急性脑血管病患者血清sIL-2R的检测

目的 :检测急性脑血管病患者血清 s IL- 2 R含量的变化。方法 :采用双抗体夹心法检测s IL- 2 R。结果 :出血性脑血管病患者 s IL- 2 R水平较正常对照组明显升高 ( P<0 .0 1 ) ,缺血性脑血管病患者 s IL- 2 R水平较正常对照组亦明显升高 ( P<0 .0 1 )。出血组与缺血组相比差异无显著性。结论 :血清 s IL- 2 R水平可反映急性脑血管病患者机体免疫状态 ,并可作为病情及判断预后的重要指标。     

口腔鳞癌患者术后可溶性白介素2受体水平的动态检测

目的 :探讨口腔鳞癌患者术后血清可溶性白介素 2受体 (s IL- 2 R)水平的变化在术后病情监测及预后评估中的意义。方法 :用 EL ISA法动态检测 4 7例口腔鳞癌患者术前 1 d及术后 0 .2 5、0 .75、1、3、6个月血清 s IL- 2 R水平同时检测 30例正常人血清s IL- 2 R水平。结果 :4 7例口腔鳞癌患者术前血清 s IL- 2 R水平明显高于正常人水平 (P <0 .0 0 1 ) ;术后 1周患者血清 s IL- 2 R水平较术前升高 (P <0 .0 5 ) ;其中 4 2例术后 6个月无复发或转移患者的血清 s IL- 2 R水平在术后 1周至 6个月呈下降趋势 ,至术后 6个月接近正常人水平 (P >0 .0 5 ) ;而 5例分别在术后 3～ 6个月复发或转移患者的血清 s IL- 2 R水平在术后 1个月开始明显上升(P <0 .0 5 )。结论 :s IL- 2 R对口腔鳞癌患者术后的病情监测及预后评估有帮助     

慢性肺心病急性发作期血清sIL-2R、C_3及CIC水平的检测及其临床意义

目的 :探讨慢性肺心病急性发作期血清中可溶性白细胞介素— 2受体 ( s IL-2 R)及补体 3成分 ( C3)、循环免疫复合物 ( CIC)水平及其临床意义。方法 :采用双抗体夹心 ELISA法 ,单向免疫扩散法、PEC—紫外分光法对慢性肺心病患者血清 s IL-2 R、C3及 CIC进行检测 ,并以直线相关法分析与 Pa O2 、 Pa CO2 的相关性。结果 :肺心病急性发作期患者血清 s IL-2 R、 CIC水平显著高于缓解期及正常对照组 ( P<0 .0 1 ) ,与 Pa O2 呈负相关 ( P<0 .0 5) ,与 Pa CO2 呈正相关 ( P<0 .0 1 ) ,而 C3低于缓解期及对照组。结论 :慢性肺心病患者血清 s IL-2 R、 CIC增高 ,C3下降 ,提示患者体内存在免疫功能紊乱 ,因此 s IL-2 R、C3及 CIC可作为临床观察慢性肺心病患者细胞免疫功能及病情判定的指标     

冠心病患者血清sFas和sFasL及sIL-2R的水平

目的 :探讨血清 s Fas、s Fas L和 s IL- 2 R水平与冠心病 (CHD)之间的关系。方法 :应用酶联免疫吸附双抗体夹心法测定 30例 CHD患者 (CHD组 )和 2 6例非冠心病患者 (对照组 )的血清 s Fas、s Fas L和 s IL - 2 R水平。结果 :CHD组患者 s Fas水平 (ng/ L)为 15 83.4 1± 174 .4 6 ,明显高于对照组 (1374 .5 5± 142 .4 2 ) ,P<0 .0 1。与对照组比较 ,CHD组患者血清 s IL- 2 R水平 (ng/ L)显著增高 (94 4 .5 0± 395 .5 9vs6 5 2 .4 5± 16 3.36 ,P<0 .0 1) ,且 CHD患者血清 s Fas水平与 s IL- 2 R水平呈正相关 (r=0 .4 18,P<0 .0 5 )。两组血清 s Fas L水平差别无显著性 (P>0 .0 5 )。结论 :高水平的血清 s Fas、s IL- 2 R与 CHD有关。高水平的血清 s Fas有可能通过抑制激活的 T细胞凋亡而导致冠状动脉事件的发生发展。     

类风湿关节炎患者血清和关节液细胞因子水平的变化及临床意义

目的　探讨幼年类风湿关节炎 (JRA)及类风湿关节炎 (RA)患者 IL- 6 ,IL- 8,s IL- 2 R和 TNF- α等细胞因子 (CK)水平的变化 ,及其与风湿活动的传统指标血沉 (ESR)和 C-反应蛋白 (CRP)的相关性 .方法　采用夹心 EL ISA法 ,对 30例 JRA和 34例 RA患者的血清中 ,4例 JRA,7例 RA,6例骨性关节炎 (OA)和 9例半月板损伤 (MT)患者的关节液中 IL- 6 ,IL- 8,s IL- 2 R和 TNF- α的水平进行检测 .结果　 130例 JRA,34例 RA患者血清 IL- 6和 s IL- 2 R的水平与对照组相差非常显著 (P>0 .0 1) ;30例 JRA患者血清 IL- 8水平与…     

