解脲支原体和沙眼衣原体感染与不育不孕的关系探讨

目的　了解本市不育男性和不孕女性解脲支原体(Uu)、沙眼衣原体(Ct)的感染状况,探讨 Uu、Ct感染和不育不孕的关系。方法　对145例不育男性和172例不孕女性的生殖道分泌物进行Uu、Ct检测分析。结果　不育男性组Uu、Ct阳性率为35.9%、21.4%,明显高于男性对照组的14.0%和6.0%(P<0.01或0.05),不孕女性组 Uu、Ct阳性率为 39.5%、24.4%, 显著高于女性对照组的16.0%和6.0%(P均<0.01)。结论　Uu、Ct感染是导致不育不孕的重要因素,对不育不孕患者进行常规Uu、Ct检查,有利于对不育、不孕的诊断和治疗。     

解脲支原体感染与不孕不育症相关性分析

目的：调查本院生殖科不孕妇女生殖道解脲支原体（UU）感染及男性不育患者精液中解脲支原体感染的现状,分析解脲支原体感染与不孕、不育症相关性。方法：采用荧光定量聚合酶链反应（FQ-PCR）技术对240例不孕妇女、432例正常生育妇女的生殖道分泌物和73例男性不育、158例正常生育者的精液做解脲支原体检测。结果：女性不孕组阳性率为45．42％,对照组为阳性率为30．79％,男性不育组阳性率为49．32％,对照组阳性率为19．62％,不孕、不育组检出率明显高于对照组。结论：妇女生殖道、男性精液中感染解脲支原体是导致不孕不育的重要影响因素之一。     

288例不孕症妇女生殖道解脲支原体检测与分析

目的 :了解不孕妇女生殖道解脲支原体感染与各种不孕原因的相关性及其药物敏感情况。方法 :对不孕妇女 2 88例生殖道标本拭子进行解脲支原体培养及 10种抗生素敏感性试验。结果 :解脲支原体培养阳性 137例 (4 7.6 % ) ,原发不孕阳性率 36 .0 % ,继发不孕阳性率 5 8.4 % ,两组阳性率比较有显著性差异 (P <0 .0 0 5 ) ,而与不孕时间延长关系不明显 (0 .75 >P >0 .5 ) ,其对克拉霉素、交沙霉素、阿齐霉素敏感性高 ,敏感率分别为 98.5 %、97.8%、97.8%。环丙沙星、大观霉素、林可霉素敏感性差 ,敏感率分别为 10 .9%、35 .0 %、73.0 %。结论 :不孕妇女生殖道解脲支原体感染率高 ,说明UU感染与不育不孕症有非常明显关系 ,且对多种抗生物耐药 ,临床应开展解脲支原体培养与药敏试验 ,以指导用药。     

精液衣原体、支原体测定对男性不育的诊断价值

目的 :探索男性不育患者精液衣原体、支原体感染对精液生物学参数的影响。方法 :使用荧光定量PCR技术测定精液衣原体、支原体 ,采用超高倍显微镜检查精液质量。结果 :2 60例不育患者沙眼衣原体 (CT)阳性率为 14 .6% ,解脲支原体(UU)阳性率为 3 8.8% ,二者同时阳性占 7.3 % ,CT、UU总阳性率为 60 .8% ,而 2 0例健康对照组中仅UU阳性 1例 ,总阳性率为 5 % ,不育症中CT、UU阳性组较之阴性组精液液化时间明显延长 (P <0 .0 0 1) ,精子活动率降低 (P <0 .0 1) ,活动力得分减少(P <0 .0 0 1)。结论 :CT、UU感染是男性不育的主要病因之一。     

性病和不育不孕患者巨细胞病毒感染状况调查分析

目的　调查性病和不育不孕症患者巨细胞病毒感染状况 ,探讨性病—HCMV感染—不育不孕症的关系。　方法　采用酶联免疫吸附法 (ELISA)检测性病、不育不孕症患者和健康对照组血清中HCMV—IgM、IgG特异抗体 ,测定其活动性感染、既往性感染和总感染率。　结果　性病组与不育不孕组的HCMV总感染率分别为 94 73 3 %、90 2 2 % ,与对照组的 5 8 5 0 %相比较P <0 0 1。各组性病的HCMV总感染率分别为 97.14 %、94.67%、94.76%、94.64 %、92 .86% ,分别与对照组相比较差异具显著性P <0 .0 1。不育不孕组中有性病与无性病两组的HCMV总感染率分别为94 73 %和 83 .15 % ,两组之间相比较差异有显著性P <0 0 1,分别与对照组相比较P <0 0 1。　结论　性病—HCMV感染—不育不孕症三者之间存在密切联系 ,HCMV通过性传播途径感染可导致不育不孕症 ,必须加强性健康教育并对未生育和性病患者及性病感染高危人群进行CMV感染的检测 ,提高生育质量     

