肌醇硫酸酯铝对胃溃疡大鼠黏膜保护作用的研究

目的：研究肌醇硫酸酯铝对大鼠胃黏膜损伤的保护作用。方法：采用幽门结扎急性胃溃疡、乙酸烧灼慢性胃溃疡模型观察肌醇硫酸酯铝对胃溃疡大鼠黏膜的保护作用,测定胃溃疡指数和溃疡抑制百分率。同时幽门结扎形成急性胃溃疡后测定胃液量、胃蛋白酶活性、pH值、游离盐酸和总酸度。结果：在急性胃溃疡模型中,与空白组相比,肌醇硫酸酯铝可降低胃溃疡指数,提高溃疡抑制率（P〈0.05）,奥美拉唑及多司马酯组亦有同样作用;但在抗乙酸诱发的慢性溃疡实验中,肌醇硫酸酯铝可有效抑制胃溃疡的生成,降低胃溃疡指数,提高溃疡抑制率（P〈0.05）;而多司马酯组及奥美拉唑组无显著影响。与空白组相比,肌醇硫酸酯铝中、低剂量及多司马酯组对pH值和总酸度影响不明显;而肌醇硫酸酯铝高剂量组可以有效抑制胃酸产生,抑制胃蛋白酶活性（P〈0.05）。结论：肌醇硫酸酯铝可抑制溃疡的发生,降低胃溃疡指数,并具有剂量依赖性。     

张氏益胃汤对乙酸性胃溃疡大鼠生长因子的影响

目的：观察张氏益胃汤对胃溃疡大鼠生长因子VEGF、bFGF、EGF及TGF-a表达的影响,探讨张氏益胃汤治疗胃溃疡的作用机制。方法：将50只SD大鼠随机分为正常对照组、溃疡模型组、中药小剂量组、中药大剂量组、西药治疗组共5组,每组10只。采用改良Okbae乙酸涂抹法复制实验性胃溃疡模型,中药治疗组给予张氏益胃汤灌胃,西药治疗组给予雷尼替丁灌胃,共14d;实验后ELISA检测各组血清VEGF和bFGF以及免疫组化检测胃黏膜组织EGF和TGF-a含量的变化。结果：中药治疗组和西药治疗组均能较正常对照组明显增加大鼠胃溃疡血清VEGF、bFGF和胃组织EGF、TGF-a水平,差异具有统计学意义（P〈0.05,P〈0.01）。结论：张氏益胃汤通过促进细胞生长因子VEGF、bFGF、EGF、TGF-a的表达,从而发挥抗溃疡作用。     

番茄红素对实验性肺纤维化大鼠血浆TNF-α、NO、MDA含量及SOD活性的影响

目的　考察番茄红素对实验性肺纤维化大鼠血浆 TNF-α、NO、MDA含量及 SOD活性的影响。方法　 6 0只 SD大鼠随机分为正常对照组 (C组 )、模型组 (M组 )和番茄红素干预组 (L组 ) ,每组各 2 0只。 M组和 L组采用博莱霉素 (BL M)经气管注射建立大鼠肺纤维化模型 ,造模当日起 L组每天用番茄红素油树脂灌胃 ,3、7、14和 2 8d处死大鼠采集血浆 ,测定 TNF-α、NO、MDA含量及 SOD活性。结果　与 M组相比 ,L组各时间段 TNF-α、NO、MDA含量降低 (7、14、2 8d,TNF-α:P<0 .0 1;3d,NO、MDA:P<0 .0 5 ) ,SOD活性增高 (2 8d,P<0 .0 5 )。结论　番茄红素能够降低实验性肺纤维化大鼠血浆 TNF-α、NO、MDA含量 ,提高 SOD活性 ,对博莱霉素致大鼠的肺损伤具有一定保护作用     

