Extended in vitro maturation of immature oocytes from stimulated cycles: an analysis of fertilization potential,embryo development,and reproductive outcomes

Purpose  

To investigate 24 h in vitro maturation (IVM) of cumulus-stripped immature oocytes from stimulated cycles.     

Meta-analysis of the embryo freezing transfer interval

Background

The decision of whether frozen embryo transfer (FET) should be performed in the cycle immediately after OPU or at least one cycle later is controversial. FET could improve pregnancy rates in IVF; however, how much time is needed for the endometrium to return to optimal receptivity after ovarian stimulation is not known.

Methods

Electronic search in MEDLINE, EMBASE, and Cochrane Central Register of Controlled Trials to identify studies providing data on the influence of the interval between embryo freezing (or OPU) and FET in FET cycles published between January 1, 2007, and February 1, 2020.

Main findings

Data analyzed indicated that in the immediate FET cycles, there was a trend to an increased biochemical pregnancy rate (RR = 1.08; CI = 1.00-1.18), whereas the clinical pregnancy rate was somewhat higher, but without reaching statistical significance (RR = 1.07; CI = 0.99-1.15). The live birth rate was similar in the two groups (RR = 1.05; CI = 0.95-1.15), as was the implantation rate (RR = 0.98; CI = 0.83-1.16). Stratifying by embryo stage or FET type (freeze-all or FET after failed fresh transfer) showed no differences.

Conclusion

Systematically delaying FET does not offer benefits to IVF outcomes. In addition, immediate transfer is associated with a nonsignificant trend to better clinical pregnancy rate and it also avoids the psychological effects of prolonging the stress on prospective parents.

     

A case of postpartum cerebral angiitis and review of the literature

Purpose  

To better characterize postpartum cerebral angiitis (PPCA).     

Evaluation of outcomes after transabdominal cervicoisthmic cerclage

Purpose  

To evaluate maternal and neonatal outcomes after transabdominal cerclage.     

Effectiveness of high‐dose transvaginal progesterone supplementation for women who are undergoing a frozen‐thawed embryo transfer

Purpose

To evaluate the effectiveness of high‐dose progesterone supplementation for women who are undergoing a frozen‐thawed embryo transfer (FET).

Methods

Among the 2010 FET cycles that were included in the present study, 1188 were 1200 mg/d of vaginal progesterone, while 822 were 900 mg/d. The dose of progesterone that was used was decided by the treatment period and additional progesterone supplementation was used when the serum progesterone levels were <9 ng/mL on luteal day 5.

Results

The clinical pregnancy rate was higher in the 1200 mg group than in the 900 mg group. The mean serum progesterone level on luteal day 5 in the 1200 mg and 900 mg groups was 12.6 ng/mL and 13.4 ng/mL, respectively. The rate of additional progesterone supplementation was higher in the 1200 mg group. A logistic regression analysis identified a younger age (≤37 years) and the use of 1200 mg progesterone as independent predictive factors for the clinical pregnancy outcome. The analysis of the infant outcomes revealed no significant difference in the distribution of birth ages and weights.

Conclusion

High‐dose transvaginal progesterone of 1200 mg/d as luteal support contributed to good pregnancy outcomes.     

In vitro maturation of oocytes via the pre-fabricated self-assembled artificial human ovary

Purpose  

Create a 3-Dimensional artificial human ovary to mature human oocytes.     

Risk factors and microbial isolates of puerperal sepsis at the University of Maiduguri Teaching Hospital,Maiduguri, North-eastern Nigeria

Purpose  

To determine the risk factors and microbial isolates of puerperal sepsis.     

Effects of cumulus cells removal after 6 h co-incubation of gametes on the outcomes of human IVF

Purpose  

To investigate the effects of cumulus cells removal after 6 h co-incubation of gametes on the fertilization, polyspermy, multinucleation and clinical pregnancy rates in human IVF.     

Huge lipoleiomyoma of the uterine cervix

Introduction  

Vulvar sarcomatoid squamous cell carcinoma has been rarely reported.     

Effect of preantral mouse follicle culture period on meiotic maturation and developmental competence of oocytes

Purpose  

The aim of this study is to determine the optimal culture period for meiotic maturation and developmental competence of in vitro-grown mouse oocytes.     

Antenatal three-dimensional sonographic features of Roberts syndrome

Introduction  

We present a fetus whose characteristics most likely represent Roberts syndrome.     

Improved developmental potential of mouse vitrified-warmed oocytes achieved by culturing in recovery medium with glutathione ethyl ester (GSH-OEt)

Purpose

The aim of the present study was to investigate the effect of glutathione ethyl ester (GSH-OEt) in the recovery medium on the developmental competence of mouse vitrified-warmed MII oocytes.

Methods

Vitrified-warmed oocytes were incubated for 1 h in recovery medium in the presence or absence of 0.5 mM GSH-OEt. The authors examined the effects of GSH-OEt, first on the levels of glutathione (GSH) and reactive oxygen species (ROS) in vitrified-warmed oocytes, and second, on in vitro blastocyst development, division speed to blastocysts, and total cell numbers of blastocysts from vitrified-warmed oocytes fertilized by Intracytoplasmic sperm injection (ICSI).

Results

Adding GSH-OEt to the recovery medium significantly (p < 0.05) increased GSH content and decreased ROS levels in vitrified-warmed oocytes. The blastocyst rate did not differ significantly between the two groups, but the speed of development to blastocysts in the GSH-OEt (+) group was significantly more rapid. In addition, the total blastocyst cell number was significantly higher in the GSH-OEt (+) group than in the GSH-OEt (−) group (92.8 ± 5.1 vs. 71.4 ± 3.5, p < 0.01).

Conclusion

Adding GSH-OEt to the recovery medium of vitrified-warmed mouse oocytes enhances the development potential of oocytes and improves the quality of blastocysts.

     

Pregnancy outcome and fertility after complete uterine rupture: a report of 20 pregnancies and a review of literature

Objective  

To review the pregnancy outcome in women with previous complete uterine rupture.     

Critical analysis of risk factors and outcome of placenta previa

Objective  

To investigate risk factors and pregnancy outcome of patients with placenta previa.     

Influence of the vascular endothelial growth factor on the development of severe pre-eclampsia or HELLP syndrome

Purpose  

The sexual activity and contraceptive use is evaluated over 21 years amongst Danish adolescents in ninth grade.     

Case report of a sarcoma botryoides of the uterine cervix in fertile age and literature review

Introduction  

The sarcoma botryoides of the cervix uteri occurs very rarely in fertile women.     

Sexual experience and risky sexual behaviours of Turkish university students

Objectives  

To determine the sexual experience and risky sexual behaviours of Turkish university students.     

Prenatal diagnosis of skeletal dysplasia due to <Emphasis Type="Italic">FGFR3</Emphasis> gene mutations: a 9-year experience

Purpose  

Prenatal diagnosis with ultrasound findings compatible with skeletal dysplasia due to FGFR3 mutations over a 9 year period in pregnancies and abortuses.     

Metastatic neoplasms of the ovaries: a clinicopathological study of 97 cases

Objective  

To present the clinicopathological features of metastatic ovarian neoplasms with emphasis in the diagnostic challenge.     

Surgery and cancer after endometrial resection. Long-term follow-up on menstrual bleeding and hormone treatment by questionnaire and registry

Purpose  

Long-term follow-up 11 years after endometrial ablation.