大鼠局部创伤一周内组织中TNF、MDA和SOD变化

我们测定了大鼠局部皮肤切除后创面自然愈合过程中,肉芽组织内肿瘤坏死因子(TNF)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量的动态变化,以探讨它们在创伤修复中的变化或相互关系。结果表明:伤后3、5、7天,TNF、SOD 呈Ⅴ型改变,TNF 含量第7天与第5天、SOD 含量第7天与第3天有显著差异(P<0.05),MDA 含量逐渐升高,第7天与第3天比较有显著差异(P<0.05)。相关性检验表明:TNF 与 MDA 各时间点均呈显著正相关。TNF 与 SOD 在伤后3、7天呈显著正相关。TNF 含量<90ng/g 蛋白时创面愈合最佳,SOD 低浓度时创面愈合延缓。提示:在局部创伤修复过程中存在 TNF、MDA、SOD 的变化;内源性低浓度 TNF 及抗氧化能力的提高,对创面修复是有益的。     

大鼠局部创伤一周内组织中TNF,MDA和SOD变化

我们测定了大鼠局部皮肤切除后创面自然愈合过程中，肉芽组织内肿瘤坏死因子（ＴＮＦ）、超氧化物歧化酶（ＳＯＤ）、丙二醛（ＭＤＡ）含量的动态变化，以探讨它们在创伤修复中的变化或相互关系。结果表明：伤后３、５、７天，ＴＮＦ，ＳＯＤ呈Ｖ型改变，ＴＮＦ含量第７天与第５天、ＳＯＤ含量第７天与第３天有显著差异，ＭＤＡ含量逐渐升高，第７天与第２天比较有显著差异。相关性检验表明：ＴＮＦ与ＭＤＡ各时间点均呈显著正相关。     

不同修复阶段创面肉芽组织中肿瘤坏死因子含量的变化

采用大鼠背部刀伤模型与酶联免疫检测技术，动态观察伤后３、５、７天创面肉芽组织中内源性肿瘤坏死因子α（ＴＮＦα）含量的变化，并探讨这种变化与组织修复的关系，结果表明，伤后３个时间点创面肉芽组织中都检测出一定量的ＴＮＦα，且ＴＮＦα含量越低，创面面积越小，肉芽中蛋白含量越高，修复效果越好，结果提示创伤修复需要一定量ＴＮＦα的刺激，创面低浓度的ＴＮＦα可能较高浓度的ＴＮＦα更利于创伤修复。     

1,6—二磷酸果糖对内毒素休克犬TNF,MDA,SOD的影响

目的：观察１，６－二磷酸果糖对内毒素休克犬血浆ＴＮＦ，ＭＤＡ水平和ＲＢＣ－ＳＯＤ活性的影响，为ＦＤＰ治疗ＥＴ休克提供实验依据。方法：１２只犬随机分成两组，内毒素休克组和内毒素休克１，６－二磷酸果糖治疗组，每组６例，外周静脉注射灭活大肠杆菌３０分钟、９０分钟后、ＦＤＰ组输入７．５％ＦＤＰ３７５ｍｇ／ｋｇ，ＥＴ组输入等容量平衡盐液。注ＥＴ前及注后２、４、６、８小时测ＣＯ及血浆ＴＮＦ、ＭＤＡ水平和ＲＢＣ     

针刺对光老化大鼠皮肤组织中SOD、MDA、GSH-Px活性的影响

目的:研究针刺对UV照射引起的皮肤光老化大鼠皮肤组织中超氧化物歧化酶(SOD)、丙二醛(MDA)的含量及谷胱甘肽氧化酶(GSH-Px)活性的影响,探讨针刺对抗皮肤光老化的作用。方法:将大鼠随机分成4组,除正常组外,其余各组模拟日光中UV(UVA+UVB)照射,造成皮肤光老化模型。每次造模前,针刺组给予电针刺激,阳性对照组采用VE涂抹造模部位,15周后对比各组生化指标结果。结果:模型组与正常组比较大鼠皮肤组织中SOD、GSH-Px活性明显降低,MDA含量明显增加(P<0.05);针刺组与模型组比较SOD、GSH-Px活性增加,MDA含量明显降低(P<0.05),与VE组比较无统计学意义(P>0.05)。结论:针刺可对抗UV引起的皮肤光老化,其机理可能是通过增强皮肤中SOD、GSH-Px活性,降低MDA含量来改变皮肤的老化外观的。     

