CpG-ODN对树突状细胞的分化成熟的影响

目的：探讨ＣｐＧ－ＯＤＮ对小鼠骨髓来源的树突状细胞（ｂｏｎｅ ｍａｒｒｏｗ－ｄｅｒｉｖｅｄ ｄｅｎｄｒｉｔｉｃ ｃｅｌｌｓ,ＢＭＤＣ）分化成熟的影响。方法：利用合成的含非甲基化ＣｐＧ基序的寡核苷酸（ＧｐＧ ｍｏｔｉｆ ｃｏｎｔａｉｎｉｎｇｏｌｉｇｏｎｕｃｌｅｏｔｉｄｅｓ,ＣｐＧ－ＯＤＮ）,通过酶切鉴定ＤＮＡ制品ＣｐＧ基序甲基化程序,ＦＡＣＳ分析ＤＣ表型和内吞作用的变化,ＥＬＩＳＡ检测ＤＣ培养上清     

结肠癌细胞中Ese-3对树突状细胞分化成熟的影响

目的:利用体外共培养实验,观察结肠癌细胞SW480中上皮特异性转录因子Ese-3表达水平的改变对树突状细胞（dendritic cell,DC）分化成熟的影响。方法:利用慢病毒系统获得过表达Ese-3的结肠癌细胞SW480（SW480 Ese-3）及其对照细胞株（SW480 NC）。利用磁珠分选方式,从人外周血单个核细胞（PBMC）中获得CD14+细胞,经rhGM-CSF、rhIL-4刺激获得不成熟的DC（iDC）。通过Transwell小室将SW480 Ese-3和SW480 NC细胞分别与iDC进行间接共培养。流式细胞术检测共培养后的DC细胞中HLA-DR、CD14、CD83、CD86 的表达。ELISA检测细胞培养上清中IL-12p70的水平。结果:SW480 Ese-3/DC共培养组DC细胞表面标志物HLA-DR、CD83、CD86水平,较SW480 NC/DC共培养组升高,CD14水平降低;SW480 Ese-3/DC共培养组上清中IL-12p70水平较对照组升高。结论:在体外共培养实验中,SW480细胞过表达Ese-3可促进DC细胞分化成熟。     

胶质瘤细胞miR-153上调对树突状细胞分化成熟及Nrf2表达的影响

目的:探讨胶质瘤细胞miR-153上调对树突状细胞分化成熟及Nrf2表达的影响。方法:体外培养小鼠胶质瘤细胞系GL261,随机分为对照组、miR-153 mimics阴性对照组、miR-153 mimics组,以miR-153 mimics阴性对照、miR-153 mimics分别处理GL261,以实时荧光定量PCR（qRT-PCR）检测各组GL261细胞miR-153、VEGF-A及IL-10 mRNA水平;以蛋白免疫印迹法检测各组GL261细胞VEGF-A、IL-10、Nrf2蛋白表达;以流式细胞仪检测DC2.4细胞表面共刺激分子MHC-II、CD80、CD86、CD40表达水平;将小鼠T淋巴细胞系与上述各组DC2.4细胞共培养,以CCK-8检测T细胞增殖情况。结果:与对照组相比,miR-153 mimics组GL261细胞miR-153、VEGF-A及IL-10 mRNA水平,VEGF-A及IL-10蛋白表达明显降低（P＜0.05）。DC2.4表面共刺激分子MHC-II、CD80、CD86及CD40表达水平,DC2.4细胞Nrf2蛋白表达,T细胞活力明显升高（P＜0.05）;miR-153 mimics阴性对照组细胞各指标无明显变化（P＞0.05）。结论:上调miR-153可抑制胶质瘤细胞分泌免疫抑制性因子VEGF-A、IL-10,促进树突状细胞分化成熟并上调其Nrf2蛋白表达。     

HSP70-PC肽复合物与树突状细胞成熟关系的实验研究

目的探讨HSP70-PC肽复合物与人外周血来源的树突状细胞DC成熟之间的关系。方法采用GM—CSF、IL-4刺激培养人外周血单个核细胞，增殖产生大量DCS；从肺癌细胞中获取肽复合物，通过细胞因子检测试剂盒进行检测。结果发现HSP70-PC肽复合物修饰的DDC大量分泌IL-12、TNF-α等细胞因子。结论HSP70-PC肽复合物可诱导DC成熟，体外产生特异性反应。     

