针刺足阳明经穴对健康人血浆胃动素及胃泌素含量的影响

目的：进一步探讨足阳明经与胃运动内在联系的物质基础,方法：对50例健康人针刺前后各采用5ml,采用放射免疫分析法测量血浆胃动素,胃泌素的含量,结果：针刺四白,足三里穴后,血浆胃动素的含量明显升高,与针前相比差异有显著意义（P＜0．05）,针刺四社对照点后血中胃泌素明显升高,与针刺前相比差异有显著性意义（P＜0．05）,结论：针刺足阳明经穴位可使血浆胃动素含量上升,与胃运动增强呈平行关系,提示胃运动增强有胃动素的参与。     

针刺足阳明经穴对兔胃粘膜损伤前后胃运动功能的影响

目的 :通过针刺足阳明经穴对兔胃粘膜损伤前后胃运动的观察 ,进一步探讨足阳明经与胃的相关规律。方法 :对 40只大耳白兔运用无水乙醇灌胃 ,造成胃粘膜损伤模型 ,然后观察针刺对胃粘膜损伤前后胃运动功能的影响。结果 :胃粘膜损伤造模前后 ,胃运动振幅指数百分率比较差异有显著性意义 ,造模后振幅指数显著下降 ,与造模前比较 P <0 .0 1,针刺四白、足三里穴能增强胃运动 ,针刺后比刺前胃运动振幅指数显著升高 (P <0 .0 1) ,尤以四白穴最为显著 ,且在胃粘膜损伤后 ,同样能增强胃的运动。结论 :胃粘膜损伤后 ,胃运动振幅指数显著下降 ,针刺足阳明经穴后可使胃运动振幅指数上升 ,提示足阳明经与胃具有相关性。     

针刺足阳明经穴对大鼠胃运动及脑肠肽的影响

目的：探讨针刺足阳明经穴对胃运动的调整作用与脑肠肽的关系。方法：以乙醇灌胃造成大鼠胃粘膜损伤模型,气囊法测量胃运动频率和波幅的变化率,采用放免分析法（RIA）检测大鼠胃窦及延髓内P物质（SP）、胃动素（MTL）及胃泌素（GAS）含量。结果：模型组胃运动频率和波幅呈抑制状态,针刺四白、天枢、足三里穴可促进胃运动恢复,与此同时胃窦、延髓内SP、MTL、GAS含量出现相应变化。三穴比较：四白主要影响胃窦MTL含量,天枢主要影响胃窦SP及延髓GAS含量,足三里穴对SP、MTL及GAS均有影响。结论：推测针刺足阳明经对胃运动的调整作用有脑肠肽参与,但上述三穴对胃运动的影响所涉及的脑肠肽不完全一致。     

针刺足三里对功能性消化不良患者血浆胃肠激素的影响

目的：探讨针刺足三里对功能性消化不良（FD）患者血浆胃肠激素的影响,以及胃肠激素在FD发病中的可能作用及其临床意义。方法：对20例FD患者针刺前后各抽取血液5ml,采用放免分析法测定患者空腹血浆胃动素（MLT）、胃泌素（GAS）、生长抑素（SS）含量。结果：针刺足三里后,血浆MLT含量明显上升,与针前比较差异有非常显著性意义（P＜0．01）;SS含量明显降低,与针前比较差异有显著性意义（P＜0．05）;FD患者基础血浆MLT明显低于健康者。结论：针刺足三里对FD患者胃肠激素具有良好的调整作用,并提示胃肠激素可能参与了FD的发病或病理生理过程。     

针刺四白及内庭穴对胃蠕动功能的影响

目的：观察针刺足阳明经上下对应的四白、内庭穴对胃蠕动功能的影响,进一步探讨足阳明经与胃的相关规律。方法：选择１２ 例正常人及３ 例胃疾病患者为观察对象,对其四白、内庭及左右旁开对照点进行针刺,并以Ｂ超显像观察针刺前后胃蠕动波频率、电子标尺测蠕动波幅,同时摄片记录。结果：针刺足阳明经的四白、内庭穴对胃蠕动功能有特异性影响。结论：足阳明经四白、内庭穴对其相应的内脏有特异性,为临床调整消化系统功能的有效穴     

