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1.
Y.G. Tian M. Yue Y. Gu W.W. Gu Y.J. Wang 《Brazilian journal of medical and biological research》2014,47(9):753-758
Tibetan (TB) and Bama (BM) miniature pigs are two popular pig breeds that are used as
experimental animals in China due to their small body size. Here, we analyzed
single-nucleotide polymorphisms (SNPs) in gene fragments that are closely related to
growth traits [growth hormone (GH), growth hormone receptor
(GHR), and insulin-like growth factor
(IGF)-1)] in these pig breeds and a large white
(LW) control pig breed. On the basis of the analysis of 100 BMs, 108 TBs, and 50 LWs,
the polymorphic distribution levels of GH, GHR, and
IGF-1 were significantly different among these three pig breeds.
According to correlation analyses between SNPs and five growth traits - body weight
(BW), body length (BL), withers height (WH), chest circumference (CC), and abdomen
circumference (AC) - three SNP loci in BMs and four SNP loci in TBs significantly
affected growth traits. Three SNP sites in BMs and four SNP sites in TBs
significantly affected growth traits. SNPs located in the GH gene
fragment significantly affected BL and CC at locus 12 and BL at locus 45 in BMs, and
also BW, WH, CC, and AC at locus 45 and WH and CC at locus 93 in TBs. One SNP at
locus 85 in the BM GHR gene fragment significantly affected all
growth traits. All indices were significantly reduced with a mixture of alleles at
locus 85. These results provide more information regarding the genetic background of
these minipig species and indicate useful selection markers for pig breeding
programs. 相似文献
2.
Qingfeng Yin Qianqian Zhai Di Wang Jie Hai Meng Cao Jianling Wang Tao Wang 《International journal of clinical and experimental pathology》2015,8(11):15216-15221
We conducted a case-control study to investigate the association between interleukin (IL)-10-592C/A, -819C/T and -1082A/G polymorphisms and susceptibility to diabetic nephropathy. A hospital-based case-control study was taken in our study. A total of 172 patients with proven type 2 diabetes mellitus and 344 controls were recruited from the First Affiliated Hospital of Xinxiang Medical University between March 2012 and October 2014. Genotyping of IL-10 -592C/A, -819C/T and -1082A/G polymorphisms was done by done by PCR-RFLP methods. By the χ2 test, the distributions of the GG, GA and AA genotypes in IL-10 -1082A/G were significantly different between patients with diabetic nephropathy and control subjects (χ2 = 8.09, P = 0.02). By conditional logistic regression analysis, we found that the AA genotype of IL-10 -1082A/G was associated with an elevated risk of diabetic nephropathy compared to the GG genotype in codominant model, and the adjusted OR (95% CI) was 2.38 (1.23-4.57). In dominant model, the GA+AA genotype was associated with a significantly increased risk of diabetic nephropathy compared to the GG genotype in dominant model (OR = 1.47, 95% CI = 1.05-2.16). In recessive model, the AA genotype could influence the susceptibility to diabetic nephropathy when compared with the GG+GA in recessive model (OR = 2.08, 95% CI = 1.12-3.85). In conclusion, we suggested that IL-10 -1082A/G gene polymorphism was correlated with development of diabetic nephropathy, but no association was observed between IL-10 -819T/C and -592A/C and risk of diabetic nephropathy. 相似文献
3.
X.M. Wang S.J. Gao X.F. Guo W.J. Sun Z.Q. Yan W.X. Wang Y.Q. Xu D. Lu 《Brazilian journal of medical and biological research》2014,47(4):273-278
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to
multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the
potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a
treatment for drug-resistant breast cancer and to investigate the effect of
CIAPIN1 on the drug resistance of breast cancer in
vivo. We used lentivirus-vector-based RNAi to knock down
CIAPIN1 in nude mice bearing MDR breast cancer tumors and found
that lentivirus-vector-mediated silencing of CIAPIN1 could
efficiently and significantly inhibit tumor growth when combined with chemotherapy
in vivo. Furthermore, Western blot analysis showed that both
CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was
upregulated, after RNAi. Therefore, we concluded that lentivirus-vector-mediated RNAi
targeting of CIAPIN1 is a potential approach to reverse MDR of
breast cancer. In addition, CIAPIN1 may participate in MDR of breast
cancer by regulating P-glycoprotein and P53 expression. 相似文献
4.