小鼠骨髓树突状细胞对胃癌细胞杀伤作用的研究

目的研究小鼠骨髓树突状细胞的制备及其对胃癌细胞的杀伤作用。方法添加细胞因子培养扩增小鼠骨髓源树突状细胞;流式细胞仪检测DC表面标志物CD11c、CD86的表达;MTT法测定DC对胃癌细胞的杀伤作用。结果培养第7天出现扩增之骨髓源DC,加入TNF-α24 h后,成熟骨髓源DC形成。每只615小鼠平均能分离出3×10^7个骨髓细胞,一只小鼠的骨髓单个核细胞DC的产量约为7×10^6个。加入TNF-α48 h后,DC表面标志物CD86阳性表达率较加入TNF-α前增高;CD11c阳性表达率加入TNF–α前后无差别。成熟DC与615小鼠胃癌细胞共孵育24 h、48 h,效靶比分别为10∶1、20∶1、30∶1时,DC对胃癌细胞的杀伤活性随效靶比的增大而增强。结论提取纯化小鼠骨髓细胞,经细胞因子GM-CSF、IL-4、TNF-α的刺激诱导可产生成熟DC,成熟DC对胃癌细胞有较强的杀伤作用,其杀伤活性随效靶比的增大而增强,随共孵育时间的延长杀伤活性有增强的倾向。     

小鼠骨髓树突状细胞体外培养扩增与生物学特性研究

目的：建立一种简便高效的体外扩增培养小鼠骨髓树突状细胞（bonemarrowdendriticcell,BMDC）的方法,为树突状细胞（dendl’iticcells,DCs）的理论研究与临床应用提供实验工具。方法：联合应用重组小鼠粒细胞一巨噬细胞集落刺激网子（rmGM—CSF）、蘑纽小鼠白介素（rmlL-4）诱导培养小鼠骨髓单个核细胞,终末加入重组小鼠肿瘤坏死因子（rmTNF—α）,从形态学表型及功能方面进行检测。结果：培养3天后可见大量贴壁细胞及细胞集落形成,第5天,可见典型的树突状突起,光镜下动态观察树突状细胞DC的形态变化,流式细胞仪检测各组DC加入TNF—α前后表面标志物CD11C、CD86的衷达,加入TNF—α后CD86的阳性表达率明显增高,CD11C的阳性表达率变化无统计学意义。结论：此方法能在体外诱导和扩增出大量骨髓源性DC,为抗肿瘤疫苗研究及临床应用奠定基础。     

树突状细胞对LAK细胞抗肿瘤活性的影响

树突状细胞（Ｄｅｎｄｒｉｔｉｃｃｅｌ，ＤＣ）是一种高效的抗原呈递细胞，它在抗肿瘤免疫方面的作用已受到人们的关注。本文在成功分离获得人外周血ＤＣ的基础上，探讨不同浓度的ＤＣ对ＬＡＫ细胞抗肿瘤活性的影响，旨在探索ＤＣ提高ＬＡＫ细胞活性的最适剂量，为进一步...     

粒-巨噬细胞集落刺激因子剂量对小鼠骨髓源性树突状细胞成熟状态的影响

 目的　观察不同剂量粒-巨噬细胞集落刺激因子（GM-CSF）对小鼠树突状细胞（DC）形态、纯度、成熟程度的影响,为不同用途的DC疫苗制备选择合适的GM-CSF质量浓度提供理论依据。方法　用低剂量（5 ng／ml）、常规剂量（20 ng／ml）、高剂量（50 ng／ml）GM-CSF联合重组小鼠白细胞介素-4（mIL-4）诱导小鼠骨髓细胞体外分化为DC,流式细胞术测定CD11c、CD80和CD86表达率。结果　低剂量GM-CSF组诱导的DC不具有典型的DC形态,低表达CD11c、CD80和CD86。常规剂量GM-CSF组诱导的DC高表达CD11c、CD80和CD86,具有更强的刺激同种异体淋巴细胞增殖能力。高剂量GM-CSF组CD11c、CD80和CD86表达率最高,并具有最强的刺激同种异体淋巴细胞增殖的能力。结论　GM-CSF剂量影响诱导分化的DC成熟程度。低剂量的GM-CSF诱导的DC为不成熟的DC,常规剂量和高剂量GM-CSF诱导分化的DC为细胞表型和功能成熟的DC。高剂量GM-CSF诱导的DC细胞表型和功能最成熟。     

