黄热病毒与甲型流感病毒及其分型集合检测芯片的初步研制

目的：研制黄热病毒与流感病毒及其分型集合检测芯片。方法：设计并合成60mer寡核苷酸（Oligo）探针用于制备检测芯片。提取黄热病、甲型流感病毒核酸,以限制性显示技术进行扩增标记,完成杂交后对芯片进行扫描和数据分析。结果：Oligo探针与相应的荧光标记样本杂交后,大部分能检测出阳性荧光信号,而空白对照和阴性对照均为阴性信号。结论：寡核苷酸芯片技术可应用于多种病毒的检测及分型,从而为多种病毒感染的早期鉴别诊断提供科学依据。     

静脉药瘾者肝炎病毒流行病学及TH细胞功能的研究

[目的]探讨静脉药瘾者血源性肝炎病毒（HBV,HCV,HDV,HGV）的流行病学情况,以及TH细胞因子变化与病毒感染的关系。[方法]收集406例静脉药瘾者和102例健康体检者静脉血,采用酶联免疫吸附法和荧光定量PCR检测HBV、HCV、HDV和HGV的感染状况,病毒感染阳性者同时进行血清丙胺酸转氨酶（ALT）检测。并对同一标本用酶联免疫吸附法、放射免疫法检测外周血单个核细胞诱生的IFN-γ和IL-4,血清中IL-2和IL-4水平。[结果]静脉药瘾者HBV、HCV、HDV和HGV的感染率分别是36.45%、69.7%、2.22%和1.97%,HBV和HCV的重叠感染人数为113人（27.83%）。对照组仅发现存在HBV感染（17.65%）,其余病毒感染指标均为阴性,两者差异有统计学意义（P﹤0.05）。静脉药瘾者单个核细胞诱生的IFN-γ、IL-4以及血清IL-2比对照组减低;血清IL-4增高,差异有统计学意义（P﹤0.01）,ALT异常者其血清IL-4明显增高。静脉药瘾者发生HBV和HCV感染时,单个核细胞诱生的IFN-γ进一步降低。[结论]静脉药瘾者血源性肝炎病毒主要是HBV和HCV,其TH细胞因子分泌减少,TH1和TH2细胞平衡紊乱,而肝炎病毒感染可进一步降低TH1产生IFN-γ水平,异常ALT是导致血清IL-4升高的因素。     

延边农村地区朝鲜族HBV，HCV，HDV感染状况调查

为摸清延边农村地区朝鲜族人群乙,丙、丁型肝炎病毒（HBV,HCV,HDV）感染状况,用ELISA法对585名朝鲜族成年人进行了HBV,HCV和HDV感染标志物的检测,HBV,HCV及重经为2．56％,HZBV与HCV的感染相关无显著性,42例HBsAg阳性者中HDV感染率4．76％,HBV感染模式有15种,其中以单项抗－HBs阳性多见,人群中占31．97％,表明人群中HBV,HCV和HDV感染的流行情况不同。     

延边农村地区朝鲜族HBV、HCV、HDV感染状况调查

为摸清延边农村地区朝鲜族人群乙、丙、丁型肝炎病毒(HBV、HCV、HDV)感染状况,用ELISA法对585名朝鲜族成年人进行了HBV、HCV和HDV感染标志物的检测.HBV、HCV标化感染率与HBsAg标化阳性率分别为50.91%、4.85%和8.57%,年龄组间差异无显著性.HBV、HCV双重感染率为2.56%,HBV与HCV的感染相关无显著性.42例HBsAg阳性者中HDV感染率4.76%.HBV感染模式有15种,其中以单项抗-HBs阳性多见,人群中占31.97%.表明人群中HBV、HCV和HDV感染的流行情况不同.     

