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乳腺癌是当前危害妇女生命健康的最常见恶性肿瘤之一。为了提高乳腺癌患者生存率,测定乳腺癌组织中ER和PR水平已被作为制定治疗计划和评估预后的主要依据,近年来正日益受到临床医师的重视和关注。迄今为止国内已有不少实验室开展了ER和PR的检测分析,而且测定的方法也较 相似文献
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人体乳腺癌雌激素和孕激素受体检测75例分析 总被引:2,自引:0,他引:2
雌激素受体(ER)和孕激素受体(PgR)的检测已广泛应用于临床。本文用ABC法(Avidin-BiotinComplex method)标记了75例乳腺癌石蜡切片,并结合临床资料对标记结果进行分析,旨在探讨ER、PgR免疫组化测定的临床意义。 相似文献
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100例乳腺癌雌激素和孕激素受体的测定和分析 总被引:4,自引:0,他引:4
对100例乳腺癌选用ER和PR抗血清,采用ABC法进行免疫组化染色,结果发现:ER和PR同时阳性者为40例(40%),ER和PR同时阴性者为26例(26%);所有病例随访3~4年,40例ER和PR同时阳性中有1例复发,占2.5%,26例ER和PR同时阴性中有12例复发,占46.2%,二者有显著差异性(P<0.01)。结果提示:乳腺癌ER和PR同时阳性病预后较阴性病例好,因此,同时测定乳腺癌ER和PR对临床制定乳腺癌的治疗方案和判定预后有重大价值和意义。 相似文献
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乳腺癌中PS2基因与雌激素及孕激素受体 总被引:23,自引:0,他引:23
乳腺癌中PS2基因与雌激素及孕激素受体阚秀薛卫成乳腺癌激素疗法,最早于1896年由Beatson报道。1967年Jensen发现雌激素受体(EstrogenReceptor,ER),其后又发现孕激素受体(ProgesteroneReceptor,PR... 相似文献
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C—erbB—2癌基因与雌激素和孕激素受体在乳腺癌中的对比研究 总被引:3,自引:0,他引:3
采用ABC免疫组化染色对200例乳腺癌进行C-erbB-2,ER和PR测定,结果发现:(1)ER阳性112例中C-erbB-2阳性18例(16.1%),ER阴性88例中C-,erbB-2阳性57例(64.8%);PR阳性102例中C-erbB-2阳性16例(15.7%),PR阴性98例中C-erbB-2阳性59例(60.2%);(2)C-erbB-2阳性率与肿瘤大小和淋巴结转移有关,随访4年以上。 相似文献
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Background Recently objective quantification of immunohistochemical estrogen receptor (ER) and progesterone receptor (PgR) staining in
breast cancer by image cytometry has been predominantly performed by measuring the area of positively stained cells. However,
in sample preparations of immunostained hormone receptors, both the stained area and the intensity of staining vary. In this
study, we performed quantification of the stained area by measuring, tailing intensity using image cytometry.
Methods Quantitative analysis of ER and PgR immunohistochemistry was performed using image cytometry. The obtained values were presented
as % of positive staining (%PS). Comparison of %PS with values obtained by EIA and with clinicopathological features was performed.
Results The %PS values and the natural logarithm of the EIA levels of the hormone receptors showed a significant positive correlation
for both ER and PgR. The concordance of the results obtained by the two methods was 96.3% for ER and 73.7% for PgR. The ER-%PS
values of postmenopausal patients were significantly higher than those of premenopausal patients, whereas the PgR-%PS values
of the former group were significantly lower than those of the latter group.
Conclusions The quantification of ER and PgR in immunostained preparations using %PS as a parameter was reproducible and showed a high
correlation with values obtained by EIA. It was shown that only menopausal status affects hormone receptor levels when analyzing
the relationship between %PS measurements and clinicopathological features. 相似文献
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Pagona Lagiou Evangelia Samoli Areti Lagiou Christina Georgila Pantelina Zourna Anastasia Barbouni George Gkiokas Dorothy Vassilarou Annivas Tsikkinis Constantinos Sfikas Constantine E. Sekeris Chung-Cheng Hsieh Hans-Olov Adami Dimitrios Trichopoulos 《Cancer causes & control : CCC》2009,20(5):601-607
Objective It has been recently reported that expression of estrogen alpha (ER-α) and progesterone (PR) receptors in the normal mammary
gland is inversely associated with breast cancer risk among postmenopausal women. We investigated whether dietary intakes
are associated with the expression of ER-α and PR receptors in the apparently normal, as opposed to pathological, mammary tissue.
