Effect of Tiangui Recipe (天癸方) on Serum Leptin and Pituitary Gonadotro pin in Androgen-Sterilized Rats

Ｓｕｆｆｉｃｉｅｎｔｎｕｔｒｉｔｉｏｎ，ｃｅｒｔａｉｎｅｎｅｒｇｙｒｅｓｅｒｖｏｉｒａｎｄｌｉｐｉｄｓａｒｅｎｅｃｅｓｓａｒｙ（1）ｆｏｒｉｎｉｔｉａｔｉｎｇａｎｄｍａｉｎｔａｉｎｉｎｇｔｈｅｒｅｐｒｏｄｕｃｔｉｖｅｆｕｎｃｔｉｏｎｏｆａｎｉｍａｌｓａｎｄ     

The Effect of Acupuncture on Bone Mineral Density in Postmenopausal Women

According to the TCM theory that the kidney isin charge of the bone, the authors carried out a studyon the effect of acupuncture on bone mineral densityin 40 postmenopausal women with osteoporosistreated by the method of reinforcing the kidney to     

Effect of Compound Recipe Gengniankang (更年康) on Senile Sexual Hormone and Expression of Estrogen Receptor in Bone of Climacteric Female Rats

Estrogenis a vital hormone to women's wholelife.It is aninevitable process that thelevel of ser-umestrogen decreases when women enter the agedphase,and that the ovary function is exhausted.Accompanying this process,theincidence of osteo-porosis is increased and affects the quality of lifeseverely.Using hormone replacement treat ment toprevent and treat osteoporosis is very effective.Itis proved that osteoporosis is related to the lack ofestrogen,which plays a veryi mportant role duringthe proc…     

Effect of Tiangui Recipe on Serum Leptin and Pituitary Gonadotropin in AndrogenSterilized Rats

Effect of Tiangui Recipe on Serum Leptin and Pituitary Gonadotropin in AndrogenSterilized Rats@孙斐@俞瑾     

Effect of Epimedium-derived Phytoestrogen on Bone Turnover and Bone Microarchitecture in OVX-induced Osteoporotic Rats

To investigate the preventive effect of epimedium-derived phytoestrogen(PE) on osteo-porosis induced by ovariectomy(OVX) in rats,11-month-old female Wistar rats were randomly di-vided into Sham,OVX and PE groups.One week after OVX,daily oral administration of PE(0.4 g·kg-1·day-1) started in PE group,and rats in Sham and OVX groups were given vehicle accordingly.The administrations lasted for 12 weeks.The biological markers including serum osteocalcin(OC) and urinary deoxypyridinoline(DPD) for bone turnover were evaluated at the end of the 12th week.On the 13th week,all the rats were sacrificed.The right proximal tibiae were removed,subjected to micro CT for determination of trabecular bone structure and then bone histomorphometry was per-formed to assess bone remodeling.The OVX rats were in a high bone turnover status as evidenced by increased bone formation markers and bone resorption markers.Treatment with PE could suppress the high bone turnover rate in OVX rats.Micro CT data revealed that PE treatment could ameliorate the deterioration of the micro-architecture of proximal tibiae induced by OVX,as demonstrated by greater bone volume,increased trabecular thickness and less trabecular separation in PE group in comparison with OVX group.The static and dynamic parameters of bone histomorphometry indi-cated that there were significant increases in bone formation variables and significant decreases in bone resorption variables between PE and OVX groups.The findings suggest that PE has a beneficial effect on trabecular bone in OVX rat model and this effect is possibly associated with stimulation of bone formation as well as inhibition of bone resorption.     