多种病毒集合检测芯片的研制及其在临床血清标本检测中的初步应用

目的 研制黄热病、西尼罗热、登革热、基孔肯雅热、埃博拉出血热、马尔堡出血热、拉沙热等多种病毒性传染病集合检测基因芯片,并建立一种适用于直接检测核酸含量较低临床血清标本的新型芯片靶基因扩增标记方法.方法 设计并筛选出上述病毒70mer寡核苷酸特异探针各20条,打印于同一基因芯片上；以phi29 DNA聚合酶结合带标签序列的随机引物进行临床标本中病毒全基因组扩增,再以Cy3荧光染料标记的标签序列引物进行PCR随机扩增标记,标记产物用多种病毒芯片进行杂交检测.结果 检测1～4型登革热、基孔肯雅热病例临床血清标本,结果显示该方法准确、特异、敏感；检测西尼罗热、黄热病、埃博拉出血热、马尔堡出血热、拉沙热等病毒核酸的模拟血清标本,同样得到特异的阳性杂交信号,与预期结果一致.结论 本研究建立的多种病毒集合检测基因芯片及其靶基因扩增标记方法,可直接应用于血清标本中上述病毒核酸的检测.     

膜芯片技术诊断乙型和丙型肝炎病毒与基因分型

[目的]利用膜芯片技术建立乙型肝炎病毒(HBV)和丙型肝炎病毒(HCV)联合诊断和基因分型新方法。[方法]根据HBV的前C区、X区及HCV的5'端非编码区(5'UTR)基因序列分别设计引物与分型探针。用生物素标记的引物进行HBV-DNA和HCV-cDNA扩增,将活性氨基标记的分型探针依次固定在尼龙膜上,制成HBV和HCV联合诊断和基因分型膜芯片条。扩增产物与膜芯片条杂交后,经过氧化物酶与底物显色,判断有无HBV和HCV感染并同时进行基因分型。通过经荧光定量PCR和基因测序确定的HBV和HCV单项阳性血清各30份以及HBV和HCV混合血清5份验证该方法的有效性。[结果]膜芯片对HBV和HCV单独感染和混合感染的诊断与荧光定量PCR结果相同,基因分型结果与测序分型结果一致。[结论]利用膜芯片技术可实现HBV和HCV联合诊断和基因分型,并具有准确有效和简便经济等特点,适合用于临床诊断和流行病学调查。     

乙型肝炎病毒感染患者的丁型肝炎标志的检测

目的：了解乙型肝炎病毒（HBV）感染者中丁型肝炎病毒（HDV）感染情况，方法：对30例肝癌和40例肝硬化患者HBV复制标记和HDB感染标志的检测。结果：慢性肝病患者中HDV感染率为17．4％，肝癌患者HDV检出率低于肝硬化患者，在HBV复制阳性组中HDV检出率为3．33％，阴性组为27．5％，两者差异有显著性（P＜0．01）。结论：HDV感染抑制了HBV的复制，通过检测发现肝硬化病情加重的原因与HBV活跃复制及HDV重叠感染有关，而肝癌病情恶化与HBV复制活跃及HDV重叠感染关系不大。     

基因芯片的概念及应用（基础）

一、基本概念 基因芯片也叫DNA芯片、DNA微阵列、寡核苷酸阵列，是指采用原位合成或显微打印手段，将数以万计的DNA探针固化于支持物表面上，产生二维DNA探针阵列，然后与标记的样品进行杂交，通过检测杂交信号来实现对生物样品快速、并行、高效地检测或医学诊断，由于常用硅芯片作为固相支持物，且在制备过程运用了计算机芯片的制备技术，所以称之为基因芯片技术。     

丁型肝炎病毒母婴垂直传播的研究

为探讨丁型肝炎病毒母婴垂直传播，我们采用酶免疫测定法（EIA）检测长春地区50例乙型肝炎病毒标志物（HBV－M）阳性孕妇血清及其新生儿脐血清中丁型肝炎病毒标志物（HDV－M）。结果，孕妇血清HDV-M阳性8例，阳性率为16％（8／50），新生儿脐血清HDV－M阳性2例，阳性率为4％（2／50）。表明长春地区确有丁型肝炎病毒（HDV）感染，且HDV可通过胎盘垂直传播给胎儿。     

杭州地区妊娠晚期孕妇病毒性肝炎感染的调查研究

目的:调查杭州市2958例妊娠晚期孕妇乙型肝炎病毒(HBV)、丙型肝炎病毒(HCV)、丁型肝炎病毒(HDV)、戊型肝炎病毒(HEV)的感染状况。方法:对杭州市2958例妊娠晚期孕妇的血清乙型肝炎标志物、HCV抗体、HDV抗体、HEV抗体的检测结果进行统计分析。结果:2958例妊娠晚期孕妇中乙型肝炎总感染率40.16%,HCV抗体阳性率0.40%,HDV抗体阳性率0.20%,HEV抗体阳性率2.60%。结论:杭州地区妊娠晚期孕妇病毒性肝炎的感染以乙型肝炎位居第一、戊型肝炎次之,丙型肝炎及丁型肝炎感染率较低。应加强育龄女性易感人群乙肝疫苗的预防接种,开展和实施有效的预防措施来防止病毒性肝炎的感染,提高新生人口的素质。     

