冠内漂白对牙龈组织自由基含量的影响

目的：对狗牙冠内经３０％过氧化氢液漂白后，研究其牙龈组织中超氧化物歧化酶（ｓｕ－ｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，ＳＯＤ）、丙二醛（ｍａｌｏｎｄｉａｌｄｅｈｙｄｅ，ＭＤＡ）的含量变化。方法：用光化学扩增法测定ＳＯＤ；用硫代巴比妥酸比色法测定ＭＤＡ。结果：随漂白时间延长，ＳＯＤ含量逐渐下降，ＭＤＡ含量则逐渐增加，支持氧自由基的损伤机制。结论：冠内漂白引起牙龈组织中自由基含量增加，可损伤牙周组织。这种损伤可能是短暂的，也可能转变为慢性局部刺激因素，与其它因素协同作用，导致牙颈部外吸收     

热固化义齿软衬材料与基托树脂粘结的实验研究

测试了不同底胶、软衬材料中ＥＤＭＡ的含量及ＰＭＭＡ表面预处理对软衬材料与ＰＭＭＡ粘结强度的影响。结果表明，底胶Ｃ粘结效果最好，含有５％ＥＤＭＡ的软衬料与ＰＭＭＡ粘结强度最大，ＰＭＭＡ表面经处理后，粘结强度有明显提高。     

氧自由基在牙周炎患者龈组织中作用的研究

选择１８例成人牙周炎（ＡＰ）患者，取同一研究对象的牙周病变龈组织（实验组）和健康龈组织（对照组），制成组织匀浆，取上清液，测定超氧自由基（Ｏ－２）和两项目自由基损伤指标：超氧化物歧化酶（ＳＯＤ）、丙二醛（ＭＤＡ）。采用开放型双向序贯ｔ检验。结果表明：两组（Ｏ－２）、ＳＯＤ、ＭＤＡ水平明显差异，实验组（Ｏ－２）、ＭＤＡ水平高于对照组，ＳＯＤ水平低于对照组。提示：自由基参与牙周组织病变过程。     

手术配合冷冻、药灼骨腔防止角化囊肿刮治后复发21例报道

手术配合冷冻、药灼骨腔防止角化囊肿刮治后复发２１例报道ＯＰＥＲＡＴＩＯＮＣＵＲＥＫＥＲＡＴＯＣＹＳＴＣＯＭＰＡＴＥＤＷＩＴＨＲＥＦＲＩＧＥＲＡＴＩＯＮＡＮＤＢＵＲＮＵＩＯＡＤＲＵＧ许雪仙作者单位：浙江省中医院口腔科（３１０００６）颌骨牙源性角化囊肿单...     

颌面深部木质异物

颌面深部木质异物ＷＯＯＤＥＮＦＯＲＥＩＧＮＢＯＤＹＩＮＭＡＸＩＬＬＯＦＡＣＩＡＬＲＥＧＩＯＮ张瑛杨毅王善昌作者单位：上海第二医科大学附属新华医院口腔科（２０００９２）颌面部损伤无论在日常生活或战争时期都可发生，而损伤后一部分人在伤口中有异物存留。金属...     

应用SDH,LDH检测方法对Ca9—22,MEC—1,Tca8113细胞冷冻损伤…

对体外培养的三种肿瘤细胞进行冷冻处理，并对冷冻后细胞琥珀酸脱氢酶（ＳＤＨ）活力与乳酸脱氢酶（ＬＤＨ）释放量在不同冷冻温度和冻融周期条件下的变化，进行了测定。结果显示：冷冻对三种体外培养的癌细胞有明显的杀伤作用，其有效杀伤的临界温度为－４０℃～－５０℃。两次冻融的细胞杀伤率较高，继续增加冻融次数，细胞死亡率未见明显增加。ＬＤＨ释放测定结果表明，细胞膜的损伤是冷冻后立即发生的，在－４０℃冷冻复温后，９     

小剂量雷公藤多甙治疗RAU对血清SOD、MDA、微量元素改变的影响

选择４０例ＲＡＵ患者与５０例正常对照，应用小剂量雷公藤多甙、锌、铁制剂、丹参治疗，探讨治疗前后患者血清ＳＯＤ、ＭＤＡ及血清元素的改变。结果显示ＳＯＤ、ＭＤＡ的改变，实验组与对照组有显著性差异（Ｐ〈０．０１），治疗前、后对比又有显著性差异（Ｐ〈０．０１），而血清Ｚｕ、Ｃｕ／Ｚｎ、Ｆｅ、Ｍｎ治疗前后有明显差异（Ｐ〈０．０１），并与ＳＯＤ、ＭＤＡ的治疗前、后改变有明显相关性（Ｐ〈０．０１）。     

