Clinical Significance of VEGF-C and VEGFR-3 Expression in Non-small Cell Lung Cancer

The relationship between vascular endothelial growth factor-C (VEGF-C)/vascular en-dothelial growth factor receptor 3 (VEGFR-3) expression and clinicopathologic features of the patients with non-small cell lung cancer (NSCLC) was investigated. The expression of VEGF-C and VEGFR-3 was assessed in 65 patients with NSCLC by immunohistochemistry. The significance of VEGF-C and VEGFR-3 expression was analyzed statistically. The results showed that VEGF-C and VEGFR-3 were highly expressed in cytoplasm and membrane in lung cancer tissues with the positive rate being 55.4 % and 52.3 % respectively, while there was no expression in the normal lung tissues. The expression of VEGF-C was significantly increased in adenocacinoma as compared to other types of NSCLC (P<0.05). The VEGFR-3 expression was closely related with lymph node metastasis (P<0.01) and TNM stage (P<0.05). There was a positive correlation between the VEGF-C and VEGFR-3 expression in NSCLC patients (r=0.658, P<0.01). It is suggested that VEGFR-3 plays an important role in the lymphatic metastasis of NSCLC. The interaction between VEGF-C and VEGFR-3 may be deeply involved in the mechanism of lung cancer metastasis.     

中性粒细胞明胶酶相关蛋白及基质金属蛋白酶10在非小细胞肺癌中的表达及临床意义

目的 观察中性粒细胞明胶酶相关蛋白(NGAL)和基质金属蛋白酶10(MMP-10)在非小细胞肺癌(NSCLC)中的表达,探讨其与NSCLC浸润、转移的关系.方法 采用免疫组织化学SP法检测70例非小细胞肺癌组织及30例癌旁肺组织标本中NGAL和MMP-10的表达,并分析它们的表达与相关临床参数的关系.结果 NSCLC组织中NGAL和MMP-10的表达分别为67.1%、70.0%,并与肿瘤直径、TNM分期、淋巴结转移明显相关(P<0.05),而与患者性别、年龄无关.NGAL和MMP-10的表达呈正相关(r=0.376,P=0.001).结论 NSCLC组织中NGAL和MMP-10表达明显增加.NGAL和MMP-10可能在NSCLC的发生、发展中起一定作用. Abstract： Objective To observe the expression of neutrophil gelatinase associated lipocalin (NGAL) and matrix metalloproteinase 10(MMP10) in human non-small cell lung cancer(NSCLC) and to investigate their correlations with the infiltration and metastasis of NSCLC.Methods Immunohistochemistry method was applied to investigate the expression of NGAL and MMP-10 in specimens of 70 NSCLC tissues and 30 paracancerous tissues.The relationship between the expression of NGAL and MMP-10 in the NSCLC tissue and related clinical parameters were analyzed. Results The positive expression rates of NGAL and MMP-10 in NSCLC tissues were 67.1% and 70.0%,respectively. The expression of NGAL and MMP-10 in NSCLC tissues was correlated with the diameter of the mass, the pathological grading, the lymph node metastasis, but not correlated with sex, age and smoking. There was a positive correlation between NGAL and MMP-10 expression in lung squamous cell cancer tissues (r=0.376, P=0.001).Conclusions Higher expression of NGAL and MMP-10 may play an important role in carcinogenesis and development of NSCLC.     

Expression and Clinical Implication of HIF-la and VEGF-C in Non-small Cell Lung Cancer

To study the expression and implication of HIF-la and VEGF-C in non-small cell lung cancer (NSCLC) and its relationship with clinical pathological features of NSCLC, immunohistochemical SP was used to detect the expression of HIF-1а and VEGF-C proteins in 48 NSCLC tissues and the same para-cancerous tissues. The positive rates of HIF-1а and VEGF-C were 70.8% (34/48) and 68.8% (33/48) respectively. The expression of HIF-la protein was detected in a significantly greater proportion in NSCLC carcinoma tissues than that in para-cancerous tissues (12.5% and 16.7%,P<0.05). The positive rates of HIF-1а and VEGF-C were correlated with lymph node metastasis and TNM stage. No relationship was found between the two factors and age, sex, pathological subtypes and histological grades. The positive rates between HIF-1а and VEGF-C were correlated (P<0.05).HIF-1а and VEGF-C were over-expressed in NSCLC. They may be involved in the carcinogenesis of NSCLC, and play an important role in invasion and metastasis of NSCLC. HIF-1а and VEGF-C work synergically in the process of NSCLC.     

