Cytoplasmic and axoplasmic density variations in relation to hyperactivity

Summary The density of the cytoplasm and axoplasm of the anterior horn cell in rats was determined by X-ray microradiography. The average density of the cytoplasm of more than 400 cells from control rats was 0.31 g/³, while that of over 600 cells from rats fed IDPN (- iminodipropionitrile) was 0.43 g/³.Hyperactivity developed during the first 5 weeks and was associated with a gradual increase in cytoplasmic density to 0.51 g/³.At 6 weeks there was a drop in density to 0.36 g/³ which coincided with the appearance of axonal balloons having a density of 0.17 g/³.During the 7–12th week on the diet, the cytoplasmic density showed a gradual increase to 0.59 g/³ and the balloons to 0.29 g/³.The volume of the nerve cells remained fairly constant. The density increases were discussed in relation to hypertrophy, dystrophy, and hyperactivity.

---

Zusammenfassung Die Dichte des Cytoplasmas und Axoplasmas der Vorderhornzellen von Ratten wurde durch Röntgenmikroradiographie bestimmt. Die mittlere Dichte des Cytoplasmas von mehr als 400 Zellen der Kontrollratten war 0,31 g/³, während die mittlere Dichte von mehr als 600 Zellen der Ratten, die mit IDPN (- iminodipropionitrile) gefüttert waren, 0,43 g/³ war.Hyperaktivität entwickelte sich während der ersten 5 Wochen und war mit einer progressiven Zunahme der Cytoplasmadichte bis auf 0,51 g/³ verbunden.Nach 6 Wochen sank die Dichte auf 0,36 g/³. Diese Tatsache traf mit dem Auftreten der Axonauftreibungen zusammen, die eine Dichte von 0,17 g/³ hatten.Nach 7–12 Wochen zeigte die Cytoplasmadichte eine progressive Zunahme auf 0,59 g/³ und die der Auftreibungen eine Zunahme auf 0,29 g/³.Das Volumen der Nervenzellen blieb ziemlich konstant.Die möglichen Zusammenhänge zwischen Zunahme der Dichte, Hypertrophie, Dystrophie und Hyperaktivität werden dargestellt.

---

---

Supported by U. S. Public Health Grant NB 1305.     

Presynaptic effects of gamma-aminobutyric acid on norepinephrine release and uptake in rat pineal gland

Summary The effect of -aminobutyric acid (GABA) on pineal norepinephrine (NE) release was examined in vitro in the rat pineal gland. Exposure of pineal expiants previously loaded with³H-NE to 1–100 M GABA caused a dosedependent decrease of³H-NE release triggered by 60 mM K⁺, with a threshold GABA concentration of 1 M and IC₅₀ of about 10 M. The inhibitory effect of GABA was mimicked by the type B GABA agonist baclofen, displaying a similar dose-response relationship as GABA. The type A GABA agonist muscimol increased depolarization-induced³H-NE release, while the co-incubation with GABA and the type A receptor antagonist bicuculline augmented significantly GABA's depressive effect on³H-NE release. Bicuculline alone brought about a significant decrease of³H-NE release. Neither GABA, nor baclofen, muscimol or bicuculline, modified the spontaneous pineal³H-NE efflux. Assessment of³H-NE uptake at a low NE concentration (0.5 M) indicated that GABA decreased it in a dose-dependent manner (IC₅₀=100 M) through an effect blocked by bicuculline and mimicked by muscimol but not by baclofen; at a 5 M-³H-NE concentration a bicuculline-sensitive GABA augmentation of uptake was found. A kinetic analysis study of the pineal NE uptake process indicated that GABA augmented both Vmax and Km of transmitter uptake. These results indicate that GABA may be a significant regulatory signal for rat pineal sympathetic synapses.     

