涎腺正常与肿瘤组织中p63、CK5、CK19、CEA及SmA的表达

目的:探索涎腺正常导管、腺泡及其上皮性肿瘤的组织起源.方法:采用免疫组化(二步法)检测正常涎腺组织14 例、多形性腺瘤14 例、基底细胞腺瘤8 例、腺样囊性癌18 例、黏液表皮样癌9 例、低分化腺癌2 例及腺泡细胞癌、乳头状腺癌、恶性多形性腺瘤、肌上皮癌各1 例中p63、CK5、CK19、CEA及SmA的表达.结果:正常涎腺基底细胞与肌上皮细胞层表达CK5,其中部分细胞表达p63,导管部分基底细胞也呈CK19阳性.多形性腺瘤、基底细胞腺瘤、腺样囊性癌与黏液表皮样癌具p63、CK5及CK19高阳性率, SmA阳性率分别为85.71%、1.25%、61.11%、0.00%.CEA阳性细胞见于11.11%腺样囊性癌与55.56%黏液表皮样癌.结论:正常涎腺导管和腺泡的腺上皮及涎腺上皮性肿瘤可能来源于导管、腺泡基底层内呈p63和CK5阳性细胞中的干细胞.     

EphA2在涎腺肿瘤中的表达及其临床意义

目的检测EphA2在涎腺肿瘤中的表达，并分析其临床病理意义。方法免疫组化S-P法检测10例正常涎腺组织、30例多形性腺瘤、30例黏液表皮样癌（MEC）、30例腺样囊性癌（ACC）中EphA2的表达。分析EphA2表达与患者的年龄、性别、肿瘤部位、大小、TNM分期以及复发、淋巴结转移等临床病理因素的关系。比较EphA2在正常涎腺组织及几种常见的涎腺良、恶性肿瘤中的表达差异。结果EphA2在正常涎腺组织中主要表达于腺管上皮，而腺泡细胞几乎不表达。EphA2在正常涎腺组织、多形性腺瘤、涎腺癌（包括黏液表皮样癌和腺样囊性癌2组）中的表达依次增高，存在显著性差异（P〈0．05）。EphA2的表达与涎腺癌瘤体大小、TNM分期、复发和淋巴结转移有关（P〈0．05）。结论EphA2可能影响涎腺肿瘤发生、发展及其预后。     

涎腺肿瘤中Pax9基因的表达及其意义

目的:检测正常涎腺组织及涎腺肿瘤中转录因子Pax9的表达。方法:采用免疫组化SABC法,研究Pax9在正常涎腺组织、多形性腺瘤、腺淋巴瘤、基底细胞腺瘤、粘液表皮样癌和腺样囊性癌共48例中的表达情况。结果:除1例多形性腺瘤外,其余组织均表达Pax9。正常涎腺的腺泡细胞和导管内衬细胞、多形性腺瘤的上皮细胞、腺淋巴瘤的肿瘤上皮细胞和基底细胞腺瘤中Pax9弱阳性表达,差异没有显著性;而在增殖活跃的细胞如正常涎腺导管的基底细胞和恶性肿瘤细胞(粘液表皮样癌、腺样囊性癌)中表达增强,恶性肿瘤中Pax9的强阳性表达率明显增高,与正常和良性肿瘤相比具有显著差异(P<0.05)。结论:Pax9在涎腺肿瘤尤其是恶性肿瘤的发生过程中发挥重要的作用。     

2489例涎腺上皮性肿瘤临床病理分析

目的:了解涎腺上皮性肿瘤的临床病理特点。方法:对2489例涎腺上皮性肿瘤临床病理资料进行统计分析。结果:涎腺恶性上皮性肿瘤840例,腺样囊性癌、黏液表皮样癌、癌在多形性腺瘤中居其前3位;涎腺良性上皮性肿瘤1649例,多形性腺瘤、Warthin瘤、基底细胞腺瘤居其前3位。涎腺恶性、良性上皮性肿瘤男女之比为1.13∶1和0.99∶1;平均发病年龄47.86岁和44.86岁;腮腺和腭部为好发部位。结论:腺样囊性癌和多形性腺瘤是最常见的涎腺恶性、良性上皮性肿瘤。     