F.E. Rosa R.M. Santos S.R. Rogatto M.A.C. Domingues 《Brazilian journal of medical and biological research》2013,46(3):207-216
Human epidermal growth factor receptor 2 (HER2) has been evaluated in breast
cancer patients to identify those most likely to benefit from herceptin-targeted
therapy. HER2 amplification, detected in 20-30% of invasive breast tumors, is
associated with reduced survival and metastasis. The most frequently used
technique for evaluating HER2 protein status as a routine procedure is
immunohistochemistry (IHC). HER2 copy number alterations have
also been evaluated by fluorescence in situ hybridization
(FISH) in moderate immunoexpression (IHC 2+) cases. An alternative procedure to
evaluate gene amplification is chromogenic in situ
hybridization (CISH), which has some advantages over FISH, including the
correlation between HER2 status and morphological features.
Other methodologies have also been used, such as silver-enhanced in
situ hybridization (SISH) and quantitative real-time RT-PCR, to
determine the number of HER2 gene copies and expression,
respectively. Here we will present a short and comprehensive review of the
current advances concerning HER2 evaluation in human breast
cancer. 相似文献
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6.
Zdeněk Verner Petra Čermáková Ingrid Škodová Bianka Kováčová Julius Lukeš Anton Horváth 《Molecular and biochemical parasitology》2014
Trypanosomatids are unicellular parasites living in a wide range of host environments, which to large extent shaped their mitochondrial energy metabolism, resulting in quite large differences even among closely related flagellates. In a comparative manner, we analyzed the activities and composition of mitochondrial respiratory complexes in four species (Leishmania tarentolae, Crithidia fasciculata, Phytomonas serpens and Trypanosoma brucei), which represent the main model trypanosomatids. Moreover, we measured the activity of mitochondrial glycerol-3-phosphate dehydrogenase, the overall oxygen consumption and the mitochondrial membrane potential in each species. The comparative analysis suggests an inverse relationship between the activities of respiratory complexes I and II, as well as the overall activity of the canonical complexes and glycerol-3-phosphate dehydrogenase. Our comparative analysis shows that mitochondrial functions are highly variable in these versatile parasites 相似文献
7.
PPARG,AGTR1, CXCL16 and LGALS2 polymorphisms are correlated with the risk for coronary heart disease
Jianwei Tian Shunying Hu Feng Wang Xuedong Yang Yuqian Li Congchun Huang 《International journal of clinical and experimental pathology》2015,8(3):3138-3143
Purpose: Our study was designed to explore the interaction between genes of PPARG, AGTR1, CXCL16 and LGALS2 and further investigate the association between genes polymorphisms and coronary heart disease (CHD). Methods: 90 CHD patients and 80 healthy individuals were enrolled in our study. Gene chip technology was used for checking four single nucleotide polymorphisms (SNPs) (PPARG rs1152002, AGTR1 rs5186, CXCL16 rs3744700 and LGALS2 rs7291467). MDR software was used to analyze gene-gene interactions. Odds ratio (OR) with 95% confidence interval (CI) were employed to evaluate the association of genes and CHD risk. Results: Genotypes and alleles distribution in case and control groups showed significant difference (P<0.05). And there exists interaction among genes. The model of PPARG×CXCL16 showed effects on the occurrence of CHD (OR=2.92, 95% CI=1.44-5.94). Meanwhile, the PPARG×AGTR1×CXCL16×LGALS2 model was associated with CHD susceptibility (OR=3.97, 95% CI=2.01-7.84). Moreover, we found that PPARG×LGALS2×CXCL16, was the best interaction model and it could significantly increase the risk for CHD (OR=3.37, 95% CI=1.71-6.63). Conclusion: PPARG rs1152002, AGTR1 rs5186, CXCL16 rs3744700 and LGALS2 rs7291467 polymorphisms may be closely related to the development of CHD. Moreover, there exist gene-gene interactions among these susceptibility genes. 相似文献
8.