人脐带血树突状细胞对LAK细胞杀伤活性的影响

目的研究人脐带血树突状细胞(DC)对LAK细胞杀伤活性的影响.方法从人脐带血中分离出DC,以不同浓度与LAK细胞共育,检测其杀伤活性变化.结果加入低于1×105/mlDC的LAK杀伤活性显著增强,高于此浓度则对LAK活性呈不显著或负面影响.结论该结果提示脐血DC对LAK细胞杀伤活性可能具双向调节作用.     

小鼠红白血病细胞来源的树突状细胞对特异性CTL的体内外诱 …

目的 探讨白细胞介素－１２（ＩＬ－１２）诱导小鼠红白血病细胞产生的树突状细胞,对白血病特异性ＣＴＬ细胞的诱导作用,及体内免疫后对抗肿瘤免疫应答的诱导效果。方法：采用４小时＾５１Ｃｒ释放法,检测ＣＴＬ杀伤活性;观察小鼠红白细胞病细胞来源的树突状细胞体内免疫治疗后对肿瘤的抑制作用,采用了ＨＥ染色和电镜等技术,分析肿瘤组织的病理学变化。结果：ＩＬ－１２诱导ＦＢＬ－３细胞产生的ＤＣ,在体外可诱导出ＦＢＬ－     

Modulation of viability and maturation of human monocyte-derived dendritic cells by oncolytic adenoviruses

Adenoviral oncolysis is a promising new modality for treatment of cancer based on selective viral replication in tumor cells. However, tumor cell killing by adenoviral oncolysis needs to be improved to achieve therapeutic benefit in the clinic. Towards this end, the activation of anti-tumor immunity by adenoviral oncolysis might constitute a potent mechanism for systemic killing of uninfected tumor cells, thereby effectively complementing direct tumor cell killing by the virus. Knowledge of anti-tumor immune induction by adenoviral oncolysis, however, is lacking mostly due to species-specificity of adenovirus replication, which has hampered studies of human oncolytic adenoviruses in animals. We suggest the analysis of interactions of oncolytic adenoviruses with human immune cells as rational basis for the implementation of adenoviral oncolysis-induced anti-tumor immune activation. The goal of our study was to investigate how oncolytic adenoviruses affect human dendritic cells (DCs), key regulators of innate and adoptive immunity that are widely investigated as tumor vaccines. We report that melanoma-directed oncolytic adenoviruses, like replication-deficient adenoviruses but unlike adenoviruses with unrestricted replication potential, are not toxic to monocyte-derived immature DCs and do not block DC maturation by external stimuli. Of note, this is in contrast to reports for other viruses/viral vectors and represents a prerequisite for anti-tumor immune activation by adenoviral oncolysis. Furthermore, we show that these oncolytic adenoviruses alone do not or only partially induce DC maturation. Thus additional signals are required for optimal immune activation. These could be delivered, for example, by inserting immunoregulatory transgenes into the oncolytic adenovirus genome.     