糖尿病大鼠血浆和组织中胃泌素、胃动素、胰高糖素的变化及意义

目的探讨糖尿病大鼠血浆和胃组织中胃肠激素的变化.方法选择雄性SD大鼠26只,用四氧嘧啶建立糖尿病模型,4周后取血浆和胃窦组织中测定各鼠胃泌素、胃动素、胰高糖素水平.结果与正常组比,糖尿病大鼠血浆中胃泌素和胃动素水平低下(P＜0.05);胰高糖素水平增高(P＜0.05);胃组织中胃动素水平低下(P＜0.05).结论糖尿病大鼠血浆中胃泌素、胃动素水平低下,胰高糖素水平增高,胃组织中胃动素水平低下.     

针刺四白及内庭穴对胃蠕动功能的影响

目的：观察针刺足阳明经上下对应的四白、内庭穴对胃蠕动功能的影响，进一步探讨足阳明经与胃的相关规律。方法：选择１２例正常人及３例胃疾病患者为观察对象，对其四白、内庭及左右旁开对照点进行针刺，并以Ｂ超显像观察针刺前后胃蠕动波频率、电子标尺测蠕动波幅，同时摄片记录。结果：针刺足阳明经的四白、内庭穴对胃蠕动功能有特异性影响。结论：足四明经四白、内庭穴对其相应的内脏有特异性，为临床调整消化系统功能的有效穴。     

甲状腺胃动素对大鼠胃运动的影响及下丘脑室旁核的调控作用

目的 探讨甲状腺胃动素对胃窦移行性复合运动(MMC)的影响及下丘脑室旁核(PVN)的调控作用.方法 采用荧光免疫组化技术,观察大鼠和人甲状腺、人甲状腺髓样癌(TT)细胞胃动素的表达,以及胃动素在人甲状腺髓样癌(MTC)组织中的定位和定性表达；采用在体胃运动和放射免疫方法,分别观察甲状腺切除前后、电刺激下丘脑PVN后大鼠胃窦MMCⅢ期运动变化及血浆游离三碘甲腺原氨酸(FT3)、游离甲状腺素(FT4)和胃动素含量的改变,以及甲状腺胃动素表达的改变；采用免疫组化法,观察甲状腺切除对PVN内c-Fos表达的影响.结果 大鼠和人甲状腺组织以及TT细胞均可见胃动素免疫阳性细胞的表达,在MTC组织中胃动素与降钙素共表达,即胃动素表达于甲状腺C细胞；甲状腺切除2d、4d后,大鼠血浆胃动素含量显著减少(2d,P＜0.01；4d,P＜0.05),胃窦MMCⅢ期样收缩活动明显减弱(2d,P＜0.01；4d,P＜0.05),PVN内c-Fos的表达显著增加(2h,P＜0.05).电刺激PVN可使大鼠血浆及甲状腺内胃动素含量显著增高(P＜0.05),胃窦MMCⅢ期样收缩明显增强(P＜0.05),该作用可部分被胃动素受体拮抗剂GM-109阻断(P＜0.05)；而电刺激甲状腺切除大鼠PVN,与甲状腺切除假手术组相比,其血浆胃动素含量显著降低(P＜0.05),胃窦MMCⅢ期样收缩明显减弱(P＜0.05).结论 甲状腺C细胞可分泌胃动素入血,且参与胃MMCⅢ期运动的调控,该活动可能受下丘脑PVN的调控.     