The conserved E3 ubiquitin ligase component named SEL-10 in Caenorhabditis elegans and Fbw7 in mammals targets substrates for ubiquitin-mediated degradation through a high-affinity binding site called a Cdc4 phosphodegron (CPD). As many known substrates of Fbw7 are oncoproteins, the identification of new substrates may offer insight into cancer biology as well as aspects of proteome regulation. Here, we evaluated whether the presence of an evolutionarily conserved CPD would be a feasible complement to proteomics-based approaches for identifying new potential substrates. For functional assessments, we focused on LIN-45, a component of the signal transduction pathway underlying vulval induction and the ortholog of human Braf, an effector of Ras in numerous cancers. Our analysis demonstrates that LIN-45 behaves as a bona fide substrate of SEL-10, with mutation of the CPD or loss of sel-10 resulting in increased activity and protein stability in vivo. Furthermore, during vulval induction, the downstream kinase MPK-1/ERK is also required for LIN-45 protein degradation in a negative feedback loop, resulting in degradation of LIN-45 where ERK is highly active. As the CPD consensus sequence is conserved in human Braf, we propose that Fbw7 may also regulate Braf stability in some cell contexts. We discuss the implications of our findings for vulval development in C. elegans, the potential applicability to human Braf, and the value of a CPD-based predictive approach for human Fbw7 substrates. 相似文献
9.
Myxococcus xanthus development requires CsgA, a member of the short-chain alcohol dehydrogenase (SCAD) family of proteins. We show that CsgA and SocA, a protein that can replace CsgA function in vivo, oxidize the 2′-OH glycerol moiety on cardiolipin and phosphatidylglycerol to produce diacylglycerol (DAG), dihydroxyacetone, and orthophosphate. A lipid extract enriched in DAGs from wild-type cells initiates development and lipid body production in a csgA mutant to bypass the mutational block. This novel phospholipase C-like reaction is widespread. SCADs that prevent neurodegenerative disorders, such as Drosophila Sniffer and human HSD10, oxidize cardiolipin with similar kinetic parameters. HSD10 exhibits a strong preference for cardiolipin with oxidized fatty acids. This activity is inhibited in the presence of the amyloid β peptide. Three HSD10 variants associated with neurodegenerative disorders are inactive with cardiolipin. We suggest that HSD10 protects humans from reactive oxygen species by removing damaged cardiolipin before it induces apoptosis. 相似文献
10.
Eun-Joo Kim Jay C. Kwon Kee Hyung Park Kyung-Won Park Jae-Hong Lee Seong Hye Choi Jee H. Jeong Byeong C. Kim Soo Jin Yoon Young Chul Yoon SangYun Kim Key-Chung Park Byung-Ok Choi Duk L. Na Chang-Seok Ki Seung Hyun Kim 《Neurobiology of aging》2014
The hexanucleotide repeat expansion (GGGGCC) in chromosome 9 open-reading frame 72 (C9orf72) and mutations in the microtubule-associated protein tau (MAPT) and progranulin (GRN) genes are known to be associated with the main causes of familial or sporadic amyotrophic lateral sclerosis and frontotemporal dementia (FTD) in Western populations. These genetic abnormalities have rarely been studied in Asian FTD populations. We investigated the frequencies of mutations in MAPT and GRN and the C9orf72 abnormal expansion in 75 Korean FTD patients. Two novel missense variants of unknown significance in the MAPT and GRN were detected in each gene. However, neither abnormal C9orf72 expansion nor pathogenic MAPT or GRN mutation was found. Our findings indicate that MAPT, GRN, and C9orf72 mutations are rare causes of FTD in Korean patients. 相似文献
11.
