别嘌醇对急性心肌梗死患者再灌注心律失常的影响

目的:探讨别嘌醇预处理对急性心肌梗死患者经皮冠状动脉介入治疗术后再灌注心律失常的影响。方法:选取146例距离初次症状发作3~12 h内直接PCI的ST段抬高心肌梗死患者。随机分为实验组(n=74)和对照组(n=72),两组患者均予以冠心病介入标准化药物治疗,实验组在标准化药物治疗基础上给予别嘌醇顿服300 mg,术后100 mg/d维持。观察PCI术后24 h两组再灌注心律失常的发生情况及30 d主要心脏不良事件的发生率(死亡、心肌梗死或无计划的血运重建)。结果:实验组再灌注心律失常发生率18.9%,对照组再灌注心律失常发生率59.7%,两组比较差异具有统计学意义(P<0.05)。两组患者PCI术后30 d随访过程中均未发生主要心脏不良事件。结论:PCI术前别嘌醇预处理可对急性心肌梗死患者再灌注心律失常起到潜在保护作用,使患者远期预后获益。     

Determination of Ethyl Acetate and N-butanol Residual Volume in Epimedium Total Flavonoids

目的:建立气相色谱法测定淫羊藿总黄酮中有机溶剂乙酸乙酯和正丁醇残留量的方法.方法:采用毛细管气相色谱法,以SE-54毛细管柱为色谱柱,程序升温,氮气为载气,进样口温度为230℃,FID检测器温度为240℃.结果:乙酸乙酯和正丁醇残留量分别在1.606～16.06 mg/L(r =0.999 1)、1.622～ 16.22 mg/L(r=0.9993)的浓度范围内呈良好的线性关系,三批样品中乙酸乙酯和正丁醇残留量均符合中国药典有机溶剂残留量规定.结论:毛细管气相色谱法可作为淫羊藿总黄酮提取物中有机溶剂残留量的测定方法.     

高尿酸血症的临床治疗效果分析

目的:探讨临床治疗高尿酸血症的效果。方法:本次研究对象来源于我院2013年4月至2016年4月收治的高尿酸血症患者50例,依据治疗药物分组,其中对照组(n=25)采用别嘌醇,观察组(n=25)采用苯溴马隆,比较两组临床疗效。结果:对照组总有效率为76.0%,明显低于观察组92.0%(P0.05);两组治疗前血尿酸水平对比P0.05,治疗后观察组明显低于对照组(P0.05)。结论:苯溴马隆相较于别嘌醇治疗高尿酸血症患者降血尿酸效果更优,值得推广。     

高效液相色谱法测定人参中多菌灵农药残留含量

目的 建立了测定中药材人参中多菌灵农药残留量方法.方法 采用RP-HPLC法,Diamonsil C18色谱柱.流动相为甲醇-四氢呋喃-水(43∶2∶55),检测波长为277 nm.结果 平均回收率人参茎叶83.94%,RSD为2.42%(n=6);平均回收率人参根87.38%,RSD为2.68%(n=6).多菌灵在1.926～192.6 ng时,r=1.000 0,呈良好的线性关系.结论 本方法简便、快速、适合同类中药材中多菌灵农药残留量测定与安全监控.     

顶空毛细管气相法测定三七提取物中有机溶剂残留量

目的:建立三七提取物中7种有机溶剂残留量的测定方法。方法:采用顶空毛细管气相色谱法,以标准溶液加入法测定三七提取物中有机溶剂残留量。结果:在考察的浓度范围内7种有机溶剂呈现良好的线性关系(乙醇r=0.9994、正己烷r=0.9982、苯r=0.9999、甲苯r=0.9991、二甲苯r=0.9994、苯乙烯r=0.9997、二乙烯苯r=0.9932),回收率81.74%~111.2%,理论板数均大于15000,相邻峰的分离度均大于2,日内、日间精密度RSD均小于10%。结论:该方法简单、准确、灵敏度高、重现性好,排除了基质的干扰,适用于三七提取物中有机溶剂残留量的测定。     

