解脲脲原体感染与精子凋亡的关系

解脲脲原体(Uu)是泌尿生殖道感染的常见病原体,国内外研究表明Uu感染与某些男性不育密切相关,但其机制尚未完全明了。其感染对精子凋亡影响研究报道尚少．特采用流式细胞术(FCM)对精液Uu培养不同结果的男性作精子凋亡率检测,探讨不育机理。     

解脲脲原体与宫内感染的相关性研究：Ⅰ.解脲脲原体感染与 …

目的 研究解脲脲原体与自然流产和药物不全流产的关系,探讨Ｕｕ与宫内感染的可能机制。方法 采用分离培养分别对４５例自然流产和２７例药物不全流产和３１例,人工流产的母亲宫颈分泌物及胚胎组织进行Ｕｕ检测,分别对部分试验组和对照组进行超薄切片,并与其培养物负染电镜检查相比较。     

解脲脲原体与宫内感染的相关性研究Ⅰ.解脲脲原体感染与自然流产及药物不全流产

目的研究解脲脲原体（Ｕｕ）与自然流产和药物不全流产的关系，探讨Ｕｕ与宫内感染的可能机制。方法采用分离培养分别对４５例自然流产和２７例药物不全流产（试验组）和３１例人工流产（对照组）的母亲宫颈分泌物及胚胎组织进行Ｕｕ检测，分别对部分试验组和对照组进行超薄切片，并与其培养物负染电镜检查相比较。结果试验组流产组织中Ｕｕ检出率分别为５３．３％和６３％，与对照组相比较，具显著性差异（ｐ＜０．００１，ｐ＜０．００１）；经超薄切片观察，试验组流产组织的绒毛膜下滋养层细胞周围见大量的Ｕｕ颗粒及空泡内增殖的子细胞，并与其流产组织中阳性培养物经负染后的颗粒相似。而对照组胚胎流产组织中无此颗粒。结论绒毛膜下滋养层Ｕｕ增殖这一结果充分证实了Ｕｕ是引起宫内感染的病原体之一。     

弓形体感染与唇腭裂关系的探讨

本文应用滤纸干血ELISA对山东部分城乡66名1岁内唇裂、腭裂患儿进行弓形体抗体测定。阳性率为25.76％,而对照组仅6.25％,两组率间有非常显著性差异(P<0.01)。提示弓形体感染可能是唇裂、腭裂发生的不可忽视的因素之一。     

孕妇弓形体感染与乙型肝炎病毒感染的关系

目的　探讨中期妊娠妇女弓形体的感染与乙型肝炎病毒感染的关系。方法　对 1 998年 1月至 2 0 0 3年1 2月来我院妇产科门诊进行产前检查的孕 1 3～ 1 6周的妊娠妇女共 45 87例进行了乙型肝炎病毒 ( HBV)和弓形体感染的筛查 ,并根据筛查结果分为 :HBV感染组和 HBs Ab阳性组 (选择注射乙肝疫苗者 ) ;同时随机抽取40 0例 HBs Ag、HBs Ab、HBe Ag、HBe Ab、HBc Ab均为阴性 ,无食用生肉或未煮熟的肉和猫类接触的正常孕妇为对照组。结果　HBV感染组中 ,孕妇既往有弓形体感染 ( Ig G阳性 )、活动性弓形体感染 ( Ig M阳性 )和复发性弓形体感染 ( Ig G和 Ig M均阳性 )的发生率分别为 1 0 .2 7%、6.1 1 %和 2 .93 % ,显著高于 Hbs Ab阳性组和对照组( P<0 .0 1 )。在排除食用生肉或未煮熟的肉类制品和同宠物猫类接触等因素的干扰后 ,HBV感染组中孕妇既往有弓形体感染、活动性弓形体感染和复发性弓形体感染的发生率分别为 1 2 .3 5 %、5 .98%和 3 .1 9% ,仍然明显高于 HBs Ab阳性组和对照组 ( P<0 .0 1 )。结论　 HBV感染是在食用生肉或未煮熟的肉类制品和同宠物猫类接触等因素之外使孕妇易于感染弓形体的因素之一     