缬沙坦/氨氯地平和依贝沙坦/双氢克尿噻联合治疗老龄高血压疗效的研究

Objective To compare the clinical effect of valsartan/amlodipine combination or irbesartan/hydrochlorothiazide(HCTZ)combination in very elderly hypertensives.Methods After a 4-week placebo period,94 hypertensives,aged 75-89 years were random given valsartan 160 mg/amlodipine 5 mg or irbesartan 300 mg/HCTZ 12.5 mg for 24 weeks according to a rospective study.After 4 weeks,amlodipine or HCTZ was doubled in non-responders.Patients were checked every 4 weeks.At each visit,sitting,lying and standing blood pressure(BP),systolic BP(SBP)and diastolic BP(DBP)were measured. At the end of placebo period and treatment period,electrolytes and uric acid were evaluated.Results Blood pressure was significantly decreased in both treatment groups,however,there was no statistical significance between two groups.BP changes from lying to standing position were significantly greater in the irbosartan/HCTZ group(-17.2/-9.1 mmHg)than that in the valsartan/amlodipine group(-10.1/-1.9 mmHg,t=2.14,P＜0.05 for SBP and t=3.11,P＜0.01 for DBP vs.irbesartan/HCTZ).Potassium significantly decreased and uric acid significantly increased(-0.4 mmol/L,t = 2.33,P＜ 0.05 and+29.7μ mol/L,t =2.54,P＜0.05 vs.baseline,respectively)only in the irbesartan/HCTZ group.Conclusions Both combinations had similarly effective in reducing clinical BP in very elderly hypertensives.However,valsartan/amlodipine offered some advantage and less pronounced BP orthostatic changes and absence of metabolic adverse effects.     

乙酰半胱氨酸对肥胖哮喘小鼠气道炎症和重建的作用

目的探讨乙酰半胱氨酸对哮喘气道炎症和重建的影响。方法60只雌性C57／6J小鼠按随机数字表法分为哮喘组（A组）,肥胖哮喘组（B组）,治疗组（C组）,对照组（D组）,每组15只。经腹腔注射与雾化吸入卵蛋白（OVA）制作慢性哮喘模型,高脂饮食制造肥胖模型。计数支气管肺泡灌洗液（BALF）中细胞总数及分类,ELISA法测定肺组织匀浆中IL-6和8-异前列腺素F2α（8-iso-PGF2α）水平,肺组织切片观察各组小鼠病理变化,并测定气管壁面积（WAt）、气管平滑肌面积（WAm）、管腔基底膜周长（Pbm）。结果,A组、B组小鼠BALF中白细胞总数及嗜酸性粒细胞比例、肺组织IL-6水以及病理切片中气管壁厚度（WAt／Pbm）、气管平滑肌厚度（WAm／Pbm）均较D组明显增加,且B组IL-6和WAt／Pbm较A组进一步增加（P〈0．05）。C组小鼠BALF中白细胞总数、IL-6和WAt／Pbm均较B组明显下降（P〈0．05）。四组小鼠肺组织匀浆8-iso—PGF2α水平按D组、C组、A组、B组依次升高,各组差异有统计学意义（F=101．8,P〈0．01）。Pearson相关分析表明,8-iso—PGF2α水平与肺组织IL-6水平和WAt／Pbm和呈正相关（r=0．817、0．737,P〈0．01）。结论乙酰半胱氨酸能够通过抑制氧化应激反应,抑制哮喘小鼠的气道炎症和气道重建。     