Expression and Clinical Implication of HIF-1α and VEGF-C in Non-small Cell Lung Cancer

To study the expression and implication of HIF-1α and VEGF-C in non-small cell lung cancer （NSCLC） and its relationship with clinical pathological features of NSCLC, immunohisto-chemical SP was used to detect the expression of HIF-1α and VEGF-C proteins in 48 NSCLC tissues and the same para-cancerous tissues. The positive rates of HIF-1α and VEGF-C were 70.8% （34/48） and 68.8% （33/48） respectively. The expression of HIF-1α protein was detected in a significantly greater proportion in NSCLC carcinoma tissues than that in para-cancerous tissues （12.5% and 16.7%, P〈0.05）. The positive rates of HIF-1α and VEGF-C were correlated with lymph node metastasis and TNM stage. No relationship was found between the two factors and age, sex, pathological subtypes and histological grades. The positive rates between HIF-1α and VEGF-C were correlated （P〈0.05）. HIF-1α and VEGF-C were over-expressed in NSCLC. They may be involved in the carcinogenesis of NSCLC, and play an important role in invasion and metastasis of NSCLC. HIF-1α and VEGF-C work synergically in the process of NSCLC.     

EXPRESSION AND SIGNIFICANCE OF SURVIVIN mRNA IN LUNG CANCER TISSUE MICROARRAY DETECTED BY FISH

Objective To investigate the expression of Survivin mRNA in lung cancer tissue microarray (TMA) by fluorescence in situ hybridization (FISH) method, and determine the role and significance of it in lung cancer genesis and progress. Methods The expression of Survivin mRNA was detected by FISH method and TMA technology. Fifty-four cases of lung cancer and 10 cases of normal lung tissue were examined. Results Survivin mRNA was expressed in 66.7% (36/54) of lung cancer; the positive ratio of lung cancer was significantly higher than that of normal lung tissue (0/10; x2= 15.238, P ＜ 0.05). The positive ratio of Survivin mRNA was significantly higher in poor differentiated cancer (20/24, 83.3%) than moderate and well differentiated cancer (16/30, 53.3%; x2= 5.40, P ＜0.05). The positive ratio of Survivin mRNA was significantly higher in group with lymph node metastasis (27/32, 84.4%) than without lymph node metastasis (9/22, 40.9%; x2= 11.084, P ＜ 0.05). The positive ratio of Survivin mRNA was significantly higher in stage Ⅲ-Ⅳ(12/13, 92.3%) than stage Ⅰ - Ⅱ (24/41, 58.5%; x2= 5.066, P ＜ 0.05). Conclusion Survivin mRNA highly expresses in lung cancer, which is related to the progress and malignant behavior. Survivin may play a promoting role in lung cancer genesis and progress and provide a basis for estimating prognosis and treatment.     

Clinical significance of PHPT1 protein expression in lung cancer

Background In our previous studies, we found the expression of 14-kD phosphohistidine phosphatase （PHPT1） was associated with lung cancer cells migration and invasion, and PHPT1 mRNA expression level in lung cancer tissues clinically correlated with lymph node metastasis. In the present study, we aimed to further investigate the expression of PHPT1 protein in lung cancer. Methods Expression of PHPT1 protein in tissue samples from 146 lung cancers and 30 normal tissues adjacent to lung cancers was assessed using immunohistochemical method. Fisher＇s exact test was used to analyze expression patterns of PHPT1 protein in these tissue types. Meanwhile, we studied the correlation between expression of PHPT1 protein and clinicopathological features in lung cancer. Results Significantly higher expression levels of PHPT1 protein were found in lung cancer samples （53.42%） than in normal tissues adjacent to lung cancer （23.33%） （P=0.003）. Fisher＇s exact test showed that lung cancer stage positively correlated with expression of PHPT1 protein （P=-0.02）, and lung cancer samples with lymph node metastasis showed higher PHPT1 protein expression （P=-0.016） than the samples without lymph node metastasis. Conclusions The results of this study agree with findings from our previous study of PHPT1 mRNA expression in lung cancer tissues, and strongly suggest that PHPT1 protein is closely associated with the carcinogenesis and metastasis of lung cancer. Thus, therapy targeting PHPT1 （inhibition or silencing） could be potentially benefited for lung cancer patients.     

