六种免疫相关疾病血清sIL—2Ra水平

本文研究了一组免疫相关疾病，如系统性红斑狼疮，干燥综合症，非何杰金氏淋巴瘤，类风湿性关节炎，Ｇｒａｖｅｓ＇病和头颈部肿瘤患者血清ｓＩＬ－２Ｒａ水平，并与正常人对照组作了比较。结果显示上述各病种患者血清ｓＩＬ－２Ｒａ明显增高。与正常人血清ｓＩＬ－２Ｒａ水平比较均存在极显著性差异。本文还就ｓＩＬ－２Ｒａ的临床意义和应用前景等若干问题进行了讨论。     

血清可溶性IL-2R检测在结缔组织病中的应用

应用夹心法ELISA检测了正常人、混合性结缔组织病(MCTD)、系统性红斑狼疮(SLE)、类风湿性关节炎(RA)、系统性硬皮病患者血清可溶性白细胞介素-2受体(SIL-2R)水平。结果表明MCTD、SLE、RA患者血清SIL-2R水平明显高干正常对照组(P<0.001);对部分患者血清SIL-2R进行了动态观察,发现结缔组织病患者,随着病情的改善,血清SIL-2R水平降低。本文结果提示,结缔组织病患者存在着淋巴细胞激活状态,检测血清SIL-2R可能对结缔组织病患者活动性观察有一定意义,可作为衡量体内细胞免疫活性的指标。     

新生儿脐血T细胞IL－2R表达和血清sIL－2R水平的研究

对新生儿脐血Ｔ细胞ＩＬ－２Ｒ表达率和血清ｓＩＬ－２Ｒ水平进行检测，结果显示：①早产儿脐血Ｔ细胞ＩＬ－２Ｒ自然表达率明显低于足月新生儿和正常儿童组，而后两者间无差异；②经ＰＨＡ活化后足月新生儿和早产儿Ｔ细胞ＩＬ－２Ｒ表达率皆显著低于正常儿童组，早产儿组也明显低于足月新生儿组；③足月新生儿和早产儿脐血血清ｓＩＬ－２Ｒ水平均明显高于正常儿童组，但前两者间无明显差异；④新生儿组（包括足月和早产儿）脐血Ｔ细胞ＩＬ－２Ｒ自然表达率与血清ｓＩＬ－２Ｒ水平呈直线负相关，而正常儿童组二者间呈直线正相关。     

慢性肾炎患者sIL－2R水平、IL－2活性及mIL－2R表达的观察

本文应用ＥＬＩＳＡ法检测了４０例慢性肾小球肾炎血清可溶性白细胞介素２受体水平，同时对患者外周血单个核细胞膜白细胞介素２受体表达及白细胞介素２活性进行观察。结果患者ｓＩＬ－２Ｒ水平为６３４．８±１４２．９ｕ／ｍｌ，高于正常人２９５．０±１６５．７ｕ／ｍｌ，Ｐ＜０．００１；ｍＩＬ－２Ｒ阳性率为２５．６±４．３％，低于正常人４５．５±５．２％，Ｐ＜０．００１；ＩＬ－２活性为２．８５±１．６１ｕ／ｍｌ，低于正常人７．０６±４．５３ｕ／ｍｌ，Ｐ＜０．００１。并且ｓＩＬ－２Ｒ与ＢＵＮ呈正相关，ｒ＝０．４７０，Ｐ＜０．０２。提示慢性肾小球肾炎患者细胞免疫功能降低，且与肾功能损伤程度有关。     

血清sIL—2R检测在肺癌中的应用

可溶性白介素２受体（ｓＩＬ－２Ｒ）与白介素２（ＩＬ－２）介导的免疫反应密切相关，检测ＳＩＬ－２Ｒ取材方便，易于检测，已迅速引起临床医学重视。肺癌病人血清ｓＩＬ－２Ｒ升高，对治疗有反庆者降低，是肺癌病人病情监测，疗效判定的参考指标。本文就ｓＩＬ－２Ｒ在肺癌方面的临床应用研究作一综述。     