Bin Jiao Xiaoyan Liu Beisha Tang Lihua Hou Lin Zhou Fufeng Zhang Yafang Zhou Jifeng Guo Xinxiang Yan Lu Shen 《Neurobiology of aging》2014
Recently, 3 rare coding variants significantly associated with Alzheimer's disease (AD) risk have been identified in western populations using whole exome sequencing method, including p.R47H in TREM2, p.V232M in PLD3, and p.T835M in UNC5C. To examine whether these variants are genetic risk factors in patients with AD from mainland China, we sequenced exon 2 of TREM2, exon 9 of PLD3, and exon 15 of UNC5C in Chinese Han population including 360 patients with AD and 400 control individuals. As a result, none of these 3 variants were identified in all subjects, however, 1 novel variant (p.A130V) in TREM2 and 4 novel variants (p.Q860H, p.T837K, p.S843G, and p.V836V) in UNC5C were detected in unrelated patients with late-onset AD. These findings suggest the 3 rare coding variants might not play an important role in AD risk in mainland China. 相似文献
12.
Clostridium difficile is the most common cause of hospital-acquired
diarrhea in patients treated with antibiotics, chemotherapeutic agents, and other
drugs that alter the normal equilibrium of the intestinal flora. A better
understanding of the risk factors for C. difficile-associated
disease (CDAD) could be used to reduce the incidence of CDAD and the costs associated
with its treatment. The aim of this study was to identify the risk factors for CDAD
in a cohort of Chinese patients in a Beijing hospital. Medical charts of a total of
130 inpatients (62 males and 68 females) with hospital-acquired diarrhea (45 with
CDAD; 85 without CDAD) were retrospectively reviewed. C. difficile
toxins A and B were detected in fecal samples using enzyme-linked fluorescence
assays. The drugs used by patients with and without CDAD before the onset of diarrhea
were compared. Factors that differed significantly between the two groups by
univariate analysis were analyzed by multivariate analysis using a logistic
regression model. Multivariate analysis showed that cephalosporin treatment was
associated with a significantly higher risk of CDAD in hospitalized patients, while
treatment with glycopeptides was significantly associated with a reduction in CDAD
(P<0.001 for cephalosporin; P=0.013 for glycopeptides). Our data confirmed
previous findings that empirical treatment with cephalosporins is positively
associated with CDAD compared to individuals using other CDAD-related drugs.
Additionally, we showed that treatment with glycopeptides was negatively associated
with CDAD, compared to individuals using other CDAD-related drugs. 相似文献
13.
Adriana Vaz dos Santos Cristiane Pinheiro Pestana Karen Rafaella da Silva Diniz Mário Campos Cláudia Bueno Abdalla-Carvalho Ana Lúcia Zuma de Rosso João Santos Pereira Denise Hack Nicaretta William Luciano de Carvalho Jussara Mendonça dos Santos Cíntia Barros Santos-Rebouças Márcia Mattos Gonçalves Pimentel 《Neuroscience letters》2010
In the last decade, several genes have been linked to Parkinson's disease (PD), including GIGYF2, ATP13A2 and GBA. To explore whether mutations in these genes contribute to development of PD in the Brazilian population, we screened 110 patients with early-onset PD. No clearly pathogenic mutations were identified in ATP13A2 and GIGYF2. In contrast, we identified a significantly higher frequency of known pathogenic mutations in GBA gene among the PD cases (6/110 = 5.4%) when compared to the control group (0/155) (P = 0.0047). Our results strongly support an association between GBA gene mutations and an increased risk of PD. Mutations in GIGYF2 and ATP13A2 do not seem to represent a risk factor to the development of PD in the Brazilian population. Considering the scarcity of studies on GIGYF2, ATP13A2 and GBA mutation frequency in Latin American countries, we present significant data about the contribution of these genes to PD susceptibility. 相似文献
14.