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涎腺上皮性肿瘤组织病理学结构具有器官特异性,典型的多形性腺瘤、Warthin瘤、黏液表皮样癌和腺样囊性癌等肿瘤病理诊断较容易。涎腺肿瘤有多种病理学类型,且     

涎腺疾病

腮腺肿瘤手术中冰冻活检的意义；腮腺多形性腺瘤113例临床分析；抑癌基因PTEN、p27及Ki-67在涎腺黏液表皮样癌中的表达；人涎腺腺样囊性癌神经侵袭动物模型的建立；内镜辅助诊断慢性阻塞性腮腺炎的临床应用；腮腺淋巴结炎继发化脓性腮腺炎的CT及声像图特点；微血管密度和涎腺腺样囊性癌远处转移及预后的关系；     

增殖细胞核抗原和Ki-67抗原在涎腺癌中的表达

目的:探讨细胞增殖核抗原和Ki-67抗原在不同类型涎腺癌中的表达及意义。方法:采用SABC和LSAB免疫组化法分别检测PCNA和Ki-67在17例粘液表皮样癌、12例腺样囊性癌、9例恶性多形性腺瘤中的表达和分布。结果:1)PCNA及Ki-67的增殖指数即PI值在粘液表皮样癌和腺样囊性癌的不同分级中表达不同,且有统计学意义(P<0.005)。2)恶性多形性腺瘤PCNA及Ki-67的PI值较高,与腺样囊性癌及粘液表皮样癌相比差异有统计学意义(P<0.001)。3)PCNA及Ki-67的表达水平与涎腺癌的生物学行为(复发和转移)明显相关。结论:涎腺癌中PCNA和Ki-67的表达水平与细胞分化有关,其PI值可作为粘液表皮样癌和腺样囊性癌的组织学分级的重要参数,也是诊断恶性多形性腺瘤的重要指标。PCNA和Ki-67的高表达预示着涎腺癌预后的不良。     

细胞外基质金属蛋白酶诱导因子表达与唾液腺肿瘤侵袭性的关系

目的:检测唾液腺肿瘤组织中细胞外基质金属蛋白酶诱导因子(EMMPRIN)的表达和定位,探讨其与唾液腺肿瘤侵袭性生物学行为的关系。方法:采用免疫组织化学方法,检测9例唾液腺正常组织、28例多形性腺瘤(PA)、25例黏液表皮样癌(MC)、33例腺样囊性癌(ACC)中EMMPRIN的表达和定位。采用SPSS10.0软件包进行多个样本率间的χ2检验或确切概率分析。结果:EMMPRIN在正常唾液腺组织、多形性腺瘤、黏液表皮样癌、腺样囊性癌的表达阳性率分别为11.11%、53.71%、84.00%和90.91%(P<0.05)。在正常唾液腺组织的表达主要见于导管上皮细胞的细胞膜;EMMPRIN蛋白在多形性腺瘤、黏液表皮样癌、腺样囊性癌的表达见于肿瘤细胞的胞膜或胞质。腺样囊性癌嗜神经现象组中,EMMPRIN表达的阳性率高于无嗜神经现象组,但差异无统计学意义。结论:EMMPRIN的表达与唾液腺肿瘤侵袭性生物学行为密切相关。     

癌基因c—erbB—2在涎腺肿瘤中的表达

作者利用抗c-erbB-2单克隆抗体,用免疫组化的方法,对50例涎腺肿瘤(腺癌,涎腺导管癌、粘液表皮样癌、腺样囊性癌、多形性腺瘤各10例)进行癌基因、c-erbB-2的检测。不同种类的涎腺肿瘤,其c-erbB-2的表达很不一致。腺癌及涎腺导管癌的阳性表达率较高,粘液表皮样癌及多形性腺瘤较低,腺样囊性癌为阴性。c-erbB-2的表达与肿瘤分化度有一定关联,涎腺肿瘤组织形态学的多样性,可能与c-erbB-2的表达不同有关。     

