正常人,龋病和牙髓炎患者牙髓组织中一氧化氮含量的检…

目的：正常人，龋病和牙髓炎病患者牙髓组织中一氧化氮（Ｎｉｔｒｉｃｏｘｉｄｅ，ＮＯ）含量进行检测，初步探讨ＮＯ在牙髓组织自身修复中的作用。方法：采用荧光分光光度法检测其中亚硝酸根（ＮＯ２）含量，以间接确定ＮＯ含量。结果：正常牙髓组织中ＮＯ无性别差异（Ｐ〉０．０５），浅龋组与对照组ＮＯ无显著性差异（Ｐ〉０．０５），而深龋伴慢性牙髓炎组ＮＯ含量则显著高于对照组（Ｐ〈０．０５）。结论：牙髓组织中ＮＯ含量的     

一氧化氮在牙髓组织中的生物学作用

一氧化氮（ＮＯ）是左旋精氨酸在一氧化氮合成酶作用下生成胍氨酸的过程中所产生的无机小分子物质。它参与机体多种生理及病理过程,其中在牙髓血管紧张性调节,牙髓痛觉传递及牙髓炎症反应等过程中均起一定作用。     

一氧化氮在牙髓组织中的生物学作用

一氧化氮(NO)是左旋精氨酸在一氧化氮合成酶作用下生成胍氨酸的过程中所产生的无机小分子物质。它参与机体多种生理及病理过程,其中在牙髓血管紧张性调节、牙髓痛觉传递及牙髓炎症反应等过程中均起一定作用。     

急性牙髓炎牙髓血白细胞总数的临床研究

牙髓血白细胞总数及其与全身周围血白细胞总数间的关系,很少有学者进行过报道。本组随机抽取门诊急性牙髓炎开髓引流患牙2 0个,年龄18～2 5岁,男13例,女7例。磨牙17个,前磨牙3个。在严格隔湿下,检查已开髓引流患牙的牙髓血白细胞总数,同时检查该患者全身周围血白细胞总数。结果:急性牙髓炎牙髓血白细胞总数最低值为2 .6×10 9/L ,最高值为5 .0×10 9/L ,均值为3.10 5×10 9/L。全身周围血白细胞总数最低值为4 .1×10 9/L ,最高值为11.6×10 9/L ,均值为7.0 75×10 9/L。急性牙髓炎牙髓血白细胞总数和均值低于全身周围血白细胞总数和均值…     

细菌及内毒素致大鼠实验性牙髓炎的牙髓组织病理学研究

目的 建立细菌及内毒素快速诱导大鼠实验性牙髓炎模型的方法。方法 采用口腔细菌与革兰氏阴性类杆菌ＬＰＳ联合运用,以产生大鼠实验性牙髓炎,并在其不同时期,观察牙髓组织的病理变化及疾病发展过程。结果（１）细菌－ＬＰＳ混合组较之生理盐水组,除２４ｈ时间段上无明显差异外,其它各时间段上牙髓组织充血、水肿、炎性浸润均明显甚于生理盐水组。（２）牙髓内共存有纤维增生以及成纤维细胞的成牙本质细胞增殖分化等牙髓组织修     

炎症人牙髓组织渗出液中IL-8含量的检测

目的：检测人正常和炎症牙髓组织渗出液中ＩＬ－８的含量,揭示ＩＬ－８与牙髓炎的关系。方法：用滤纸条浸润法采集正常和临床诊断为急性或慢性牙髓炎患牙牙髓组织渗出液。用ＥＬＩＳＡ方法检测其ＩＬ－８含量。结果：正常牙髓组织渗出液中检测不到ＩＬ－８,而炎症牙髓组织渗出液中均有较高水平的ＩＬ－８（０．３３～１．０μｇ／Ｌ）,且在急性牙髓炎中的水平高于慢性牙髓炎（Ｐ〈０．０１）。结论：ＩＬ－８参与了牙髓炎的发生和     

正常及炎症牙髓组织中IL—6的表达

目的：通过检测ＩＬ－６在人牙髓组织中的表达,探讨ＩＬ－６与正常及炎症牙髓组织的关系,方法：采用免疫组化（ＡＢＣ）法,检测正常组８个牙及炎症牙髓组１０个牙中ＩＬ－６的表达。结果：正常人牙髓组织ＩＬ－６染色为阴性,炎症牙髓组织中ＩＬ－６染色 呈阳性表达细胞为单核－巨噬细胞,少量血管内皮细胞和成纤维细胞。结论：ＩＬ－６与牙髓组织的炎症反应密切相关,是重要的炎症介质之一。     

正常牙髓和慢性炎症牙髓免疫球蛋白含量测定

对正畸或美观要求拔除的牙齿的牙髓和慢性牙髓炎免疫球蛋白及补体量进行测定,其结果对探讨免疫反应在牙髓病中的作用有一定意义。     

正常及炎症牙髓组织中IL-6的表达

目的：通过检测ＩＬ－６在人牙髓组织中的表达,探讨ＩＬ－６与正常及炎症牙髓组织的关系。方法：采用免疫组化（ＡＢＣ）法,检测正常组８个牙及炎症牙髓组１０个牙中ＩＬ－６的表达。结果：正常人牙髓组织ＩＬ－６染色为阴性,炎症牙髓组织中ＩＬ－６染色均呈阳性,主要表达细胞为单核－巨噬细胞,少量血管内皮细胞和成纤维细胞。结论：ＩＬ－６与牙髓组织的炎症反应密切相关,是重要的炎症介质之一。     

Intracellular complex carbohydrates in the normal and inflamed dental pulp

abstract — Forty-four inflamed pulps from human and monkey teeth were studied using histochemical staining methods. In pulps from carious human teeth, mast cells and many fibroblasts containing glycogen-like granules were regularly observed. In monkey pulps, where pulpitis was induced experimentally, mast cells were found only occasionally, and glycogen-like granules were absent. However, some fibroblasts in normal and inflamed monkey pulps contained periodic acid-Schiff (PAS) positive granules, which were resistant to amylase. Such granules were not found in human pulp fibroblasts.     