牙周炎患者龈组织中的自氧化作用与临床指数的相关性研究

目的：探讨内源性超氧基（Ｏ２）损伤在慢性成人牙周炎（ＡＰ）发生发展过程中的作用。方法：采用化学发光法、氧化法和比色法对４２例ＡＰ患者龈组织中的超氧基（Ｏ２）、超氧化物歧化酶（ＳＯＤ）活性和过氧化脂质产物（ＭＤＡ）含量进行测定。并将反映自氧化作用的ＳＯＤ．ＭＤＡ与临床指数进行直线相关及多元线形回归分析。结果：实验组与对照组 Ｏ２、ＳＯＤ、 ＭＤＡ活性水平有显著性差异（Ｐ＜ ０． ００１）; ＳＯＤ与周袋深度（ＰＤ）、附着丧失（ＡＬ）呈负相关（Ｐ＜０．００１）;ＭＤＡ与ＰＤ、ＡＬ呈正相关（Ｐ＜０．００１）。结论：氧自由基参与牙周病的病理过程。     

顺铂-白蛋白微球狗舌动脉灌注后的药物释放特性研究

本实验对ＣＤＤＰ－ＡＭＳ犬舌动脉栓塞后的药效学进行了研究，发现微球在体内具有缓释作用，起到药物存站的作用。结合血管密度的改变结果，较直接而具体地解决了产生ＣＤＤＰ－ＡＭＳ栓塞治疗临床效果的机理，认为其效果是通过局部较长时间持续的ＣＤＤＰ释放对肿瘤的杀伤作用和血流阻断后肿瘤组织缺氧、因，坏死的协同迭加作用，从而为临床更为广泛应用这一技术提供了实验数据。     

氧自由基在皮瓣组织静脉瘀血／再灌流损伤中作用的实验研究

本研究采用大鼠腹部岛状皮瓣静脉瘀血模型，观察不同静脉瘀血期组织中氧自由基的生成情况，用超氧化物歧化酶（Ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，ＳＯＤ）治疗。结果表明，皮瓣组织静脉瘀血８小时、１２小时丙二醛（Ｍａｌｏｎｙｌｄｉａｌｄｅｈｙｄｅ，ＭＤＡ）含量均高于（Ｐ＜０．０５）或显著高于（Ｐ＜０．０１）对照组。瘀血再灌流各组ＭＤＡ含量均显著高于（Ｐ＜０．０１）对照组。瘀血再灌流组与相同时间瘀血组相比，４小时、８小时组ＭＤＡ含量显著高于瘀血组。用ＳＯＤ（２０００ｕ／ｍｌ）在再灌流发生前动脉注射、能使皮瓣存活率从２８．６％（４／１４）提高到７８．６％（１１／１４）。提示：随着瘀血时间延长及再灌流的发生，氧自由基对皮瓣组织的损伤作用逐渐增强，ＳＯＤ在皮瓣组织静脉瘀血８小时后再灌流前动脉注射，能非常显著的提高皮瓣组织的存活率。     

Histopathologic studies of DMBA-induced mouse submandibular-gland tumors with or without cryoprobe treatment

Histopathologic observations of 9,10-dimethyl-1,2-benzanthracene (DMBA)-induced tumors in the mouse submandibular glands are reported using cryoprobe treatment. The experimental animals were divided into 2 groups; one received a carcinogen injection into the normal submandibular gland, and the other was injected with DMBA on the 14th day following cryosurgery of the gland using a -60 degrees C cryoprobe. Pathologic findings were classified as premalignant lesions or squamous-cell carcinomas with varying degrees of keratinization, fibrosarcoma and mixed carcinoma. There was also one case each of malignant pleomorphic adenoma and cystic adenoma. Tumor incidence was nearly the same in the 2 groups. Most of the carcinomas and sarcomas in the submandibular gland were induced during the 12th and 17th weeks. DMBA application during the proliferating stage which followed the cryosurgery did not enhance epithelial-tumor induction. During submandibular gland carcinogenesis, alterations in the granular convoluted tubule cells suggests they were the initial target cells undergoing malignant transformation. Squamous-cell carcinomas with varying degrees of keratinization were induced following squamous metaplasia in duct-like structures or multicystic lesions.     

Histopathologic studies of DMBA-induced mouse submandibular-gland tumors with or without cryoprobe treatment

Histopathologic observations of 9,10-dimethyl-1,2-benzanthracene (DMBA)-induced tumors in the mouse submandibular glands arc reported using cryoprobe treatment. The experimental animals were divided into 2 groups; one received a carcinogen injection into the normal submandibular gland, and the oilier was injected with DMBA on the Mill day following cryosurgery of the gland using a 60°C cryoprobe. Pathologic findings were classified as premalignant lesions or squamous-cell carcinomas with varying degrees of keratinization, fibrosarcoma and mixed carcinoma. There was also one case each of malignant pleomorphic adenoma and cystic adenoma. Tumor incidence was nearly the same in the 2 groups. Most of the carcinomas and sarcomas in the submandibular gland were induced during the 12th and 17th weeks. DMBA application during the proliferating stage which followed the cryosurgery did not enhance epithelial-tumor induction. During submandibular gland carcinogenesis, alterations in the granular convoluted tubule cells suggests they were the initial target cells undergoing malignant transformation. Squamous-cell carcinomas with varying degrees of keratinization were induced following squamous metaplasia in duct-like structures or multicystic lesions.     