宫颈解脲支原体感染与输卵管妊娠的关系探讨

目的探讨宫颈解脲支原体感染与输卵管妊娠的关系。方法对72例输卵管妊娠患者(观察组)和46例早孕妇女(对照组)进行宫颈分泌物解脲支原体培养,观察两组检测对象宫颈解脲支原体感染情况。结果输卵管妊娠患者宫颈分泌物解脲支原体阳性率为44.4%,对照组宫颈分泌物解脲支原体阳性率为21.7%,两组解脲支原体感染率间差异有统计学意义(P<0.01)。结论宫颈解脲支原体感染与输卵管妊娠有较密切的关系,防治解脲支原体是减低输卵管妊娠发病率和彻底治疗输卵管妊娠的重要措施之一。     

解脲支原体与不育症的关系

近年来的研究发现,解脲支原体(UU)是泌尿生殖系感染的常见病原菌。为探讨UU与不育症的关系,我们对238例不育患者的生殖系统分泌物进行检测,现报告如下。1 临床资料1.1 检测对象 不育组238例,均为结婚一年以上、有正常性生活、未避孕、月经正常、经检查卵巢能正常排卵而未孕者,其中65例合并盆腔炎。A组:UU阳性不孕妇女的性伴侣。B组:UU阴性不孕妇女的男性伴侣。对照组138例,为正常已生育妇女,其中36例合并盆腔炎。1.2 标本采集 女性,用无菌棉拭子直接拭取宫颈分泌物;男性,将棉拭子插入尿道约3cm或行前列腺按摩取尿道分泌物。所取标本分别置于无菌试管中,备检。1.3 检测方法 采用多聚酶链反应(PCR)     

胎膜早破与解脲支原体感染的关系

1999年9月～2000年2月,我院共收治胎膜早破35例,现就胎膜早破产褥病率与解脲支原体(UU)感染的关系进行分析。     

Occurrence and antimicrobial susceptibility of Ureaplasma parvum (Ureaplasma urealyticum biovar 1) and Ureaplasma urealyticum (Ureaplasma urealyticum biovar 2) from patients with adverse pregnancy outcomes and normal pregnant women

A recent phylogenetic analysis of Ureaplasma urealyticum resulted in the proposal to divide their 2 biovars into species. We used PCR to compare the distribution of species and the presence of the tet(M) and int-Tn resistance determinants in 63 strains of Ureaplasma spp. isolated from the amniotic fluid of patients with an adverse pregnancy outcome and in 22 strains obtained from the lower genital tract of healthy pregnant women. We also determined the antimicrobial susceptibility of the organisms to erythromycin and tetracycline. U. parvum was the most frequent Ureaplasma species detected in our study. Thus, 50/63 (79.4%) invasive isolates and 17/22 (77.3%) lower genital tract isolates corresponded to U. parvum, whereas 12/63 (19%) invasive isolates and 4/22 (18.2%) non-invasive strains corresponded to U. urealyticum. A mixture of species was found in 2 women. We found no significant differences in the antimicrobial susceptibility of isolates according to species or origin of isolation. Sixty-two strains of Ureaplasma spp. (74.7%) were susceptible to erythromycin, and 21 strains (25.3%) were intermediately susceptible. Sixty-eight isolates (81.9%) were susceptible to tetracycline, 2 strains (2.4%) were intermediate and 13 strains (15.7%) were resistant. DNA sequences related to the tet(M) determinant and the int-Tn gene were found in all tetracycline-resistant isolates.     

Disseminated Ureaplasma urealyticum infection in a hypo-gammaglobulinaemic renal transplant patient

We report of a case of severe disseminated U. urealyticum infection in a 35-y-old kidney transplanted patient with CVID. Routine microbiological tests were negative. Six weeks after admission, abscess material was grown on Mycoplasma culture medium yielding U. urealyticum in high titres. The patient responded promptly to appropriate antibiotics.     