大鼠正畸牙移动中牙髓iNOS表达的免疫组化研究

目的:建立大鼠正畸牙移动模型,观察牙髓组织中诱导型一氧化氮合酶(iNOS)的表达及分布,探讨正畸牙移动过程中牙髓改建的分子机制。方法:采用免疫组织化学方法对正畸加力后12h、1d、3d、7d和14d大鼠牙髓组织中iNOS进行检测,观察iNOS的时空分布。结果:iNOS阳性反应的产物呈深褐色均质沉淀,主要在血管内皮细胞、成牙本质细胞胞浆核周区颗粒状阳性表达。这种染色在正畸加力后12h、1d、3d天有不同程度的增强,3d达到高峰,加力后7d和14d表达减弱,第14d与对照组无明显差异。结论:正畸牙移动过程中牙髓组织iNOS的表达先升高后逐渐恢复正常,提示iNOS可能在正畸牙移动牙髓组织改建过程中起重要作用。     

Identification of lymphocyte antigens in human dental pulps

The aim of the experiment was to study the immune reaction of cells in both healthy and inflamed human dental pulps. For this it was necessary to identify, in particular, populations of lymphocytes in the pulp, and also other cells involved in the immune reaction. By using monoclonal antibodies which specifically recognize subpopulations of the T lymphocytes, we were able: 1) to directly determine the phenotype of the cells of the pulp, 2) to examine the interactions of the different cell populations. The study was based on 30 permanent human dental pulps and the method was that of indirect immunofluorescence on frozen sections. The monoclonal antibodies used were the antibodies OKT and anti-Leu of types anti-Leu1, anti Leu3a, OKT4, OKT8 and OK1a. The results obtained were evaluated statistically. Two important points stand out from this study: 1) in the healthy samples there is preferential localization of lymphocytes at the periphery of the pulp and 2) there is a predominance of the OKT8+ cytotoxic suppressor subpopulation in both the healthy and the inflamed pulps.     

Catalase activity in human healthy and inflamed dental pulps

AIM: To examine the catalase activity in clinically healthy and symptomatic human dental pulps to verify if an active defence system against oxidizing agents is present as a response to bacterial invasion. METHODOLOGY: Thirty-three systemically healthy patients, 18 females and 15 males (ages: 11.0-25.9 years; mean 18.8 +/- 3.6), were the source of the pulp tissue. The condition of the pulps was assessed using clinical and radiographic evaluations. The specimens were recovered by longitudinally grooving and splitting the teeth (if extracted) or during endodontic treatment, and were matched for age and sex between the healthy and inflamed specimen groups. Catalase activity was determined through spectrophotometric methods. RESULTS: Enzymatic activity was 1126 +/- 343 and 3074 +/- 698 mU mL(-1) x mg of total protein in the healthy and inflamed pulp tissue specimens, respectively. This difference was statistically significant (P<0.01). CONCLUSIONS: These results indicate a role for catalase during dental pulp inflammation in humans, and may represent an inherent biological defence system against reactive oxidants of this tissue.     

人正常牙髓和炎症牙髓中P物质和血管内皮生长因子的表达

目的:研究P物质(SP)和血管内皮生长因子(VEGF)在人正常、外伤初期、炎症早期、炎症晚期牙髓中的表达和相关性,探讨两者对牙髓微循环的调节作用。方法:选取因正畸或阻生需拔除的健康牙10颗作为正常组,选取同年龄阶段因外伤需要根管治疗的患牙29颗,分为外伤初期组、早期炎症组、晚期炎症组。采用免疫组织化学方法对各组中SP、VEGF进行组织学定位和定量检测。采用SSPS13.0软件包对各组数据进行单因素方差分析和相关分析。结果:与正常组相比,SP在其他各组中表达显著降低(P<0.05);VEGF在早期炎症组中表达较其他各组强(P<0.05),而在晚期炎症组则明显减弱(P<0.05);SP和VEGF在各组中的表达呈负相关(r=-0.378,P<0.05)。结论:SP可能通过VEGF途径引起牙髓微循环的病理改变,两者共同参与牙髓神经源性炎症的发生。     

大鼠磨牙钻磨后牙髓中肌动蛋白表达的研究

目的：探讨肌动蛋白在大鼠正常牙髓及钻磨刺激后牙髓中的分布变化。方法：建立大鼠磨牙牙髓损伤模型，用免疫组化方法观察正常牙髓及钻磨后2h～14d牙髓中肌动蛋白的分布情况。结果：正常组成牙本质细胞及血管内皮细胞阳性；钻磨刺激2h组，穿髓点附近的成牙本质细胞转为阴性，血管内皮细胞及管周细胞强阳性；8h--5d各组可见强阳性血管在牙髓中分布并始终位于炎症发展的前沿区。结论：肌动蛋白对维持成牙本质细胞排列方式及正常生理功能有重要作用；其在血管内皮细胞及管周细胞的表达上调可能与牙髓损伤的早期修复有关。