电针足阳明经穴对胃粘膜损伤大鼠胃粘膜血流量及生长抑素的影响

目的 :观察电针足阳明经“四白”、“天枢”、“足三里”穴对胃粘膜损伤大鼠胃窦和延髓内生长抑素(SS)含量的影响及与胃粘膜损伤、胃粘膜血流量的关系 ,以探讨经脉 -脏腑相关的物质基础。方法 :健康 SD大鼠 6 0只 ,随机分为正常对照组 (对照组 ) ,模型组 ,针刺四白组、天枢组及非穴点组共 6组 ,以乙醇灌胃造成胃粘膜损伤大鼠模型 ,观察电针大鼠足三里、天枢、四白穴对胃粘膜血流量 (氢气清除法 )的影响 ,用放免分析法检测大鼠胃窦及延髓 SS含量。结果 :胃粘膜血流量在胃粘膜损伤后明显降低 ,针刺四白、天枢、足三里及非穴点后 ,均有不同程度升高 ,尤以足三里和四白组升高明显 (P<0 .0 1) ;胃窦及延髓 SS模型组较对照组升高 (P<0 .0 5 ,<0 .0 1) ,而针刺四白、足三里组升高不明显 (P>0 .0 5 )。结论 :胃窦、延髓 SS含量变化与胃粘膜血流量的改变有一定关系 ,电针足阳明经四白、足三里穴可能通过对胃窦及延髓 SS含量的改变来影响胃粘膜血流量 ,促进胃粘膜损伤的修复。     

疏肝和胃冲剂治疗非溃疡性消化不良的临床疗效

[目的]探讨疏肝和胃冲剂治疗非溃疡性消化不良(NUD)的临床疗效.[方法]通过检测NUD患者经疏肝和胃冲剂、吗丁啉治疗前后血清胃泌素、胃动素及三维超声胃半排空时间,结合中医症状积分,综合评价2组药物的临床疗效.[结果]2组患者经药物治疗后症状总积分明显减少,血清胃泌素下降,胃动素升高,胃半排空时间缩短;与治疗前比较,差异有显著统计学意义(P＜0.01).疏肝和胃冲剂治疗后症状总积分及各项临床检测指标较吗丁啉治疗后改善更明显,差异有统计学意义(P＜0.01).[结论]疏肝和胃冲剂治疗NUD疗效可靠、无明显不良反应,具广泛的临床应用前景.     

Effect of electroacupuncture at Sibai on the gastric myoelectric activities of denervated rats

AIM: To explore the mechanism of the exciting effects of electro-acupuncture (EA) at Sibai on the gastric myoelectric activities. METHODS: A total of 32 rats were randomly divided into four groups. Through intraperitoneal injection with atropine (the anti-cholinergic agent by blockade of muscarinic receptors), hexamethonium (automatic nerve ganglion-blocking agent) and reserpine (anti-adrenergic agent by depleting the adrenergic nerve terminal of its norepinephrine store), effects of EA at Sibai on the gastric myoelectric activities of the denervated rats were observed. RESULTS: After intraperitoneal injection of atropine and hexamethonium, the average amplitude and ratio of period to time in the phase of high activity of gastric myoelectric slow wave, and the average numbers of the peaks of gastric myoelectric fast wave were significantly decreased (P<0.01, P<0.05, P<0.01), while after intraperitoneal injection of reserpine, the aforementioned three parameters were increased (P<0.01, P<0.05, P<0.01). EA at Sibai point partially relieved the inhibitory effect of atropine and hexamethonium on the gastric myoelectric activities in the rats (P<0.05 or P>0.05). CONCLUSION: Cholinergic and adrenergic nervous systems and autonomic nerve ganglion participate in the peripheral passage of the controlling effects of EA at Foot Yangming Channel on gastrointestinal tract.     

Effect of electroacupuncture at Sibai on the gastric myoelectric acitivities of denervated rats

INTRODUCTIONElectroacupuncture (EA) at certain points always exhibits marked effects (inhibitory or stimulatory) on organ activities. Our previous studies indicated that EA at the Sibai point exhibited exciting effects on gastric myoelectric activities. T…     

Effect of acupuncture at Foot-Yangming Meridian on gastric mucosal blood flow, gastric motility and brain-gut peptide

