桥粒芯糖蛋白1、3在HaCaT细胞、A431细胞及原代角质形成细胞中的表达

【摘要】 目的 测定不同类型角质形成细胞（KC）桥粒芯糖蛋白（DSG）1、DSG3及DSG1 mRNA、DSG3 mRNA表达。 方法 不同KC（HaCaT细胞、A431细胞及原代KC）培养后,直接免疫荧光观察细胞DSG1、DSG3的表达,定量聚合酶链反应（qPCR）测定DSG1 mRNA、DSG3 mRNA相对表达水平。 结果 DSG1与DSG3表达于三种角质形成细胞,荧光强度显示,A431细胞高于HaCaT细胞,HaCaT细胞高于原代KC。三种细胞均表达DSG1、DSG3 mRNA,原代KC DSG1与DSG3 mRNA的相对表达量分别为HaCaT细胞的291.7%及237.4%,差异有统计学意义（P < 0.01）;A431细胞DSG1 mRNA为HaCaT细胞的0.1%、DSG3 mRNA则为HaCaT细胞的18.8%,差异有统计学意义（P < 0.05）。 结论 三种KC均可用于对DSG1、DSG3的相关研究;相对而言,正常培养的A431细胞表面DSG1、DSG3表达较HaCaT细胞及原代KC高;原代KC DSG1、DSG3 mRNA水平较HaCaT细胞及A431细胞高。     

UBA3在黑素瘤细胞的表达及其影响黑素瘤细胞生物学行为的研究

【摘要】 目的 探讨UBA3在黑素瘤细胞中的表达,初步观察干扰UBA3对黑素瘤细胞生物学行为的影响。 方法 用RT-PCR技术观察3株黑素瘤细胞中UBA3的表达,并利用siRNA技术对M14细胞中UBA3的表达进行干扰,检测转染后细胞内UBA3、连接态NEDD8以及p53的表达,并观察转染前后细胞的凋亡及迁移能力的改变。 结果 在A375、M14及MV3中UBA3的表达均较正常黑素细胞上调,其中M14细胞上调最明显,成功干扰M14中UBA3的表达后,细胞内连接态NEDD8的表达下降,p53的表达明显增加,同时M14细胞凋亡增加、迁移能力下降。 结论 构建的质粒可干扰UBA3的表达,影响细胞的Nedd化进程,从而影响细胞的生物学行为。     

带状疱疹患者外周血T细胞及NK细胞检测

目的:评价机体免疫功能与带状疱疹的相关性。方法:选取25例带状疱疹患者和30名对照者,流式细胞术检测CD4~+T细胞、CD3~+T细胞、CD8~+T细胞、CD4/CD8及NK细胞。结果:与对照组相比较,患病组CD4~+T细胞、CD3~+T细胞降低,NK细胞升高。差异有统计学意义(P0.05)。结论:带状疱疹存在免疫功能异常。     

白癜风中的T细胞像

白癜风的发病学,现代倾向于与免疫介导的黑素细胞破坏有关。支持此种理论的证据包括本病常伴有其它自身免疫性疾病;血行中存在器官特异性自身抗体;证明有抗黑素细胞抗体及皮损部位基底膜带有IgG及C_3的沉积。本文对20例白癜风患者及16名健康对照者作了研究,应用间接免疫荧光法及补体介导细胞毒性试验测定周围血总T细胞(OKT_3)、辅助性T细胞(O     

基底细胞上皮癌

基底细胞上皮癌是皮肤癌中发病率最高者,约占55～70%,男∶女为3∶2.患者多在60岁以上,近几年,平均年龄在降低.易侵犯白色及棕黄色皮肤的人种,黑种人很少发病.可发于任何部位,绝不侵犯粘膜.95%的病例位于面部、头皮及颈部,5%     

发生于皮肤纤维瘤上的基底细胞癌和基底细胞癌样变

作者报告3例发生于皮肤纤维瘤基础上的色素沉着结节性基底细胞癌和19例基底细胞癌样变。这3例色素沉着结节性基底细胞癌患者一男二女,其年龄是40、44、46岁,损害大小为10×13毫米至1.4厘米,病期较长可达10年之久,临床诊断为恶性黑色素瘤、血管瘤及痣等。在切除后活检发现为硬化性血管瘤型皮肤纤维瘤、硬化性血管瘤及皮肤纤维瘤,在此基础上均能见到数个境界整齐的,由圆形或卵圆形嗜碱性细胞组成的岛屿状结构,其边缘有栅状细胞排列与附近真皮隔开或与表皮相连,有的边缘皱缩出现空隙,岛屿状肿瘤中有色素堆积。以上3例是结节性基底钿胞癌位于皮肤纤维瘤上。     