赤芍提取物有机溶剂残留检测研究

目的: 建立赤芍提取物中2种有机溶剂残留量的检测方法.方法: 采用顶空气相色谱法,以正丙醇为内标物对乙醇、正丁醇进行检测.以FFAP毛细管柱(30 m×0.53 mm,1 μm)为色谱柱,柱温为60 ℃,载气为氮气,柱前压20 kPa,进样口温度为220 ℃,FID检测器,检测器温度为250 ℃,空气流速500 ml/min,氢气流速50 ml/min.结果: 乙醇、正丁醇分别在2.5～240 mg/L(r=0.999 6),0.5～48 mg/L(r=0.999 9)的浓度内呈良好的线性关系;平均回收率分别为99.95%、99.93%(RSD=3.5、3.2,n=9).结论: 顶空气相色谱法简便、快速、重现性好,适用于赤芍提取物中有机溶剂残留量的检测.     

磺胺二甲嘧啶有机溶剂残留的GC测定

目的:建立磺胺二甲嘧啶合成中所用有机溶剂丙酮残留量的测定方法.方法:采用毛细管气相色谱法,溶液直接进样,氢火焰离子化检测器(FID),以二甲基亚砜为溶剂,色谱柱为Supelcowax-10(30 m×0.32 mm,0.5μm),进样口温度160℃.检测器温度250℃,柱温120℃,载气为氮气.结果:在该色谱条件下丙酮与二甲基亚砜能达到完全分离,测得丙酮在2.08～6.25 μg/ml范围内线性关系良好(r=0.996 8),平均同收率分别为100.40%(RSD=1.10%).结论:该法简便、灵敏,易操作,可以作为磺胺二甲嘧啶原料药中有机溶剂残留量的检测.     

直接顶空气质联用法测定聚乳酸-乙醇酸微球中二氯甲烷的残留量

目的建立高分子材料聚乳酸-乙醇酸共聚物微球中二氯甲烷残留量的测定方法.方法采用毛细管气相色谱法,HP-5MS;柱温:50℃;进样口温度:280℃;载气为氮气;FID检测器;外标法计算含量.结果在该色谱条件下,测得二氯甲烷的线性范围为31.68～316.8g/mL(r=0.9998);平均回收率为97.86%,RSD为3.12%;检测限为0.002%;3批样品中二氯甲烷的残留量均符合要求.结论方法简单、快捷灵敏度高,可用于微球的制备过程中有机溶剂残留量的监控.     

芪灵降酸颗粒联合别嘌醇治疗痛风性肾病的临床研究

目的 观察芪灵降酸颗粒联合别嘌醇治疗痛风性肾病的疗效. 方法 将83例痛风性肾病患者随机分为治疗组(42例)和对照组(41例),治疗组给与芪灵降酸颗粒联合别嘌醇,对照组给与别嘌醇.结果 治疗组总有效率90.84%,对照组总有效率63.41%,经统计学分析,两组总有效率相比有显著性差异(P＜0.01). 结论 芪灵降酸颗粒联合别嘌醇治疗痛风性肾病疗效确切,值得推广.     

福州市汉族人HLA-B*5801基因多态性与别嘌醇所致严重皮肤不良反应关联性研究

目的探讨别嘌醇所致严重皮肤不良反应（SCAR）与HLA-B*5801基因的关系。方法将服用别嘌醇后致SCAR的21例患者作为别嘌醇SCAR组，30例服用别嘌醇后未出现SCAR的患者作为别嘌醇耐受组，另选取53例健康体检者作为正常对照组。对2组患者及正常对照组血液进行HLA-B*5801基因检测，观察其HLA-B*5801阳性基因例数及频率。结果别嘌醇SCAR组、别嘌醇耐受组及正常对照组的HLA-B*5801阳性基因数分别为19，4和10例。别嘌醇SCAR组HLA-B*5801阳性基因频率显著高于别嘌醇耐受组，2组比较差别有统计学意义[OR=61.75，95% CI（10.23，372.64），P<0.001];别嘌醇SCAR组HLA-B*5801阳性基因频率显著高于正常对照组，2组比较差别有统计学意义[OR=40.85，95% CI（8.16，204.64），P<0.001]。结论福州市汉族人服用别嘌醇所致SCAR与HLA-B*5801基因有关。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.