不孕患者解脲支原体和细菌性阴道感染治疗前后的检测研究

解脲支原体 (Ureaplasmaurealyticum ,简称Uu)是引起不孕症的重要病因 ,临床治疗疗程较长 ,在治疗后期经常发现患者并发细菌性阴道病 (bacterialvaginosis,简称BV) ,而治疗前阴道涂片没有检出线索细胞。为了探究是Uu感染治疗疗程长引起阴道菌群紊乱 ,导致BV ?还是早在治疗前患者阴道就潜伏BV的致病菌株 ?本研究对Uu感染的不孕患者作了治疗前后阴道线索细胞和阴道加德纳菌致病菌株基因 (G vag -DNA)检测和治疗前后的比较 ,现报道如下。1　对象和方法1 1　对象　 14 4例不孕患者系来自 2 0 0 2年 4月～ 2 0 0 3年 2月云南省第一人民医院…     

溶脲脲原体感染现况及耐药机制研究进展

支原体是最小的原核微生物,溶脲脲原体是引起泌尿生殖道感染最为常见的病原体之一。该文收集参考国内外相关文献对溶脲脲原体对大环内酯类、喹诺酮类、四环素类抗生素耐药机制的研究取得的进展进行综述。     

Ureaplasma urealyticum and mycoplasma hominis in women with systemic lupus erythematosus

Objective. To determine the prevalence of genitourinary mycoplasma infection in women with systemic lupus erythematosus (SLE). Methods. Urine specimens from 49 patients with SLE and 22 patients with chronic fatigue syndrome (CFS) were cultured for mycoplasma. Patient records were reviewed for medical history and SLE disease activity. Results. Sixty-three percent of the SLE patients were culture positive, compared with 4.5% of the CFS patients (P < 0.001). Neither corticosteroid treatment, SLE activity, nor age accounted for this difference. Conclusion. Genitourinary mycoplasma colonization occurs significantly more frequently in SLE than in CFS.     

Ureaplasma urealyticum and Mycoplasma hominis in women with systemic lupus erythematosus.

OBJECTIVE. To determine the prevalence of genitourinary mycoplasma infection in women with systemic lupus erythematosus (SLE). METHODS. Urine specimens from 49 patients with SLE and 22 patients with chronic fatigue syndrome (CFS) were cultured for mycoplasma. Patient records were reviewed for medical history and SLE disease activity. RESULTS. Sixty-three percent of the SLE patients were culture positive, compared with 4.5% of the CFS patients (P less than 0.001). Neither corticosteroid treatment, SLE activity, nor age accounted for this difference. CONCLUSION. Genitourinary mycoplasma colonization occurs significantly more frequently in SLE than in CFS.     

Serologic studies of human genital mycoplasmas: distribution of titers of mycoplasmacidal antibody to Ureaplasma urealyticum and Mycoplasma hominis in pregnant women

Titers of mycoplasmacidal antibody to the human genital mycoplasmas Ureaplasma urealyticum and Mycoplasma hominis were determined using genital isolates from pregnant patients as antigens and comparing these isolates with the 11 prototypic reference strains for U. urealyticum and the seven reference strains for M. hominis. Virtually all titers that were detected with use of the patient's own isolates were detected by the 11 reference strains of U. urealyticum and by the seven reference strains of M. hominis. Serologic surveys of pregnant women who harbored either or both mycoplasmas in vaginal cultures indicated that antibody to M. hominis was found more commonly than antibody to U. urealyticum. It was demonstrated that significant postpartum rises in titers of antibody to M. hominis were correlated with the presence of these mycoplasmas in genital cultures. Postpartum rises in titer of antibody were particularly likely to occur in women with low titers of mycoplasmacidal antibody in serum at the time of delivery. Approximately 88% of the women who were colonized with M. hominis showed significant changes in titer of antibody to M. hominis throughout an apparently normal pregnancy; only 40% of the women who were colonized with U. urealyticum showed such changes in titers to U. urealyticum. Statistical analysis showed that mean log titers of antibody to both mycoplasmas at the first prenatal visit were significantly associated with the number of pregnancies experienced by these women.