活血化瘀药对乙酸致胃溃疡大鼠表皮生长因子及其受体表达的影响

目的：观察活血化瘀药对乙酸致胃溃疡大鼠胃组织及血清表皮生长因子（EGF）和胃组织表皮生长因子受体（EGFR）表达的影响,探讨活血化瘀药促进胃溃疡愈合的机制。方法：采用实验性乙酸致大鼠胃溃疡模型,随机分为空白组、模型组、活血化瘀组、雷尼替丁组,连续给药治疗14天,采用R IA法测定血清及胃组织中EGF的含量,采用免疫组织化学SP法检测胃组织中EGFR的表达。结果：与模型组相比,雷尼替丁组、活血化瘀组胃组织EGF含量明显增高（P〈0.01）,血清EGF含量明显增高（P〈0.05）,雷尼替丁组、活血化瘀组EGFR的面积百分比明显增高（P〈0.05）,活血化瘀组EGFR的积分光密度明显增高（P〈0.05）。结论：活血化瘀药能增高胃组织和血清中的EGF及EGFR的表达,其EGF通过与其受体EGFR结合而起到促进溃疡愈合的作用。     

溃疡平对胃溃疡大鼠胃黏膜组织中EGF和EGFR mRNA表达的影响

目的观察溃疡平对胃溃疡大鼠胃黏膜组织表皮生长因子(EGF)和表皮生长因子受体(EG-FR)mRNA表达的影响,探讨溃疡平的促进胃溃疡愈合的机制。方法采用改良Okabe法制备慢性乙酸胃溃疡模型;随机分为:实验对照组,模型组,溃疡平高、中、低剂量组,盐酸雷尼替丁组,共6组;给药治疗14 d,采用实时荧光定量PCR法检测胃黏膜组织EGF和EGFR mRNA表达。结果与模型组相比,溃疡平低、中、高剂量组的EGF 2-ΔΔCT值明显增高,表明溃疡平能使EGF mRNA表达上调,有统计学意义(P<0.01);溃疡平低、中、高剂量组的EGFR 2-ΔΔCT值明显增高,表明溃疡平能使EGFR mRNA表达上调,有统计学意义(P<0.001)。结论溃疡平可促进胃溃疡大鼠胃黏膜组织EGF和EGFR mRNA表达,这可能是其促进溃疡愈合,提高溃疡愈合质量,减少溃疡复发的分子学基础之一。     

水飞蓟喷雾剂对大鼠深Ⅱ度烫伤创面修复的影响

目的考察水飞蓟喷雾剂对大鼠深Ⅱ度烫伤创面修复作用及机制。方法建立深Ⅱ度烫伤模型大鼠,随机分为模型组、美宝湿润烧伤膏组(24 g/kg)、水飞蓟喷雾剂小剂量组(24 g/kg)、水飞蓟喷雾剂中剂量组(48 g/kg)、水飞蓟喷雾剂大剂量组(96 g/kg)和空白组,连续给药21 d。分别在造模后第3、7、14、21天计算烫伤创面愈合率,检测血清超氧化物歧化酶(superoxide dismutase,SOD)活性和丙二醛(malondialdehyde, MDA)含量;免疫组化法检测烫伤创面碱性成纤维细胞生长因子(basic fibroblast growth factor,b-FGF)、表皮生长因子(epidermal growth factor, EGF)、转化生长因子-β(transformating growth factor betal, TGF-β)的表达。结果与模型组相比,烫伤后第3天,水飞蓟喷雾剂小剂量组、中剂量组、大剂量组创面愈合率提高,血清SOD活性升高,MDA含量降低,且差异均具有统计学意义(P0.05);烫伤后第7、14天,水飞蓟喷雾剂大剂量组血清SOD活性升高,MDA含量降低,差异均具有统计学意义(P0.05);同时水飞蓟喷雾剂各剂量组均可不同程度诱导受损组织内bFGF、EGF、TGF-β的表达。结论水飞蓟喷雾剂对大鼠深Ⅱ度烫伤创面有修复作用,可促进烫伤创面的愈合,其机制可能与抗氧化,增加b-FGF、EGF、TGF-β的表达有关。     

螺旋藻多糖对大鼠实验性胃溃疡作用的影响

目的观察螺旋藻多糖（PSP）对乙酸烧灼型胃溃疡大鼠血清中活性成分的影响,初步探讨其治疗胃溃疡的可能机制。方法采用乙酸烧灼法制备实验性胃溃疡大鼠模型,将50只Wistar大鼠随机分成正常组、模型组、奥美拉唑组和PSP高、低剂量（400、200 mg/kg）组,每组各10只,连续药物治疗15 d后取血,分别采用硝酸还原酶法、黄嘌呤氧化酶法及硫代巴比妥酸法测定大鼠血清中一氧化氮（NO）、超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。结果 PSP高低剂量组与奥美拉唑组均能升高血清NO含量[（26.71±6.47）、（32.60±4.65）μmol/mL],提高血清SOD活力[（292.74±52.44）、（303.57±13.80）U/mL],降低MDA含量[（15.60±2.39）、（15.57±2.19）nmol/mL],与模型组比较差异有统计学意义（P〈0.05或P〈0.01）。结论 PSP具有一定的抗胃溃疡的作用,其机制可能与提高血清NO含量和SOD活性、降低MDA含量有关。     