A special type of senile plaque,possibly an initial stage

Summary It is customary to distinguish primitive, classic and compact (burned out) senile plaques in Alzheimer's disease and senile dementia of the Alzheimer type (SDAT). Primitive plaques are characterized by altered neurites without accumulation of amyloid, classic plaques by an amyloid core surrounded by altered neurites and compact plaques by amyloid without pathological neurites. Here we describe a further type of plaque in which no amyloid or obviously altered neurites could be found by light microscopy with appropriate stains. This type of plaque was found mainly in the lateral entorhinal region and could be recognized by a slightly more intense staining and an altered texture of the neuropil in a spherical area having the same size as an early or mature plaque (100–150 m in diameter). In nonserial paraffin sections (3–4 m thick), a dark, silverpositive cell measuring 10–12 m in diameter was found in the center of 49 out of 400 such plaques (about 12%), which is the expected frequency if one assumes that every plaque contains such a cell and measures itself about 125 m. In fact, the reconstruction of 15 plaques (from four different patients) by means of serial sections demonstrated the presence of a central cell in each of them suggesting that this cell is an essential component of this plaque type. The central cell did not react with antibodies against cells of the mononuclear phagocyte lineage, such as alpha-1-antichymotrypsin, alpha-1-antitrypsin, leucocyte common antigen and lysozyme. However, it was consistently stained using the lectinRicinus communis agglutinin-1 (RCA-1), which is known to stain specifically microglia in the normal human brain. We assume that the type of plaque we describe might correspond to a very early step of plaque development and that they are possibly formed even before the primitive plaques mentioned above. We further suggest that such very early lesions might be caused by abnormal activity of microglial cells.     

Untersuchungen des Plasma-Cortisols bei psychiatrischen Krankheitsgruppen

Zusammenfassung Bei 23 endogen Depressiven, 24 Schizophrenen, 15 Manikern, 23 depressiven Neurotikern, 23 nichtdepressiven Neurotikern und 29 nichtpsychiatrischen Patienten als Kontrollen wurde mit der Radioimmunassay-Methode der Plasma-Cortisol-Gehalt am Morgen (7.30 Uhr) und am Abend (19.30 Uhr) bestimmt. Der multiple Vergleich der Gruppenmittelwerte ergab folgende signifikante Unterschiede: In den Morgenwerten liegen die nichtdepressiven Neurotiker (¯x=16,6 g/100 ml), endogen Depressiven (¯x =14,8 g/100 ml) und depressiven Neurotiker (¯x=14,4 g/100 ml) höher als die Kontrollen (¯x=10,0 g/100 ml). In den Abendwerten liegen die nichtdepressiven Neurotiker (¯x=8,8 g/100 ml), Maniker (¯x=6,4 g/100 ml) und endogen Depressiven (¯x=7,0 g/100 ml) höher als die Kontrollen (¯x=3,6 g/ 100 ml), die nichtdepressiven Neurotiker (¯x=8,8 g/100 ml) außerdem höher als die depressiven Neurotiker (¯x=4,9 g/100 ml) und Schizophrenen (¯x= 4,4 g/100 ml).Die Ergebnisse werden mit der Literatur verglichen. Die Interpretation der Befunde ist problematisch: Neben krankheitsunspezifischen Streßfaktoren scheinen auch — zumindest bei endogener Depression — krankheitsspezifische Faktoren eine Rolle zu spielen.     

Die spektralphotometrische Analyse des Liquor cerebrospinalis im UV-Licht

Zusammenfassung Die spektralphotometrische Bestimmung der Absorptionskurve des natürlichen Liquors im UV-Licht gestattet in exakt reproduzierbarer Weise normale und pathologische Liquores zu unterscheiden. Dies ist namentlich bei Verlaufskontrollen von Bedeutung. Ein konstantes Absorptionsmaximum (A) findet sich im kurzwelligen Bereich bis 240 m und ist zur quantitativen Eiweißbestimmung mit einer für die klinischen Bedürfnisse hinreichenden Genauigkeit geeignet. Eine von den quantitativen Eiweißwerten unabhängige Absorptionsbande ( A) hat ihr Maximum bei allen normalen und den meisten pathologischen Liquors bei 266 m Eine Verschiebung nach 275 m kommt vereinzelt bei pathologischen Liquores vor.Eine starke A mit einem Durchlaßgrad von weniger als 30% spricht auch bei im übrigen normalen Liquorwerten für pathologische Veränderungen.Das mittels Perchlorsäure gefällte Liquoreiweiß zeigt in alkalischer Lösung ein dem Grade nach von dem Eiweißgehalt abhängiges Absorptionsmaximum bei 215 m und eine flache Absorptionsbande zwischen 275–285 m.     