肺炎支原体感染血清中IL-2、sIL-2R水平的检测及意义

目的 :对 2 5例肺炎支原体感染血清中 IL - 2、s IL - 2 R水平的检测及其意义的研究。方法 :采用双抗体夹心法对2 5例肺炎支原体感染急性期 (经血清明胶凝集法检测抗体效价 >1∶ 80确诊 )血清中的 IL- 2、s IL- 2 R进行检测。结果 :与正常人比较 ,MP感染急性期血清中的 IL- 2的表达降低 (P<0 .0 5 ) ,s IL- 2 R的表达明显升高 (P<0 .0 1)。结论 :MP感染病人早期出现暂时的细胞免疫功能低下。其他免疫力低下的慢性疾病易合并 MP感染     

乳腺癌患者手术前后血清中IL-2及sIL-2R水平

0　引言　肿瘤患者的免疫功能和肿瘤的发生 ,发展及预后密切相关 ,白细胞介素 - 2 (IL- 2 )通过与细胞 IL- 2受体 (IL- 2 R)结合 ,调节免疫细胞的增殖功能 ,发挥抗肿瘤作用 ,IL - 2 R不仅表达于激活的免疫细胞 ,还可以部分释放于血清中 ,称为可溶性 IL- 2 R(s IL- 2 R) .s IL- 2 R是一种免疫抑制因子 ,正常人血清中 s IL- 2 R含量极少 ,当机体免疫功能低下时 ,s IL- 2 R水平会显著升高[1 ] .我们通过对 5 8例乳腺癌患者手术前后血清中IL - 2及 s IL - 2 R水平进行了检测 ,探讨其与乳腺癌病理分类及预后的关系 .1　对象和方法1.1…     

反复呼吸道感染患儿血清可溶性白细胞介素-2受体检测的临床意义

目的 :探讨血清可溶性白细胞介素 - 2受体 (s IL- 2 R)对反复呼吸道感染患儿免疫功能的影响。方法 :采用双抗体夹心 ELISA法对 30例反复呼吸道感染 (RRTI)患儿血清 s IL - 2 R水平进行测定。结果 :RRTI患儿在疾病急性期血清 s IL- 2 R水平明显升高 ,与正常对照组相比差异显著(P<0 .0 1 ) ,恢复期血清 s IL- 2 R水平也降至正常范围 ;追踪观察发现 ,1年后转为支气管哮喘及慢性肺炎的患儿血清 s IL- 2 R水平持续升高。结论 :血清 s IL- 2 R水平异常升高是导致 RRTI患儿细胞免疫功能紊乱的重要因素之一 ,血清 s IL- 2 R水平的动态观察 ,可作为监测 RRTI患儿免疫功能、病情及预后的一项有价值的临床参数     

尿毒症患者腺苷脱氨酶活力与sIL-2R的检测

目的 :研究腺苷脱氨酶 (ADA)及 s IL- 2 R对尿毒症患者细胞免疫功能的影响。方法 :采用次氯酸测氨法及双抗体夹心法 ,对 34例尿毒症患者及 30例正常人血清 ADA活性及 s IL- 2 R进行了检测。结果 :尿毒症患者血清 ADA活性明显低于正常对照组 (P<0 .0 1 ) ,而 s IL- 2 R明显高于正常对照组 (P<0 .0 1 )。结论 :尿毒症患者血清 ADA活性降低及 IL- 2 R受体升高可能是导致免疫功能低下的重要原因     

烧伤患者血清sIL-2R水平的观察及临床意义

为探讨烧伤患者伤后免疫功能紊乱的可能机制以及烧伤患者病情监测、免疫状况评估的观察指标 ,动态观察了 35例大面积烧伤患者伤后一周、二周、三周及四周血清可溶性白介素 - 2受体 (s IL - 2 R)水平 ,并据烧伤严重程度分中、重、特重度烧伤组进行比较观察。结果发现 :(1)烧伤患者 s IL- 2 R水平明显高于正常对照组 (P<0 .0 1) ,至伤后四周仍无恢复 ;(2 )特重度烧伤患者 s IL- 2 R水平明显高于重度烧伤患者 ,重度烧伤患者 s IL- 2 R水平明显高于中度烧伤患者 (P<0 .0 1)。结论 :升高的 s IL- 2 R是影响烧伤患者免疫功能的众多原因中的重要原因之一 ,伤后 s IL- 2 R水平的观察对烧伤患者病情的监测及免疫状况的评估有一定的临床意义。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.