浙江绍兴卫氏并殖吸虫染色体核型的研究

本文报道绍兴兰亭卫氏并殖吸虫染色体核型为二倍体型(2n=22,n=11),成虫受精囊内存在精子,属于卫氏并殖吸虫的两性生殖型。结合绍兴兰亭肺吸虫病防治研究的有关资料分析,认为二倍体型卫氏并殖吸虫可使人体出现典型肺吸虫病症状.     

浙江绍兴卫氏并殖吸虫致病性的探讨

卫氏并殖吸虫分为两型 ,即受精囊内存在精子 ,染色体为二倍体 ( 2 n=2 2 )的基本型和受精囊内无精子 ,染色体为三倍体 ( 3n=33)的无精子型 [1 ]。Terasaki( 1 980 )将卫氏并殖吸虫的基本型称为两性生殖型 ,将无精子型称为孤雌生殖型 [2 ] 。卫氏并殖吸虫两型亲缘关系密切 ,但对人体致病性却有不同 ,一般认为三倍体型卫氏并殖吸虫为人兽共患的并殖吸虫 ,虫体在人体内可发育成熟并产卵 ,能引起并殖吸虫病的典型临床症状 ;二倍体型卫氏并殖吸虫为兽主人次的并殖吸虫 ,虫体在人体内一般不能发育成熟 ,也不能引起并殖吸虫病的典型临床症状 [3 ]…     

浙江乐清卫氏并殖吸虫染色体核型的研究

本文报道目前仍然有痰检虫卵阳性者存在的并殖吸虫病流行区——乐清马仰山卫氏并殖吸虫染色体核型为二倍体型(2n=22,n=11),成虫受精囊内存在精子,囊蚴较小,属于卫氏并殖吸虫的两性生殖型。结合乐清马仰山新近的并殖吸虫病流行病学资料分析,二倍体型能否引起人体并殖吸虫病的典型症状,值得探讨。     

卫氏并殖吸虫二倍体型与三倍体型染色体C显带的研究

本文以二倍体型及三倍体型卫氏并殖吸虫为材料,用BSG法对两者进行了染色体C显带的比较研究。结果表明,两者C带带型基本相同,并且具有基本一致的2、4～7号染色体的C带多态性。因而认为三倍体型卫氏并殖吸虫是二倍体型卫氏并殖吸虫的同源三倍体。本文系国内首次报告二倍体型卫氏并殖吸虫染色体的C带多态性及三倍体型卫氏并殖吸虫具有C带多态性的同源染色体的多种组合。     

浙江省永嘉县并殖吸虫DNA序列分析、形态及核型研究

目的　对浙江省永嘉县并殖吸虫的虫种及核型进行分析研究 ,探讨并殖吸虫的核型与临床类型的关系。　方法并殖吸虫虫种分析采用 DNA测序技术 ,结合形态观察。对感染猫 5 8d获取的幼虫的线粒体细胞色素 C氧化酶亚单位(CO1)部分基因及核糖体 DNA第二间区 (ITS2 )的基因序例进行测定。分离成虫的睾丸作染色体的核型分析。测量囊蚴、虫卵与来自不同疫区的囊蚴感染动物后不同虫龄虫体的大小 ,并观察其形态。　结果　CO1与 ITS2的基因序例与从Gen Bank检索到的卫氏并殖吸虫序例进行比较 ,结合对生活史不同阶段的虫体的形态观察 ,认定浙江省永嘉县的并殖吸虫为卫氏并殖吸虫 ;染色体数目分析 :75 %以上为 2 2条 ,即 2 n=2 2 ,并在绝大部分成虫的睾丸中观察到精子的存在 ;囊蚴大小平均为 331.8～ 338.3μm,虫卵平均长度和宽度分别为 76 .2 μm和 4 5 .6 μm,不同虫龄成虫大小 (5 .6～ 11.2 ) mm×(3.0～ 6 .2 ) mm,属二倍体型。　结论　永嘉县现分布的并殖吸虫为卫氏二倍体型。卫氏二倍体并殖吸虫能导致当地人肺部损害。     