Expression of p57kip2 and cyclinE proteins in human pancreatic cancer

Objective To investigate the effects of p57kip2 and cyclinE proteins on the genesis and progression of human pancreatic cancer. Methods The expression of p57kip2 and cyclinE proteins in tumor tissues and adjacent tissues of pancreatic cancer in 32 patients was detected by SP immunohistochemical technique. Results The p57kip2 protein positive-expression rate in tumor tissues of pancreatic cancer was 46.9%, which was lower than that in adjacent pancreatic tissue (P&lt;0.05). The p57kip2 protein positive-expression correlated significantly with tumor cell differentiation (P&lt;0.05) and did not correlate significantly with lymph node metastasis (P&gt;0.05). The cyclinE positive-expression rate in tumor tissues was 68.8%, which was higher than that in adjacent pancreatic tissues (P&lt;0.05). The cyclinE positive-expression also correlated significantly with tumor cell differentiation and lymph node metastasis (P&lt;0.05). The cyclinE protein positive-expression rate in the tumor tissues of the p57kip2 protein positive-expression group was lower than that in the p57kip2 protein negative-expression group, and there were no significant correlation between the two groups (r= -0.112, P＞0.05).Conclusion Decreased expression of the p57kip2 protein and/or over-expression of the cyclinE protein may play an important role in the genesis and progression of human pancreatic cancer.     

Role of RANTES and its receptor in gastric cancer metastasis

This study examined the role of regulated upon activation normal T cell expressed and secreted(RANTES) and its receptor C-C chemokine receptor type 5(CCR5) in gastric cancer metastasis and the associated mechanism.The expression of RANTES and CCR5 was detected by using immunohistochemical staining and Western blotting in the gastric cancer tissues obtained from 60 gastric cancer patients with or without lymph node metastasis(n=30 in each).The results showed that the expression levels of RANTES and CCR5 were higher in gastric cancer with lymph node metastasis than in that without metastasis(P<0.05).The expression levels of RANTES in 30 lymph nodes with cancerous invasion were higher than in 30 normal lymph nodes(P<0.05).Chemotactic test revealed that the number of migrating gastric cancer cells(n=295.0±54.6) induced by the protein of cancer-invading lymph nodes was greater than that by the protein mixture from cancer-invading lymph nodes and RANTES antibody(n=42.5±11.6)(P<0.05).RT-PCR showed that the expression levels of the main Th1 cytokines(IL-2,γ-IFN) were lower in gastric cancer with lymph node metastasis(2.22±0.90,3.26±1.15 respectively) than in that without metastasis(3.07±1.67,4.77±1.52 respectively)(P<0.05),but the expression level of the main Th 2 cytokine(IL-10) was higher in gastric cancer with lymph nodes metastasis(6.06±2.04) than in that without metastasis(4.88±1.87)(P<0.05).It was concluded that RANTES and its receptor CCR5 may contribute to gastric cancer metastasis through influencing the balance of Th1/Th2.RANTES and CCR5 may become a marker of gastric cancer metastasis.     

转化生长因子-β1、基质金属蛋白酶组织抑制物-1、基质金属蛋白酶-1在绝经后盆腔器官脱垂患者阴道壁组织中表达的研究

目的 探讨绝经后女性盆腔器官脱垂(POP)患者阴道壁组织中转化生长因子-β1(TGF-β1)、基质金属蛋白酶组织抑制物-1(TIMP1)和基质金属蛋白酶-1(MMP1)的表达与POP的关系.方法 选取30例绝经后盆腔器官脱垂患者(POP 组),并选择同期20例非卵巢功能性肿瘤和宫颈上皮内瘤变患者作为对照(对照组),采用免疫组化SP二步法检测患者阴道壁组织中TGF-β1、TIMP1、MMP1的表达,两组患者阴道壁组织中TGF-β1、TIMP1和MMP1含量的表达以阳性区平均积分光密度为标准.结果 TGF-β1和TIMP1在POP组的表达显著低于对照组,差异有统计学意义(P<0.01).MMP1在POP组的表达显著高于对照组,差异有统计学意义(P<0.01).结论 绝经后POP的发生可能与组织中表达的TGF-β1、TIMP1减少和MMP1增多有关. Abstract： Objective To explore the relationship between transforming growth factor-β1 (TGF-β1) and tissue inhibitors of metalloproteinase-1 (TIMP1) and matrix metalloproteinase-1 (MMP1) with the occurrence of pelvic organ prolapse (POP) through analysis on TGF - β1 and TIMP1 and MMP1 in paries anterior vaginase of postmenopausal female pelvic organ prolapse. Methods The biopsy specimens in paries anterior vaginase were obtained from 50 subjects undergoing hysterectomia, tension-free vagina tape(TVT), burch procedures, who were assigned in the control, pelvic organ prolapse groups. TGF-β1 and TIMP1 and MMP1 in paries anterior vaginase was evaluated by immunohistochemical staining. Results TGF-β1 positive staining luminance and TIMP1 positive staining luminance were significantly lower in POP group than that in control group (P<0.01),MMP1 positive staining luminance was significantly higher in POP group than that in control group (P<0.01). Conclusions There is close relationship between degeneration of pelvic support construction and deficiency in TGF-β1 and TIMP1 declines in paries anterior vaginase of POP patient,and there is also close relationship between degeneration of pelvic support construction and accrescence in MMP1 of POP patient.     