脂多糖对人皮肤成纤维细胞胶原代谢的影响

目的探讨脂多糖（1ipopolysaccharide,LPS）对人皮肤成纤维细胞胶原代谢的影响,以了解LPS在增生性瘢痕形成中的生物学作用。方法取正常皮肤行成纤维细胞培养后,分为1个对照组及6个实验组。实验组分别与终浓度为0．005、0．010、0．050、0．100、0．500和1．000μg／ml大肠杆菌LPS（Ecoli055：B5）培养,对照组DMEM培养。用逆转录-聚合酶链反应（RT-PCR）法测定成纤维细胞Ⅰ、Ⅲ型前胶原mRNA及胶原酶mRNA的表达,并以同一个体相同代数的瘢痕组织成纤维细胞做对照。结果与对照组比较,LPS刺激浓度在0．005-0．1μg／ml时,促进正常皮肤成纤维细胞Ⅰ、Ⅲ型前胶原mRNA表达（0．323±0．041,0．303±0．063,0．391±0．071,0．344±0．086,0．488±0．059,0．401±0．087,0．616±0．107,0．434±0．084,0．823±0．092,0．542±0．082）,抑制胶原酶mR-NA表达（0．598±0．068,0．556±0．049,0．441±0．043,0．372±0．083,0．260±0．027）,且呈一定的剂量依赖性;当LPS刺激浓度为0．5μ／ml,上述作用下降（0．451±0．063,0．374±0．072,0．360±0．062）;而当LPS刺激浓度到达1．0μg/ml时,抑制正常皮肤成纤维细胞Ⅰ、Ⅲ型前胶原mRNA表达（0．162±0．025,0．171±0．061）,促进胶原酶mRNA表达（0．444±0．114）。LPS刺激浓度在0．1μg／ml时,成纤维细胞（0．823±0．092,0．542±0．082,0．260±0．027）Ⅰ、Ⅲ型前胶原mRNA和胶原酶mRNA表达与同一个体增生性瘢痕组织成纤维细胞（0．829±0．049,0．569±0．038,0．277±0．059）近似。结论LPS对人皮肤成纤维细胞Ⅰ、Ⅲ型前胶原mRNA和胶原酶mRNA的表达,其直接调节可能是参与增生性瘢痕形成的重要机制。     

异丙酚对大鼠肺缺血/再灌注损伤的保护作用

Objective To study the protective effects of propofol against ischemia/reperfusion injury in rat lung. Methods Rat model of pulmonary ischemia/reperfusion injury was used in this study. Rats were randomly divided into three groups, including sham opera-tion group (group A), iachemia/reperfusion group (group B) and propofol group (group C), 15 rats in each group. The concentration of tumor necrosis factor -α and interleukin-6 in bronchoalveolar lavage fluid (BALF) were measured by enzyme linked immunosorbent assay (ELISA). Then blood gas analysis, lung wet/dry (W/D) weight ratio were detected in each group. Results Propofol could significantly improve PaO2, reduce the W/D value and the contents of TNF-α and IL-6 in BALF. Conclusion Propofol effectively suppressed the pro-duction and release of inflammatory cytokine, therefore it can protect the lung from isehemia/reperfusion injury.     

降钙素对大白兔糖尿病骨质疏松模型假体骨密度及生物力学的影响

Objective To investigate the influence of calcitonin on bone mineral density and biomechanics around the artificial pros-thesis in ovariectomized diabetic rabbit model. Methods Fourteen femina New Zealand white rabbits at the age of 5 months old were select-ed, which weight 2.24 -2.65kg, averaging 2.26kg. First, the model of rabbit with diabetic osteoporosis was successfully established by the compound method of ovariectomy plus streptozotocin. Osteotomy in the middle part of femur was performed in both groups, fixation of artifi-cial prosthesis was done with 3.0 kirschner wire. After that, Rabbit models with diabetic osteoporosis were randomly divided into experimen-tal group and control group. Rabbits in the experimental group were treated with calcitonin 6U intramuscular injection once every other day. In control group, intramuscular injection of normal saline solution 1.5ml once every three days. Rabbit models of two groups were sacrificed in the 24th week. The BMD of the region of interest (ROI) around the prosthesis were detected before experiment and 8, 16 and 24 weeks after injection. After rabbits were killed, experimental femurs in both groups were complete removal and soft tissues were rejected. Determi-nation of the pull-out and torsion bone biomechanics experiments of prosthesis was done in both groups respectively. Results The BMD of ROI in the experimental group before operation was (0.1863±0.004)g/cm2 and (0.1753±0.005)g/cm2 in 24 weeks after operation, in control group before operation was (0.1865±0.002)g/cm2 and (0.1638±0.005)g/cm2 in 24 weeks after operation. There were significant difference between the two groups(P < 0.05). Biomechanical show that the pull-out strength in the experimental group was (312.68±8.73 )N/cm2 and (205.43±12.45 ) N/cm2 in control group. There were significant difference between the two groups(P < 0.05). The tor-sion strength in experimental group was (80.47±2.51) N/cm2 and (38.52±0.64) N/cm2 in control group. There were significant differ-ence between the two groups(P < 0.05). Conclusion Salmon calcitonin can reduce the bone turnover rate around prosthesis and decrease bone absorption in the rabbit of diabetic osteoporosis models, accelerate the bone formation around prosthesis, and increase the BMD. It can ameliorate the quality of bone around prosthesis, improve its biomechanics property, and increase the holding power between prosthesis and body mass. It is of clinical significance for the prevention and treatment of aseptic loosening artificial prosthesis.     