Effect of tryptophan metabolites on activity of the epileptogenic focus in the frog hippocampus

Summary In frogs with the epileptogenic foci made by injection of penicilline (1000 U in 0.4 mcl) in the primordial hippocampus it was shown that preliminary administration of two kynurenines quinolinic acid (0,1g) and d,l-kynurenine (1g), in the foci region, and their injection in the functioning epileptogenic foci led to a strong increase of the interictal epileptiform discharges and of the electrographic correlates of fits on the EEG. Anthranilic acid (0.1, 1.0 and S.0g) did not influence the activity of epileptogenic foci. Serotonin (1g) and 5-methoxytryptamine (1g) essentially decreased it. Provocating effect of kynurenines on neurons in epileptogenic foci is supposed to play a certain role in pathogenesis of epilepsy.     

Effect of parasympathetic blockade on the signalaveraged electrocardiogram

Low parasympathetic activity is associated with late potentials detected at a noise level of 0.4 V in a signal-averaged electrocardiogram (SAECG) following myocardial infarction. In contrast, at a noise level of 0.2 V, lowering parasympathetic activity influences late potential parameters in the opposite direction in healthy subjects. The aim of this study was to estimate the relationship between parasympathetic activity and the SAECG obtained at noise levels of 0.4 and 0.2 V in healthy subjects. Two SAECG recordings in 10 healthy subjects were obtained at noise levels of 0.2 and 0.4 V before and after parasympathetic blockade using atropine (1 mg). Signal-averaged QRS duration (SA-QRS), late potential duration (LPD) defined as duration of terminal signals below 40 V, and root mean square voltage of the terminal 40 ms of the averaged QRS (RMS₄₀) were measured. At a noise level of 0.2 V SA-QRS reduced from 124±14 to 114±17 ms (P=0.008), LPD from 37±10 to 28±14 ms (P=0.01), and RMS₄₀ increased from 26±22 to 41±25 V (P=0.006) during parasympathetic blockade compared to baseline values. At a noise level of 0.4 V the SA-QRS (115±15 ms) and LPD (29±11 ms) were lower and the RMS₄₀ (37±23 V) was higher compared to the noise level 0.2 V, and no systematic alterations of the three variables were found during parasympathetic blockade. The parasympathetic nervous system may induce a very low-amplitude late potential in the SAECG. The data suggest that parasympathetic activity and a low noise level may lead to a false late potential-positive SAECG in low arrhythmia risk subjects. Therefore, we recommend the use of a noise level of 0.4 V or identification of high arrhythmia risk patients by late potentialand low parasympathetic activity.     

Fluctuating parkinsonism: a pilot study of single afternoon dose of levodopa methyl ester

Thirty-four patients with idiopathic fluctuating Parkinson's disease and early afternoon delayed on or severely resistant off periods, in spite of long-term antiparkinsonian therapy, were studied. The first afternoon levodopa administration was substituted with an equimolar dosage of the liquid formulation levodopa methyl ester (LDME). The major end-points for efficacy were latency to on and duration of on periods. The patients were divided into five subgroups according to their baseline treatment and they were evaluated monthly for 6 months using the Unified Parkinson's Disease Rating Scale. The patients completed weekly self-evaluation using an on-off chart. LDME was well tolerated by all the patients. A statistically significant reduction in latency to on was observed in all patients. The clinical effect of LDME remained stable during the treatment period (repeat measures ANOVA). The more rapid clinical effect of LDME and its stable and predictable antiparkinsonian activity represents a new and useful approach for treating patients with complicated Parkinson's disease.     

Deposition of β/A4 protein along neuronal plasma membranes in diffuse senile plaques

Summary The origin of the extracellular -amyloid protein (/A4) found in senile plaques and the cellular mechanisms responsible for its deposition in cerebral tissues are still an unresolved issue in Alzheimer's disease. In this study we analyzed in detail the distribution of various epitopes of /A4 in relation to local cellular elements in diffuse plaques of the hippocampal region. We also correlated our findings with the presence and distribution of non-/A4 epitopes of the amyloid precursor protein (APP) and with synaptophysin immunoreactivity in the cortical neuropil. Discontinuous /A4-immunoreactive deposits were found along dendrites, and around the soma of neurons included in the plaques. Furthermore, increased synaptophysin reactivity with slightly dilated synaptophysin-immunolabeled presynaptic terminals were found in diffuse plaques. APP epitopes could not be found in diffuse plaques. However, some of the APP antibodies, mainly those to the C-terminal portion of APP, and antibodies to /A4 recognized clusters of flat vesicular profiles (0.6–1.4 m in width and 2–3 m in length) in the neuropil of cortical areas where plaques had developed. Our findings are compatible with a neuronal origin of /A4 in diffuse plaques and with a primary release of /A4 at synaptic sites along the immunostained neurites. They also suggest that diffuse plaques might be preceded by minute lesions of the neuropil where /A4 is not yet released from the precursor molecule.     

