了哥王化学成分研究

目的:对了哥王的茎皮进行化学成分研究。方法:硅胶及ODS柱色谱分离,光谱分析鉴定化合物结构。结果:分离了6个化合物,分别鉴定为西瑞香素-7-O-β-D-葡萄糖苷(1),槲皮苷(2),大黄素甲醚(3),山柰酚-3-芸香糖苷(4),芫花苷(5),伞形香青酰胺(6)。结论:6个化合物均为首次从该植物中分离得到。     

了哥王化学成分研究

目的研究了哥王主要毒性部位的化学成分。方法利用各种层析分离技术对了哥王主要毒性部位乙酸乙酯部位进行分离纯化,经理化常数和波谱解析确定结构。结果从中分离鉴定了16个化合物,分别为:十六烷酸(Ⅰ)、硬脂酸甲酯(Ⅱ)、大黄酚(Ⅲ)、大黄素甲醚(Ⅳ)、阿魏酸(Ⅴ)、邻苯二甲酸二丁酯(Ⅵ)、β-谷甾醇(Ⅶ)、伞形花内酯(Ⅷ)、西瑞香素(Ⅸ)、芫花素(Ⅹ)、没食子酸(Ⅺ)、西瑞香素-7-O-β-D-葡萄糖苷(Ⅻ)、β-胡萝卜苷(ⅩⅢ)、木犀草素(ⅩⅣ)、山萘酚(ⅩⅤ)、槲皮苷(ⅩⅥ)。结论化合物Ⅱ、Ⅲ、Ⅴ、Ⅺ和ⅩⅣ为首次从该植物中分离得到。     

了哥王芳香类化学成分研究

目的:对了哥王的化学成分进行研究.方法:采用硅胶柱层析法从了哥王乙醇提取物的乙酸乙酯萃取部位中分离化合物,采用光谱分析和文献对照的方法,确定它们的结构.结果:共分得4个化合物,分别为:邻苯二甲酸二丁酯(Ⅰ)、对羟基苯甲酸甲酯(Ⅱ),2,4,6-三羟基苯甲酸甲酯(Ⅲ)和芫花素(Ⅳ).结论:化合物Ⅰ、Ⅱ、Ⅲ为首次从该植物中分离得到,亦首次从瑞香科植物中得到.     

了哥王的化学成分和药理作用研究进展

通过查阅、整理国内外有关了哥王的文献资料,对其化学成分和药理毒副作用进行归纳总结。化学成分主要包括香豆素类、黄酮类和木脂素类等,且多具生理活性;药理作用主要有抑菌、抗病毒、抗炎镇痛、抗肿瘤作用等;了哥王作为一种有毒中药,其毒副作用方面也应引起足够重视,服用剂量不宜过大,孕妇忌用。近年来报道证实了哥王有显著的抗肿瘤活性,使该药成为研究热点。建议深入系统地开展其化学成分研究。     

了哥王抗肿瘤活性部位筛选

目的:研究了哥王95%、75%、50%乙醇提取物、水提物及95%乙醇提取物的石油醚、氯仿、乙酸乙酯、正丁醇萃取部位对肿瘤细胞的增殖抑制作用,确定了哥王抗肿瘤活性部位。方法:采用四甲基偶氮唑盐(MTT)比色法考察了哥王不同提取物及95%乙醇提取物的不同溶剂萃取部位对Hela、SGC-7901、Bel-7402细胞的抗肿瘤活性。结果:了哥王95%乙醇提物对Hela、SGC-7901细胞有良好的抗肿瘤活性,其石油醚、氯仿、乙酸乙酯萃取部位对Hela、SGC-7901细胞均有不同程度的抗肿瘤活性。结论:石油醚、氯仿、乙酸乙酯萃取部位有较强的抗肿瘤活性,初步确定为了哥王抗肿瘤活性部位。     