卫氏并殖吸虫二倍体型与三倍体型染色体C带带型的比较研究

应用Sumner的BSG法,对卫氏并殖吸虫两个型的染色体C带带型进行了分析,结果表明两型间存在着差别。三倍体型卫氏并殖吸虫有两套染色体,其中一套在C带带型上与二倍体型相一致,另一套染色体则具有不同的带型,此差别突出表现在2、3、5、6和7号染色体上。表明两型间染色体结构不完全相同;卫氏并殖吸虫三倍体型可能是二倍体型的异源多倍体。     

卫氏并殖吸虫嵌合体的发现与卫氏并殖吸虫三种类型在哺乳动物体内的自然混合寄生

本文报告了卫氏并殖吸虫的一种新类型——嵌合体型的存在。嵌合体成虫生殖细胞的染色体数为22/33。虫卵大小介于卫氏并殖吸虫二倍体型与三倍体型虫卵之间。同时证实辽宁省宽甸县半拉江流域的哺乳动物体内有卫氏并殖吸虫三种类型的自然混合寄生。研究结果显示,两型的关系确实极为近缘。     

卫氏并殖吸虫生殖细胞第一次减数分裂各期的形态学观察

有学者认为,卫氏并殖吸虫分二倍体型和三倍体型,前者存在减数分裂。两型所致临床表现各异。日本学者 Miyazaki 推测湖北的卫氏并殖吸虫为三倍体型。我们1984年研究的鄂南卫氏并殖吸虫为二倍体型,本文又对鄂东卫氏并殖吸虫染色体核型及第一次减数分裂各期形态进行了观察。一、材料和方法按作者1984年的方法首先制备、观察染色体     

江西二倍体型卫氏并殖吸虫感染大鼠及其活力与侵袭力的研究

卫氏并殖吸虫染色体核型有两型，即二倍体型（２ｎ＝２２）和三倍体型（３ｎ＝３３）。两型并殖吸虫在宿主特异性、宿主相容性及致病性等方面均存在差异。不同品系的卫氏并殖吸虫在大鼠体内的发育，不尽相同。同一品系、不同地理分布的卫氏并殖吸虫，在大鼠体内的发育也有...     

辽宁省凤城县两型卫氏并殖吸虫的发现及其分布

<正> 为了解本县卫氏并殖吸虫的类型及其分布状况,选择4条河流,9个自然村对卫氏并殖吸虫类型观查了囊蚴,成虫、虫卵形态及成虫的染色体核型,以及蛄、犬及居民感染染情况,调结果如下。 一、蝲蛄感染吸虫囊蚴情况 4条河流,9个自然村共检查蝲蛄193只,阳性率为31.09％(60/193),阳性蝲蛄含囊蚴数从1(最低)至224(最含)。 二、成虫的大小及染色体观察 在伙荣沟村、三合村及秋岭村各解剖1只犬,在犬肺中均发现有感染。三合村的犬肺中仅发现2条未成熟的童虫。对伙荣沟及秋岭两地的成虫封片染色进行比较测量,并以空干法对单个虫体生殖腺进行培养后检查染色体,确切证实靉河上游金家河的伙荣沟村的卫氏并殖吸虫为三倍体型,靉河下游秋岭村的卫氏并殖吸虫为二倍体     

片吸虫核型的研究

本文用空气干燥法制作片吸虫染色体,测量结果二倍体型染色体数为2n=20,n=10.三倍体型染色体数为3n=30,n=10。两者核型相似。二倍体型虫体与巨片吸虫相似,三倍体型虫体与肝片吸虫相似。一部分三倍体型标本中发现少数细胞具20个染色体,由10个单价染色体和10个双价染色体组成。经测量,这些染色体可分为10对,每对由1个单价染色体和1个双价染色体组成。     

中国并(狸)殖吸虫病研究现状(1949—1994)

中国并（狸）殖吸虫病研究现状（１９４９—１９９４）陈桂光福建医学院福州３５０００４并（狸）殖吸虫病系一种人兽共患疾病，早在１８７９年ｍｇｅｒ于台湾首次由病人尸检中从肺脏发现这种吸虫（通称肺吸虫），同时Ｍａｎｓｏｎ在厦门从一名到过台湾的患者血痰内查获该...     