骨桥蛋白在涎腺良、恶性肿瘤中的表达及意义的研究

目的 探讨骨桥蛋白(OPN)在涎腺良、恶性肿瘤中的表达及意义。方法 采用免疫组化SP法检测OPN在68例涎腺肿瘤(多形性腺瘤20例、腺样囊性癌24例、Warthin瘤24例)和20例瘤旁正常涎腺组织中的表达。结果 OPN在多形性腺瘤和Warthin瘤中均较正常涎腺组织增高(P<0.05);在腺样囊性癌中较正常涎腺组织增高,但两组间比较差异无显著性(P>0.05)。结论 OPN在正常涎腺组织和腺样囊性癌中有弱表达,在多形性腺瘤和Warthin瘤中表达增高。     

涎腺肿瘤C-erbB-2癌基因mRNA表达的研究

目的 探讨Ｃ－ｅｒｂＢ２－ｍＲＮＡ表达水平同涎腺肿瘤组织类型、发生和生物学性质的关系。方法 采用ｄｏｔ ｂｌｏｔ杂交,以＾３２Ｐ标记的寡核苷酸探针,以正常涎腺为对照,对涎腺肿瘤Ｃ－ｅｒｂＢ－２ｍＲＮＡ表达进行研究。结果 正常涎腺和涎腺肿瘤中惺峭同程度Ｃ－ｅｒｂＢ－２ｍＲＮＡ的表达,以正常涎腺组织的表达水平为基准,在腺淋巴瘤、基底细胞腺瘤呈现低表达、无表达或正常涎腺组织表达水平相似,多形性腺瘤、粘液     

Expression of PLUNC family members in benign and malignant salivary gland tumours

Objectives: The aim of this study was to determine the expression of PLUNC proteins in benign and malignant salivary gland tumours and thus their potential use as diagnostic and / or prognostic tools. Materials and methods: A tissue microarray was assembled from 64 salivary gland tumours including adenoid cystic carcinoma, carcinoma ex‐pleomorphic adenoma, mucoepidermoid carcinoma, polymorphous low‐grade adenocarcinoma, pleomorphic adenoma, acinic cell carcinoma, myoepithelial carcinoma and papillary cystadenocarcinoma. Clinicopathological data were collected retrospectively and immunohistochemical analysis of three PLUNC proteins (SPLUNC1, SPLUNC2 and LPLUNC1) was performed. Immunoreactivity was assessed as positive or negative. Results: PLUNC expression was only found in mucoepidermoid carcinomas and papillary cystadenocarcinoma; all other tumours studied were negative. Mucin plugs, mucous and intermediate cells of mucoepidermoid carcinomas were positive for LPLUNC1 and SPLUNC2, but areas composed of epidermoid and clear cells were negative for all PLUNCs. Papillary cystadenocarcinoma was positive for all PLUNCs. No correlation was found with tumour grade or outcome. Conclusions: Intense expression of two PLUNC proteins in mucous cells and mucin plugs of mucoepidermoid carcinoma and papillary cystadenocarcinoma indicate that they could be used as additional diagnostic tools in some equivocal cases, but further studies are needed to understand the biological processes involved in PLUNC expression.     

唾液腺肿瘤中血小板衍生内皮细胞生长因子的表达和意义

目的:检测唾液腺肿瘤组织中血小板衍生内皮细胞生长因子(platelet derived endothelial cell growth factor,PD-ECGF)的表达及与微血管密度(microvessel density,MVD)值的相关性,探讨其在唾液腺常见肿瘤中的表达和意义。方法:用SP免疫组织化学法检测9例正常唾液腺组织、28例多形性腺瘤、25例黏液表皮样癌、33例腺样囊性癌组织中PD-ECGF的表达和MVD值。结果:PD-ECGF在正常唾液腺组织、多形性腺瘤、黏液表皮样癌、腺样囊性癌的表达阳性率分别为0、35.71%、80.00%、78.79%(P<0.05)。PD-ECGF主要表达于唾液腺肿瘤细胞的胞质中。PD-ECGF表达阳性的唾液腺肿瘤组织中MVD值高于PD-ECGF表达阴性的唾液腺肿瘤组织(P<0.05)。结论:PD-ECGF可能通过促进唾液腺肿瘤血管形成而在唾液腺肿瘤的生长和侵袭中发挥作用,可作为反映唾液腺肿瘤良、恶性的参考指标。     

