共查询到20条相似文献,搜索用时 15 毫秒
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H. Shindo S. Maekawa K. Komase M. Miura M. Kadokura R. Sueki N. Komatsu K. Shindo F. Amemiya Y. Nakayama T. Inoue M. Sakamoto A. Yamashita K. Moriishi N. Enomoto 《Journal of viral hepatitis》2013,20(4):281-289
Genetic variation in the IL‐28B (interleukin‐28B; interferon lambda 3) region has been associated with sustained virological response (SVR) rates in patients with chronic hepatitis C treated with peginterferon‐α and ribavirin. However, the mechanisms by which polymorphisms in the IL‐28B gene region affect host antiviral responses are not well understood. Using the HCV 1b and 2a replicon system, we compared the effects of IFN‐λs and IFN‐α on HCV RNA replication. The anti‐HCV effect of IFN‐λ3 and IFN‐α in combination was also assessed. Changes in gene expression induced by IFN‐λ3 and IFN‐α were compared using cDNA microarray analysis. IFN‐λs at concentrations of 1 ng/mL or more exhibited concentration‐ and time‐dependent HCV inhibition. In combination, IFN‐λ3 and IFN‐α had a synergistic anti‐HCV effect; however , no synergistic enhancement was observed for interferon‐stimulated response element (ISRE) activity or upregulation of interferon ‐ stimulated genes (ISGs). With respect to the time course of ISG upregulation, the peak of IFN‐λ3‐induced gene expression occurred later and lasted longer than that induced by IFN‐α. In addition, although the genes upregulated by IFN‐α and IFN‐λ3 were similar to microarray analysis, interferon‐stimulated gene expression appeared early and was prolonged by combined administration of these two IFNs. In conclusion, IFN‐α and IFN‐λ3 in combination showed synergistic anti‐HCV activity in vitro. Differences in time‐dependent upregulation of these genes might contribute to the synergistic antiviral activity. 相似文献
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Interventions to significantly improve service uptake and retention of HIV‐positive pregnant women and HIV‐exposed infants along the prevention of mother‐to‐child transmission continuum of care: systematic review
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Alexandra C. Vrazo Jacqueline Firth Anouk Amzel Rebecca Sedillo Julia Ryan B. Ryan Phelps 《Tropical medicine & international health : TM & IH》2018,23(2):136-148
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Recent studies have associated genetic variation near the interleukin 28B (IL28B/IFN‐λ3) gene with natural clearance of the hepatitis C virus (HCV) infection, and a common variant in the DEP domain containing 5 (DEPDC5) locus on chromosome 22 has been shown to affect susceptibility to hepatocellular carcinoma (HCC) in Japanese individuals with chronic HCV infection. This study was conducted to determine whether polymorphisms near or in interferon‐lambda (IFN‐λs) genes and their receptor genes such as interleukin 28 receptor, alpha (IL28RA) and interleukin 10 receptor, beta (IL10RB) as well as p21_activated kinases 4 (PAK4) and iron/zinc purple acid phosphatase‐like protein (PAPL), which are locate upstream of IFN‐λs, and lastly the DEPDC5 gene are associated with hepatitis B virus‐related liver disease in Han Chinese. The study subjects included 507 normal healthy controls, 350 individuals with natural clearance of HBV and 792 HBV‐infected patients. The patients were categorized into 157 inactive carriers (Case I), 216 active carriers (Case II), 111 cirrhotics (Case III) and 308 HCC patients (Case IV) subgroups. Seven single nucleotide polymorphisms (SNPs) were genotyped using the Matrix‐assisted Laser Desorption/Ionisation mass spectrometric (MALDI‐TOF MS) SNP genotyping assay. Rs423058 upstream of PAPL, rs2834167 in IL10RB and rs1012068 in DEPDC5 were associated with chronic HBV status, HBV natural clearance and the presence of HCC (P = 0.0004–0.024), respectively. PAPL, IL10RB and DEPDC5 polymorphisms have an impact on progression of HBV‐related liver disease. However, IFN‐λs genes as a tool to differentiate between different clinical courses of HBV infection were not useful in the Han Chinese population. 相似文献