Merkel细胞癌一例

Merkel细胞癌是一种罕见的皮肤恶性肿瘤 ,好发于暴露部位 ,有明显局部复发及早期转移倾向。我们见到一例 Merkel细胞癌 ,并出现眼睑、额部及胸部等多处转移 ,现报道如下。 患者男 ,66岁 ,2年前发现左前臂伸侧有一肿物 ,2.5 cm× 2.5 cm× 3 cm,质硬 ,活动 ,左上肢感觉运动正常 ,腋窝淋巴结不大。行手术切除 ,病理示表皮正常 ,真皮乳头层无浸润 ,真皮中下层广泛片状及条带状单一核细胞浸润 ,细胞核呈圆形、椭圆形 ,较一致 ,灰蓝染色 ,胞浆稀少 ,胞膜不明显 ,伴淋巴细胞浸润 ,可见分裂象（图 1,2）。免疫组化示突触素 (SY38)(＋ )(图 3)、嗜…     

获得性大疱性表皮松解症合并鳞状细胞癌

患者女性,43岁。10年来肢端及摩擦部位反复发生大疱,尼氏征阳性,并发口、眼损害。组织病理示表皮下大疱、直接免疫荧光示沿基底膜有 IgG、C3呈线状沉积,电镜示致密板下裂隙,据此诊为 EBA。患者自1981年开始接受皮质类固醇治疗(初始剂量1mg/kg/d)后水疱减少,但肢端仍形成萎缩性瘢痕并发眼部损害(睑内翻)。此后,给予强的松龙0.4mg/d 口服维持,未再出现明显的皮肤及粘膜的大疱性损害。约1年来,右手腕屈侧出现直径约3cm,浸润性及角化性的肿块,无周围淋巴结肿大及     

雄激素性秃发患者毛乳头细胞基因表达谱分析

【摘要】 目的 探讨3D培养下,雄激素性秃发患者毛乳头细胞基因表达谱的差异。 方法 分离雄激素性秃发患者毛乳头细胞进行3D培养,实验组经双氢睾酮处理,提取总RNA进行扩增,Cy3标记,用NimbleGen人类全基因组表达谱芯片杂交,筛选差异表达基因进行GO分析及pathway分析,实时 PCR对结果进行验证。结果 全基因组表达谱分析显示,有622 个基因有差异性表达,上调基因数359个,下调基因数为263个。其中GO分析显示,抑制细胞增殖、促进凋亡的基因出现上调,如CHEK1及Tob1等。促进细胞增殖、参与表皮生长发育的分子表达下调,如BAMBI、EFNA3、Dlx3及UCGC等。实时 PCR验证结果与芯片结果的差异趋势一致。Pathway分析显示,调节细胞周期信号转导通路的分子富集在首位。 结论 雄激素性秃发发病受多种信号分子及信号转导通路调节,可能集中在调节细胞周期、细胞增殖及凋亡等方面。     

皮下脂膜炎样T细胞淋巴瘤1例

患者女,46岁。双小腿肿胀、结节伴疼痛5个月。组织病理及免疫组化确诊为皮下脂膜炎样T细胞淋巴瘤。经CHOP方案治疗3个疗程后,皮疹及全身症状未见好转,3个月后死亡。     

Lymphomatoid Papulosis Followed by Anaplastic Large Cell Lymphoma in a Pediatric Patient

Lymphomatoid papulosis (LyP) is a benign, self-healing, papular eruption that can wax and wane over time. Transformation to T-cell lymphoma has been well documented in 10% to 20% of adults with LyP. However, this transformation rarely occurs in patients younger than 20 years of age. Here, we present the first known pediatric patient in Korea, a 12-year-old boy who developed a subcutaneous nodule on the scrotum 13 months after papulonecrotic lesions of LyP were identified on both lower extremities and face. Histological and immunohistochemical examination of the subcutaneous nodule revealed anaplastic large cell lymphoma (ALCL). A T-cell receptor gene rearrangement analysis demonstrated an identical rearranged pattern in the two specimens, indicating that a common T-cell clone had proliferated over time in both the LyP and ALCL lesions.     

Treatment of primary cutaneous B cell lymphoma with local radiotherapy

A patient with primary cutaneous B cell lymphoma that presented as a solitary nodule on the abdomen was successfully treated with local radiotherapy. A brief review of special studies used to diagnose this tumor as well as clinical characteristics of the tumor is presented.     

