高效液相色谱法测定赤芍中芍药苷的含量

目的建立赤芍中芍药苷的含量测定方法 ,探讨不同溶液提取对芍药苷含量的影响。方法采用甲醇和70%乙醇超声提取样品,用Agileni1100型高效液相色谱仪,Agilent Eelipse XDBC18（4.6mm×250mm,5μm）色谱柱,柱温：25℃,流动相：V（乙腈）：V（0.1%磷酸溶液）=14：86,流速：1.0ml/min,检测波长：230nm,进样体积10μL。结果芍药苷进样量在0.27μg～1.60μg范围内与峰面积呈良好线性关系,R=0.9998;平均回收率为100.34%,RSD=0.46%。乙醇提取液中芍药苷的平均含量为0.0700μg/ml（n=3）,甲醇提取液中芍药苷的平均含量为0.0462μg/ml（n=3）。结论采用70%乙醇作为溶液提取赤芍芍药苷得率高;本实验所建立的方法重复性良好,准确度较高,可用于赤芍中芍药苷的质量控制。     

杜仲纯中药制剂和松脂醇二葡萄糖苷对自发性高血压大鼠肾脏微循环血流灌注及其频谱特征的影响

目的:探讨杜仲纯胶囊(EU)和松脂醇二葡萄糖苷(PDG)对自发性高血压大鼠(SHRs)肾脏微循环血流灌注及其频谱特征的影响。方法:将30只SHRs随机分为SHR组,SHR+EU组(6.00g/kg EU灌胃),SHR+PDG (L)组(8.75mg/kg PDG灌胃),SHR+PDG (M)组(17.50mg/kg PDG灌胃)和SHR+PDG (H)组(35.00mg/kg PDG灌胃),每组各6只。6只WKYs大鼠为Control组。各组进行灌胃给药2个月。手术暴露大鼠肾脏,利用激光多普勒血流探测仪和血流成像仪检测并比较各组大鼠肾脏微血流灌注水平和血流成像情况。采用小波变换比较各组肾脏内皮源性频段幅值。结果:与Control组相比,SHR组肾脏血流灌注水平显著降低(P<0.01)。治疗2个月后,与SHR组相比,SHR+EU组和SHR+PDG各剂量组肾脏血流灌注水平显著升高(P均<0.01)。与Control组相比,SHR组肾脏微血流灌注内皮细胞源频段振幅显著降低(P<0.01)。与SHR组比较,SHR+PDG(M)组、SHR+PDG(H)组中NO依赖和非依赖性内皮...     

高效液相色谱法测定赤芍中芍药苷的含量

目的 建立赤芍中芍药苷的含量测定方法,探讨不同溶液提取对芍药苷含量的影响.方法 采用甲醇和70%乙醇超声提取样品,用Agileni1100型高效液相色谱仪,Agilent EeliPse XDB C18(4.6mm×250mm,5靘)色谱柱,柱温:25℃,流动相:V(乙腈):V(0.1%磷酸溶液)=14:86,流速:1.0ml/min,检测波长:230nm,进样体积10μL.结果 芍药苷进样量在0.27μg～1.60μg范围内与峰面积呈良好线性关系,R=0.9998;平均回收率为100.34%,RSD=0.46%.乙醇提取液中芍药苷的平均含量为0.0700μg/ml(n=3),甲醇提取液中芍药苷的平均含量为0.0462μg/ml(n=3).结论 采用70%乙醇作为溶液提取赤芍芍药苷得率高;本实验所建立的方法重复性良好,准确度较高,可用于赤芍中芍药苷的质量控制.     

基于Nrf2通路探讨松脂醇二葡萄糖苷改善小鼠骨质疏松的机制研究

目的 分析松脂醇二葡萄糖苷（Pinoresinol diglucoside,PD）改善骨质疏松的作用机制。方法 微型CT分析小鼠小梁骨体积/总体积（bone volume/total volume,BV/TV）、平均小梁厚度（average trabecular thickness,Tb.Th）、平均小梁数目（average number of trabeculae,Tb.N）和平均小梁间距（average trabecular spacing,Tb.Sp）;HE染色检测骨组织损伤;TRAP染色分析成骨细胞数量/骨周长（number of bone cells/bone circumference,N.Ob/B.Pm）、空泡率以及破骨细胞表面/骨表面百分比（osteoclast surface/bone surface,Ocs/BS）;ELISA检测碱性磷酸酶（alkaline phosphatase,ALP）、抗酒石酸酸性磷酸酶（tartrateresistant acid phosphatase,TRAP）相对活性,超氧化物歧化酶（Superoxide dismutase,SOD）...     