Effect of Kangquan Recipe (康泉方) on BAMBI Expression in Hypothalamic-Pituitary-Prostate in Rats with Benign Prostatic Hyperplasia

Objective: To investigate the effect of Kangquan Recipe (康泉方, KQR) on bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) expression and its mechanism in rats with benign prostatic hyperplasia (BPH). Methods: Forty-eight male Sprague-Dawley rats were divided into 6 groups using a random number table, with 8 in each group: the normal group (normal saline 10 mL/kg), the model group (normal saline 10 mL/kg), the finasteride group (0.5 mg/kg), the low-dose KQR group (3.5 g/kg), the middle-dose KQR group (7 g/kg), and the high-dose KQR group (14 g/kg). The 40 rats were subcutaneously injected with testosterone propionate after castration for 30 days to establish the BPH rat model except for those in the normal group. At the same time, the corresponding drugs were administered by gavage for 30 consecutive days. The effects of different doses of KQR on the protate wet weight, prostate volume and prostate index (PI) were observed. The changes in histopathology were monitored with hematoxylin-eosin staining. BAMBI protein and mRNA expression contents were determined by Western blot and quantitative real-time polymerase chain reaction, respectively. Results: All doses of KQR could decrease prostatic epithelial tissue proliferation. Compared to the model group, the high and middle-dose KQR significantly reduced prostate wet weight, prostate volume and PI; increased BAMBI protein expression in the hypothalamus, pituitary and prostate tissue; all doses of KQR up-regulated BAMBI mRNA expression in serum, prostatic fluid and prostate tissue (P<0.05 or P<0.01). Conclusions: KQR could inhibit the proliferation of rat prostatic tissue, promote BAMBI protein expression in the hypothalamic-pituitary-prostate of rats with BPH; and increase BAMBI mRNA expression in the blood, prostatic fluid and prostate tissue of rats with BPH, showing a dose-effect relationship. KQR can be used as a potential drug for the treatment of BPH.     

Effect of the Recipe of Zhishi Xiaopi Pill and its Ingredients on Gastric Emptying Rate and Plasma Motilin Level in Rats

To investigate the influence of the recipe of Zhishi Xiaopi Pill （ZSXPP） and its ingredients on gastric emptying and plasma motilin （MOT） level in rats. After the rats were douched by different herbs, cisapride or normal saline in different groups for 4 weeks, The gastric emptying rate and plasma MOT levels were measured respectively. The gastric emptying rate and plasma motilin levels were both increased significantly by using the total recipe of ZSXPP and some parts of the recipe. ZSXPP can increase the gastric emptying rate and plasma motilin levels in rats. The herbs can remove food and qi stagnancy and relieve flatulence; Zhishi, Houpu and Maiya were the main effective ingredients in this recipe.     

Effects of a Herbal Formula on Biochemical Markers of Bone Turnover in Ovariectomized Rats

Objective: To investigate the effect and mechanism of a Chinese herbal fo rmula MG in ameliorating estrogen deficiency bone loss. Methods: 1 0-month old female rats were randomly divided into three groups: ovariectomized (OVX), OVX treated with MG decoction (OVX-M) and OVX treated with estrogen injection (OVX-E). The urinary pyridinoline creatinine ratio (Pyd/Cr), deoxypyridinoline creatinine ratio (Dpd/Cr), and plasma alkaline phosphatase (ALP) were determined at the baseline, 1, 4, 8 and 12 postoperative weeks. Results: Our data showed an average increase of 93% in Pyd/Cr ( P<0.001), 180% in Dp d/Cr (P<0.005) and 59% in plasma ALP (P<0.001) respectively in the OVX group after o variectomy. Compared with OVX rats, the increase of the OVX-M group were preven t ed 39% in Pyd/Cr, 102% in Dpd/Cr and 19% in ALP respectively (P<0.05). Co nclusion: The herbal formula MG possesses a therapeutic effect on estrog en dependent bone loss by inhibiting the bone turnover as estrogen replacement.     

Effect of Depot Medroxyprogesterone Acetate （DMPA） on Bone Mineral Density in Women of Reproductive Age

Objective To compare bone mineral density （BMD） between users of intramuscular depot medroxyprogesterone acetate（DMPA ） and nonhormonal control subjects. Methods The study included 68 women aged between 25 and 40 years using depot medroxyprogesterone acetate for 24 months and 59 women aged between 25 and 40 years using nonhormonal contraception as control subjects. BMD of the lumbar spine and femoral neck were obtained using dual energy X-ray absorptiometry. Results At 24 months of treatment, as compared with baseline, the mean BMD in lumbar spine and femoral neck was decreased by 5.5% and 5.9%, respectively. Lumbar spine and femoral neck BMD in women who used DMPA were significantly decreased compared with the subjects in nonuser （P〈0.001）. Conclusion These results show BMD declined during using DMPA in women aged 25 -40 years old.     