AIM： To observe the effect of acupuncture at Foot- Yangming Meridian on gastric mucosal blood flow （GMBF）, gastric motility and brain-gut peptide.
METHODS： Sixty SD rats were randomly divided into 6 groups： normal control group, model group （group with gastric mucosal damage, GMD）, Sibai group （with acupuncture at Sibai point ＋ GMD）, Tianshu group （with acupuncture at Tianshu point ＋ GMD）, Zusanli group （with acupuncture at Zusanli point ＋ GMD） and non-acupoint group （with acupuncture at non-acupoint ＋ GMD）. The GMD model group was induced by infusing pure alcohol into gastric cavity. H2 Gas Clearance Test （HGCT） was used to measure GMBF, the frequency and amplitude of gastric motility were measured by the method of aerocyst, the content of brain-gut peptide in sinus ventriculi and bulbus medullae were detected by radioimmunoassay.
RESULTS： Inhibitory effect of the frequency and amplitude of gastric motility were shown in model group, and the rates of frequency and amplitude changes were remarkably different from the normal control group （-19.41 ± 17.21 vs-4.71 ± 10.32, P 〈 0.05; -51.61 ± 29.02 vs 1.81 ± 14.12, P 〈 0.01）. In comparison with control group, the GMBF was 0.52 ± 0.161 mL vs 1.03 ± 0.255 mL per 100g tissue/min, P 〈 0.01, the content of motilin in sinus ventriculi and bulbus medullae was 63.04 ± 7.77 pg/mL vs 72.91 ± 8.42 pg/mL, P 〈 0.05 and 50.96 ± 8.77 pg/mL vs 60.76 ± 8.05 pg/mL, P 〈 0.05, but the content of somatostatin in sinus ventriculi and bulbus medullae was 179.85 ± 43.13 ng/g vs 90.54 ± 40.42 ng/g, P 〈 0.01 and 532.86 ± 122.58 ng/g vs 370.91 ± 76.29 ng/g, P 〈 0.05,respectively. In comparison with model group, the amplitude of gastric motility was 1.52 ± 20.13, -6.52 ± 23.31, 6.92 ± 25.21 vs -51.61 ± 29.02, P 〈 0.01 and GMBF was 0.694 ± 0.160 mL vs 0.893 ± 0.210 mL, 1.038 ± 0.301 mL vs 0.52 ± 0.161 mL per 100g tissue/rain, P 〈 0.01, respectively in Tianshu, Sibai and Zusanli groups, the content of moti     

小柴胡汤对正常大鼠胃肠激素影响的研究

[目的]观察小柴胡汤对大鼠血及胃组织胃动素（MOT）、促胃液素（Gas）和血管活性肠肽（VIP）的影响，探讨该方促胃肠动力作用的可能机制。[方法]健康SD大鼠30只随机分为小柴胡汤、莫沙比利和0．85％氯化钠3组，每组10只。用药后采用放射免疫方法检测大鼠血和胃组织中的MOT、Gas及VIP水平。[结果]小柴胡汤组可升高血和胃组织中MOT，与0．85％氯化钠组比较差异有统计学意义（P〈0．05），与莫沙比利组比较差异无统计学意义（P〉0．05）；小柴胡汤对血和胃组织中的Gas和VIP与0．85％氯化钠组比较P〉0．05。[结论]小柴胡汤的促胃肠动力作用可能与MOT密切相关。     

Sphincter of Oddi hypomotility and its relationship with duodenal-biliary reflux, plasma motilin and serum gastrin

AIM： To detect whether patients with a T tube after cholecystectomy and choledochotomy have duodenal-biliary reflux by measuring the radioactivity of Tc99m-labeled diethylene triamine penta-acetic acid （DTPA） in the bile and whether the patients with duodenal-biliary reflux have sphincter of Oddi hypomotility, by measuring the level of plasma and serum gastrin of the patients. Finally to if there is close relationship among sphincter of Oddi hypomotility, duodenal-biliary reflux and gastrointestinal peptides.
METHODS： Forty-five patients with a T tube after cholecystectomy and choledochotomy were divided into reflux group and control group. The level of plasma and serum gastrin of the patients and of 12 healthy volunteers were measured by radioimmunoassay. Thirty-four were selected randomly to undergo choledochoscope manometry. Sphincter of Oddi basal pressure （SOBP）, amplitude （SOCA）, frequency of contractions （SOF）, duration of contractions （SOD）, duodenal pressure （DP） and common bile duct pressure （CBDP） were scored and analyzed.
RESULTS. Sixteen （35.6%） patients were detected to have duodenal-biliary reflux. SOBP, SOCA and CBDP in the reflux group were much lower than the control group （t = 5.254, 3.438 and 3.527, P 〈 0.001）. SOD of the reflux group was shorter than the control group （t = 2.049, P 〈 0.05）. The level of serum gastrin and plasma motilin of the reflux group was much lower than the control group （t = -2.230 and -2.235, P 〈 0.05）. There was positive correlation between the level of plasma motilin and SOBP and between the level of serum gastrin and SOBP and CBDP.
CONCLUSION： About 35.9% of the patients with a T tube after cholecystectomy and choledochotomy have duodenal-biliary reflux. Most of them have sphincter of Oddi hypomotility and the decreased level of plasma motilin and serum gastrin. The disorder of gastroin-testinal hormone secretion may result in sphincter of Oddi dysfunction. There is a close relationship between sphincter     