HPLC法测定豆腐渣果中豆腐果苷的含量

<正>豆腐渣果为山龙眼科山龙眼属(Helicia)植物深绿山龙眼(Helicia nilagirica Bedd)的种子,其有效成分豆腐果苷(Helicid,对苯甲醛-O-β-D-阿洛吡喃糖苷)的化学结构类似于天麻苷(Gastrodin,对羟甲基苯-O-β-D-吡喃葡萄糖苷)[1]。药理研究表明,豆腐果苷对中枢神经系统的作用与     

HPLC法测定化淤生骨胶囊中人参皂苷Rg1和 人参皂苷Rb1的含量

目的 建立化淤生骨胶囊中人参皂苷Rg1和人参皂苷Rb1的含量测定方法。方法 采用高效液相色谱法（HPLC）测定。色谱柱为Kromasil-C18(5 μm、4.6 mm×250 mm),乙腈-水为流动相进行梯度洗脱,检测波长203 nm。结果 人参皂苷Rg1浓度在0.0522 mg/ml～0.8220 mg/ml范围内与峰面积的线性关系良好（γ=0.9994）; 人参皂苷Rb1浓度在0.0583 mg/ml～07830 mg/ml范围内与峰面积的线性关系良好（γ=0.9990）。结论 所建方法准确,重复性好,可用于测定化淤生骨胶囊中的人参皂苷Rg1和人参皂苷Rb1。     

HPLC法测定对乙酰氨基酚片的含量

目的 建立HPLC法测定对乙酰氨基酚片的含量。方法 色谱柱为Agilent Eclipse XDB-C18柱（4.6 mm×150 mm,5 μm）;流动相为甲醇-水（20∶80）,流速为1.0 ml/min,检测波长为244 nm,柱温30 ℃。结果 对乙酰氨基酚浓度在10.16~30.48 μg/ml范围内线性关系良好,r=0.9998（n=5）;平均加样回收率为100.04%,RSD=0.95%（n=6）。结论 HPLC法操作简便、结果准确、重复性好、专属性强,适用于测定对乙酰氨基酚片中对乙酰氨基酚的含量。     

高效液相色谱法测定芦荟排毒胶囊中芦荟大黄素的含量

目的:建立高效液相色谱法测定芦荟排毒胶囊中芦荟大黄素的方法。方法:采用W aters 2690型高效液相色谱仪(美);检测器:996 PDA二极管阵列检测器;色谱柱:D iamonsil C18柱(200mm×4.6mm,5μm);流动相:甲醇-水(80:20);流速:1m l/m in;于254nm波长处以外标法测定。结果:在0.0748-7.48 g/m l范围内浓度与峰面积有良好的线性关系(r=0.9999)。平均回收率为99.2%,RSD为3.18%。结论:方法简便可靠,可用于测定芦荟排毒胶囊中芦荟大黄素的含量。     

高效液相色谱法测定血清碘海醇浓度方法的建立和应用

目的 建立利用高效液相色谱法(HPLC)测定血清碘海醇浓度的方法,为碘海醇血浆清除率临床应用及推广提供方法学支持.方法 设定色谱条件,对HPLC测定血清碘海醇浓度的方法进行方法学验证.对33例慢性肾脏病(CKD)儿童同时进行碘海醇清除率与99mTc-DTPA血浆清除率测定,并采用最新Schwartz公式估算肾小球滤过率(eGFR).以99mTc-DTPA血浆清除率为参照,分析碘海醇清除率及eGFR与参照方法的相关性和一致性.结果 在设定HPLC色谱条件下,碘海醇浓度在10～1 000 mg/L范围内线性良好(R2 =0.9998).批间测量和批内测量相对标准偏差(RSD％)＜5％,平均回收率(R％)＞96％.碘海醇血浆清除率、eGFR与99mTc-DTPA血浆清除率的相关系数(r)分别为0.95和0.63,偏差分别为(6.24±10.81)mL/(min·1.73 m2)和(12.11±22.96) mL/(min·1.73 m2).结论 建立了测定血清碘海醇浓度的HPLC法;初步探索并验证了碘海醇血浆清除率评价儿童肾功能的准确、可行.     