增生性糖尿病视网膜病变眼内组织型纤溶酶原激活物及其抑制物的表达与血管内皮生长因子表达的相关性

目的 探讨增生性糖尿病视网膜病变(PDR)中组织型纤溶酶原激活物(t-PA)及纤溶酶原激活物抑制剂(PAI)的表达及其与血管内皮生长因子(VEGF)表达的相关性.方法 在玻璃体手术中采集PDR 35眼玻璃体,同时采集因黄斑裂孔行玻璃体手术20眼玻璃体作为对照组,采用酶联免疫吸附法检测t-PA和PAI的表达浓度,并与VEGF的表达进行相关性分析.结果 PDR眼玻璃体中VEGF、t-PA及PAI的表达浓度与对照眼玻璃体中的表达浓度比较差异有统计学意义(P＜0.01).t-PA及PAI的表达与VEGF的表达经统计学分析,差异有统计学意义(P＜0.01).结论 在PDR眼内视网膜新生血管的发生过程中不但有VEGF,还可能同时有多种生物活性物质的参与. Abstract： Objective To evaluate the correlation between the expression of tissue plasminogen activator(t -PA)and plasminogen activator inhibitor(PAI)with vascular endothelial growth factor (VEGF)expression in proliferative diabetic retinopathy(PDR).Methods Vitreous samples were taken from 35 eyes with PDR and analyzed with enzyme linked immunosorbent assay(ELISA)for the expression of VEGF, t - PA and PAI.Control samples were from 20 eyes with idiopathic macular hole and analyzed in the same way.The correlation between VEGF expression and the expression of t - PA and PAI in proliferative diabetic retinopathy were studied.Results VEGF, t - PA and PAI were significantly expressed as compared with those in control subjects(P ＜0.01).The expression of t- PA and PAI were highly correlated with that of VEGF in proliferative diabetic retinopathy(P ＜ 0.01).Conclusions It is suggest that a number of bioactive substances may be involved in the pathogenesis of angiogenesis in PDR.The expression of t - PA may beupregulated by the activation of VEGF, which may further facilitate the process of angiogenesis in PDR.Meanwhile, the correlated expression of PAI may suggest the presence of an endogenous angiogenesis mechanism accompanying the activities of VEGF.     

国产长春瑞滨联合卡铂治疗晚期非小细胞肺癌的临床研究

目的 观察国产长春瑞滨(NVB)联合卡铂(CBP)治疗非小细胞肺癌(NSCLC)的疗效和毒性反应.方法 NVB 25mg/m2 静脉滴注第1、8天, CBP 300 mg/m2第1天,21～28 d为1个周期,完成2～6个周期.结果 45例患者中完全缓解(CR)5例,部分缓解(PR)119例,稳定(SD)10例,进展(PD)11例,总有效率(CR+PR)为53.3%.NVB主要不良反应为白细胞下降,其中Ⅲ、Ⅳ度为60%.结论 NVB加CBP方案可以作为NSCLC患者较理想的治疗方案,NVB加CBP方案具有较好的疗效和耐受性,可提高患者的生活质量. Abstract： Objective To observe the efficacy and toxicity combined chemotherapy of NVB and CBP in treatment of non-small cell lung cancer(NSCLC).Methods NVB 25 mg/m2 iv. for day 1and 8,CBP 300 mg/m2 for day 1,the treatment was recycled every 21-28 days and finished 2-6 recycles. Results Complete response was achieved in 5 cases, partial response in 19 cases,stable in 10 cases,progression in 11 cases.The overall response rate was 53.3%.Leukopenia which was the most frequent toxicity were present in 73.3%, and the grades 3,grade 4 leukopnia were present in 60%.Conclusions Combination chemotherapy of NVB and CBP is well tolerated and effective in the treatment of NSCLC and can improve quality of life(QOL).     