Cerebrovascular permeability to horseradish peroxidase in hypertensive rats: effects of unilateral locus ceruleus lesion

Summary Unilateral locus ceruleus lesion enhances leakage of radioiodinated human serum albumin into the ipsilateral cerebral cortex of rats with norepinephrine-induced hypertension. This ultrastructural study was undertaken, to determine the mechanism by which this permeability alteration occurs, using horseradish peroxidase (HRP) as a tracer. Unilateral locus ceruleus lesion was produced in male Wistar-Furth rats by stereotaxic microinfusion of 5 g of 6-hydroxydopamine. Two weeks later, rats were injected with HRP intravenously and acute hypertension was induced in awake rats by an intravenous infusion of norepinephrine (6 g), epinephrine (6 g) or angiotensin amide (12 g) given over a 2-min period. Thirty seconds later, the rats were perfused with fixative under deep anesthesia and their brains were sliced and processed for demonstration of HRP reaction product. Leakage of HRP occurred in both cerebral hemispheres in response to hypertension induced by the three pressor agents, but the leakage was greater on the lesioned side in response to epinephrine and norepinephrine, while in the case of angiotensin-induced hypertension side-to-side differences in permeability alterations were not observed. In both cerebral hemispheres increased permeability affected mainly arterioles, which showed enhanced pinocytosis as the principal mechanism of HRP extravasation.Supported by Heart and Stroke Foundation of Ontario Grant 2–6 (to S. N.) and by United States Public Health Service Grant NS-18150 (to S. I. H.)     

Equol and other compounds from bovine urine as monoamine oxidase inhibitors

Summary Equol, its methylated derivative, and a carbazole, all isolated from bovine urine, are relatively potent inhibitors of monoamine oxidase with IC₅₀ values of 158, 28, and 16M respectively (using 83M tyramine as substrate). The probable dietary origin of these compounds suggests that natural monoamine oxidase inhibitors may be more widespread than had previously been suspected.     

Liquorkomplement und Multiple Sklerose

Zusammenfassung 1. In 239 Liquores, darunter 47 MS-Fälle und 91 normale Kontrollen, wurden Komplement und Komplementfaktoren (C₁, C₂, C₃ und C₄) bestimmt.2. Im normalen Liquor ist in der Regel keine Gesamtkomplementaktivität vorhanden. Dagegen ist die C₁- wie C₄-Aktivität praktisch immer und C₂-sowie C₃-Aktivität in über der Hälfte der Fälle zu finden. Das Komplementmuster im Liquor ist daher im Gegensatz zum Serum unvollständig.3. Bei erhöhtem Eiweiß zeigt der Liquor dagegen häufig Gesamtkomplementaktivität. Je höher das Liquoreiweiß ist, um so höher ist der Gehalt an C₂, C₃ und Gesamtkomplement. Diese Beziehungen zwischen Gesamteiweiß und Komplementaktivität gelten für normalen wie pathologischen Liquor einschließlich der MS-Fälle.4. Im MS-Liquor sind C₂, C₃ und Gesamtkomplement seltener zu finden als bei den Kontrollen. Bei den MS-Patienten ist C₂ und C₃ im akuten Schub herabgesetzt. C₃ nimmt im Verlauf der Erkrankung wahrscheinlich ab.5. Mit Antikomplementserum wurde _1C-Globulin, ein Teilfaktor von C₃, im Liquor von 55 MS-Patienten und 42 Kontrollen bestimmt. Es besteht kein Unterschied zwischen MS und Kontrolliquor. Auch bei akut entzündlicher MS ist _1C nicht vermindert.6. In der Diskussion wird auf widersprechende eigene Befunde über _1C-Inaktivierung im Serum während der akut entzündlichen MS-Phase hingewiesen.