补肾健脾中药方对去卵巢大鼠血清SOD和MDA的影响

目的探讨补肾健脾中药方对去卵巢大鼠血清SOD和MDA的影响。方法 48只6月龄的雌性SD大鼠随机分为正常空白组、模型组、中药组、西药组。治疗12周后,收集左侧股骨、血清分别行BMD测量和SOD、MDA检测。结果最后纳入研究动物40只。股骨BMD值:模型组低于正常空白组(P0.01);观察组、对照组分别与模型组比较,差异有统计学意义(P0.05)。在SOD方面,观察组、对照组的SOD值均比模型组高,三者比较,有统计学意义(P0.05)。在MDA方面,观察组、对照组的MDA值均比模型组低,三者比较,有统计学意义(P0.05)。结论补肾健脾中药康方具有提高去卵巢大鼠BMD的作用;能够提高血清中SOD的含量,同时减低MDA含量,在一定的程度上清理自由基,达到防治骨质疏松症的目的。     

硬膜内局部灌注甲强龙对兔急性脊髓损伤后血清SOD、MDA的影响

目的探讨硬膜内局部灌注甲强龙(MP)对兔脊髓损伤(SCI)后血清超氧化物歧化酶(SOD)、丙二醛(MDA)的浓度的影响。方法将54只白兔随机分为3组,每组18只,Ⅰ组为不完全SCI+局部灌注MP,Ⅱ组为不完全SCI+局部灌注生理盐水(NS),Ⅲ组为对照组。用改良Allen法造成Ⅰ、Ⅱ组不完全SCI模型,硬膜内置管,检测血清中SOD、MDA的变化情况,同时进行改良Tarlov评分测评及病理组织学观察。结果Ⅱ组血清中的MDA升高,SOD浓度下降,与Ⅲ组比较差异有统计学意义(P<0.05)。与Ⅱ组比较,Ⅰ组能明显降低SCI后血清中MDA含量,提高SOD浓度,差异有统计学意义(P<0.05)。结论 SCI后硬膜内局部灌注MP能够降低血清MDA含量,提高SOD浓度,在一定程度上阻止继发性损伤的发展。     

家兔创伤休克后血浆内毒素,TNF和IL—6的动态变化

目的：探讨创伤休克对内毒素移位的影响及其与ＴＮＦ、ＩＬ－６产生的关系。方法：选用家兔１６只，随机分为创伤合并失血性休克（Ⅰ组）和单纯失血性休克组（Ⅱ组），采用鲎试验基质显色法，ＥＬＩＳＡ和细胞生物测定法分别测定血浆内毒素、ＴＮＦ和ＩＬ－６水平。结果：休克１．５小时，Ⅰ组血浆内毒素水平即明显高于伤前，至复苏后０．５小时达峰值，复苏后１小时仍明显高于伤前。休克后Ⅰ组血浆内毒素水平明显高于Ⅱ组；休克及复苏后，血浆ＴＮＦ、ＩＬ－６水平也先后显著升高，其中ＴＮＦ升高较早，Ⅰ组血浆细胞因子水平明显高于Ⅱ组；相关分析表明，创伤休克后血浆ＴＮＦ、ＩＬ－６均值分别与血浆内毒素均值呈显著正相关。结论：创伤休克可导致明显的内毒素血症及ＴＮＦ、ＩＬ－６等细胞因子过量产生，且较单纯休克时明显，创伤后细胞因子产生与内毒素移位有一定的内在联系。     