Immunoelectrophoretic study on common antigens of S?o Louren?o da Mata and Belo Horizonte strains of Schistosoma mansoni adult worms and Biomphalaria snails.

Immunoelectrophoretic studies on common antigens were carried out by using rabbits sera immunized against S?o Louren?o da Mata and Belo Horizonte strains of Schistosoma mansoni adult worms and antigens of Biomphalaria glabrata pigmented (Jaboat?o--PE); B. glabrata albino (Belo Horizonte--MG) and B. straminea (S?o Louren?o da Mata, PE). Furthermore, the reverse approach was proceeded, namely, sera anti Biomphalaria snails produced in rabbits were tested against both strains of Schistosoma adult worm antigens. The analysis of the common antigens between the SLM strains of S. mansoni adult worm and B. glabrata pigmented showed 8 to 9 precipitin bands, 3 bands with B. glabrata albino and only 1 band with B. straminea crude extracts. On the other hand, the BH strain of S. mansoni adult worm antisera produced 6 to 7 bands with B. glabrata pigmented, 5 bands with B. glabrata albino and 1 band with B. straminea antigenic extract. Biomphalaria snails crude extracts were fractionated by Sephadex G-100 column and three fractions were collected from each snail strain. The fractions were tested with anti SLM and BH strains of S. mansoni adult worm sera by immunoelectrophoresis. The common antigens fractionated from Biomphalaria snails crude extracts and those found for both strains of S. mansoni adult worm mostly existed in the first fraction and they were estimated to have molecular weight over 158,000 daltons. In our laboratory, it was found a relationship between the antigenic similarities and experimental infection rates of S. mansoni towards Biomphalaria snails so that more bands were seen with increasing infection rates of S. mansoni.     

斯氏狸殖吸虫成虫可溶性抗原蛋白质分析

目的分析和检测斯氏狸殖吸虫成虫可溶性抗原的特异抗原组分,为建立特异、敏感的免疫学诊断方法、研制抗斯氏狸殖吸虫药物和疫苗提供理论基础。方法用聚丙烯酰胺凝胶电泳(SDS-PAGE)、双向电泳(Two-dimension-al gel electrophoresis,2-DE)和免疫印迹(Western blot)等方法,对斯氏狸殖吸虫成虫可溶性抗原进行分析。结果SDS-PAGE分离出22条蛋白带,其中主带8条,分子质量单位为13~64 ku,未见65 ku以上条带。Western blot显示20条显色带,主带6条,分子质量单位分别为13、18、22、28、35和64 ku。2-DE电泳分离出斯氏狸殖吸虫成虫可溶性抗原48个多肽斑点,分子质量单位为14~70 ku,绝大部分分布于pI 3.10~7.14,少数分布于pI 8.78~9.85,在pI 7.14~8.78之间几乎未见多肽斑点。Western blot分析出其中的10个主要多肽斑点能被斯氏狸殖吸虫病患者血清识别,大部分为集中在酸性区域的小分子多肽。结论斯氏狸殖吸虫成虫可溶性抗原经SDS-PAGE电泳分离出的22条蛋白带中,有6条蛋白含量较高,能被斯氏狸殖吸虫病患者血清识别。2-DE电泳分离出的48个多肽斑点中,有10个含量较高,能被斯氏狸殖吸虫病患者血清识别,大部分为偏酸性的小分子多肽。     