香菇多糖促进急性髓细胞性白血病患者DC的成熟并增强其功能

目的： 研究香菇多糖（lentinan, LNT）对急性髓细胞性白血病（acute myeloid leukemia, AML）患者树突状细胞（dendritic cell,DC）成熟及功能的影响,探索白血病免疫治疗的新途径。 方法： 分离AML完全缓解期患者（AML-complete remission,AML-CR）的骨髓单个核细胞（bone marrow mononuclear cell,BMC）,用GM-CSF和IL-4诱导7 d培养DC,然后分组：LPS阳性对照组、LNT实验组和对照组。培养48 h后以瑞氏－吉姆萨(Wright-Giemsa)染色观察各组DC形态,流式细胞术检测各组DC表面分子CD80、CD83、CD86、CD1a和HLA-DR的表达,ELISA法检测各组DC上清液中IL-12的水平。免疫磁珠法分离AML-CR患者经LNT治疗后的外周血DC,ELISA法检测治疗后DC上清液中IL-12的水平。 结果： 体外实验中,LNT处理后DC呈典型树突状形态;其表面分子CD80、CD83、CD86、CD1a及HLA-DR的表达上调（P<0.05）;DC分泌IL-12较阴性对照组显著增高（P<0.05）,且呈LNT浓度依赖性。患者体内试验结果显示,LNT治疗后AML患者的DC上清液中IL-12分泌水平较治疗前显著提高（P<0.05）。 结论： LNT在体内外均能促进AML患者的DC成熟和增强DC功能。     

Efficient antitumor immunity derived from maturation of dendritic cells that had phagocytosed apoptotic/necrotic tumor cells

Dendritic cells (DCs) that acquired antigen from apoptotic tumor cells are able to induce major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes and antitumor immunity. In the present study, we investigated the efficiency of antitumor immunity derived from DCs that had phagocytosed apoptotic/necrotic BL6-10 melanoma cells compared with that of DCs pulsed with the tumor mTRP2 peptide. Our data showed that phagocytosis of apoptotic/necrotic tumor cells resulted in maturation of DCs with up-regulated expression of proinflammatory cytokines [interleukin (IL)-1beta, IL-6, tumor necrosis factor-alpha, interferon-gamma and granulocyte-macrophage colony-stimulating factor], chemokines (MIP-1alpha, MIP-1beta and MIP-2), the CC chemokine receptor CCR7 and the cell surface molecules (MHC class II, CD11b, CD40 and CD86), and down-regulated expression of the CC chemokine receptors CCR2 and CCR5. These mature DCs displayed enhanced migration toward the CC chemokine MIP-3beta in a chemotaxis assay in vitro and to the regional lymph nodes in an animal model in vivo. Our data also showed that vaccination with DCs that had phagocytosed apoptotic/necrotic BL6-10 cells was able to (i) more strongly stimulate allogeneic T-cell proliferation in vitro, (ii) induce an in vivo Th1-type immune response leading to more efficient tumor-specific cytotoxic CD8(+) T-cell-mediated immunity and (iii) eradicate lung metastases in all 6 vaccinated mice compared with mice vaccinated with DCs pulsed with the tumor mTRP2 peptide, in which lung metastases were reduced (mean number of 16 per mouse) but not completely eradicated. Therefore, DCs that had phagocytosed apoptotic/necrotic tumor cells appear to offer new strategies in DC cancer vaccines.     

The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells

OBJECTIVE This research was to induce dendritic cells （DCs） from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. METHODS Embryonic stem cells （ESCs） suspending cultured in petri dishes were induced to generate embryonic bodies （EBs）. Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GM- CSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. RESULTS Growing mature through exposure to lipopolysaccharide （LPS）, both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction （MLR）. CONCLUSION ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine.     

Human dendritic cells efficiently phagocytose adenoviral oncolysate but require additional stimulation to mature

Oncolytic adenoviruses are emerging agents for treatment of cancer by tumor-restricted virus infection and cell lysis. Clinical trials have shown that oncolytic adenoviruses are well tolerated in patients but also that their antitumor activity needs improvement. A promising strategy toward this end is to trigger systemic and prolonged antitumor immunity by adenoviral oncolysis. Antitumor immune activation depends in large part on antigen presentation and T cell activation by dendritic cells (DCs). Thus, it is likely that the interaction of lysed tumor cells with DCs is a key determinant of such "oncolytic vaccination." Our study reveals that human DCs effectively phagocytose melanoma cells at late stages of oncolytic adenovirus infection, when the cells die showing preferentially features of necrotic cell death. Maturation, migration toward CCL19 and T cell stimulatory capacity of DCs, crucial steps for immune induction, were, however, not induced by phagocytosis of oncolysate, but could be triggered by a cytokine maturation cocktail. Therefore, oncolytic adenoviruses and adenoviral oncolysate did not block DC maturation, which is in contrast to reports for other oncolytic viruses. These results represent a rationale for inserting immunostimulatory genes into oncolytic adenovirus genomes to assure critical DC maturation. Indeed, we report here that adenoviral transduction of melanoma cells with CD40L during oncolysis triggers the maturation of human DCs with T cell stimulatory capacity similar to DCs matured by cytokines. We conclude that triggering and shaping DC-induced antitumor immunity by oncolytic adenoviruses "armed" with immunostimulatory genes holds promise for improving the therapeutic outcome of viral oncolysis in patients.     