非小细胞肺癌患者血浆D-二聚体检测的临床意义

目的 探讨非小细胞肺癌(NSCLC)患者血浆D-二聚体检测的临床意义.方法 采用免疫比浊法检测58例非小细胞肺癌患者和19例健康体检者血浆D-二聚体水平,对肺癌患者血浆D-二聚体水平与癌症病理类型以及疾病分期关系进行分析.结果 非小细胞肺癌患者血浆D-二聚体水平[(2.40±2.07)μg/ml]较健康对照组[(0.28±0.09)μg/ml]明显升高(P＜0.01),其升高程度与肺癌临床分期有关,与癌症病理类型、是否咯血无关.78%(45/58)非小细胞肺癌患者有血浆D-二聚体水平升高与41%(24/58)患者血清癌胚抗原升高比较差异有统计学意义(P＜0.01).结论 非小细胞肺癌患者血浆D-二聚体水平升高,病情越重患者血浆D-二聚体水平越高,提示非小细胞肺癌患者存在血液高凝状态.因此,可为非小细胞肺癌患者病情判断及是否给予抗凝治疗提供参考. Abstract： Objective To investigate the clinical significance of plasma D-dimer level detection in patients with non-small cell lung cancer(NSCLC). Methods The plasma D-dimer levels of 58 NSCLC patients and 19 healthy controls were detected by immunol-nephelometry.The relationship between plasma D-dimer level and the pathological type,clinical stage of NSCLC patients were analyzed.Results The plasma D-dimer level of NSCLC patients [(2.40±2.07)μg/ml]were significantly higher, compared with the control group[(0.28±0.09)μg/ml],P＜0.01, and the plasma D-dimer level had positive correlation with clinical stage of NSCLC,but had no relation with sex,hemoptysis and pathological type. Conclusions The D-dimer levels of NSCLC patients are higher,and increases in accordance with severity of the disease,which indicated that NSCLC patients have hypercoagulable state.So the plasma D-dimer levels can be useful for evaluating severity of NSCLC.     

术中置药并术后胸腔灌注治疗Ⅲa期非小细胞肺癌的疗效观察

目的 探讨Ⅲa期非小细胞肺癌的有效治疗方法.方法 将53例Ⅲa期非小细胞肺癌患者分为观察组(26例)和对照组(27例).观察组术中用吡柔比星(THP)10 mg、环磷酰胺(CTX)200 mg、卡铂(CBP)50 mg置入清扫淋巴结处,以及术后行胸腔灌注吡柔比星50 mg、环磷酰胺600 mg、卡铂水针300 mg、α-干扰素500万U.两组均于手术后第3周采用紫杉醇(PTX)+THP+CBP/顺铂(DDP)或吉西他滨(GEM)+CBP/DDP及PTX+奥沙利铂(L-OHP)或异长春花碱(NVB)+L-OHP等多种方案交替静脉联合化疗,4周为一疗程,共4-6个疗程.随访3年进行疗效比较.结果 观察组1、2、3年生存率分别为88.4%、76.9%和46.1%,对照组依次为77.8%、44.4%和33.3%.结论 Ⅲa期非小细胞肺癌手术并术中置药术后胸腔灌注,辅以早期联合静脉化疗,可提高患者生活质量及生存率. Abstract： Objective To investigate an effective approach for stage Ⅲ a non- small cell lung cancer. Methods The stage Ⅲ a non-small cell lung cancer were divided into observation group (26 cases) and control group(27 cases). The observation were set THP 10 mg, CTX 200 mg, CBP 50 mg on lymph during the operation and were given THP 50 mg, CTX 600 mg, CBP 300 mg, IFN-α thoracic infusion chemotherapy after surgery. All of two groups(53 cases)received alternate intravenous chemotherapy of PTX + THP + CBP/DDP or GEM + CBP/DDP or PTX + L-OHP or NVB + L-OHP regimens in 3weeks after surgery and repeat every 4 weeks. The patients had 4 to 6 therapeutic course. The follow-up survery was compared after the treatment between the two groups. Results 1,2,3 years survival rates were 88.4% ,76. 9% and 46.1% in the observation group as compared with 77.8% ,44.4% and 25.9% in the control grouup respectively. Conclusions Operation set chemicals in surgery and given thoracic infusion chemotherapy after surgery and combined chemotherapy is an effective way for treatment of stage Ⅲ a non -small cell lung cancer. It can improve the life quality and survival rates of the patients.     