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Relative expression analysis of IL‐5 and IL‐6 genes in tropical sheep breed Pelibuey infected with Haemonchus contortus
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Z. M. Estrada‐Reyes A. G. López‐Reyes A. Lagunas‐Martínez G. Ramírez‐ Vargas S. Olazarán‐Jenkins J. Hernández‐Romano P. Mendoza‐de‐Gives M. E. López‐Arellano 《Parasite immunology》2015,37(9):446-452
Haemonchus contortus is a parasitic nematode of Pelibuey sheep, a meat breed used in tropical regions. Due to anthelmintic problems, the identification of hosts resistant to H. contortus is another option of control. The aim of this study was to analyse the relative expression of IL‐5 and IL‐6 genes in Pelibuey sheep after H. contortus infection. Nineteen lambs infected with H. contortus and three more lambs without infection were studied. The haemonchosis was determined by the number of eggs per gram of faeces (epg) and by the estimation of the percentage of the packed cell volume (%pcv). Peripheral blood mononuclear cells (PBMCs) were obtained to extract RNA at 0, 1, 2, 7, 14, 21 and 28 days after infection to quantify the relative expression of IL‐5, IL‐6 and GAPDH by real‐time PCR. Five lambs were classified as low responders (lr) to haemonchosis with averages of 1519 ± 315·3 epg and 31·49 ± 5·13%pcv, and 14 lambs were identified as high responders (hr) with averages of 530 ± 132 epg and 34·88 ± 3·75%pcv. The expression ratio of IL‐5 was significantly different compared with control lambs at 2, 7 and 14 days post‐infection (PI), and IL‐6 was significantly different after 14 days. The highest level of relative expression for IL‐5 and IL‐6 genes was 9·9‐fold and 12‐fold after 2 and 14 days for hr hosts (P < 0·05) compared with control group, respectively. In conclusion, the Pelibuey breed in grazing areas exhibited different expression of IL‐5 and IL‐6 obtained from PBMCs against H. contortus, suggesting the importance of these cytokines in regulating the nematode infection. 相似文献
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Benjamin Heidrich Beatriz C. Serrano Ramazan Idilman Gökhan Kabaçam Birgit Bremer Regina Raupach Fatih O. Önder Katja Deterding Behrend J. Zacher Andrzej Taranta Hakan Bozkaya Kalliopi Zachou Hans L. Tillmann Abdurrahman M. Bozdayi Michael P. Manns Cihan Yurdaydın Heiner Wedemeyer 《Liver international》2012,32(9):1415-1425
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Mario Luppi Ivana Lagreca Patrizia Barozzi Chiara Quadrelli Daniela Vallerini Eleonora Zanetti Sabrina Basso Fabio Forghieri Monica Morselli Monica Maccaferri Ambra Paolini Valeria Fantuzzi Andrea Messerotti Rossana Maffei Ilaria Iacobucci Giovanni Martinelli Roberto Marasca Franco Narni Patrizia Comoli Leonardo Potenza 《British journal of haematology》2014,164(2):299-302
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Nirav N. Shah Aniko Szabo Scott F. Huntington Narendranath Epperla Nishitha Reddy Siddhartha Ganguly Julie Vose Cynthia Obiozor Fahad Faruqi Alexandra E. Kovach Luciano J. Costa Ana C. Xavier Ryan Okal Abraham S. Kanate Nilanjan Ghosh Mohamed A. Kharfan‐Dabaja Lauren Strelec Mehdi Hamadani Timothy S. Fenske Oscar Calzada Jonathon B. Cohen Julio Chavez Jakub Svoboda 《British journal of haematology》2018,180(4):534-544
Primary mediastinal (thymic) large B‐cell lymphoma (PMBCL) is an uncommon subtype of non‐Hodgkin lymphoma (NHL) that presents with a mediastinal mass and has unique clinicopathological features. Historically, patients with PMBCL were treated with R‐CHOP (rituximab, cyclophosphamide, doxorubicin, vincristine, prednisone) chemotherapy ± involved field radiation. Since a phase II trial, published in April 2013, demonstrated excellent results using dose‐adjusted (DA) R‐EPOCH (rituximab, etoposide, prednisone, vincristine, cyclophosphamide, doxorubicin), this treatment has gained popularity. We performed a retrospective, multicentre analysis of patients aged ≥18 years with PMBCL since January 2011. Patients were stratified by frontline regimen, R‐CHOP versus DA‐R‐EPOCH. 