神经肽P物质与烫伤创面愈合关系的实验研究

目的　探讨神经肽P物质(SP)与烫伤创面愈合之间的关系。　方法　(1)制作大鼠不同深度烫伤模型,分别于伤后1、3、7、14d致死,用放射免疫法测定创面SP含量。(2)将大鼠肉芽组织成纤维细胞(GTF)用不同培养液培养,分为空白对照组、SP组及SP SP受体拮抗剂(Spantide)组。体外检测SP及Spantide对GTF增殖活性[以吸光度(A)值表示]及凋亡率的影响。　结果　(1)伤后1d,浅Ⅱ、深Ⅱ、Ⅲ度烫伤创面SP含量分别为(145±78)、(94±48)、(53±27)ng/g,深Ⅱ度创面与其余两者比较,差异有统计学意义(P<0 01).浅Ⅱ度创面伤后3、7dSP含量显著增高;深Ⅱ度创面伤后7、14dSP含量显著增高;Ⅲ度创面伤后SP含量无显著变化。(2)SP增强GTF增殖活性(空白对照组A为0. 21±0. 05,SP组A为0. 36±0 07,P<0. 01)并抑制其凋亡,Spantide可抑制SP对GTF的作用。　结论　SP可促进GTF增殖,创面SP含量与创面损伤程度及愈合能力关系密切。     

不同剂量rhGM-CSF凝胶促进大鼠深Ⅱ度烧伤创面愈合的实验研究

目的：观察不同剂量重组人粒细胞-巨噬细胞集落刺激因子（rhGM-CSF）凝胶（商品名为金扶宁）对SD大鼠深Ⅱ度烫伤创面愈合的促进作用并筛选最佳剂量.方法：42只SD大鼠,雌雄各半,随机分为7组：模型组,凝胶基质对照组,金扶宁100、250、500、750和1 000 mg/cm^2治疗组.每只大鼠背部制备两个深Ⅱ度烫伤创面,凝胶基质对照组创面于伤后1～10 d每日涂抹凝胶基质100 mg/cm^2,然后于伤后12、14、17和19 d间断创面涂抹凝胶基质100 mg/cm^2;不同剂量金扶宁治疗组创面涂沫相应剂量金扶宁,给药方法及时间同凝胶基质对照组;模型组不予处理.伤后3、7、10、14、17、21 d分别测量痂皮形成及脱落创面数,计算创面上皮化率、创面愈合率,伤后21 d行组织学检查.结果：与模型组和凝胶基质对照组相比,金扶宁各组对烫伤后7～10 d前后创面的融痂脱痂效果非常明显,以250～500 mg/cm^2治疗组疗效最佳（P〈0.05）.治疗后7～10 d,金扶宁各组新生上皮较模型组和凝胶基质对照组均显著增多（P〈0.05）;治疗后14 d,金扶宁500 mg/cm^2治疗组上皮化率[（55.46±6.13）%]较模型组[（48.81±4.99）%]和凝胶基质对照组[（43.17±5.65）%]仍然显著增多,并优于其他剂量治疗组（P〈0.05）.治疗后10～14 d,金扶宁250～500 mg/cm2治疗组创面愈合率较模型组、凝胶基质对照组和其他剂量治疗组显著增高（P〈0.05）;治疗后21 d,金扶宁250～1 000 mg/cm^2治疗组毛囊再生数量较模型组[（1.531±0.374）/mm2]和凝胶基质对照组[（1.423±0.346）/mm^2]显著增高,以500～750 mg/cm^2治疗组[分别为（7.145±1.153）/mm2和（6.250±0.768）/mm2]明显（P〈0.05）.结论：rhGM-CSF凝胶可以促进痂皮形成和脱落、加快上皮生长、促进毛囊再生,增加烧伤创面修复速度并改善修复质量,以500 mg/cm^2剂量治疗效果最佳.     