恶性肿瘤和感染性疾病患者血清sIL－2R的动态观察

本文对恶性肿瘤和感染性疾病患者血清中ｓＩＬ－２Ｒ水平进行动态观察。结果表明，手术切除恶性肿瘤后，发生转移的患者血清ｓＩＬ－２Ｒ水平较手术前升高；未发生转移的患者ｓＩＬ－２Ｒ水平较手术前降低，提示术后ｓＩＬ－２Ｒ水平的升高可能与肿瘤的转移有关。ｓＩＬ－２Ｒ与其它癌指标同步检测结果显示，ｓＩＬ－２Ｒ水平的异常升高比其它癌指标出现得早。ｓＩＬ－２Ｒ水平与感染性疾病的活动程度有关。相关分析表明，ｓＩＬ－２Ｒ与ＨＡ和ＣＧ呈显著正相关。     

人巨细胞病毒感染对肾移植受者血清sIL－2R水平的影响

用ＰＣＲ检测ＨＣＭＶ－ＤＮＡ，ＥＬＩＳＡ法检测ＨＣＭＶ－ＩｇＭ及ＩｇＧ，以诊断肾移植受者ＨＣＭＶ感染。用双抗体夹心法ＥＬＩＳＡ检测６５例肾移植受者血清ｓＩＬ－２Ｒ水平，结果表明：ＨＣＭＶ感染后宿主血清ｓＩＬ－２Ｒ水平明显增高（Ｐ＜０．０１），且ＨＣＭＶ疾病组ｓＩＬ－２Ｒ增高程度大于无症状感染组（Ｐ＜０．０１）；６例原发性ＨＣＭＶ感染者ｓＩＬ－２Ｒ水平与ＩｇＭ水平呈正相关（ｒ＝０．９９０８），提示随感染程度增加，血清ｓＩＬ－２Ｒ水平随之增高，还发现血清ｓＩＬ－２Ｒ水平与Ｃ９４／ＣＤ８比值是负相关（ｒ＝－０．９７８９），说明ＨＣＭｖ感染后ｓＩＬ－２Ｒ水平增高与Ｔ细胞亚群改变有关，反之也说明ｓＩＬ－２Ｒ增高程度可表明体内免疫抑制状态。对于ＨＣＭＶ感染后血清ｓＩＬ－２Ｒ水平增高的机理有待进一步探讨。     

恶性肿瘤和感染性疾病患者血清sIL－2R的动态观察

本文对恶性肿瘤和感染性疾病患者血清中ｓＩＬ－２Ｒ水平进行动态观察。结果表明，手术切除恶性肿瘤后，发生转移的患者血清ｓＩＬ－２Ｒ水平较手术前升高；未发生转移的患者ｓＩＬ－２Ｒ水平较手术前降低，提示术后ｓＩＬ－２Ｒ水平的升高可能与肿瘤的转移有关。ｓＩＬ－２Ｒ与其它癌指标同步检测结果显示，ｓＩＬ－２Ｒ水平的异常升高比其它癌指标出现得早。ｓＩＬ－２Ｒ水平与感染性疾病的活动程度有关。相关分析表明，ｓＩＬ－２Ｒ与ＨＡ和ＣＧ呈显著正相关。     

肝硬化患者血清中一氧化氮和sIL2R水平的变化

为了解肝硬化患者血清中ＮＯ和ｓＩＬ 2Ｒ的水平变化与肝硬化病情演变过程的相互关系 ,本文检测了 43例肝硬化患者血清中ＮＯ和ｓＩＬ 2Ｒ的水平 ,并对两者作直线相关分析。1　材料和方法1 1　研究对象　病毒性乙型肝炎后肝硬化失代偿期患者 43例 ,均龄 39 8岁 ;同期检测急性肝炎 2 1例 ,慢性肝炎 2 8例及正常人 30例。采血前无免疫制剂使用史 ,留取活动期与恢复期 2次血清标本 , 70℃保存集中检测。1 2　检测方法　ＮＯ采用硝酸还原酶法 ,试剂由南京聚力生物医学工程研究所提供 ;ｓＩＬ 2Ｒ用酶免疫法 ,试剂购自第四军医大学免疫学研究室。…     