冷冻治疗口腔恶性黑色素瘤的体会（附107例病例分析）

目的初步探索冷冻外科在处理口腔恶性黑色素瘤原发灶中的作用。方法回顾分析单纯手术治疗１３例、单纯冷冻治疗２４例和综合治疗７０例口腔颌面部恶性黑色素瘤的临床资料。结果三种方法治疗后３年和５年生存率分别是０．０％、０．０％;３７．５０％、３１．２５％及５７．１４％、３６．０７％。单纯手术组与冷冻组及综合治疗组存在统计学显著差异,但后两者疗效无统计学差异。结论提示综合治疗黑色素瘤是值得提倡的治疗方案。     

Nitrous oxide contamination during out-patient cryosurgery

Nitrous oxide concentrations have been measured spectrophotometrically in an out-patient clinic to assess pollution during simulated cryosurgery. Unacceptable levels, in excess of 2,000 ppm, were achieved during freezing and thawing and concentrations decreased only to 400 ppm when simple measures were taken to ventilate the clinic. The apparatus required a flow-rate of 11 l/min. It is concluded that scavenging apparatus should be used during cryosurgery with nitrous oxide if treatment is prolonged. Liquid nitrogen systems are preferable if excellent ventilation and scavenging apparatus are not available.     

口服硝酸盐对大鼠背部随意皮瓣的保护作用及机制探讨

目的:探讨口服硝酸盐对大鼠背部随意皮瓣的影响,以硝酸盐-亚硝酸盐-NO通路为切入点,探讨其可能的机制.方法:将24只雄性Wistar大鼠随机分为硝酸盐组、氯化钠组和对照组,每组8只.采用改良大鼠背部随意皮瓣制作方法造模.硝酸盐干预组在术前7 d及术后每天口服0.5 mmol/L硝酸钠,氯化钠组每天口服等量氯化钠,对照组...     

Lipid Peroxidation Levels and Total Oxidant/Antioxidant Status in Serum and Saliva From Patients With Chronic and Aggressive Periodontitis. Oxidative Stress Index: A New Biomarker for Periodontal Disease?

Background: In this study, levels of malondialdehyde (MDA), which is a significant product of lipid peroxidation (LPO), total oxidant status (TOS), total antioxidant capacity (TAOC), and the oxidative stress index (OSI), a novel value as a marker of periodontal disease activity, are investigated in serum and saliva from patients with chronic (CP) and generalized aggressive (GAgP) periodontitis. Methods: A total of 98 patients (33 with CP, 35 patients with GAgP, and 30 periodontally healthy controls) enrolled in the study. After clinical measurements and sample collection, the MDA level, TOS, and TAOC were measured by high‐performance liquid chromatography and a novel automatic colorimetric method. The OSI was calculated as [(TOS/TAOC) × 100]. Results: Although the salivary MDA levels and serum and salivary TOS and OSI values were significantly higher in the periodontitis groups than in the control group (P <0.05), the serum and salivary TAOC levels were significantly lower, and no significant difference in serum MDA levels was found (P >0.05). Furthermore, oxidative stress parameters were higher in the GAgP group than in the CP group (except the serum and salivary MDA levels and serum TAOC). Significant positive and negative correlations were observed between periodontal parameters and the MDA levels and TOS, TAOC, and OSI values (except serum MDA) (P <0.05). Conclusions: The present findings suggest that an increased TOS and decreased TAOC, rather than LPO, play important roles in the pathology of periodontitis and are closely associated with clinical periodontal status. Furthermore, the OSI may be a useful and practical parameter for evaluating periodontal disease activity.     

