大鼠创伤修复中血小板源生长因子免疫组织化学的变化

目的：探讨创伤模型大鼠皮肤创面愈合过程中，血小板源生长因子（PDGF）表达的动态变化。方法：采用免疫组织化学研究伤后3，6，9，12d PDGF表达的变化。结果：创伤愈合PDGF表达在伤后6d呈强阳性，图像象分析，伤后6d与3d比较PDGF表达差异有显著性意义（P＜0．01）。结论：PDGF的表达变化和创面修复有密切关系，PDGF在创面愈合过程中有着规律性的变化并发挥十分重要的调控作用。     

糖尿病大鼠背部创面愈合过程中细菌量变化的动态观察

目的 :探讨糖尿病慢性溃疡愈合过程中细菌因素的影响以及创面局部使用血小板生长因子 (PDGF)治疗对细菌量变化的影响。方法 :在糖尿病大鼠背部切割圆形创面 ,创面分别涂 PDGF或基质。于伤后不同时间点采样 ,测定创面细菌量及创面面积和伤腔容积。结果 :1创面总细菌量及革兰阴性 (G- )菌量均在伤后第5日达到峰值 ,而后逐渐下降 ,但 G- 菌量下降明显快于总菌量 ,伤后第 7日已降至伤后第 1日水平 ,第 14日已检测不到 ;2伤后早期创面愈合速度较慢 ,第 7日后明显加快 ;这一快速愈合期与 G- 菌菌量快速减少期恰好重叠 ;3PDGF治疗组创面修复速度明显快于基质对照组和单纯致伤组 ,但其创面菌量变化与其余 2组比较无显著差异。结论 :创面 G-菌菌量变化可能影响糖尿病大鼠创面的愈合速度 ;创面使用 PDGF可加快创面愈合速度 ,但对创面菌量影响不大     

创面修复中C-myc原癌基因表达变化的观察

目的 观测创面修复中 C-myc原癌基因的表达变化,探讨 C-myc原癌基因表达变化在创面修复中的作用. 方法 应用原位杂交方法对临床患者断层供皮区创面愈合中 C-myc原癌基因表达变化进行了动态观察,并进行了半定量分析. 结果 临床创面愈合中 C-myc原癌基因有明显的规律性的表达变化,伤后 4 d 时, C-myc原癌基因在创面内有明显的表达 ,表达强度+～.伤后 10 d 时, C-myc原癌基因在创面内表达较伤后 4d 时增强,除分布在创面浅层的成纤维细胞胞浆和创缘上皮基底细胞胞浆以外,尚可见分布在血管内皮细胞和皮肤附属上皮细胞中,创面的中层和深层也可见有表达,多较密集分布,表达强度～.伤后 16 d 时, C-myc原癌基因在创面内表达的强度较伤后 10 d 时明显减弱,表达强度接近伤后 4d 时的水平,表达强度+. 结论 C-myc原癌基因表达与临床创面修复的过程有明显相关 ,C-myc原癌基因表达变化参与了临床创面修复过程,合理调控其表达变化有助于临床创面修复.     

创面修复中C-myc原癌基因表达变化的观察

目的：观测创面修复中C—myc原癌基因的表达变化，探讨C—myc原癌基因表达变化在创面修复中的作用。方法：应用原位杂交方法对临床患者断层供皮区创面愈合中C-myc原癌基因表达变化进行了动态观察，并进行了半定量分析。结果：临床创面愈合中C-myc原癌基因有明显的规律性的表达变化，伤后4d时，C-myc原癌基因在创面内有明显的表达，表达强度 - 。伤后10d时，C—myc原癌基因在创面内表达较伤后4d时增强，除分布在创面浅层的成纤维细胞胞浆和创缘上皮基底细胞胞浆以外，尚可见分布在血管内皮细胞和皮肤附属上皮细胞中，创面的中层和深层也可见有表达，多较密集分布，表达强度 ～ 。伤后16d时，C-myc原癌基因在创面内表达的强度较伤后10d时明显减弱，表达强度接近伤后4d时的水平，表达强度 。结论：C-myc原癌基因表达与临床创面修复的过程有明显相关，C-myc原癌基因表达变化参与了临床创面修复过程，合理调控其表达变化有助于临床创面修复。     