辨证加了哥王治疗肝硬化24例

<正>笔者自1987年以来在中医辨证施治的基础上加了哥王治疗肝硬化24例,效果满意。现小结如下。 1 临床资料 24例均为门诊病人,其中男19例,女5例;年龄最小23岁,最大66岁,平均46岁;病程最长6年,最短1年,平均3.5年;按病期分:早期肝硬化17例,肝硬化腹水7例;按病因分类:肝炎后肝硬化18例,酒精性肝硬化2例,血吸虫性肝硬化1例,心源性肝硬化1例,原因不明肝硬化2例;并发腹水感染3例,肝昏迷1例,上消化道出血2例,肝癌1例。全部病例均按《临床疾病诊断依据治愈好转标准》确诊。     

了哥王提取物及其不同提取部位的急性毒性研究

目的观察了哥王提取物及其不同提取部位的急性毒性大小。方法小鼠一次灌胃给予了哥王提取物及其不同提取部位后,观察14 d内各受试样品小鼠的反应情况及死亡情况。记录各样品不同剂量组小鼠死亡只数,用Bliss法计算出该样品的半数致死量(LD50);无小鼠死亡的样品,则测定其最大给药量。结果了哥王提取物的LD50为3.276 g/kg;了哥王石油醚部位的LD50为1.691 g/kg;了哥王醋酸乙酯部位的LD50为1.948 g/kg;了哥王正丁醇部位的最大给药量为24.364 g/kg;了哥王水部位的最大给药量为:32.788 g/kg。结论了哥王提取物的毒性随脂溶性的增大而增大,可以推断其毒性成分主要为脂溶性成分。     

了哥王叶子化学成分研究(Ⅰ)

对瑞香科荛花属植物了哥王[Wikstroemia indica(L.)C.A.Mey]的叶子进行化学成分研究。方法:用甲醇提取及柱色谱等方法进行分离,薄层色谱跟踪检测,波谱法鉴定结构。结果:从该植物叶子中共分离鉴定出6个化合物,分别是西瑞香素(1)、异西瑞香素B(2)、杨梅素(3)、槲皮素(4)、山奈酚(5)、芫花素(6)。结论:首次报道了哥王叶子的化学成分。     

了哥王药材HPLC指纹图谱研究

目的：建立了哥王的高效液相色谱（HPLC）指纹图谱。方法：采用Alhima C18（4．6mm×250mm,5μm）色谱柱;流动相为0．1％磷酸水-乙腈（梯度洗脱）;流速为1ml·min-1;检测波长为320nm;柱温为25℃。结果：建立了哥王HPLC指纹图谱分析的研究方法。结论：了哥王药材HPLC指纹图谱分析方法操作简单,精密度高,重复性好,可用于了哥王的质量控制。     

了哥王片治疗软组织损伤67例

笔者自2004年1月～12月用了哥王片口服治疗急慢性软组织损伤和四肢骨折67例,现将临床观察结果报告如下.     

(+)-Nortrachelogenin, a new pharmacologically active lignan from Wikstroemia indica.

A new lignan, (+)-nortrachelogenin (I), and a known compound, daphnoretin were isolated from Wikstroemia indica C. A. Meyer (Thymelaeaceae). The structure of (+)-nortrachelogenin was established as 8(R), 8'(R)-4,4',8'-trihydroxy-3,3'-dimethoxylignan-olid(9, 9') on the basis of spectroscopic evidence and comparison with its enantiomer, (-)-nortrachelogenin. (+)-Nortrachelogenin (I) showed effects on the central nervous system producing depression in rabbits.     

Antiviral activity of daphnoretin isolated from Wikstroemia indica

The ethanol extract of Wikstroemia indica was fractionated with organic solvents of different polarities, and various fractions were screened for their antiviral activity against respiratory syncytial virus (RSV) using a cytopathic effect (CPE) reduction assay. The ethyl acetate fraction was most active against RSV with 50% inhibition concentration (IC₅₀) value < 3.9 μg/mL and a selectivity index (SI) > 64.1. Further isolation and purification of the fraction led to a purified compound, daphnoretin. Daphnoretin was tested for its anti‐RSV activity using a plaque reduction assay and found active against RSV, with an IC₅₀ value of 5.87 μg/mL and SI value of 28.17. The mode of antiviral action study revealed that daphnoretin could slightly inhibit the early events of the viral infection but its effect was mainly on the later phase of the replication cycle. Copyright © 2009 John Wiley & Sons, Ltd.     