非小细胞肺癌组织中Survivin与Fas/FasL的表达及其临床意义

目的 检测非小细胞肺癌(NSCLC)组织中Survivin、Fas/FasL的表达.方法 采用免疫组织化学法检测16例正常肺组织、48例肺鳞癌组织、38例肺腺癌组织中Survivin、Fas/FasL的表达.结果 正常肺组织中Survivin的阳性表达率为0,而在肺鳞癌和肺腺癌组织中Survivin的阳性表达率分别为79.17%和70.05%,与正常肺组织相比二者差异均有统计学意义(P<0.01);正常肺组织中Fas的阳性表达率为81.25%,与肺鳞癌(41.67%)和肺腺癌(43.37%)相比,差异均有统计学意义(P<0.05);Fas的表达与性别、组织学类型、TNM分期及有无淋巴结转移均无相关性.正常肺组织中FasL的阳性表达率为18.75%,低于肺鳞癌(72.92%)和肺腺癌(68.42%),P<0.05.NSCLC组织中FasL的表达与性别及组织学类型无关,与TNM分期和淋巴结转移有关.结论 Survivin基因异常表达而引起的细胞凋亡抑制在NSCLC的发生中起一定的作用,其过度表达提示NSCLC预后不良;NSCLC Fas低表达在其发病中可能有一定的作用;Fas/FasL可能参与了NSCLC凋亡的调节,在NSCLC逃逸机体免疫监控方面起重要作用,因此检测FasL的表达可能为临床判断肺癌淋巴结转移提供依据. Abstract： Objective To detect the expression of Survivin, Fas/FasL in non-small cell lung cancer(NSCLC) tissue. Methods We used immunohistochemistry to detect the expression of Survivin, Fas/FasL in 16 cases of normal lung, 48 cases of squamous carcinoma of lung tissue, 38 cases of adenocarcinoma of lung tissue. Results The positive rate of Survivin in normal lung tissue, squamous carcinoma and adenocarcinoma of lung were 0, 79.17% and 70.05% respectively, and there was significant difference between the normal and the two NSCLC groups(P<0.01). The positive rate of Fas in normal lung tissue(81.25%) was significantly higher than that in squamous carcinoma of lung(41.76%) and adenocarcinoma of lung(43.37%),P<0.05. The positive of FasL in normal lung tissue(18.75%) was significantly lower than that in SqCa(72.92%) and adenocarcinoma of lung(68.42%)(P<0.05). No relation was found between the expression of FasL and clinicopathologic factors including sex, histoclassification. And there was a relation between lymphnode metastasis and TNM stage of NSCLC. Conclusions Apoptosis inhibitition caused by abnormal survivin expression may participate in the onset and progression of NSCLC. The over-expression survivin suggests the bad prognosis in NSCLC. The expression of Fas protein in NSCLC was low, and might play a role in carcinogenesis of lung carcinoma. The expression of Fas/FasL in NSCLC may play an important role in the escape of tumor cells from immune function. The expression of FasL might be used as an objective indicator of metastasis potency of local lymph node.     

基质金属蛋白酶及其组织抑制因子在肺癌中的表达及临床意义

目的 探讨基质金属蛋白酶MMP-2及其组织抑制因子-2与肺癌侵袭和转移的关系.方法 选取肺癌住院手术患者共45例,其中男30例,女15例,鳞癌、腺癌、小细胞肺癌各15例.术后临床分期Ⅰ期15例,Ⅱ期20例,Ⅲ期10例.取上述肺癌患者切除标本距离肿瘤组织3cm以外的正常组织15例为组织对照组,采用免疫组化S-P法分别对肺癌患者组织中的MMP-2及TIMP-2进行测定,采用X2检验进行统计学分析.结果 肺癌组织中,MMP-2、TIMP-2组织表达明显高于对照组(P＜0.01,P＜0.05),在Ⅰ、Ⅱ、Ⅲ期中,MMP-2在组织中的表达随分期的升高而增加(P＜0.05),但TIMP-2的表达则相反,随肿瘤分期增加而降低(P＜0.05).结论 基质金属蛋白酶-2在肺癌侵袭和转移中具有重要的作用,可以用来评价肿瘤的恶性程度和估计预后.