抑郁症患者IL-2及sIL-2R的检测及临床意义

目的　探讨白介素 -2 ( IL-2 )及可溶性白介素 -2受体 ( s IL-2 R)在抑郁症发病中的作用及临床意义。方法　用酶联免疫吸附法检测 30例抑郁症患者和 30例正常人血清 IL-2及 s IL-2 R水平 ,并比较二者的差异。结果　抑郁症患者 IL-2及 s IL-2 R分别为 95 1 .2± 1 1 0 .5 ngl/L、( 389.6± 2 1 1 .1 ) U/ml,高于对照组的 384 .1± 72 .5 ng/L、2 83.6± 1 4 6 .7U/ml,二者比较差异有显著性 ( P<0 .0 1 )。结论　抑郁症患者 IL-2及 s IL-2 R水平增高。     

食管癌患者手术前后血清IL—2与SIL—2R水平的检测

采用ＭＴＴ比色法与双抗体夹心法对３８例食管癌患者进行了手术前后ＩＬ－２与ＳＩＬ－２Ｒ水平检测,并以正常人作对照。结果表明：食用癌患者手术前血清ＩＬ－２活性明显低于正常人,血清ＳＩＬ－２Ｒ水平明显地高于正常人;术后６个月复发者ＩＬ－２与ＳＩＬ－２Ｒ持续异常,未复发者ＩＬ－２与ＳＩＬ－２Ｒ水平恢复正常。观察了患者免疫功能的变化与肿瘤的发生及预后密切相关。     

Increased serum levels of soluble interleukin-2 receptor in patients with systemic lupus erythematosus and rheumatoid arthritis

In this study we investigated the serum levels of a released soluble form of the interleukin-2 receptor (sIL-2R) in 42 patients with rheumatoid arthritis and in 12 cases of systemic lupus erythematosus. Data were evaluated in relationship to the clinical phase and compared with those observed in normal controls (N=56) and in osteoarthritis (N = 7). Increased levels were observed in both rheumatoid arthritis (mean ± SE, 604±49 U/ml) and systemic lupus erythematosus (1438±481 U/ml). These values were significantly higher than in control (256±15 U/ml;P<0.001) and in osteoarthritis (298±33 U/ml;P<0.001) groups. In addition, the highest values were associated with the active phases of both rheumatoid arthritis (active vs inactive, 771±78 vs 451±39 U/ml;P<0.001) and systemic lupus erythematosus (active vs inactive, 2108±489 vs 499±75 U/ml;P<0.001). Our findings suggest that the detection of sIL-2R in rheumatoid arthritis and in systemic lupus erythematosus may represent a good marker of disease activity, which indirectly indicates the ongoing activation and/or proliferation of immunoreactive cells which are involved in the pathogenetic events of these autoimmune conditions.     

T-Helper 2 Type Cytokine and Soluble Interleukin-2 Receptor Levels in Seminal Plasma of Infertile Men

PROBLEM: The role of cell-mediated immunity (CMI) in unexplained male infertility and impaired sperm function has been explored. METHOD OF STUDY: The presence of cytokines, namely, interleukin-4 (IL-4), interleukin-6 (IL-6), and the soluble interleukin-2 receptor (SIL-2R), was investigated in seminal plasma of 18 fertile and 20 infertile subjects, using specific enzyme-linked immunoadsorbent assays. RESULTS: IL-4 was not detected. SIL-2R was detected, but the concentration difference between the fertile and infertile group was not significant. IL-6 was detected with significantly higher levels in the infertile group compared to the fertile group. IL-6 levels in seminal plasma correlated positively with leukocyte count and negatively with sperm count, motility, and morphology. CONCLUSIONS: These findings show: a) a lack of IL-4 in seminal plasma; b) similar SIL-2R levels in fertile and infertile seminal plasma; c) increased IL-6 secretion in seminal plasma of infertile subjects; and d) specific correlations of IL-6 with the main semen parameters.     