132 patients were identified from 11 contributing centres (56 R‐CHOP and 76 DA‐R‐EPOCH). The primary outcome was overall survival. Secondary outcomes included progression‐free survival, complete response (CR) rate, and rates of treatment‐related complications. Demographic characteristics were similar in both groups. DA‐R‐EPOCH use increased after April 2013 (79% vs. 45%, P < 0·001), and there was less radiation use after DA‐R‐EPOCH (13% vs. 59%, P < 0·001). While CR rates were higher with DA‐R‐EPOCH (84% vs. 70%, P = 0·046), these patients were more likely to experience treatment‐related toxicities. At 2 years, 89% of R‐CHOP patients and 91% of DA‐R‐EPOCH patients were alive. To our knowledge, this represents the largest series comparing outcomes of R‐CHOP to DA‐R‐EPOCH for PMBCL. 相似文献
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Neutrophil‐To‐Lymphocyte Ratio: An Emerging Marker Predicting Prognosis in Elderly Adults with Community‐Acquired Pneumonia
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Emanuela Cataudella MD Chiara M. Giraffa MD Salvatore Di Marca MD Alfredo Pulvirenti PhD Salvatore Alaimo PhD Marcella Pisano MD Valentina Terranova MD Thea Corriere MD Maria L. Ronsisvalle MD Rosario Di Quattro MD Benedetta Stancanelli MD PhD Mauro Giordano MD Carlo Vancheri MD PhD Lorenzo Malatino MD 《Journal of the American Geriatrics Society》2017,65(8):1796-1801
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Comparison of glucose‐lowering agents after dual therapy failure in type 2 diabetes: A systematic review and network meta‐analysis of randomized controlled trials
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Francesco Zaccardi MD Nafeesa N. Dhalwani PhD Jolyon Dales BM BS Hamid Mani PhD Kamlesh Khunti FRCGP Melanie J. Davies FRCP David R. Webb FRCP 《Diabetes, obesity & metabolism》2018,20(4):985-997
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The anti‐lymphoma activity of antiviral therapy in HCV‐associated B‐cell non‐Hodgkin lymphomas: a meta‐analysis
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J. Peveling‐Oberhag L. Arcaini K. Bankov S. Zeuzem E. Herrmann 《Journal of viral hepatitis》2016,23(7):536-544
Many epidemiological studies provide solid evidence for an association of chronic hepatitis C virus (HCV) infection with B‐cell non‐Hodgkin's lymphoma (B‐NHL). However, the most convincing evidence for a causal relationship between HCV infection and lymphoma development is the observation of B‐NHL regression after HCV eradication by antiviral therapy (AVT). We conducted a literature search to identify studies that included patients with HCV‐associated B‐NHL (HCV‐NHL) who received AVT, with the intention to treat lymphoma and viral disease at the same time. The primary end point was the correlation of sustained virological response (SVR) under AVT with lymphoma response. Secondary end points were overall lymphoma response rates and HCV‐NHL response in correlation with lymphoma subtypes. We included 20 studies that evaluated the efficacy of AVT in HCV‐NHL (n = 254 patients). Overall lymphoma response rate through AVT was 73% [95%>confidence interval, (CI) 67–78%]. Throughout studies there was a strong association between SVR and lymphoma response (83% response rate, 95%>CI, 76–88%) compared to a failure in achieving SVR (53% response rate, 95%>CI, 39–67%, P = 0.0002). There was a trend towards favourable response for AVT in HCV‐associated marginal zone lymphomas (response rate 81%, 95%>CI, 74–87%) compared to nonmarginal zone origin (response rate 71%, 95%>CI, 61–79%, P = 0.07). In conclusion, in the current meta‐analysis, the overall response rate of HCV‐NHL under AVT justifies the recommendation for AVT as first‐line treatment in patients who do not need immediate conventional treatment. The strong correlation of SVR and lymphoma regression supports the hypothesis of a causal relationship of HCV and lymphomagenesis. 相似文献
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