大鼠皮肤创面自然愈合中胰岛素样生长因子Ⅰ和增殖细胞核抗原的表达

目的:研究胰岛素样生长因子Ⅰ(IGF-1)对大鼠皮肤创面愈合过程的影响。方法:以大鼠为动物模型,在伤后1,4,7,11,14,和17d取材,利用免疫组织化学和图像分析,研究皮肤创面自然愈合过程中创面IGF-1前体、内源性IGF-1和增殖细胞核抗原(PCNA)的动态变化,以及它们与创面愈合相关因素成纤维细胞之间的关系。结果:在皮肤创面自然愈合过程中,内源性IGF-1表达量的变化与成纤维细胞活动、PCNA表达的变化基本相符,与IGF-1前体的变化关系不密切。结论:创面愈合过程中,在局部发挥生理效应的IGF-1主要来自创面以外的来源。内源性IGF-1与成纤维细胞增殖、活跃程度密切相关。     

重组人血小板源性生长因子凝胶剂促进糖尿病大鼠创面愈合的实验研究

目的 :研究重组人血小板源性生长因子 (rh PDGF BB)凝胶剂对糖尿病大鼠皮肤切割伤创面修复的作用及其量效关系。方法 :13只糖尿病大鼠在背部各制备 4个全层皮肤缺损创面 ,面积 2 .5 4 cm2 ,将创面分成5组 ,即 rh PDGF BB凝胶剂高剂量 (14 .0μg/ cm2 创面 )、中剂量 (7.0μg/ cm2 创面 )、低剂量 (3.5μg/ cm2 创面 ) 3组 ,凝胶剂基质对照组以及空白对照组。创面局部应用制剂 14 d,观察伤后不同时间各组创面面积、伤腔容积变化及组织学改变。结果 :伤后 14 d rh PDGF BB高、中、低剂量治疗组创面面积分别缩小至 (0 .2 2±0 .30 ) cm2、(0 .0 5± 0 .0 6 ) cm2和 (0 .32± 0 .32 ) cm2 ,中剂量组明显小于凝胶剂基质对照组 (0 .2 3± 0 .2 2 ) cm2 (P<0 .0 5 )和空白对照组 (0 .2 2± 0 .2 5 ) cm2 (P<0 .0 5 )。伤后 7d rh PDGF BB高、中、低剂量治疗组伤腔容积减小至(0 .0 4± 0 .0 3) m l、(0 .0 2± 0 .0 2 ) ml和 (0 .0 6± 0 .0 3) ml,只有中剂量组明显小于凝胶剂基质对照组 (0 .0 6±0 .0 3) m l(P<0 .0 5 )和空白对照组 (0 .0 7± 0 .0 5 ) m l(P<0 .0 5 )。组织学检查显示 ,经 rh PDGF BB中剂量治疗的创面伤后 7d肉芽组织生长活跃 ,其毛细血管胚芽与成纤维细胞数量显著多于其他组 ,伤后 14 d rh     

转化生长因子-β、碱性成纤维生长因子和血小板衍生生长因子在骨折愈合中的表达

目的:观察转化生长因子-β(TGF-β)、碱性成纤维细胞生长因子(bFGF)和血小板衍生生长因子(PDGF)在骨折愈合中的表达和分布情况,进而探讨其作用机制。方法:选用SD大鼠制作胫骨骨折愈合模型,伤后不同时期处死取材,分别进行组织学和TGF-β、bFGF和PDGF免疫组化染色观察。结果:(1)伤后3d开始形成原始骨痂。1周时肉芽组织中的间质细胞开始分化为软骨细胞,软骨形成后再进行软骨内化骨。4周时形成连接骨折端的桥接骨痂。(2)伤后早期血肿中炎性细胞表达bFGF、PDGF。伤后1周骨膜增殖细胞、肉芽组织中的成纤维细胞、内皮细胞、骨端骨细胞以及原始骨痂成骨细胞表达TGF-β、bFGF和PDGF。伤后2周软骨细胞表达TGF-β、bFGF和PDGF。结论:TGF-β、bFGF和PDGF有着各自的表达和分布特点,并共同调解骨原细胞的增殖和成骨细胞、软骨细胞的分化,最终完成骨折愈合。     

大鼠皮肤创面自然愈合中胰岛素样生长因子I和增殖细胞核抗原的表达

目的：研究胰岛素样生长因子I(IGF-1)对大鼠皮肤创面愈合过程的影响。方法：以大鼠为动物模型，在伤后1，4，7，11，14，和17d取材，利用免疫组织化学和图像分析，研究皮肤创面自然愈合过程中创面IGF-1前体、内源性IGF-1和增殖细胞核抗原(PCNA)的动态变化，以及它们与创面愈合相关因素成纤维细胞之间的关系。结果：在皮肤创面自然愈合过程中，内源性IGF-1表达量的变化与成纤维细胞活动、PCNA表达的变化基本相符，与IGF-1前体的变化关系不密切。结论：刨面愈合过程中，在局部发挥生理效应的IGF-1主要来自刨面以外的来源。内源性IGF-1与成纤维细胞增殖、活跃程度密切相关。     