Cutaneous Sparganosis: A Case Report and Literature Review

A sixty‐nine‐year‐old male patient, without a significant prior medical history, presented with a rapidly enlarging, 2 cm non‐tender nodule on the right lower eyelid. The lesion persisted in spite of an incision and drainage. Following an excision, the histology revealed a diffuse infiltrate of atypical mononuclear cells within the dermis. The cells were large, monomorphic, with irregular to convoluted nuclei, prominent nucleoli, amphophilic cytoplasm. They were very mitotically active. Immunohistochemical stains for CD45, CD3, and CD30 were strongly positive. Pancytokeratin, CD20, and ALK (Anaplastic Lymphoma Kinase fusion protein) were negative. The histopathologic diagnosis was CD30 (Ki‐1) positive anaplastic large cell lymphoma. The margin was positive for involvement by lymphoma. The lesion was re‐excised with no evidence of residual involvement by lymphoma. A primary cutaneous anaplastic large cell lymphoma was favored over systemic involvement based on the clinical presentation of a single eyelid nodule and ALK negativity. He was referred to a hematology‐oncologist for further management. Anaplastic large cell lymphoma is a distinctive type of malignant lymphoma with a relatively favorable prognosis. It frequently involves the skin, however, to the best of our knowledge, presentation of this lymphoma type as a single lesion on the eyelid is extremely rare.     

Sudden onset of an aggressive cutaneous lymphoma in a young patient with psoriasis: role of immunosuppressants

Psoriasis is thought to be associated with an increased risk of lymphoma. We report here the first case of an aggressive primary cutaneous pleomorphic T-cell lymphoma in a patient with psoriasis. The 36-year-old patient, who had previously been treated successively with methotrexate, ciclosporin and etanercept, presented with rapidly growing nodules on the leg. A biopsy confirmed a stage IVa primary cutaneous pleomorphic T-cell lymphoma. Despite treatment with pegylated liposomal doxorubicin, the disease progressed and the patient died 5 months later. This case of pleomorphic T-cell lymphoma was remarkable in both its extremely rapid onset and the aggressive nature of the disease. The onset of this disease in a patient with psoriasis who had been previously treated with immunosuppressive drugs and a tumour necrosis factor (TNF)-α blocker is of major interest. Only eight cases of cutaneous lymphomas associated with treatment with TNF-α blockers have been published previously. Most of these eight cases related to anti-TNFα antibodies; only two were linked to etanercept.     

Oral-cutaneous CD4-positive T-cell lymphoma: a study of two patients

We describe two slowly progressive cases of T-cell lymphoma that involved both acral skin and oral cavity. One patient presented with a tongue nodule, completely responded to chemotherapy and then developed recurrent lymphoma involving tongue and skin a few months later that also responded to therapy. The second patient presented with a skin nodule that spontaneously resolved without therapy, and subsequently recurred in tongue and skin a few years later. In both cases, the neoplasms were composed of atypical lymphoid cells with epidermotropism and were of T-helper cell lineage (CD4+). The initial lesions were also negative for CD30. Identical T-cell receptor gene rearrangements were detected in the initial and recurrent lesions of one case. Although these neoplasms were classified as unspecified peripheral T-cell lymphoma because of the unusual distribution of disease, both cases also had histopathologic features of mycosis fungoides. These cases are strikingly similar, and may represent an unusual clinicopathologic type of T-cell lymphoma that can hone to cutaneous and oral mucosal sites with a slowly progressive natural history.     

CD20 Positive T Cell Lymphoma Involvement of Skin

CD20 positive T cell lymphoma is a rare condition that is associated with the coexpressions of CD20 and T cell markers, such as, CD3, CD5, or UCHL-1. Positivity for CD20 in this tumor represents an aberrant immunophenotype, but the presence of monoclonal T cell receptor (TCR) gene rearrangements and negativity for immunoglobulin heavy chain gene rearrangement indicate that this tumor is a T cell lymphoma. The majority of cases of CD20 positive T cell lymphoma have been reported as immature peripheral T cell lymphoma not otherwise specified. However, we believe that this disease is likely to be re-listed as a new disease entity after its pathogenesis has been elucidated and more cases have been evaluated. Here, we present a case of peripheral T cell lymphoma coexpressing CD20 and T cell markers with a demonstrable TCR gene rearrangement, in a patient who had been misdiagnosed as having B cell type lymphoma 4 years previously. We hypothesize that in this case initially circulating normal CD20+ T cell subsets underwent neoplastic transformation and CD20 positive T cell lymphoma subsequently developed in the lymph node, and then recurred in the skin due to systemic disease or metastasized from the nodal disease.     