The Prevalence of Severe Pain,its Etiopathological Characteristics and Treatment Profile of Patients Referred to A Tertiary Cancer Care Pain Clinic

Pain is the most feared symptom in cancer. About 52–77% patients suffer pain despite World Health Organization (WHO) recommendations. Out of total, one-third patients suffer moderate to severe pain. This study was undertaken to determine the prevalence, etiopathogenesis and characteristics of severe pain and treatment response among pain clinic referrals in a busy tertiary care cancer center. This study found a high prevalence (31.5%) of severe pain. A total of 251 patients who had complete pain data were analyzed for etiopathological characteristics and treatment response. Head and neck cancer contributed the highest prevalence among all regions. Oncologists prescribed non-steroidal anti-inflammatory drugs (NSAIDs) or paracetamol with or without mild opioids to 14% patients and pain clinic physicians prescribed opioids and overall 63.7% patients had a better response after pain clinic referral, even then, morphine was not prescribed to many deserving patients. Doctors need pain education about opioids to remove any fear of prescribing opioids in presence of severe pain.     

Breakthrough Cancer Pain: Review of Prevalence, Characteristics and Management

Breakthrough pain has been associated with a reduced likelihood of adequate pain control. Despite the large and variable incidence of this phenomenon due to varied definitions of this type of pain, only a few studies have been conducted to assess and effectively treat breakthrough pain though the importance of managing breakthrough pain has been acknowledged by all. A large number of drugs from various classes and novel methods of administration like nasal and transmucosal buccal route, as in the case of fentanyl, have been used in these studies to manage this type of pain. A drug is needed with a quick onset of action and optimal duration that matches the characteristics of breakthrough pain. Some steps have been taken in earlier studies which used nasal formulation of fentanyl as it was found to achieve adequate and quick pain relief. However, further studies are required to confirm this so that in the future we can have as effective protocols for managing breakthrough pain as we have today for managing persistent pain as given by the World Health Organization.     

以HPLC法测定马来酸氯苯那敏含量的方法中马来酸峰及氯苯那敏峰定位的问题

目的：探讨在《国家药品标准化学药品地方标准上升国家标准》收载品种中采用HPLC方法测定马来酸氯苯那敏的含量时,所采集的HPLC图谱中,出现的马来酸峰与氯苯那敏峰保留位置如何确定的问题。方法：采用高效液相色谱法,色谱柱：Waters C18（4．6mm×150mm,5μm）,江苏淮阴C18（4．6mm×250mm,5μm）;流动相：庚烷磺酸钠溶液（冰醋酸调pH值至3．3）-甲醇-乙腈（25：30：18）;检测波长262nm。结果：在本文收集的试验条件下所采集的色谱图中,马来酸氯苯那敏对照品均出现了前后两个峰,马来酸峰与氯苯那敏峰保留位置可能在不同的色谱条件下有明显变化。结论：色谱图中马来酸峰与氯苯那敏峰保留位置应加以确定,以防止对马来酸氯苯那敏对照品纯度产生疑虑。     

HPLC法同时测定益心舒片中丹参酮ⅡA的含量

目的：建立采用 HPLC法测定益心舒片中丹参酮ⅡA含量的方法。方法采用 KromasilC18色谱柱(4.6 mm×250 mm,5 um),以甲醇原水(82：18)水梯度洗脱,流速为1 mL/min,柱温30℃;紫外检测器波长为270 nm。结果丹参酮ⅡA在2.556~51.12μg/ml范围内呈良好的线性关系(r=0.9998)。其平均回收率为97.58%,RSD为0.6%(n=9)。结论本方法灵敏高、简便、重复性好,可用于益心舒片中丹参酮ⅡA的含量测定。     

HPLC法测定卤米松乳膏中有关物质

目的 建立HPLC法检测卤米松乳膏中的有关物质.方法 采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm);以0.03％ 冰醋酸为流动相A,乙腈为流动相B,按一定程序进行梯度洗脱;流速为0.8 ml/min;检测波长为238 nm;柱温为35℃;进样体积为20μl.结果 该法专属性...     

Complementary and Alternative Medicine in Cancer Pain Management: A Systematic Review

Quality of life (QoL) encompasses the physical, psychosocial, social and spiritual dimensions of life lived by a person. Cancer pain is one of the physical component has tremendous impact on the QoL of the patient. Cancer pain is multifaceted and complex to understand and managing cancer pain involves a tool box full of pharmacological and non pharmacological interventions but still there are 50-70% of cancer patients who suffer from uncontrolled pain and they fear pain more than death. Aggressive surgeries, radiotherapy and chemotherapy focus more on prolonging the survival of the patient failing to realize that the QoL lived also matters equally. This paper reviews complementary and alternative therapy approaches for cancer pain and its impact in improving the QoL of cancer patients.