复方芩柏颗粒促进大鼠Ⅱ度放射性皮炎创面愈合的研究

目的 探讨复方芩柏颗粒剂治疗大鼠Ⅱ度放射性皮炎的疗效及其机制,为临床治疗放射性皮炎提供新方法.方法 建立大鼠Ⅱ度放射性皮炎模型,随机分成3组,分别给予复方芩柏颗粒(实验组)、地塞米松(传统组)及生理盐水(对照组)处理.通过免疫组化染色及图像分析,测定各组大鼠伤后2、6、10、18、24 d的创面肉芽组织、愈合率及创面组织中碱性成纤维细胞(bFGF)的表达.结果 创面肉芽组织及愈合率:实验组在6、10、18、24 d各时相点较对照组及传统组均高,差异均有统计学意义(P均<0.05).bFGF的表达:实验组伤后6、10、18 d时bFGF表达高于对照组及传统组,差异均有统计学意义(P均<0.05).结论 复方芩柏颗粒外涂对大鼠Ⅱ度放射性皮炎创面的愈合有促进作用,其机制可能与促进bFGF的表达有关.     

烫伤创面愈合后的瘢痕形成与神经支配

背景:既往研究表明神经因素在创面愈合中具有重要调控作用,但有关神经调节与创面愈合后瘢痕形成之间的关系鲜有报道。目的:观察烧伤创面愈合过程中神经支配与创面愈合质量之间的关系。方法:将30只大鼠右侧T9~L1阶段脊神经根切断,制作失神经支配皮肤模型;然后在大鼠背部右侧失神经支配皮肤区域制作直径4cm的深Ⅱ度烫伤创面,设为模型组,左侧对称正常皮肤制作同样的创面,设为对照组,伤后连续观察创面变化,于7,14,21 d采用免疫组织化学法观察Ⅰ和Ⅲ胶原分泌情况,并计算Ⅰ/Ⅲ型胶原比例的变化,探讨创面愈合速度及愈合质量。结果与结论:模型组Ⅰ,Ⅲ型胶原分泌和Ⅰ/Ⅲ型胶原比值于伤后各时间点均显著低于对照组(P<0.05)。模型组Ⅰ型胶原分泌与伤后7,14,21 d逐渐增加(P<0.05),Ⅲ型胶原于伤后21 d时分泌明显增高(P<0.05),Ⅰ/Ⅲ型胶原比值与伤后21 d时明显降低(P<0.05)。结果证实,创面早期神经支配可以促进创面愈合,创面重塑期减轻神经支配可能会改善创面重塑质量。     

Tissue localization of beta receptors for platelet-derived growth factor and platelet-derived growth factor B chain during wound repair in humans.

The expression and localization of PDGF beta receptors and PDGF-AB/BB in human healing wounds was evaluated by immunohistochemical techniques and in situ hybridization. Expression of PDGF beta receptor protein and PDGF-AB/BB were analyzed in wound margin biopsies using the PDGFR-B2 and PDGF 007 antibodies. PDGF beta receptor expression was minor in normal skin. An increased expression of PDGF beta receptor protein was prominent in vessels in the proliferating tissue zone in wounds as early as 1 d after surgery and was apparent < or = 4 wk after surgery. There was also a concordant increase in PDGF beta receptor mRNA detected by in situ hybridization. PDGF-AB/BB was present in healing wounds as well as in normal skin. In normal skin, expression of PDGF-AB/BB was confined to peripheral nerve fibers and to solitary cells of the epidermis and of the superficial dermis. In wounds, infiltrating mononuclear cells also stained for PDGF-AB/BB. To identify cell types expressing PDGF AB/BB and PDGF beta receptors, respectively, we performed double immunofluorescence stainings. PDGF beta receptors were expressed by vascular smooth muscle cells and cells in capillary walls; the receptor protein could not be detected in neurofilament containing structures, T lymphocytes, or CD68 expressing macrophages. PDGF-AB/BB colocalized with neurofilaments, it was present in Langerhans cells of the epidermis and in HLA-DR positive cells located in the epidermal/dermal junction area. Of the macrophages infiltrating the wound, 43 +/- 18% stained positively for PDGF AB/BB. Since PDGF-AB/BB and PDGF beta receptors are expressed in the healing wound, two essential prerequisites for a role of PDGF in wound healing are fulfilled.     