可溶性白细胞介素2受体在轮状病毒胃肠炎中价值的初探

动态观察轮状病毒胃肠炎患儿血浆中可溶性白细胞介素２受体（ＳＩＬ－２Ｒ）水平的变化，以及轮状病毒（ＲＶ）浓度与ＳＩＬ－２Ｒ，ＩＬ－２与ＳＩＬ－２Ｒ水平之间的关系。结果显示：急性期患儿血浆中ＳＩＬ－２Ｒ明显升高，恢复期患儿血浆中ＳＩＬ－２Ｒ接近正常；在ＲＶ抗原诱导下末梢血中单个核细胞可释放ＳＩＬ－２Ｒ；ＲＶ浓度与ＳＩＬ－２Ｒ水平呈负相关，ＩＬ－２水平与ＳＩＬ－２Ｒ亦呈负相关。提示：ＳＩＬ－２Ｒ分泌增高     

IL－2和sIL－2R在哮喘发病中的意义探讨

为了探讨ＩＬ－２和ｓＩＬ－２Ｒ在哮喘发病中的意义，对３６例哮喘患者外周血单个核细胞（ＰＢＭＣ）诱生ＩＬ－２水平和血浆ｓＩＬ－２Ｒ水平进行了检测，同时以支气管炎患者与正常人作对照。结果表明，ＰＢＭｃ诱生的ＩＬ－２活性哮喘组高于正常对照组（ｐ＜０．０５）；血浆ｓＩＬ－２Ｒ水平哮喘组高于支气管炎和正常组（ｐ＜０．０１），后两者差异无显著性。以上结果表明，哮喘发病中存在着Ｔ细胞的活化，ＩＬ－２／ＩＬ－２Ｒ在哮喘发病中起作用。     

他巴唑对Graves病患者血清可溶性白细胞介素—6受体的影响

５２例Ｇｒａｖｅｓ病（ＧＤ）患者随机分两组,２６例接受他巴唑（ＭＭＩ）、另２６例接受ＭＭＩ加心得安治疗。应用酶联免疫吸附分析（ＥＬＩＳＡ）,测定了未治疗期（第０周）,部分缓解期（第２周）和缓解期（第６周）患者及２０名健康对照者血清可溶性白细胞介素－６受体（ｓＩＬ－６Ｒ）水平。     

High Level of Serum Soluble Interleukin-2 Receptor at Transplantation Predicts Poor Outcome of Allogeneic Stem Cell Transplantation for Adult T Cell Leukemia

The prognosis for adult T cell leukemia/lymphoma (ATL) is very poor, and only allogeneic hematopoietic stem cell transplantation (allo-SCT) has been considered to be a curative treatment for ATL. In this study, we retrospectively analyzed data for patients who had received allo-SCT for ATL in Hokkaido, the northernmost island of Japan, to determine prognostic factors. Fifty-six patients with a median age of 57 years received allo-SCT. Twenty-eight (50.0%) patients had acute type and 22 (46.4%) had lymphoma type. Twenty-three (41.1%) patients received allo-SCT in complete remission (CR), whereas the others were in non-CR. Seventeen (30.4%) patients received myeloablative conditioning and the others received reduced-intensity conditioning. With a median follow-up period of 48 months (range, 17 to 134 months), 1-year overall survival (OS) and 5-year OS rates were 55.4% and 46.1%, respectively. The survival curve reached a plateau at 22 months after stem cell transplantation (SCT). Male sex, high level of serum soluble interleukin-2 receptor (sIL-2R) at SCT, and non-CR at SCT were determined to be significant risk factors for OS. A high level of sIL-2R at SCT was a risk factor for poor OS in patients with non-CR at SCT by univariate analysis (P = .02), and it remained significant after adjustment by sex (hazard ratio, 2.73 [95% confidence interval, 1.07 to 7.90]). A high level of sIL-2R at SCT was also determined to be a risk factor for disease progression (P = .02). This region-wide study showed encouraging results for survival after allo-SCT for ATL and demonstrated for the first time that a high level of sIL-2R at SCT predicts worse SCT outcome.     

Sexual disparities in the incidence and course of SLE and RA

Systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) disproportionately affect females compared to males, with female to male prevalence ratios of 7–9:1 for SLE and 2–3:1 for RA. Interestingly, epidemiologic studies indicate that men that develop SLE may have more morbidity than women, but the same is not true for RA. Given the sex and age bias of SLE and RA, sex hormones may influence the pathogenesis of these diseases. However, the ways in which, and to what degree, sex hormones affect disease incidence and severity remain unclear and is the topic of ongoing research. Recent findings have implicated interactions between sex hormones, the immune system, genetic factors, and epigenetic modifications in influencing SLE and RA disease activity. This article reviews current hypotheses regarding the potential impact of sex hormones and genetics on disease pathogenesis, incidence, and severity of SLE and RA.