不孕妇女支原体感染及相关因素分析

目的 探讨女性不孕症与生殖道支原体感染的关系.方法 选择300例生育期不孕妇女分为原发性不孕症135例和继发性不孕症165例,观察支原体感染的检出情况、支原体感染与宫颈糜烂的关系和危险因素1ogistic逐步回归的分析结果.结果 原发性不孕组和继发性不孕组UU、MH及混合感染率均明显高于对照组,差异有统计学意义(χ2=4.12,P<0.01);宫颈糜烂患者支原体感染阳性率58.04%,明显高于宫颈光滑患者12.3%(χ2=3.99,P<0.01);1ogistic回归模型分析发现,继发性不孕的主要危险因素有人流、盆腔炎、输卵管病变、月经不调、子宫内膜异位症(简称E M7)、早年性交、支原体感染(宫颈分泌物U U+).结论 不孕妇女支原体感染率很高,且与宫颈糜烂密切相关.减少人流率,防治感染是降低继发性不孕的重要措施.     

不孕症与解脲支原体感染的临床分析

目的：探讨不孕症与解脲支原体感染的关系。方法：选取2008年1月-2011年1月我站诊治的210例不孕症患者,将其按原发不孕和继发不孕分为原发不孕组120例,继发不孕组90例,同时选取同期已生育女性100例为对照组。所选对象均采集宫颈、尿道分泌物进行解脲支原体检测。结果：210例不孕症患者经解脲支原体检测阳性143例,阳性率68.10%,正常对照组阳性18例,阳性率18.00%;不孕症患者经解脲支原体检测阳性率显著高于正常对照组阳性率,经统计学分析P〈0.05,差异有统计学意义。结论：解脲支原体感染是不孕症重要的病因,对解脲支原体感染控制非常重要,应对不孕症患者进行解脲支原体感染筛查,做到早控制,从而保护患者的生育力。     

支原体感染与不孕的相关性探讨

目的研究分析女性宫颈分泌物中解脲支原体的感染情况与不孕的关系。方法采用PCR法检测女性不孕患者宫颈分泌物中解脲支原体的感染情况。结果不孕组解脲支原体阳性率为36．83％,对照组解脲支原体阳性率为13．13％,两组间差异有统计学意义（P〈0．05）;原发不孕与继发不孕两组间差异无统计学意义（P〉0．05）。结论支原体感染与女性不孕症关系密切,需进一步研究支原体感染导致女性不孕的机制。     

解脲支原体感染与不育症关系的调查研究

434例男女不育不孕患者进行解脲支原体分高培养，其结果不育不孕组解脲支原体阳性者226例，阳性率521％，对照组阳性者17例，阳性率22．3％，两者差异有高度显著性（P＜0．01）。解脲支原体阳性226例经治疗后转为阴性者161例，其中40对转为阴性者有21对已怀孕，怀孕率53．0％，未转阴性者有18对仅3对怀孕，怀孕率17．0％，两者差异有显著性（P＜0.05）。提示解脲支原体感染与不育症有一定的关系。     

不育(孕)症精液及子宫颈粘液抗精子抗体检测

目的 :了解抗精子抗体 (AsAb)及其类型与不育 (孕 )症的关系。方法 :采用ELISA法检测AsAb ,支原体采用培养法 ,衣原体用金标法。结果 :12 0例不育症精液和 36例不孕症子宫颈粘液AsAb总阳性率为 2 6 .2 8% ;其中精液阳性率为 2 0 .83% ,子宫颈粘液阳性率为 44 .40 % ,男女间差异有显著性 (P <0 .0 1)。抗体类型中 ,IgM型男性阳性率 2 .5 0 % ,女性为 8.33 % (P>0 .0 5 ) ;IgG型男性阳性率 6 .6 7% ,女性为 36 .11% (P <0 .0 0 5 ) ;IgA型男性阳性率 12 .5 0 % ,女性为 30 .5 6 % (P <0 .0 5 )。精液中IgM和IgA间差异有显著性 (P <0 .0 1) ,但IgG与IgA和IgM间差异均无显著性 (P >0 .0 5 ) ;子宫颈粘液中IgM分别与IgA和IgG间差异均有显著性 (P <0 .0 5和P <0 .0 1) ,但IgG与IgA间差异无显著性 (P >0 .0 5 )。同时进行支原体、衣原体检测 ,感染与否和AsAb(+ )间差异均无显著性 (P >0 .0 5 )。结论 :精液和子宫颈粘液AsAb是不育 (孕 )症的重要原因 ,以IgG和IgA型为主 ;支原体和衣原体感染对产生AsAb无显著影响     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.