微粒型血小板干粉促进小鼠皮肤创面愈合的实验研究

目的将过期的血小板制成微粒型血小板制剂,研究其与创面愈合及修复的作用关系。方法将过期的单采血小板冰冻干燥制成微粒型血小板制剂,制备的干粉质量为约0.28g/ml;分别采用ELISA的方法检测常规(液态)血小板与微粒型血小板制剂(复溶成液态)中VEGF、TGF-β1、PDGF的含量;将微粒型血小板制剂应用于全层皮肤缺损的小鼠背部皮肤创面,观察该制剂是否具有促进伤口愈合的功能;把小鼠随机分为生理盐水组、血浆组和微粒型血小板制剂组(每组每时相点6只),每只小鼠右侧均为生理盐水对照组,两组给药50μl(干粉用生理盐水复溶成液态)1次/d,观察伤后各组各时相点创面愈合情况,并应用免疫组化的方法检测各组术后5、7、10、14d创面皮肤组织CD31的表达情况。结果常规液态血小板和微粒型血小板制剂(液态)组中VEGF的含量,无统计学差异(t=-1.616,P>0.05),而微粒型血小板制剂组中TGF-β1、PDGF的含量较相应的常规液态血小板为高(t=-2.269、2.338,P均<0.05)。微粒型血小板制剂组在小鼠创伤后d5、7、10创面面积较盐水对照组和血浆组有明显的减小(t分别=2.997、3.427、10.78,t=2.599、2.331、6.407,两组P均<0.05);d5、7、10创面收缩率与盐水对照组相比有明显的提高(t=2.542、3.161、5.884,P<0.05),d7、10与血浆组相比,t=2.177、4.351,P均<0.05;实验组在创伤后d5、7、10创面组织CD31的积分光密度与盐水对照组相比有明显升高(t=-2.339、-3.553、-4.941,P<0.05)。d7、10与血浆组比较,t=-2.373、-2.412,P均<0.05。结论微粒型血小板干粉制剂中TGF-β1、PDGF较常规血小板中增高;其对小鼠皮肤创面的修复和愈合有促进作用。     

大鼠角膜碱烧伤后热休克蛋白70的表达(英文)

背景:角膜碱烧伤后损伤修复受许多因素的影响,热休克蛋白可促进变性、损伤蛋白质的迅速恢复或清除。目的:观察大鼠角膜碱烧伤后热休克蛋白70的表达及其与角膜损伤修复的关系。方法:检查大鼠眼无炎症及其他病变后,奥布卡因滴眼液点眼2次,棉签吸除结膜囊液体,将统一规格直径5mm的滤纸片浸泡于1mol/L NaOH溶液中10s,然后置于大鼠角膜中央30s制作大鼠角膜碱烧伤模型。分别于碱烧伤后6h,1,3,7,14,21d取材。结果与结论:RT-PCR、免疫组织化学染色、Western blot结果均显示热休克蛋白70mRNA和蛋白在角膜碱烧伤后1d即开始升高,7d时达高峰,14d后开始下降。苏木精-伊红染色及电镜观察显示角膜损伤在烧伤后6h即较明显,烧伤后7d逐渐恢复。提示大鼠角膜碱烧伤后热休克蛋白70的表达与碱烧伤后角膜损伤修复过程一致,参与了大鼠角膜碱烧伤后细胞的自我保护及修复过程。     

Fibrin biomatrix‐conjugated platelet‐derived growth factor AB accelerates wound healing in severe thermal injury