Effect of Acupuncture on Serum Magnesium Level in Treatment of Migraine

Migraine is a common,obstinate and oftenrecurrent condition.Generally,it is considered thatthe pathogenesis is in close association withmetabolic disturbance of 5-hydroxytryptamine.Toapproach the mechanism of acupuncture fortreatment of migraine,58 cases of migraine weretreated by acupuncture to observe its effect on serummagnesium level.The results are reported as follows.     

Effect of Huan-shuai Recipe (缓衰方) on Expression of Extracellular Matrix in 5/6 Nephrectomized Rats

Ｈｕａｎ ｓｈｕａｉＲｅｃｉｐｅ（ＨＳＲ，缓衰方），ａＣｈｉｎｅｓｅｈｅｒｂａｌｍｅｄｉｃｉｎａｌｐｒｅｐａｒａｔｉｏｎｅｓｔａｂｌｉｓｈｅｄｂｙｔｈｅａｕ ｔｈｏｒｓ，ｐｏｓｓｅｓｓｅｓｔｈｅｍａｉｎｆｕｎｃｔｉｏｎｏｆｂｅｎｅｆｉｔｉｎｇｖｉｔａｌｅｎｅｒｇｙ，ｎｏｕｒｉｓｈｉｎｇｂｌｏｏｄ，ｐｒｏｍｏｔｉｎｇｂｌｏｏｄｃｉｒｃｕｌａ ｔｉｏｎ，ｐｒｏｍｏｔｉｎｇｄｉｕｒｅｓｉｓｔｏｅｌｉｍｉｎａｔｅｅｖｉｌＷｅｔｎｅｓｓａｎｄｐｕｒｇｉｎｇｔｈｅＴｕｒｂｉｄｎｅｓｓ，ｈａｓｂｅｅｎｕｓｅｄｔ…     

Effect of Combination of Bushen Jianpi Recipe (补肾健脾方) and Erythropoietin on Serum Tumor Necrosis Factor a in Patients with Anemia

Objective: To study the effect of treatment of renal anemia by combination of Bushen Jianpi Recipe (补贤健脾方, BJR, a Chinese experienced recipe for supplementing Shen and supporting Pi) and low dosage erythropoietin (EPO), and the influence of treatment on change of serum tumor necrosis factor α (TNF-α) so as to explore the possible mechanism of integrative Chinese and Western medicine (ICWM) in treating renal anemia. Methods: Patients with renal anemia were randomly divided into two groups, the ICWM group and the control group, supporting treatment such as dialysis, supplementing of ferrous, foliac acid and vitamin B12, was given to both groups. Additionally, to the ICWM group, 50 IU/kg of EPO subcutaneous injection for twice every week, and oral intake of BJR, one dose per day taken in two parts, were given, and to the control group, EPO alone, 50IU/kg by subcutaneous injecting, 3 times perweek, was given. The therapeutic course for two groups was 3 months. Blood levels of hemoglobin (Hb), hematoc     

Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats

Objective: To explore the protective effect and mechanism of Kuntai(KT) Capsule on angiotensin Ⅱ(AngⅡ)-induced hypertension in ovariectomized(OVX) rats. Methods: Fifty-four rats were randomly divided into 6 groups according to a random number table, 9 in each group: control, OVX sham+AngⅡ, OVX, OVX+AngⅡ,OVX+AngⅡ+E2, and OVX+AngⅡ+KT. OVX rats model was constructed by retroperitoneal bilateral ovariectomy.After 4 weeks of pretreatment with KT Capsule [0.8 g/(kg·d) and 17-β-estradiol(E2, 1.2 mg/(kg...     