白细胞介素24对人树突状细胞活化和成熟的诱导作用

目的: 〖HT5"SS〗研究白细胞介素24（interleukin24,IL24）对人外周血单核细胞来源树突状细胞（dendritic cell, DC）表型及抗原提呈功能的影响。〖HT5W〗方法： 〖HT5"SS〗利用Western blot检测DC培养上清中IL24的分泌水平;利用半定量RTPCR方法分析不同条件下DC表达IL24受体及趋化因子受体的情况;通过流式细胞术分析检测IL24共培养后DC细胞表面CD80、CD86、MHCⅡ类分子的表达水平;采用体外混合淋巴细胞实验分析经IL24共培养对DC抗原提呈功能的影响。〖HT5W〗结果：〖HT5"SS〗 体外培养过程中,用LPS刺激DC可诱导其分泌IL24;同时,LPS还能上调DC表达IL24受体亚单位IL22R1。IL24作用于DC可上调DC细胞表面CD80、CD86及MHCⅡ类分子的表达,并增强DC对T细胞的抗原提呈功能。〖HT5W〗结论： 〖HT5"SS〗IL24这一新型的细胞因子在体外实验中能促进DC的表型成熟,增强DC的抗原提呈功能。     

流感疫苗促进髓系白血病骨髓细胞来源树突状细胞的功能

目的: 研究流感疫苗对髓系白血病骨髓源性树突状细胞（dendritic cells,DCs）功能的影响及其机制。方法：分离髓系白血病患者\[急性髓细胞白血病(acute myeloid leukemia, AML)19例, 慢性髓细胞白血病（chronic myeloid leukemia,CML) 8例\]骨髓单个核细胞（mononuclear cell,MNC）,用GMCSF和IL4诱导7 d,获得未成熟白血病DCs,然后加入全病毒灭活流感疫苗（whole inactivated influenza vaccine, WIV）、裂解病毒流感疫苗（split influenza vaccine,SIV）或TNFα继续培养24 h。R显带法分析DCs染色体核型,流式细胞仪检测DCs表型,ELISA法测定DCs培养上清IL12的水平,CCK8法检测DCs诱导的CTL对自体白血病细胞的细胞毒作用。结果：19例AML患者中的15例及8例CML患者的MNC全部成功诱导出DCs。与TNFα刺激的白血病DCs相比,流感疫苗刺激的白血病DCs表面分子（CD80、CD83、CD86、HLADR）表达明显上调（P<005）,培养上清中IL12的分泌水平明显增加（P<0.05）,其诱导的CTL可显著杀伤自体白血病细胞（P<0.05）;WIV刺激的DCs在表型、IL12分泌水平及细胞毒作用方面均较SIV刺激的DCs显著增高（P<0.05）。结论： 流感疫苗促进髓系白血病源DCs表型成熟及IL12的分泌,增强其诱导的CTL对自体白血病细胞的杀伤作用。     

