏附性能的影响,为骨组织工程的研究提供一定的依据,尤其为组织工程新材料的研制提供参考。     

Human recombinant elastin-like protein coatings for muscle cell proliferation and differentiation

Recombinant proteins represent a new and promising class of polymeric materials in the field of biomaterials research. An important model for biomaterial design is elastin, the protein accounting for the elasticity of several tissues. Human elastin-like polypeptides (HELPs) have been developed as recombinant versions of elastin with the purpose of enhancing some peculiar characteristics of the native protein, like self-assembling. In this paper, we report on a comparative study of rat myoblasts response to coatings based on two different HELP macromolecules, with respect to control cultures on bare cell culture polystyrene and on a standard collagen coating. Cell behavior was analyzed in terms of adhesion, proliferation and differentiation. The collected data strongly suggest the use of HELPs as excellent biomaterials for tissue engineering and regenerative medicine applications.     

植入用高分子材料表面改性抗细菌粘附的研究进展

以植入材料为中心的感染导致的手术失败，严重限制了临床生物医用材料的应用。本文介绍了有关细菌在材料表面粘附的理论，材料表面自由能与细菌材料表面粘附的关系，并综述了近年来生物医用材料的表面改性的多种方法。     

Fibroblast cell attachment and growth on nanoengineered sculptured thin films

Nanoengineered parylene-C sculptured thin films (STFs) are deposited on glass and silicon substrates using a direct one-step growth technique. The deposited STFs support fibroblast cell attachment and proliferation in vitro, which is an early indication of biocompatibility and bioactivity of this emerging class of biomaterials. Surface modification of endoprostheses of the small joints of the hand, which heal with fibrous stabilization, may be greatly enhanced by such nanoengineered biomaterials.     

Biomimetic approaches to modulate cellular adhesion in biomaterials: A review

Natural extracellular matrix (ECM) proteins possess critical biological characteristics that provide a platform for cellular adhesion and activation of highly regulated signaling pathways. However, ECM-based biomaterials can have several limitations, including poor mechanical properties and risk of immunogenicity. Synthetic biomaterials alleviate the risks associated with natural biomaterials but often lack the robust biological activity necessary to direct cell function beyond initial adhesion. A thorough understanding of receptor-mediated cellular adhesion to the ECM and subsequent signaling activation has facilitated development of techniques that functionalize inert biomaterials to provide a biologically active surface. Here we review a range of approaches used to modify biomaterial surfaces for optimal receptor-mediated cell interactions, as well as provide insights into specific mechanisms of downstream signaling activation. In addition to a brief overview of integrin receptor-mediated cell function, so-called “biomimetic” techniques reviewed here include (i) surface modification of biomaterials with bioadhesive ECM macromolecules or specific binding motifs, (ii) nanoscale patterning of the materials and (iii) the use of “natural-like” biomaterials.     

Cell death in HeLa mediated by thermoplastic polyurethane with co-immobilized IFN-γ plus TNF-α

In order to prohibit the toxicity of free IFN-γ plus TNF-α in treating human cervical cancer HeLa cells, two kinds of thermoplastic polyurethane (polyester/polyether) biomaterials with co-immobilized IFN-γ plus TNF-α on the surfaces are prepared. The programmed cell death of HeLa induced by these biomaterials is investigated. The surface modification of these biomaterials with co-immobilized IFN-γ plus TNF-α is performed by the photo-immobilization method, and the surface structures are characterized by various techniques. The cell morphology, cell mortality, cell cycle arrest, and functional status of caspases, upon the treatment by these biomaterials, are characterized. The results show that the as-prepared biomaterials have high inhibition activity against the growth of HeLa cells. The HeLa cells mediated by the two kinds of biomaterials are mainly arrested in the G(1) phase, while those cells mediated directly by free IFN-γ plus TNF-α are mainly arrested in the S phase. It is suggested that the programmed cell death mechanism induced by these two kinds of biomaterials is both caspase-dependent and caspase-independent. Our data provide the knowledge of microscopic surface structures and cell biology basis for synthesizing the thermoplastic polyurethane biomaterials with co-immobilized IFN-γ plus TNF-α, which are promising for novel therapeutics (e.g. drug cup) design for cervical cancer patients.     

The use of air plasma in surface modification of peripheral nerve conduits

Surface modification is a conventional approach in biomaterials development, but most of the modification processes are intricate and time inefficient. In this study, a convenient open air plasma treatment was employed to modify the surface of poly(d,l-lactide) (PLA). Chitosan and fibroblast growth factor 1 (FGF1) were sequentially grafted with the assistance of open air plasma treatment onto the PLA nerve conduits with designed micropores and surface microgrooves. Grafting of these components was verified by electron spectroscopy for chemical analysis. The modified nerve conduits showed enhanced ability in the repair of 10-mm sciatic nerve transection defects in rats. The sequential air plasma treatment can be a convenient way to introduce biocompatible (e.g., chitosan) and bioactive components (e.g., growth factors) onto the surface of biomaterials.