Ali Abdul Lattif Pranab K. Mukherjee Jyotsna Chandra Kim Swindell Shawn R. Lockhart Daniel J. Diekema Michael A. Pfaller Mahmoud A. Ghannoum 《International journal of medical microbiology : IJMM》2010,300(4):265-270
Infections due to Candida parapsilosis have been associated with the ability of this fungus to form biofilms on indwelling medical devices. Recently, C. parapsilosis isolates were reclassified into 3 genetically non-identical classes: C. parapsilosis, C. orthopsilosis, and C. metapsilosis. Little information is available regarding the ability of these newly reclassified species to form biofilms on biomedical substrates. In this study, we characterized biofilm formation by 10 clinical isolates each of C. parapsilosis, C. orthopsilosis, and C. metapsilosis. Biofilms were allowed to form on silicone elastomer discs to early (6 h) or mature (48 h) phases and quantified by tetrazolium (XTT) and dry weight assays. Surface topography and three-dimensional architecture of the biofilms were visualized using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), respectively. Metabolic activity assay revealed strain-dependent biofilm forming ability of the 3 species tested, while biomass determination revealed that all 3 species formed equivalent biofilms (P>0.05 for all comparisons). SEM analyses of representative isolates of these species showed biofilms with clusters of yeast cells adherent to the catheter surface. Additionally, confocal microscopy analyses showed the presence of cells embedded in biofilms ranging in thickness between 62 and 85 μm. These results demonstrate that similar to C. parapsilosis, the 2 newly identified Candida species (C. orthopsilosis and C. metapsilosis) were able to form biofilms. 相似文献
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16.
Introduction:
The status of msp1, msp2 and glurp allele frequency and the diversity of Plasmodium falciparum in Northwestern Colombia before the implementation of an artemisinin-combined therapy have been explored only by a few authors and in a relatively small number of samples from this highly endemic region.Objective:
To evaluate the frequency of msp1, msp2, and glurp alleles and the diversity of P. falciparum in two Colombian regions before the use of an artemisinin-combined therapy.Methods:
This study was part of a major anti-malarial efficacy trial designed as a random, clinically-controlled study for which 224 subjects were recruited. Region 2 of msp1 and msp2 (central region) were amplified by a nested PCR; glurp (region R2) was amplified by a semi-nested PCR.Results:
For msp1, five genotypes were observed, representing the K1, MAD20, and RO33 allelic families. All samples corresponded to a MAD20 150 bp allele. For msp2 (IC family), two alleles were detected and for glurp, eight were observed. A total 33 haplotypes were detected.Conclusions:
Analysis of glurpcan be used to successfully genotype parasite populations in the new studies in Colombia aimed at exploring Plasmodium spp population dynamics. In addition, analysis of msp1 and msp2 can also be of value for comparisons with past studies, but not when the objective is to study parasites obtained from the same patient in a reduced period of time; for instance, during treatment efficacy studies. 相似文献17.
Lesley Smyth Michelle Howell I. Nicholas Crispe 《Clinical & developmental immunology》1992,2(4):309-318
MRL-Mp-lpr/lpr mice contain phenotypically abnormal populations of T cells, and
exhibit an SLE-like autoimmune disease in which autoantibodies are a prominent
feature. We analyzed the phenotype and T-cell receptor Vß expression pattern in CD4+ T
cells of this mutant mouse strain to detect abnormalities that could explain the
autoimmunity. The CD4+ T cells contain two distinct abnormal populations. One of
these expresses B220 and HSA, and in these and other respects closely resembles the
accumulating CD4–CD8– population. The other expresses a high level of CD44 (Pgp-1),
and a high level of the 16A epitope of CD45, and so resembles post-activation T cells.
Both of these cell types are exclusive to MRL-Mp-lpr/lpr. We also identified V ß5- and
V ß11-positive CD4+ T cells, in both MRL-Mp-lpr/lpr and MRL-Mp-+/+ mice. We
conclude that autoimmune T cells can be detected in these mice, but that they are not the
cause of the accumulation of abnormal CD4+ and CD4–CD8–cells. 相似文献
18.
19.
Tomasz Ferenc Jan Wojciech Wroński Janusz Kopczyński Andrzej Kulig Ma?gorzata Sidor Liliana Stalińska Adam Dziki Jacek Sygut 《Pathology, research and practice》2009
The aims of this study were to analyze the cadherin/catenin adhesion complex in cells from abdominal and extra-abdominal aggressive fibromatosis tumors, and to estimate the correlation between the expression of the tested proteins and the clinical data of the desmoid patients. 相似文献