---

Summary 1. Complement and complement factors (C₁, C₂, C₃ and C₄) were determined in 239 specimes of cerebrospinal fluid (CSF) including 47 cases of Multiple Sclerosis (MS) and 91 normal controls.2. In general, total complement activity is absent in normal specimens while that of C₁ and C₄ can be found practically always and that of C₂ and C₃ in more than half of the cases. Therefore, the pattern of complements in the CSF is incomplete as opposed to that of serum.3. In contrast, samples with increased protein content frequently yield total complement activity. The higher the protein content of CSF the higher the content of C₂, C₃ and total complement. This relationship between amount of total protein and complement activity applies both to normal and pathological CSF specimens including those from MS.4. In cerebrospinal fluid from patients with MS, C₂, C₃ and total complement are found less frequently than in that from controls. C₂ and C₃ are diminished in patients with an acute exacerbation of MS. C₃ decreases probably in the course of the disease.5. _1C-globulin, a component of C₃, was determined with anticomplement sera in specimens from 55 patients with MS and from 42 controls. There is no difference between CSF of MS and controls. Even in acutely inflammatory cases of MS, _1C is not diminished.6. Discussing his results the author points out discrepancies concerning the nactivation of _1C in serum during acutely inflammatory episodes of MS.

---

---

Mit Unterstützung durch die Deutsche Forschungsgemeinschaft.     

Single drug therapy for intractable epilepsy

Summary Single drug therapy with either phenytoin or primidone resulted in complete seizure control in 11 of 35 patients (31%) referred to an epilepsy clinic for treatment of uncontrolled chronic epilepsy with complex-partial seizures. Complete seizure control was associated with an increase in the mean plasma concentrations from 14 g/ml to 23 g/ml phenytoin and from 34 g/ml to 40 g/ml phenobarbitone with no change in the antiepileptic drug. Insufficiently low plasma concentrations of less than 11 g/ml phenytoin or phenobarbitone were measured at the first visit in 14 patients (40%). Non-compliance was admitted by eight patients (23%). Optimum single drug therapy is of considerable clinical value in intractable epilepsy with complex-partial seizures.

---

Zusammenfassung Eine Monotherapie mit Phenytoin oder Primidon führte zur Anfallsfreiheit bei 11 von 35 Patienten (31%), die wegen schwerbehandelbarer psychomotorischer Anfälle eine Epilepsieambulanz aufsuchten. Anfallsfreiheit trat auf bei einem Anstieg der mittleren Plasmakonzentration von 14 g/ml auf 23 g/ml Phenytoin und von 34 g/ml auf 40 g/ml Phenobarbital. Ein Wechsel der Medikamente war nicht notwendig. Während der ersten Untersuchung wurden bei 14 Patienten (40%) zu niedrige Plasmakonzentrationen von weniger als 11 g/ml Phenytoin oder Phenobarbital gefunden. 8 Patienten (23%) gaben eine unregelmäßige Einnahme der Medikamente (non-compliance) zu. Eine konsequente Monotherapie ist von klinischem Wert für die Behandlung von schwerbehandelbaren Epilepsien mit psychomotorischen Anfällen.

     

Morning administration of melatonin antagonizes the antigonadotrophic effect of afternoon injections of melatonin, 5-methoxytryptophol and arginine vasotocin in intact mice

Summary In intact prepubertal mice kept under a long photoperiod subcutaneous afternoon (4.30 p.m.) injections (20 g) but not morning (9.30 a.m.) injections (100 g) of melatonin and 5-methoxytryptophol elicited antigonadotrophic effects. A morning dose (100 g) of methoxytryptophol abolished the antigonadotrophic effects of afternoon injections of methoxytryptophol. Similarly the antigonadotrophic effects of afternoon injections of melatonin, 5-methoxytryptophol and arginine vasotocin could be partially destroyed by 100 g and completely abolished by 1 mg melatonin administered in the morning.     

Spheroids and altered axons in the spinal gray matter of the normal cat

Summary In our recent ultrastructural studies on synapses of the nucleus dorsalis, central cervical nucleus, and anterior horn of the spinal cord of the normal cat we happened to find spheroids and several types of axonal alterations. These spheroids were up to 39 m in diameter. They were found in myelinated and unmyelinated terminal axons and at the node of Ranvier and showed two different types of internal structure. One type was large and composed of spirally arranged neurofilaments and mitochondria in increased quantity, although the mean population density of mitochondria was not high being 1.4/m² as compared to the normal value 2.0/m². Another type was smaller and consisted of small mitochondria and dense bodies which were increased in number: their mean population densities were 4.5/m² and 1.9/m², respectively.At present, the rare occurrence of spheroids and atypical axonal alterations makes it difficult to determine the origin of affected axons, although some of them presumably arise from primary afferents.     