蝶鞍结构与垂体瘤生长方式关系的影像学研究

目的 从影像学角度探讨蝶鞍相关解剖对垂体腺瘤生长方式的潜在影响.方法 按照常用蝶窦分类方法,共收集83例蝶窦气化类型为全鞍型和20例鞍前型正常头颅的影像学资料;同时收集45例影像学提示为侵入蝶窦生长的垂体腺瘤临床资料进行分析.结果 （1）正常鞍底形态与蝶窦气化程度的关系：全鞍型蝶窦更易导致凹陷型鞍底（98.8%）.（2）正常鞍底形态与垂体上缘形态的关系：凹陷型垂体上缘更易伴随凹陷型鞍底（93.8%）.（3）根据正常头颅正中矢状位鞍窝骨性上口前后径与鞍窝前后最大径之间的关系,对蝶鞍形态提出初步分型：囊袋型、炒锅型、直筒型及筛型.（4）所有向蝶窦生长的垂体腺瘤患者蝶窦均为全鞍型.结论 鞍底形态、蝶窦气化程度及蝶鞍形态等综合因素能够解释垂体腺瘤向鞍底蝶窦生长的现象;结合文献,蝶鞍的相关形态学基础应该是影响垂体腺瘤生长方式的主要因素.     

强直性脊柱炎合并骨质疏松的临床研究

Objective To investigate the incidence of osteoporosis (OP) in patients with ankylosing spondylitis and the relationship between OP and the clinical data. Methods Serum levels of bone alkaline phosphatase (BALP) and tartrate resistant acid phosphatase 5b (TRACP5b) were detected by enzyme linked immunosorbent assay ( ELISA ) in 60 cases with ankylosing spondylitis, and it was compared with normal controls. Bone mineral density (BMD) was measured through dual-energy X-ray absorptiometry ( DXA), including lumbar ( L2 - L4), bilateral femoral neck and greater trochanter. Some clinical data was collected and analyzed at the same time. Results The incidence of OP in AS patients was 35%, and the incidence of OP in the femoral proximal end was higher than that in lumbar. Compared with normal controls[ ( 1.06 ±0. 18 )U/L ], the levels of serum TRACP5b in AS[ (1.31 ± 0. 82 )U/L] patients was significantly higher ( P <0. 05 ). The levels of serum BLAP in OP combined AS group[ ( 21.65 ± 5.41 ) U/L]were significantly lower than non-OP group[ (32. 37 ± 16. 5 ) U/L] ( P <0. 05 ). The disease duration was negatively correlated with the BMD of femoral neck ( P < 0. 01 ). Conclusions There was higher incidence of OP in AS patients, which were related with the abnormality of bone metabolism and the disease duration.Multiple factors participated in the regulation of bone metabolism of AS.     

关节腔内注射洛伐他汀预防骨关节炎软骨退变

目的 研究洛伐他汀关节腔内注射对骨关节炎(OA)模型关节软骨退变及对基质金属蛋白酶(matrix metalloprotein-ase-1,3)mRNA表达的影响.方法 30只6个月龄新西兰大白兔行右膝关节前交叉韧带切断术.手术后将动物随机分为实验组和对照组,实验组术后立即给予关节腔内注射0.5 mg/ml洛伐他汀0.2 ml/kg,每周1次,连续6周;对照组则关节腔内注射等容量的生理盐水.术后6周处死动物.在解剖镜下观察股骨内髁关节软骨的大体形态学改变并评分.用反转录-聚合酶链式反应检测软骨及滑膜中MMP-1,3 mRNA的表达.结果 解剖镜下实验组软骨退变程度较对照组明显减轻;实验组关节液中IL-1水平较对照组明显降低;实验组滑膜中MMP-1,3 mRNA的表达水平显著低于对照组(P<0.05),而软骨中的MMP-1,3 mRNA的表达较对照组差异无统计学意义(P>0.05).结论 在OA早期关节腔内注射L洛伐他汀能够明显降低MMP-1,3 mRNA的表达,减轻骨关节炎软骨的退变.     