胎兔、孕兔、成年兔伤口愈合中肿瘤坏死因子和表皮生长因子含量分析

目的：探索胎兔伤口无瘢痕愈合的可能机理及条件。方法：采用孕22－23d的母兔及所孕胎兔皮肤伤口无瘢痕愈合的动物模型及成年兔皮肤伤口愈合模型，以放射免疫法测定其表皮生长因子（EGF）和肿瘤坏死因子（TNF）含量。结果：胎儿伤口中的EGF和TNF术后3d明显升高（P＜0．05），并维持至术后7d；孕兔及成年兔仅在术后3d升高（P＜0．05），术后7d降到接近正常水平。结论：胎兔皮肤伤口中特有的细胞因子水平是胎儿伤口无瘢痕愈合的重要因素。     

Modulation of wound contracture α‐smooth muscle actin and multispecific vitronectin receptor integrin αvβ3 in the rabbit's experimental model

The myofibroblast, a major component of granulation tissue, is a key cell during wound healing, tissue repair and connective tissue remodelling. Persistence of myofibroblasts within a fibrotic lesion leads to excessive scarring impairing function and aesthetics. Various wound‐healing cytokines can be modulated by topical application of active agents to promote optimal wound healing and improve scar quality. Thus, the myofibroblast may represent an important target for wound‐healing modulation to improve the evolution of conditions such as hypertrophic scars. The purpose of this work is to study the modulation of myofibroblasts and integrin αvβ3 in a full thickness wound performed on rabbits treated with different topical agents using: (1) saline, (2) Tegaderm occlusive dressing (3) silver sulfadiazine and (4) moist exposed burn ointment (MEBO). The reepithelialisation was 4 days faster in the MEBO group compared with the other therapies with less oedema formation, delayed contraction, less inflammatory cells and the lowest transepidermal water loss (TEWL) resulting in a soft scar. Although α‐smooth muscle actin (α‐SMA) was the highest around day 12 in the MEBO group, wound contraction and myofibroblast's activity were the least for the same period probably because of a downregulation of the integrin αvβ3. It seems that the effect of MEBO could be more pronounced on force transmission rather then on force generation. Greater insight into the pathology of scars may translate into non surgical treatments in the future and further work in myofibroblast biology will eventually result in efficient pharmacological tools, improving the evolution of healing and scar formation.     

Relationship between maceration and wound healing on diabetic foot ulcers in Indonesia: a prospective study

The aim of this study was to clarify the relationship between maceration and wound healing. A prospective longitudinal design was used in this study. The wound condition determined the type of dressings used and the dressing change frequency. A total of 62 participants with diabetic foot ulcers (70 wounds) were divided into two groups: non‐macerated (n = 52) and macerated wounds (n = 18). Each group was evaluated weekly using the Bates–Jensen Wound Assessment Tool, with follow‐ups until week 4. The Mann–Whitney U test showed that the changes in the wound area in week 1 were faster in the non‐macerated group than the macerated group (P = 0·02). The Pearson correlation analysis showed a moderate correlation between maceration and wound healing from enrolment until week 4 (P = 0·002). After week 4, the Kaplan–Meier analysis showed that the non‐macerated wounds healed significantly faster than the macerated wounds (log‐rank test = 19·378, P = 0·000). The Cox regression analysis confirmed that maceration was a significant and independent predictor of wound healing in this study (adjusted hazard ratio, 0·324; 95% CI, 0·131–0·799; P = 0·014). The results of this study demonstrated that there is a relationship between maceration and wound healing. Changes in the wound area can help predict the healing of wounds with maceration in clinical settings.     