羧甲基壳聚糖对大鼠胃溃疡抗氧化作用研究

目的:探讨羧甲基壳聚糖对大鼠乙酸型胃溃疡的保护作用及可能机制。方法:将40只SD大鼠随机分为正常组、模型组、硫糖铝组和羧甲基壳聚糖组。用20%乙酸0.05ml浆膜下注射法建立大鼠慢性胃溃疡模型,手术后第3天开始灌胃给药,连续14天后计算各组溃疡面积,同时检测胃粘膜组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:羧甲基壳聚糖、硫糖铝治疗后,乙酸诱导的溃疡面积缩小;羧甲基壳聚糖能提高大鼠胃粘膜SOD活性,抑制MDA的升高,其作用与硫糖铝相当。结论:羧甲基壳聚糖对大鼠乙酸型胃溃疡有一定保护作用,可能与它能提高胃粘膜抗氧化能力有关。     

清热解毒方对实验性胃溃疡大鼠模型的作用及对胃黏膜中表皮生长因子表达的调节机制

目的探究清热解毒方对胃溃疡大鼠模型的保护作用及其对胃黏膜中表皮生长因子表达的调节。方法选择50只SD雄性大鼠,用乙酸复制实验性胃溃疡大鼠模型,随机分为对照组、模型组、兰索拉唑组、清热解毒方组和联合给药组,对照组和模型组灌胃蒸馏水,兰索拉唑组灌胃兰索拉唑,清热解毒方灌胃清热解毒方,联合给药组灌胃兰索拉唑和清热解毒方。比较各组胃溃疡指数、胃溃疡抑制率、胃液pH值、胃蛋白酶活性、胃组织病理变化、血清中EGF和EGFR水平。结果清热解毒方组和兰索拉唑组胃溃疡指数和胃蛋白酶活性均显著大于联合给药组,差异有统计学意义(P<0.05);清热解毒方组和兰索拉唑组大鼠的胃溃疡抑制率、胃液pH值、血清中EGF和EGFR水平均显著小于联合给药组,差异有统计学意义(P<0.05);清热解毒方组和兰索拉唑组胃溃疡指数、胃蛋白酶活性、胃溃疡抑制率、胃液pH值、血清中EGF和EGFR水平均没有显著性差异(P>0.05);兰索拉唑组和清热解毒方组大鼠胃黏膜损伤程度显著降低,胃黏膜上皮变性坏死以及溃疡症状均小于模型组,胃黏膜上水肿、出血、炎症等症状轻中度;联合给药组大鼠胃黏膜损伤显著改善,与对照组大鼠胃组织病理变化基本相同。结论清热解毒方可以有效改善乙酸致实验性胃溃疡大鼠的胃溃疡症状,但联合应用西药与清热解毒方时,则能收到更加有效的治疗效果。清热解毒方促进胃溃疡修复过程的机制可能为促进EGF和EGFR表达。     

参麦对大鼠失血性休克早期炎症反应的抑制作用研究

目的探讨参麦对大鼠失血性休克后早期炎症反应的抑制作用。方法将30只Wistar大鼠随机分为3组,每组10只,分别为对照组(A组)、失血性休克组(B组)和失血性休克参麦治疗组(C组)。采用ELISA法测定血清白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)含量;采用化学反应法测定过氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果 B组血清IL-1β、IL-6、TNF-α及MDA含量均显著高于A组(P<0.01),SOD活性明显低于A组(P<0.01)。而C组与B组比较,血清IL-1β、IL-6、TNF-α及MDA含量显著降低(P<0.05),SOD活性显著升高(P<0.05)。结论参麦能有效抑制大鼠失血性休克后早期炎症因子的表达,增强SOD的活性,从而抑制大鼠休克后早期炎症反应。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.