利尿剂在三聚氰胺及三聚氰酸致大鼠急性肾损伤中的治疗作用

Objective To investigate the effect of diuretic (furosemide) therapy on kidney injury induced by melamine and cyanuric acid in rats. Methods 36 male Spragne Dawley rats were random disided into 3 groups. Group A was treated with 2mL of water daily, group B was treated with melamine and cyanuric acid ( each 100 mg/kg) daily for 4 days and then 2ml of water daily, group C was treated with the same as group B at the first 4 days and then treatment with furosemide (20mg/kg) daily. Samples of blood and 24h urine were collected to detective biochemical indexes, and kidney sections were performed on days 4 and 11 ( each end point, n = 6). The kidneys were observed with histopathology and renal crystal deposition scores were determined. Results On the 4th day, group B and group C were resulted in acute kidney injury such as oliguria [ ( 3. 39 ± 1.02 ) ml, ( 3. 20 ± 0. 86 ) ml ] and high serum creatinine [ ( 153.54 ±27. 08)μmol/L, (160. 11 ± 19. 55)μmol/L] and renal melamine cyanurate crystal were found in the renal tissues. On the 11th day, the renal crystal deposition score in the rats was reduced by 9. 52% ( P >0. 05). Compared with those of the 4th day in group B, it reduced by 63.63%( P <0.05) in group C. Urine volume were increased significantly compared with those of the 4th day( P < 0. 05 ) in group C [ from (3.20±0. 86)ml to (25.96 ±5.97)ml] and group B [ from(3. 39 ± 1.02)ml to (8. 57 ± 1.66)ml] , and Urine volume in group C was increased significantly more than that in group B ( P < 0. 05 ). The serum creatinine was obviously reduced as compared with those of the 4th day in group B and C( P <0.05), from[ (153. 54±27.08) μmol/L] to [ ( 106. 10 ±5.53) μmol/L] in group B and from [ ( 160. 11 ± 19. 55) μmol/L] to [ (67. 17 ± 12. 80 ) μmol/L] in group C, but the serum creatinine in group B was still higher than that in group A and C ( P < 0. 05). Conclusions Furosemide can attenuate the damage of acute kidney injury induced by melamine and cyanuric acid.     

脓毒症血清对大鼠小肠微血管内皮细胞ICAM-1的影响及肾上腺髓质素的干预作用

Objective To observe the levels of intercellar adhesion molecule-1(ICAM-1)of rat intestinal mucosa microvascular endothelial cells in sepsis rat,and to investigate the possible protective effect of adrenomedullin on the expression of intercellar adhesion molecule-1(ICAM-1)of rat intestinal mucosa microvascular endothelial cells,and to confirm whether adrenomedullin can decrease released inflammatory factors to slow down or inhibit the occurrence and development of sepsis.Methods The rat intestinal mucosa microvascular endothelial cells were isolated and cultured in vitro,and it was divided into sham operated(sham operated serum),sepsis serum(with addition of sepsis serum)and ADM serum(with 0.lng/kg adrenomedullin 6h after sepsis treatment)groups.The soluble ICAM-1(sICAM-1)content in RIMMVECs culture supernatant was measured by ELISA at the 24th hour after serum stimulation.Results The ICAM-1 content in the supernatant of sepsis group [(0.33 ±0.04)ng/L] was obviously higher than that in sham operated [(0.15 ± 0.02)ng/L] and ADM [(0.17 ± 0.04)ng/L] group(P ＜ 0.05),but ADM group was no significantly different compared with sham operated group(P ＞ 0.05).Conclusions ADM could decrease the expression of ICAM-I in RIMMVECs,and the low level of ICAM-1 could suppress its downstream inflammation factors and inhibit the occurrence and development of sepsis.