治萎防变胶囊对气虚血瘀型CAG大鼠PGE2、GAS和血浆/胃组织MOT水平的影响

目的 探讨治萎防变胶囊对气虚血瘀型萎缩性胃炎(CAG)大鼠胃组织PGE2、血清胃泌素(GAS)和血浆/胃组织胃动素(MOT)水平的影响.方法 采用综合法复制CAG动物模型.施药治疗后分别对各组大鼠血清胃组织PGE2、血清GAS和血浆/胃组织MOT含量进行检测.结果 与空白组比较,模型组大鼠胃组织PGE2、血清GAS水平显著下降(P<0.01,P<0.05),血浆/胃组织MOT水平显著升高(P<0.05);与模型组比较,治萎防变胶囊可显著提高组织PGE2、血清GAS含量(P<0.05),显著降低血浆/胃组织MOT水平(P<0.05),且以治萎防变胶囊大剂量组作用显著.结论 治萎防变胶囊具有调节胃酸、胃黏膜黏液分泌,调整胃动力,增加胃黏膜血流量,改善微循环,营养和修复胃黏膜的作用.     

胃病患者血清胃液胃液素、胃动素和亮-脑啡肽的变化

目的 研究胃病患者血清胃液胃液素、胃动素和亮-脑啡肽的变化及临床意义。 方法 用放射免疫法测定胃病患者(胃癌25例、胃溃疡18例和萎缩性胃炎24例)血清胃液中胃液素、胃动素和亮-脑啡肽的含量。 结果 与正常对照和胃良性疾病比较,胃癌患者血清胃液胃液素、胃动素显著升高(P<0.01),血清亮-脑啡肽含量也升高(P<0.05)。 结论 胃癌患者血清胃液胃液素、胃动素和血清亮-脑啡肽可明显升高,对胃癌诊断有一定参考价值。     

布拉氏酵母菌联合三联疗法对中老年幽门螺杆菌阳性消化性溃疡患者胃泌素和血浆胃动素的影响

[目的]探讨布拉氏酵母菌联合三联疗法对中老年幽门螺杆菌阳性消化性溃疡患者胃泌素和血浆胃动素的影响.[方法]选择我院2013年1月～2014年3月收治的120例幽门螺杆菌阳性消化性溃疡的中老年患者,随机分为观察组和对照组,观察组采用布拉氏酵母菌联合三联疗法,对照组仅采用常用的三联疗法,对比分析2组患者的血清胃泌素和胃动素的水平.[结果]治疗后与对照组相比,观察组的有效率显著升高,差异有统计学意义（x2—11.791,P=0.000）;治疗前,观察组和对照组的胃泌素和胃动素水平差异无统计学意义;治疗后,2组的胃泌素水平在治疗1d、5d、10 d后均有显著下降（P＜0.01）,其中与对照组相比,观察组治疗1d、5d、10 d后均有显著下降,差异有统计学意义（t=10.461、10.312、9.874,P=0.001、0.000、0.001）;观察组和对照组的胃动素水平在治疗1d、5d、10 d后均有显著升高（P＜0.01）,其中与对照组相比,观察组也有显著上升,差异有统计学意义（t=12.554、16.361、18.754,P=0.000、0.000、0.001）.[结论]布拉氏酵母菌联合三联疗法对恢复中老年幽门螺杆菌阳性消化性溃疡患者的胃泌素和血浆胃动素的水平有更好的疗效.