Controlled delivery of growth factors from biodegradable biomatrices could accelerate and improve impaired wound healing. The study aim was to determine whether platelet‐derived growth factor AB (PDGF.AB) with a transglutaminase (TG) crosslinking substrate site released from a fibrin biomatrix improves wound healing in severe thermal injury. The binding and release kinetics of TG‐PDGF.AB were determined in vitro. Third‐degree contact burns (dorsum of Yorkshire pigs) underwent epifascial necrosectomy 24 h post‐burn. Wound sites were covered with autologous meshed (3:1) split‐thickness skin autografts and either secured with staples or attached with sprayed fibrin sealant (FS; n = 8/group). TG‐PDGF.AB binds to the fibrin biomatrix using the TG activity of factor XIIIa, and is subsequently released through enzymatic cleavage. Three doses of TG‐PDGF.AB in FS (100 ng, 1 µg and 11 µg/ml FS) were tested. TG‐PDGF.AB was bound to the fibrin biomatrix as evidenced by western blot analysis and subsequently released by enzymatic cleavage. A significantly accelerated and improved wound healing was achieved using sprayed FS containing TG‐PDGF.AB compared to staples alone. Low concentrations (100 ng–1 µg TG‐PDGF.AB/ml final FS clot) demonstrated to be sufficient to attain a nearly complete closure of mesh interstices 14 days after grafting. TG‐PDGF.AB incorporated in FS via a specific binding technology was shown to be effective in grafted third‐degree burn wounds. The adhesive properties of the fibrin matrix in conjunction with the prolonged growth factor stimulus enabled by this binding technology could be favourable in many pathological situations associated with wound‐healing disturbances. Copyright © 2013 John Wiley & Sons, Ltd.     

Quantification of MSCs involved in wound healing: use of SIS to transfer MSCs to wound site and quantification of MSCs involved in skin wound healing

Mesenchymal stem cells (MSCs) are known to be effective in wound healing, but not much has been reported on quantitative correlations between MSCs injected into the wound site and MSCs that actually participate in wound healing. This study traced MSCs participating in wound healing by using small intestinal submucosa (SIS) as a cell carrier, identified their moving path and calculated the number of MSCs involved in wound healing. First, MSCs were isolated from the nude mouse and 1 × 10⁶ cells were seeded onto the centre of the SIS. MSC‐seeded SIS complexes were injected onto full‐thickness skin wounds made on the dorsum of nude mice. Tracing of MSC‐seeded SIS complex transplanted to the wound site revealed that 27.6% of the MSCs were migrated to the wound site at the first attempt. Second, repeated injection of additional MSCs did not increase the number of MSCs participating in wound healing beyond a certain constant maximum amount. The number of MSCs present in the wound site remains constant in the range 2–3 × 10⁵ from day 1 to day 10. The expression of skin regeneration‐related growth factors was confirmed by real‐time polymerase chain reaction (PCR) and enzyme‐linked immunosorbent assay (ELISA). MSCs participating in wound healing were found not only to suppress inflammation of the wound but also to increase the skin regeneration‐related growth factors that enable the recovery of the skin. An optimal number of about 3 × 10⁵ MSCs injected into the site was found to adapt themselves to the skin wound‐healing process effectively. Copyright © 2012 John Wiley & Sons, Ltd.     

大鼠皮肤创伤修复过程中转化生长因子β1和β3的表达

背景：转化生长因子β在组织创伤修复中发挥核心和关键作用.目的：观察转化生长因子β1和转化生长因子β3在大鼠皮肤瘢痕性创伤愈合过程中表达量及表达部位的变化.方法：制备大鼠皮肤全层切伤模型,长度1.5-2.0 cm,深及筋膜层.于伤后0 h,12 h,1 d,2 d,3 d,4 d,5 d,6 d,7 d处死大鼠,取损伤部位皮肤,采用免疫组织化学染色检测各时间点转化生长因子β1和转化生长因子β3的表达,并进行定量分析.结果与结论：免疫组织化学染色显示,在创伤愈合的早期阶段（伤后1-5 d）,转化生长因子β1和转化生长因子β3免疫阳性颗粒主要出现在上皮细胞、上皮基底层细胞胞浆、巨噬细胞等免疫细胞胞浆及肉芽组织中;随着创伤修复时间的持续,免疫阳性颗粒主要出现在真皮层的成纤维细胞及细胞外基质中.其中转化生长因子β1的表达在创伤后1-5 d最强,而转化生长因子β3在创伤后六七天时开始明显表达.可见在大鼠皮肤瘢痕性创伤愈合过程中,转化生长因子β1的表达先于转化生长因子β3,提示转化生长因子β1与胶原形成及创伤修复关系密切,而转化生长因子β3在愈合后期表达量有升高趋势,其可能与创伤后期的组织改建密切相关.     