The impact of smoking status on antioxidant enzyme activity and malondialdehyde levels in chronic periodontitis

Background: The aim of this study is to investigate the impact of smoking status on the systemic and local superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px), and catalase (CAT) activities and malondialdehyde (MDA) levels in subjects with chronic periodontitis (CP). Methods: Sixty‐five CP patients (23 smokers [CP‐S], 23 former smokers [CP‐FS], and 19 non‐smokers [CP‐NS]) and 20 periodontally healthy non‐smoker controls (PH‐NS) were included in the study. After the clinical measurements, serum and gingival tissue samples were collected. SOD, GSH‐Px, and CAT activities and MDA levels in hemolysates and gingival tissue samples were spectrophotometrically assayed. Results: Blood MDA levels in all the periodontitis groups were higher than in the PH‐NS group but only the difference between CP‐FS and PH‐NS groups was significant (P <0.01). Gingival tissue MDA levels in the periodontitis groups were significantly higher than that in the control group (P <0.01). However, the control group had the highest gingival SOD, GSH‐Px, and CAT activities compared with all the periodontitis groups (P <0.01). The CP‐S group had the highest gingival MDA levels and SOD, GSH‐Px, and CAT activities among the periodontitis groups, whereas the lowest values were observed in the CP‐NS group (P <0.01). The blood and gingival MDA levels in the CP‐FS group were similar in the CP‐NS group, whereas they were lower than in the CP‐S group. Conclusions: Systemic and local MDA levels are increased by smoking in addition to the impact of periodontitis. The decreased local SOD, GSH‐Px, and CAT activities observed in periodontitis patients may increase with smoking.     

Effect of 2 episodes of cryosurgery on carcinogen-treated hamster cheek pouch

Hamster cheek pouches were treated with the topical carcinogen DMBA for 8 weeks. 2 episodes of cryosurgery, located within the area of previous carcinogen application enhanced the neoplastic process by comparison with matched controls. Comparison with previous studies suggests that the 2 episodes of cryosurgery may have a greater effect in provoking overt malignancy than a single episode of cryosurgery.     

Evaluation of oxidative status in patients with chronic periodontitis and polycystic ovary syndrome: A cross‐sectional study

1 Background

In patients with polycystic ovary syndrome (PCOS), chronic periodontitis (CP) contributed to increased oxidative stress (OS), owing to an increase in serum and salivary 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) and malondialdehyde (MDA) levels and a decrease in serum total antioxidant status (TAS) levels. The aim of the present study is to investigate salivary and serum 8‐OHdG and MDA levels as well as total antioxidant status (TAS) in females with CP and PCOS compared with healthy females.

2 Methods

Four groups, each consisting of 22 individuals, were: 1) women with both PCOS and CP (PCOSCP); 2) systemically healthy women with CP; 3) periodontally healthy women with PCOS (PCOSPH); and 4) periodontally and systemically healthy women (PH). Demographic and clinical periodontal parameters were measured. Oxidative parameters were evaluated in serum and salivary samples.

3 Results

Salivary 8‐OHdG levels in the PCOSCP and CP groups were statistically higher than those in both the PCOSPH and the PH groups (P < 0.05). There was no statistical difference between the PCOSCP, CP, and PCOSPH groups with regard to salivary MDA and TAS levels (P > 0.05). Highest serum 8‐OHdG and MDA levels and lowest serum TAS levels were seen in the PCOSCP group (P < 0.05). Serum 8‐OHdG and MDA levels in the PCOSPH group were higher than those in both systemically healthy groups (PH and CP) (P < 0.05). Salivary TAS levels were highest (P < 0.05) in the PH group. There was no statistical difference between the CP and PCOSPH groups, but serum TAS levels were lower than those in the PH group (P < 0.05).

4 Conclusions

CP, which led to an increase in serum and salivary 8‐OHdG and MDA levels and a decrease in serum TAS levels in patients with PCOS, contributed to increased OS. This effect was more prominent in serum levels than in salivary levels.     

Salivary Lipid Peroxidation in Patients With Generalized Chronic Periodontitis and Acute Coronary Syndrome

Background: Lipid peroxidation is a major consequence of oxidative stress and can be evaluated via malondialdehyde (MDA) levels. The present study aims to assess MDA levels in the saliva of patients with chronic periodontitis (CP) and acute coronary syndrome (ACS) and establish their correlation with periodontal clinical parameters, serum high‐sensitivity C‐reactive protein (hsCRP), and plasma fibrinogen. Methods: The study enrolled 64 patients stratified into four age‐ and sex‐matched groups: both ACS and CP, ACS only, CP only, and healthy controls. All patients were examined, periodontal clinical parameters were recorded, and saliva and blood samples were collected. Salivary MDA levels were measured using a spectrophotometric assay. A quantitative turbidimetric test was used for the measurement of serum hsCRP levels, and plasma fibrinogen levels were determined using an automated analyzer. Results: Salivary MDA levels were significantly higher in patients with both ACS and CP than in those with only ACS or only CP and healthy controls (P <0.05). There were significant positive correlations between salivary MDA levels and periodontal clinical parameters as well as biomarkers for cardiovascular events (P <0.001). Conclusions: To our knowledge, this study is the first to investigate salivary MDA levels in patients with ACS and their correlations with serum hsCRP and plasma fibrinogen levels. The results indicate that salivary MDA levels could be a biomarker for cardiovascular and/or periodontal disease.