Golgi-like demonstration of “dark” neurons with an argyrophil III method for experimental neuropathology

Summary A silver method is proposed for the selective, well-contrasted and reproducible demonstration of dark neurons in frozen, vibratome and paraffin sections cut at a thickness of 5 to 200 m from aldehyde-fixed brains. The Golgi-like staining of the dendrites enables asorting of dark neurons according to characteristic neuron classifications. The staining procedure includes an esterification with 1-propanol, a treatment with diluted acetic acid and development. The esterification strongly increases the argyrophilia of both dark neurons and mitochondria. Unwanted co-staining of mitochondria is suppressed by the acetic acid treatment, while a special developer is used to render the staining controllable. The applicability of the method to experimental neuropathology is demonstrated by Golgi-like staining of dark neurons in rat brains exposed, before transcardial perfusion-fixation and delayed autopsy, to various pathological conditions including ischemia, hypoglycemia, trauma, status epilepticus, deafferentation and poisoning with kainic acid, colchicine and sodium azide, respectively.     

I. c. v. dopamine fails to alter adrenomedullary function in rats

Summary Intracerebroventricularly (i.c.v.) administered dopamine (50–400 g/ kg, 30 min; 400 g/kg, 2 and 4 h) did not induce statistically significant changes in heart adrenaline (presumed to represent the adrenaline release from the adrenals). Only the highest dose (400 g/kg, 30 min) brought about significant increase in adrenal dopamine (reflecting the catecholamine synthesis), accompanied by increase in heart dopamine, indicating leakage of dopamine into the peripheral circulation.The results did not support involvement of the central dopamine receptors in the short-term control of the adrenomedullary function in rats.     

Immunoglobulin heavy chain gene associations in myasthenia gravis: new evidence for disease heterogeneity

Summary Susceptibility to myasthenia gravis (MG) is known to involve genes residing in the major histocompatibility complex class I and II regions (HLA-B8 and DR3). Immunoglobulin heavy chain constant region (IgCH) allotypes have also shown some associations with MG. We have used restriction fragment length polymorphism analysis with probes to the IgCH switch (S) regions and 1 and the downstream marker D14S1 to investigate 189 Caucasoid patients with well-defined MG. A highly significant increase in the frequency of the 2.6 kilobase (kb) S homozygous genotype and the 2.6 kb S allele was found in patients with disease onset after the age of 40 years (late onset) compared with normal controls (P<0.00075 andP<0.025 respectively). No association was found at the S1 or D14S1 loci. In patients with an associated thymoma there was a moderate increase in the frequency of the 2.6 kb S and 7.4 kb S1 genotypes. These results independently support the previous separation of the late-onset subgroup. Finally, the stronger associations at S rather than at the downstream Sl, Gm and D14S1 loci suggest that the genes predisposing to MG are located within the variable region of the Ig heavy chain loci.     

Dose response relationship between plasma ACTH and cortisol after the infusion of ACTH1–24

Summary The authors examined the dose response relationship between plasma ACTH and cortisol concentrations after the administration of various doses of ACTH_1–24 (0.025 g, 0.125 g, 0.25 g, 1 g, 250 g) in dexamethasone-suppressed normal volunteers. A logarithmic dose-response relationship between the dose of ACTH administered and plasma cortisol concentration was found. Although there was considerable variability in plasma ACTH concentrations, there was, however, a definite correlation between area under the curve for ACTH and area under the curve for cortisol after the various doses of ACTH.     

Mental Hygiene of the Special Schools Teachers in Kerman,Iran

The goal of this research is to identify and study the mental hygiene and its related factors (individual, family, organizational) in the Kerman special schools teachers. 266 teachers of the special schools of the cities of the province Kerman were chosen as the research sample. The necessary data were obtained by questionnaire which its validity and reliability were determined. The statistical analysis of the research findings (by Spearman coefficient test) Man-Whithey and Kruskal wallis tests showed that there was a positive and meaningful relationship between Level of education, service of record the number of the members of teachers family, income, dwelling-place, economic power, acceptance of the teachers job in their family, leadership style, suitable educational possibilities, suitable educational space, job satisfaction with the mental hygiene of the teachers. The results of the research also showed that the Kerman special schools teachers enjoyed a relatively desirable mental hygiene.