关于扁囊并殖吸虫的研究

自1985年起作者发表了系列研究文章，详细观察了扁囊和卫氏并殖吸虫生活史中的囊蚴、后尾蚴、成虫、虫卵等阶段的形态，上述两种囊蚴的结构相似，后尾蚴的脱囊率、成虫在犬体内的寄生部位、成熟时间等均相似。后尾蚴、成虫的形态及成虫各部位的大小经统计学处理无明显差异。对这两种成虫相应的六个部位的体棘进行了扫描电镜观察未见明显差异。对扁囊、卫氏并殖吸虫的囊蚴和成虫的全虫蛋白分别进行了盘状电泳和等电点聚焦电泳分析，发现这两种虫盘状电泳和等电点聚焦电泳的蛋白质区带数目与模式均一致。并用等电点聚焦电泳的方法检测了这两种虫的苹果酸脱氢酶、酯酶、过氧化物酶等三种同工酶，结果显示这两种虫的三种同工酶的酶带数目及等电点分布完全一致。此外还对扇囊和卫氏并殖吸虫的基因组DNA限制性内切酶酶谱和PWB19片段探针的Southern印迹杂交进行了分析，结果基本相同。上述各项研究结果均显示扁囊和卫氏并殖吸虫之间无明显差异，鉴于此，我们认为扁囊并殖吸虫似应为卫氏并殖吸虫的同物异名。     

Transcortin and vitamin D-binding protein levels in mouse serum

The influence of age, sex and strain on the serum concentration of transcortin (corticosteroid-binding globulin) and vitamin D-binding protein (DBP) in mice was investigated. The effect of age was studied in two strains, C57BL/6JPfd and BALB/cmHeAPfd. The concentration of transcortin and DBP increased with age. In young animals the concentration of each protein showed a significant strain difference, which disappeared in older mice for DBP, but not for transcortin. In 7-day-old animals, no sex difference was observed for either protein, but in older animals a clear sex difference was found for transcortin. Adult males tended to have somewhat higher levels of DBP than adult females, but this difference was significant only on day 70. The variation in transcortin and DBP levels was further investigated in a large number of mouse strains. The DBP concentration did not markedly vary among strains (5.98-9.65 mumol/l in males and 5.08-8.85 mumol/l in females). Transcortin, however, showed marked strain variations, ranging from 0.72 to 2.06 mumol/l in males and from 1.02 to 4.55 mumol/l in females and there was a significant correlation (r = 0.66, n = 26, P less than 0.001) between the mean transcortin levels in males and females of different strains. Interstrain variation was much higher than intrastrain variation or variation among related strains, suggesting that the transcortin concentration is largely controlled by genetically determined factors. There was a significant correlation (r = 0.82, n = 9, P less than 0.01) between the mean corticosterone and transcortin concentrations (measured at 21.00 h).(ABSTRACT TRUNCATED AT 250 WORDS)     

云南斯氏狸殖吸虫染色体核型的研究

目的了解云南斯氏狸殖吸虫染色体核型。方法取云南斯氏狸殖吸虫成虫卵巢和睾丸,经短期细胞培养后制作染色体标本片,显微镜观察并照相。结果云南斯氏狸殖吸虫的染色体核型为n=11和2n=22。结论云南斯氏狸殖吸虫染色体核型与四川斯氏狸殖吸虫相同。     

人类、家畜及野生动物体内鞭虫卵形态特征之比较研究(英文)

鞭虫具有世界性之分布,多流行于热带、亚热带及卫生状况较差之地区。虫体形体似鞭状,故称鞭虫。虫体呈棕黄色后端较粗,长3～5cm,雌虫较长。虫体后端含有肠道及生殖器官。大便中桶状虫卵为诊断之特征。成虫及虫卵之形体与人体者类似,惟家畜及野生动物则具有不同之特征。雌虫(35～55cm)较雄虫(30～45cm)长。一雌虫每天可产3000～70000虫卵。卵两端各有一凸出塞子,形体柠檬。大小为50-54×22-23μm。     

Variability of the intestinal immunoglobulin E response of rats to infection with Trichinella spiralis, Heligmosomoides polygyrus or Nippostrongylus brasiliensis