Indicanine A, a new 3-phenylcoumarin from root bark of Erythrina indica

A new 3-phenylcoumarin, indicanine A (1), has been isolated from the root bark of the African medicinal plant Erythrina indica, together with three known compounds, robustic acid (2), daidzein, and 8-prenyldaidzein. The structure of the new compound was characterized, as 4-hydroxy-5-methoxy-3-(4'-methoxyphenyl)-2" -(1-methylethenyl)dihydrofurano[4",5":6,7]coumarin by means of extensive spectroscopic analyses. The compounds were found to be devoid of in vitro antibacterial activity.     

羽叶丁香茎皮中1个新木脂素成分

通过色谱方法从羽叶丁香的茎皮中分到11个化学成分,经质谱、核磁共振谱及与文献数据相比鉴定其结构。其中化合物1为新木脂素,命名为反式羽叶丁香素A(Z-pinnatifolin A),10个已知物分别鉴定为pinnatifolin A(2),乙基松柏苷ethylconiferin(3),甲基松柏苷(4),丁香素(5),β,3,4-trihydroxyphenethyl alcohol(6),(S)-(+)-2-(3,4-dihydroxyphenyl)-2-ethoxyleth-anol(7),3,4-dihydroxy-β-methoxyphenethyl alcohol(8),3,4,5-三羟基苯乙醇(9),红景天苷(10),羟基酪醇(11),其中3,4,8~11为丁香属内首次报道,5~7为该种首次报道。化合物1对神经小胶质细胞BV-2的NO生成显示一定的抑制活性。该研究为表征山沉香抗心肌缺血的药效物质提供了一定的参考。     

Immunomodulatory activity of an aqueous extract of Azadirachta indica stem bark

The interference of an aqueous extract of the stem bark of Azadirachta indica with different parts of the human immune system was investigated. The extract showed strong anticomplementary effects which were dose-and time-dependent and most pronounced in the classical complement pathway assay. Moreover, a dose-dependent decrease in the chemiluminescence of polymorphonuclear leukocytes was observed and a dose-dependent increase in the production of migration inhibition factor by lymphocytes.     

傣药翅荚决明树皮中1个新呋喃-2-甲酸类化合物

对傣药翅荚决明Cassia alata树皮的化学成分进行研究。运用硅胶、凝胶、MCI-gel树脂及RP-HPLC等多种色谱技术从翅荚决明树皮95%乙醇提取物中分离鉴定了1个新呋喃-2-甲酸类化合物,鉴定为5-[3-(羟甲基)-4,5-二甲氧基苯]-3-甲基呋喃-2-甲酸(1)。生物活性测试中,其对NB4,A549,SHSY5Y,PC3和MCF7的IC50分别为2.5,1.2,2.2,3.6,1.9μmol·L~(-1)。化合物1为新化合物,并且表现出一定的细胞毒活性。     

Three new resveratrol oligomers from the stem bark of Vatica pauciflora

Three new stilbene oligomers (1-3) were isolated from the stem bark of Vatica pauciflora. The structures of a resveratrol heptamer (pauciflorol D) (1), a resveratrol dimer (pauciflorol E) (2), and an indanone derivative (pauciflorol F) (3) were elucidated by means of spectroscopic data interpretation, especially HMBC and NOESY NMR experiments.     

Four new trisnorlanostene-type triterpenoids from the stem bark of Pinus luchuensis

Four new 25,26,27-trisnorlanostene-type triterpenoids were isolated from the stem bark of Pinus luchuensis, together with five known compounds. These new compounds were characterized as 25,26, 27-trisnor-3 alpha-hydroxy-lanost-9(11)-en-24-oic acid (1), 25,26, 27-trisnor-3 alpha-methoxy-lanost-9(11)-en-24-oic acid (2), 25,26, 27-trisnor-3 beta-methoxy-lanost-9(11)-en-24-oic acid (3), and 25,26, 27-trisnor-3-oxo-lanost-9(11)-en-24-oic acid (4) on the basis of MS and NMR evidence.