红花注射液保存人胎羊膜贴敷皮肤切口

背景：血管内皮生长因子参与创伤组织修复,可促进与创面愈合.目的：观察红花注射液保存人胎羊膜对大鼠皮肤切创愈合中感染率及血管内皮生长因子表达的影响.方法：建立大鼠皮肤切创模型,建模后随机分成4组,分别以纱布、羊膜、及红花注射液处理过的胎儿面羊膜贴敷在切口表面,于切创后2,3,5,7 d 取大鼠切口皮肤组织应用免疫组织化学染色,通过计算机图像分析系统检测大鼠切口皮肤组织中血管内皮生长因子的表达情况,同时观察切口感染率.结果与结论：切创后2,3 d,红花＋羊膜组的血管内皮生长因子的表达明显高于其他3组（P 〈0.05）.切创后5 d,红花＋羊膜组感染率（5%）较其他3组明显减少（P 〈0.05）.结果证实,经红花注射液保存后的人胎羊膜在大鼠皮肤切创愈合的早期阶段,可促进创缘周围血管内皮生长因子的表达,将其贴附创面能明显减少创口的感染率、促进创口的愈合.     

Regulation of the postburn wound inflammatory response by gammadelta T-cells

Healing of the burn injury site is a critical component of the patient's successful recovery from this form of trauma. Previous studies from our laboratory have demonstrated that gammadelta T-cells via the production of growth factors are important in burn wound healing. Nonetheless, the role of these cells in burn wound inflammation remains unknown. To study this, wild-type (WT) and gammadelta T-cell receptor-deficient (delta TCR) C57BL/6 male mice were subjected to burn injury or sham procedure. Wound cells were collected by implantation of polyvinyl alcohol sponges beneath the burn site in injured mice or beneath uninjured skin in sham mice. At 3 days after injury, infiltrating cells, wound fluid, and skin were collected for analysis. Burn injury markedly increased skin tumor necrosis factor-alpha (TNF-alpha) and monocyte chemoattractant protein 1 levels. In WT mice, the numbers of infiltrating cells were similar between nonburn wounds and burn wounds. In contrast, deltaTCRmice displayed a 6-fold reduction in the cellular infiltrate. Burn injury in WT mice caused a marked increase in burn wound TNF-alpha, monocyte chemoattractant protein 1, and interleukin 6 content as compared with nonburn wounds, whereas in delta TCRmice, the burn-induced increase of TNF-alpha and interleukin 6 was not observed. The wound cell infiltrate at 3 days postinjury was devoid of gammadelta T-cells in WT mice. It was predominately of myeloid origin expressing high levels of CD11b and F4/80. In conclusion, these findings suggest that resident gammadelta T-cells are important in the recruitment of inflammatory cells and regulation of the inflammatory response at the wound site after thermal injury.     

外用中药对失神经支配创面VEGF表达的影响

目的：观察外用中药对大鼠失神经支配创面血管内皮细胞生长因子（VEGF）表达的影响。方法120只SD大鼠被随机分成4组：A组（龙血膏组）、B组（生肌膏组）、C组（失神经凡士林纱条换药组）和D组（非失神经凡士林纱条换药组）。对A组、B组、C组大鼠制造背部失神经支配的深创面模型,之后各组创面分别以龙血生肌膏、生肌愈皮膏、凡士林外用治疗,D组大鼠背部制作深创面模型,以凡士林治疗。于4、7、11、15、21d各时间点测算各组的创面愈合率,并对创面组织进行VEGF的免疫组化染色观察。结果各时间点A、B组创面愈合率均高于C、D组（P〈0.05）,C组低于D组（P〈0.05）。A、B、D组VEGF阳性表达第11天达峰值,C组第15天达峰值。第4、7、11天时A、B组VEGF阳性表达明显高于C、D组（P〈0.05）,C组低于D组（P〈0.05）;第21天时A、B、D组低于C组（P〈0.05）。结论外用中药对失神经支配创面的愈合有促进作用,可能与外用中药能增强失神经支配创面早、中期VEGF表达有关。     

白芨对大鼠创面愈合几个要素的影响

目的:研究白芨对大鼠创面愈合几个要素的影响,探讨其促进伤口愈合的机制。方法:大鼠麻醉后,在背部脊柱两侧1.5cm处切除两个直径约1.6cm的圆形,以随机数字表法选择其中一个创面局部涂抹白芨胶溶液;或在背部脊柱两侧1.5cm处各切开一约8cm长的全皮层切口,埋入聚乙烯醇(Polyvinylalcohol,PVA)海绵,随机数字表法选择一侧注入白芨胶溶液。结果:伤后3(或5),7,10,14,21d白芨治疗组创面残留面积百分率显著低于对照组,创面平均愈合时间提前2.5d,创面组织蛋白含量和羟脯氨酸含量也显著高于对照。伤后3,5,7d白芨组伤口巨噬细胞数量也显著提高。结论:白芨提高创面愈合的速度和愈合的质量,增加伤口巨噬细胞数量可能是其促愈合的重要机制之一。