海水浸泡对烫伤大鼠创面炎性反应与愈合的影响

目的观察海水浸泡对烫伤大鼠创面炎性反应及愈合的影响。方法将144只雄性Wistar大鼠随机分为烫伤对照组和海水浸泡组,每组72只,均造成背部10%TBSA浅Ⅱ度烫伤。海水浸泡组大鼠伤后固定四肢,立即用盛海水的方盆浸泡双前肢以下部分,持续4h；烫伤对照组大鼠则用空方盆模拟浸泡过程。于伤后0(即刻,下同)、6、12、24h采用电解质分析仪测定血清中K~+、Na~+、Cl~-的浓度。于伤前及伤后0、6、12h采用酶联免疫吸附测定法检测血清中肿瘤坏死因子(TNF)α及白细胞介索(IL)6的含量。对两组大鼠创面行大体和组织病理学观察,并记录创面愈合时间。结果海水浸泡组大鼠血清中K~+、Na~+、Cl~-的浓度大多高于烫伤对照组。伤后6h海水浸泡组大鼠血清TNF-α、IL-6含量分别为(140±22)、(160±41)ng/L,均明显高于伤前值(29±15)、(62±17)ng/L及烫伤对照组(120±12)、(124±22)ng/L(P＜0．05)。与烫伤对照组比较,海水浸泡组大鼠创面水肿及局部组织炎性反应加重,创面再上皮化和表皮各层的分化延迟；海水浸泡组创面愈合时间为(16．3±1．6)d,明显迟于烫伤对照组(14．1±1．8)d(P＜0．05)。结论大鼠烫伤后经海水浸泡,可加重创面炎性反应,使创面愈合延迟。     

Microgravity and the implications for wound healing

Wound healing is a sophisticated response ubiquitous to various traumatic stimuli leading to an anatomical/functional disruption. The aim of present article was to review the current evidence regarding the effects of microgravity on wound healing dynamics. Modulation of haemostatic phase because of alteration of platelet quantity and function seems probable. Furthermore, production of growth factors that are released from activated platelets and infiltration/function of inflammatory cells seem to be impaired by microgravity. Proliferation of damaged structures is dependent on orchestrated function of various growth factors, for example transforming growth factors, platelet-derived growth factor and epidermal growth factor, all of which are affected by microgravitational status. Moreover, gravity-induced alterations of gap junction, neural inputs, and cell populations have been reported. It may be concluded that different cellular and extracellular element involved in the healing response are modified through effect of microgravity which may lead to impairment in healing dynamics.     

Relationship between expression of Bax and Bcl-2 proteins and apoptosis in radiation compound wound healing of rats

Objective: To study the relationship between the expression of Bax, Bcl-2 proteins, and apoptosis in radiation compound wound healing of rats. Methods : Apoptosis, Bax and Bcl-2 proteins were estimated by in situ terminal labeling (TUNEL) and immunohistochemical methods. Results: (1) Changes of the apoptosis in wound healing showed three typical characteristics: early occurrence, high frequency and delayed disappearance after radiation to rats when compared with those of simple wound group, which might be an important reason for radiation-induced delayed wound healing. ( 2 ) The expression of Bax protein increased evidently with the increment of apoptosis and showed a good corresponding relationship with the apoptotic frequency in the process of wound healing. While the expression of Bd-2 protein decreased obviously as the apoptosis reached a maximum and showed increasing tendency up to normal level whenthe apoptosis decreased distinctively. Conclusions： Bax and Bcl-2 proteins play an important role in the apoptotic regulation of radiation compound wound healing in rats.     

Comparison of the effects of gabapentin and pregabalin on wound healing in rats

Gabapentinoids are effective adjunct drugs for reducing postoperative pain. However, the effects of gabapentinoids on wound healing have not been evaluated yet. In this study we evaluated their effects on wound healing. A total of 17 male Wistar‐Albino rats, 250–350 g, were divided into three groups randomly: control group (n = 5, 2 ml saline), gabapentin group (n = 6, 20 mg/kg gabapentin) and pregabalin group (n = 6, 20 mg/kg pregabalin). Until day 13 inflammation scores were significantly lower (P < 0·05) and wound healing was significantly better in the control group when compared with gabapentin and pregabalin groups (P < 0·001). Inflammation scores were significantly lower in pregabalin group when compared with gabapentin group until day 13. But wound healing was significantly better in gabapentin group than in pregabalin group between days 13 and 21. In conclusion when gabapentin and pregabalin were compared, although pregabalin decreases inflammation scores, gabapentin has better results in wound healing.