α干扰素促进急性髓性白血病来源的树突状细胞的成熟分化

目的:研究α干扰素(interferon alpha,IFN-α)对不同亚型的急性髓性白血病(acute myeloid leukemia,AML)细胞来源的树突状细胞(dendritic cell,DC)免疫表型及功能的影响。方法:5例初发AML患者(其中M2 1例、M4 2例、M5 2例)的外周血单个核细胞,在含GM-CSF、IL-4、TNF-α的无血清培养液中培养11 d,其后加入IFN-α-2a(1 000 U/ml)继续培养3 d,获得DC(IFN-AML-DC),光镜下观察细胞形态,流式细胞仪测定细胞免疫表型,同种异体混和淋巴细胞反应检测DC的免疫功能。结果:所有患者白血病细胞培养14 d后均转化为IFN-AML-DC,与正常人外周血单核细胞由GM-CSF IL-4 TNF-α培养获得的DC(monocyte-derived DC,MoDC)及AML细胞由GM-CSF IL-4 TNF-α培养获得的DC(AML-DC)相比,IFN-AML-DC具有典型成熟形态的细胞明显增多,表达CD83 细胞的比例显著增加,HLA-DR、CD86分子的表达水平增高,对同种异体T淋巴细胞的激活能力明显高于MoDC及AML-DC。结论:IFN-α能够促进AML-DC的体外成熟,形成更强的免疫激活能力,为AML的免疫治疗提供更有力的手段。     

Altered maturation of peripheral blood dendritic cells in patients with breast cancer

Tumours have at least two mechanisms that can alter dendritic cell (DC) maturation and function. The first affects the ability of haematopoietic progenitors to differentiate into functional DCs; the second affects their differentiation from CD14+ monocytes, promoting an early but dysfunctional maturation. The aim of this study was to evaluate the in vivo relevance of these pathways in breast cancer patients. For this purpose, 53 patients with invasive breast cancer were compared to 68 healthy controls. To avoid isolation or culture procedures for enrichment of DCs, analyses were directly performed by flow cytometry on whole-blood samples. The expression of surface antigens and intracellular accumulation of regulatory cytokines upon LPS stimulation were evaluated. The number of DCs, and in particular of the myeloid subpopulation, was markedly reduced in cancer patients (P<0.001). Patient DCs were characterized by a more mature phenotype compared with controls (P=0.016), and had impaired production of IL-12 (P<0.001). These alterations were reverted by surgical resection of the tumour. To investigate the possible role of some tumour-related immunoactive soluble factors, we measured the plasmatic levels of vascular endothelial growth factor, IL-10 and spermine. A significant inverse correlation between spermine concentration and the percentage of DCs expressing IL-12 was found. Evidence was also obtained that in vitro exposure of monocyte-derived DCs to spermine promoted their activation and maturation, and impaired their function. Taken together, our results suggest that both the above-described mechanisms could concomitantly act in breast cancer to affect DC differentiation, and that spermine could be a mediator of dysfunctional maturation of DCs.     

IL-12与IL-18联合刺激对树突状细胞分泌的exosome活性的影响

目的:观察IL-12与IL-18联合刺激对树突状细胞(dendritic cell,DC)分泌的exosome(DC derived exosome,Dex)活性的影响,为探索高效的exosome肿瘤疫苗奠定基础。方法:取正常健康人外周血单个核细胞诱导培养DCs,分别以IL-12、IL-18或IL-12+IL-18联合刺激DC,并设空白对照组、T细胞对照组,分别提取各组的Dex,Western blotting检测Dex中HLA-DR、CD83的表达,流式细胞仪检测CD54、CD80及CD86的表达;MTT法检测Dex刺激T细胞增殖的作用,ELISA法测定T细胞IFN-γ的分泌量。结果:IL-12、IL-18、联合组、空白组的Dex中均有HLA-DR、CD83蛋白表达;联合组Dex的CD54(323.67±44.06 vs 246.17±31.91、236.33±33.87、167.67±28.73,P<0.05)、CD80(406.37±39.18 vs 331.67±36.15、335.67±41.38、260.00±35.58,P<0.05)及CD86(390.50±38.06 vs 314.33±36.64、319.00±33.10、246.83±30.55,P<0.05)表达均高于IL-12组、IL-18组及空白组;联合组刺激T细胞增殖高于IL-12组、IL-18组及空白组、T细胞组(1.98±0.31vs 1.55±0.23、1.57±0.21、1.10±0.18、0.53±0.09,P<0.05);联合组刺激T细胞分泌IFN-γ水平高于IL-12组、IL-18组及空白组、T细胞组(436.67±61.80 vs 295.04±40.25、358.18±55.77、225.00±36.44、139.50±17.63,P<0.05)。结论:IL-12与IL-18联合刺激能增加Dex表达CD54、CD80及CD86,促进Dex刺激的T细胞的增殖及其IFN-γ的分泌。