Effect of Tiaoxin Recipe(调心方)on Spatial Memory and Energy Metabolism of Oxidation Injured Alzheimer''''s Disease Rats

Objective: To observe the effect of Tiaoxin Recipe (TXR) on the spatial memory, brain mitochondrial energy metabolism of oxidation injured Alzheimer's disease (AD) rats, and to explore the mechanism of TXR in treating AD. Methods: Eighty-eight SD rats were randomly divided into five groups (normal group, operative group, "AD" model group,TXR group and Aricept group). An oxygen free radical generation system (dihydroxy fumaric acid-trichloroferric-adenosine diphosphate, DHF-FeCl3-ADP) was used to create oxidation injured rat models mimic to AD; spatial learning and memory impairment (Morris water maze method), the activity of Succinate-oxidase, NADH-oxidase, CytC-oxidase (Clark oxygen electrode method) and the expression of cytochrome oxidase (CO) II mRNA (in situ hybridization method) were observed. Results: Compared with the normal group, the spatial memory, activity of CytC-oxidase and COII mRNA expression of oxidation injured "AD" rats were obviously decreased; TXR, however, could improve these funct     

Effect of Dachengqi Granules(大承气汤颗粒剂)on Level of Endotoxin in Enterogenous Endotoxemia in Rats

Objective:To study the effect of Dachengqi Granule (DCQG) on endotoxin level in rats ofenterogenous endotoxemia. Methods: Enterogenous endotoxemia model of rats was made by peritoneal in-jection of E. coli. In the treated groups, the rats were treated with different doses of DCQG, cefaclor andDCQG plus cefaclor respectively, starting from 3 days before to 1 day after modeling. Endotoxin levels inplasma, urine and stool were determined and compared. Results: Except for the cefaclor group, the plasmaand urine endotoxin levels in the treated groups were lower significantly (P＜0.05), while the endotoxinlevel in stool was obviously higher than that in the untreated model group. Conclusion: DCQG has the intes-tinal barrier protective effect including anti-bacteria, anti-inflammation and clearing endotoxin.     

Effect of Compound 962 Capsule on Liver Lipid Peroxidation and Indexes of Thymus Gland and Spleen in Rats

Objective: To investigate the effect of compound 962 capsule (abbre. as 962) on liver lipid peroxidation and indexes of thymus and spleen in aged rats.Methods: Rats were divided into young control, aged model, Piracetam, 962 middle dose (0.9 g/kg) and high dose (1.8 g/kg) groups. All test drugs were administrated for 1 month by gastrogavage. The liver lipid peroxidation was determined by thiobarbituric (TBA) method. The thymus index and spleen index were determined by weighing method.Results: 962 (middle and high dose) attenuated liver lipid peroxidation, increased the thymus index of aged rats. There was no effect on spleen index in all drug-treated groups.Conclusion: 962 could decrease liver lipid peroxidation and increase thymus index. It suggested that 962 might be beneficial in retarding aging process. This study was funded by Scientific Committee of Beijing (No. 951890600) and State Ministry of Personnel(No.l998)     

Effect of Huoxuequyu Recipe on Erythrocyte Ultrastructure and Membrane ATPase Activity in Rats with Passive Smoking

ＥｆｆｅｃｔｏｆＨｕｏｘｕｅｑｕｙｕＲｅｃｉｐｅｏｎＥｒｙｔｈｒｏｃｙｔｅＵｌｔｒａｓｔｒｕｃｔｕｒｅａｎｄＭｅｍｂｒａｎｅＡＴＰａｓｅＡｃｔｉｖｉｔｙｉｎＲａｔｓｗｉｔｈＰａｓｓｉｖｅＳｍｏｋｉｎｇＣＨＥＮＺｈｕｏ（陈琢），ＪｉＡＯＸｉｎｆｕ（焦新...     

Serum IL-10 Level in Allergic Rhinitis Patients and Its Effect on Serum Total IgE

disease.Itsepisodeisregulatedbymanykindsofcytokines.IL10isoneofthem,whichisanim portantnaturalimmuneregulatorandmainlypro ducedbyTh2cells.Itcaninhibittheantigen pres entingeffectsofmacrophagecellsandtheprolifer ationofactiveTcells,andsuppresstheintensityofinflammation[1,2].ItalsoplaysanimportantroleintheproductionofimmunoglobulineE.ThisstudysurveyedthelevelsofserumIL10inallergicrhinitispatientstoinvestigateitsrolesinthepath ogenesisofallergicrhinitisanditscorrelationtotheserumtotalimmunoglu…