Total intestinal IgE level increased in rats infected with Trichinella spiralis or Heligmosomoides polygyrus (peak levels of 2.6 microg and 3.7 microg, respectively), but not in rats infected with Nippostrongylus brasiliensis. Intestinal implantation of young adult N. brasiliensis did not stimulate an intestinal immunoglobulin (Ig)E response, suggesting that mucosal penetration may be required for local intestinal IgE responses in rats. During a T. spiralis infection, total IgE levels in the intestinal lumen were consistently higher in LEWIS and LOU rats (rat strains that eliminate T. spiralis worms earlier in the infection) than in PVG, AO and WKA/H strain rats. There was no correlation in either the total level of serum IgE and IgA, or of intestinal IgA with differences between strains in the rate of worm elimination from the gut. Furthermore, the intestinal IgE immunoprecipitated from LEWIS rats 12 days after infection reacted with T. spiralis adult worm metabolic antigens, while intestinal IgE from PVG rats only became reactive with adult worm metabolic antigens from 14 days after infection. These data emphasize the significance of the intestinal IgE response and its unique features by comparison with serum IgE and IgA or intestinal IgA.     

血吸虫对吡喹酮抗药性的研究ⅩⅦ 日本血吸虫吡喹酮抗性株在小鼠体内的生物学特性

目的实验观察日本血吸虫吡喹酮抗性株在终宿主小鼠体内的生物学特性,探索日本血吸虫吡喹酮抗性株对终宿主的致病力及传播强度。方法分别采用日本血吸虫江苏吡喹酮敏感株和抗性株、湖南吡喹酮敏感株和抗性株尾蚴定量感染小鼠,建立小鼠-钉螺-小鼠生活史循环,观察比较各虫株的虫卵开放前期、产卵量及虫卵分布、对终宿主的易感性、虫体生长发育等生物学特性。结果日本血吸虫江苏吡喹酮敏感株和抗性株虫卵开放前期分别为36.1 d和36.8d(t=0.907,P=0.372),粪便中虫卵数分别为14.6只/100 mg和21.2只/100 mg(t=2.946,P=0.007),回收成虫数分别为20.5条/鼠和25.1条/鼠(t=2.128,P=0.042),组织中虫卵数分别为31 303只/对成虫和38 594只/对成虫(t=2.185,P=0.040)、肝脏虫卵数分别为14 810只/对成虫和19 715只/对成虫(t=2.934,P=0.007),肠组织中虫卵数分别为16 493只/对成虫和18 879只/对成虫(t=1.044,P=0.309);江苏敏感株和抗性株雌雄合抱虫体长度(t=0.328,P=0.744)、雌虫体长(t=0.386,P=0.701)及雄虫体长(t=0.332,P=0.741)差异均无统计学意义。日本血吸虫湖南吡喹酮敏感株和抗性株虫卵开放前期分别为35.5 d和35.6 d(t=0.169,P=0.867),粪便中虫卵数分别为13.3只/100 mg和18.9只/100 mg(t=3.622,P=0.001),回收成虫数分别为17.6条/鼠和25.1条/鼠(t=3.153,P=0.004),组织中虫卵数分别为30 932只/对成虫和53 903只/对成虫(t=3.865,P=0.001),肝脏虫卵数分别为12 307只/对成虫和26 363只/对成虫(t=4.388,P0.01),肠组织中虫卵数分别为18 625个/对成虫和27 541个/对成虫(t=2.679,P=0.012);湖南敏感株和抗性株雌雄合抱虫体长度(t=0.853,P=0.397)、雌虫体长(t=0.573,P=0.569)及雄虫体长(t=0.742,P=0.461)差异均无统计学意义。结论日本血吸虫吡喹酮抗性株产卵量和肝组织虫卵沉积量均明显高于敏感株,提示其对终宿主的致病性更强;抗性株感染鼠粪便中排出虫卵数明显多于敏感株,提示抗性株的传播能量高于敏感株。