Methods In a study in Greece, we examined associations of dietary intakes with ER-α and PR expression in the adjacent-to-pathological
apparently normal mammary tissue of 562 women with either breast cancer (267 women) or BBD (299 women). Diet was assessed
through an extensive food frequency questionnaire and results were analyzed using multiple logistic regression.
Results Monounsaturated (p = 0.03) and, to a lesser extent, polyunsaturated lipids (p = 0.08) were positively associated with ER-α expression. Cereals and starchy roots were inversely associated with ER-α (p = 0.01), whereas milk and dairy products were inversely associated with PR expression (p = 0.02). Ethanol intake was non-significantly inversely associated with ER-α expression (p = 0.07).
Conclusions Our findings suggest that the weak associations of diet with breast cancer risk could be explained, to some extent, by effects
of diet on receptor expression in the normal mammary gland. 相似文献
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Over half of breast cancer cases are estrogen-dependent and strategies to combat estrogen-dependent breast cancer have been to either block the activation of estrogen receptor (ER) or diminish the supply of estrogens. Our previous work documented that estrogen-independent expression of progesterone receptor (PR) in MCF-7 cells markedly disrupted the effects of estrogen. In this study, we have developed an adenovirus-mediated gene delivery system to study the specific involvement of PR isoform A (PR-A) and PR-B in the anti-estrogenic effect and its mechanism of action. The results revealed that PR-B, but not PR-A, exhibited distinct anti-estrogenic effect on E2-induced cell growth, gene expression, and ER-ERE interaction in a ligand-independent manner. The anti-estrogenic effect of PR-B was also associated with heightened metabolism and increased cellular uptake of estradiol-17 beta (E2). We have also found that the B-upstream segment of PR-B alone was able to inhibit E2-induced ER-ERE interaction and cellular uptake of E2. Although PR-A alone did not affect E2-induced ER activity, it antagonized the anti-estrogenic effect of PR-B in a concentration-dependent manner. The findings suggest an important mechanism of maintaining a favorable level of ER activity by PR-A and PR-B in estrogen target cells for optimal growth and differentiation. The potential anti-estrogenic mechanism of PR-B may be exploited for breast cancer therapy. 相似文献
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Saji S Omoto Y Shimizu C Horiguchi S Watanabe T Funata N Hayash S Gustafsson JA Toi M 《Breast cancer (Tokyo, Japan)》2002,9(4):303-307
Since 1996 when estrogen receptor beta(ER beta) was discovered, much effort has been devoted to the question of the value of ER beta as a prognostic and/or predictive factor in breast cancer and its potential as a novel target for pharmacological intervention. When estrogen receptors are applied on sucrose gradients and quantified by ligand binding, we found that in contrast to ER alpha, which has a narrow tissue distribution, ER beta is expressed in many tissues including both normal and malignant breast tissue. Receptor protein levels in tissues can also be measured from the intensities of bands after Western blotting and can be quantified when purified and quantified receptor is used as a standard. With this technique, we found that there were some tumors which had over 600 fmol/mg of ER beta protein but no detectable estradiol binding. In such tumors, RT-PCR analysis revealed that ER beta cx is the only ER beta isoform present. ER beta cx is a splice variant which utilizes an alternative exon 8. This change in the C-terminus results in very poor binding to estradiol (E2) and has a dominant negative effect on ER alpha function. Immunohistochemical analysis with an ER beta cx specific antibody in 115 ER alpha-positive breast cancers revealed that about half of the samples expressed ER beta cx protein. Initial analysis of samples from patients with preoperative tamoxifen treatment revealed that ER alpha-positive tumors expressing ER beta cx and lacking PR seemed to be resistant to the anti-estrogen. We conclude that, in order to better characterize breast cancers and design appropriate therapy for individual patients, assays for ER beta cx must be made available to clinicians. 相似文献
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