Basal cell carcinomas and lymphoma:Biologic behavior and associated factors in sixty-three patients

The relationship between basal cell carcinoma and lymphoma was investigated in 63 patients with both diagnoses who were seen at Memorial Sloan-Kettering Cancer Center between 1949 and 1984. The majority of patients were diagnosed with lymphoma before the onset of their first basal cell carcinoma. Multiple basal cell carcinomas developed in most patients. The overall recurrence rate of basal cell carcinoma was high (17%), and metastatic disease developed in one patient. These data support a more aggressive behavior of basal cell carcinomas in patients with lymphoma than in otherwise healthy individuals.     

Fatal CD8+ epidermotropic cytotoxic primary cutaneous T-cell lymphoma with multiorgan involvement

A 70-year-old woman presented with a 3-month history of two ulcerated erythematous-violaceous nodular lesions over the nose and forehead, respectively. The patient's history included a similar cutaneous nodule on the glabella diagnosed as pseudolymphoma 2 years ago.At that time, despite the diagnosis of a benign disease, an adequate staging was performed, ruling out any extracutaneous involvement. During hospitalization, multiple purpuric papules developed over the abdomen, and the disease spread to mediastinal lymph nodes, lungs and the central nervous system. Based on the histologic, immunophenotypic and molecular biology findings, a diagnosis of CD8+ epidermotropic cytotoxic primary cutaneous T-cell lymphoma was made. Secondary skin involvement by a CD8+ extracutaneous T-cell lymphoma could not be excluded with certainty, but seemed to be unlikely because of the negativity of the initial workup. The patient died from complications of right femoral artery thrombosis before starting specific polychemotherapy 21 months after onset of the disease. Among primary cutaneous T-cell lymphomas, the CD8+ epidermotropic cytotoxic subset comprises rare, highly aggressive forms characterized by metastatic spread to unusual sites such as the oral cavity, lungs, testis and the central nervous system but usually not to the lymph nodes. These cases seem to be distinct from mycosis fungoides with CD8+ phenotype, which shows a nonaggressive clinical behavior.     

Chloroma (aleukaemic leukaemia cutis) initially diagnosed as cutaneous lymphoma

A 65-year-old man presented in 1997 with a nodule on his back; histology showed apparent high grade T-cell lymphoma, treated after excision with radiotherapy. He relapsed with lesions on the thigh and buttock in 1998 and was treated with CHOP chemotherapy with a complete response. Further relapse occurred in 1999 with a nodule on his thigh again; he received CNOP (doxorubicin substituted with mitozantrone). At no stage was there clinical, bone marrow or radiological evidence of extra cutaneous disease. In November 2000 he presented with widespread indurated plaques and violaceous nodules. Biopsies repeated with extensive immunohistological staining diagnosed aleukaemic leukaemia cutis. Our patient was diagnosed with cutaneous T-cell lymphoma (CTCL) on the basis of clinical and haemotoxylin & eosin appearances. The correct diagnosis was made after extensive immunohistological studies (including myeloid markers) of repeat biopsies. This case illustrates the importance of diagnostic review in atypical CTCL. There is a high incidence of progression to acute myeloid leukaemia.     

A rare case of the cutaneous form of adult T-cell leukaemia/lymphoma: assessment of remission by PCR for clonal T-cell receptor gamma gene rearrangements in an electron beam-irradiated cutaneous lesion

Adult T-cell leukaemia/lymphoma is a lymphoproliferative disorder aetiologically associated with human T-cell lymphotropic virus type I infection. A cutaneous lesion often develops in the disease, and in rare cases, is even the only manifestation. Here we report a rare case of 'cutaneous' adult T-cell leukaemia/lymphoma with neither atypical cells in the peripheral blood nor lymph node involvement. All nodular lesions were completely eliminated after local electron beam irradiation (20 Gy/nodule in total). To evaluate whether or not there were residual lymphoma cells in the skin, we performed PCR to detect clonal T cell receptor gamma gene rearrangements. The sample from the nodule before irradiation showed evidence of a rearranged band, which was not detected at the same site after treatment nor in any peripheral blood. The findings suggest that this procedure is useful for the evaluation of therapeutic effects and the early detection of lymphoma recurrence.