PCNA, Ki-67 and p53 expressions in submandibular salivary gland tumours

Salivary gland tumours are uncommon with a broad heterogeneity. The most common benign tumour is the pleomorphic adenoma, whereas mucoepidermoid carcinoma and adenoid cystic carcinoma predominate among the malignancies. Most salivary gland tumours occur in the parotid, and consequently clinical and biological data are normally derived from this site. This work describes the expressions of PCNA, Ki-67 and p53 in 15 pleomorphic adenomas, 15 mucoepidermoid carcinomas and 15 adenoid cystic carcinomas of the submandibular gland. Our results showed that all pleomorphic adenomas were negative for p53 and Ki-67 with 66.6% being positive for PCNA. Conversely, p53 was positive in 53% of the mucoepidermoid carcinomas and in 20% of the adenoid cystic carcinomas. Ki-67 was expressed in 47.7% of the mucoepidermoid carcinomas and 40% of the adenoid cystic carcinomas. All malignant tumours were positive for PCNA. These results indicate that the proliferative rate analysed with PCNA and Ki-67 and the expression of p53 in pleomorphic adenoma and adenoid cystic carcinoma of the submandibular gland were similar to those described in the parotid and minor salivary glands. However, mucoepidermoid carcinomas showed higher expression of these markers than those of other salivary glands. This work is the first describing the expression of these immunohistochemical markers exclusively in submandibular salivary gland tumours.     

环氧化酶2、X染色体连锁凋亡抑制蛋白在唾液腺恶性肿瘤中的表达及意义

目的: 检测环氧化酶2(Cox-2)与X染色体连锁凋亡抑制蛋白(XIAP)在唾液腺腺样囊性癌(ACC)与黏液表皮样癌(MEC)中的表达,探讨两者在唾液腺恶性肿瘤发生、发展中的作用及相关性。方法:采用免疫组织化学SP法检测95例唾液腺恶性肿瘤(包括50例ACC、45例MEC)、10例多形性腺瘤及10例正常唾液腺组织中Cox-2和XIAP的表达情况,使用SPSS17.0软件包对数据进行统计学处理。结果:Cox-2、XIAP在唾液腺恶性肿瘤中高表达,在多形性腺瘤和正常唾液腺组织中不表达或低表达。Cox-2、XIAP的表达与唾液腺恶性肿瘤组织临床分期和远处转移呈正相关(P<0.05),而与肿瘤部位无相关性(P>0.05)。结论:Cox-2和XIAP在唾液腺恶性肿瘤中高表达,可能在唾液腺恶性肿瘤发生、发展过程中具有重要作用。     

Intraoral minor salivary gland tumors: a clinicopathological study of 82 cases

We present a retrospective study of 82 patients with intraoral minor salivary gland tumors which were diagnosed from 1979 to 2003 in Gifu University Hospital. The histological diagnoses were reevaluated according to the 1991 WHO classification. A total of 82 tumors, consisting of 55 benign and 27 malignant tumors, were found in 28 male and 54 female Japanese patients; the male-to-female ratio was 1:1.9. The mean age of the patients was 51.4+/-18.1 years. The tumors affected the palate (n = 64), the buccal region (n = 10), the upper lip (n = 6), the floor of the mouth (n = 1), and the retromolar region (n = 1). Histologically, the tumors were classified as pleomorphic adenoma (n = 54), papillary cystadenoma (n = 1), adenoid cystic carcinoma (n = 10), mucoepidermoid carcinoma (n = 8), acinic cell carcinoma (n = 3), adenocarcinoma (n = 2), basal cell adenocarcinoma (n = 1), papillary cystadenocarcinoma (n = 1), and carcinoma in pleomorphic adenoma (n = 2). From the results of the present study and review of the literature, it is suggested that the minor salivary gland tumors in Japan may be characterized by a higher incidence of benign tumors, especially of pleomorphic adenoma; a more marked tendency for female predominance; a higher incidence of palatal involvement; and a rarer occurrence of polymorphous low grade adenocarcinoma, in comparison with those reported in the literature from outside of Japan.     

The nucleolar organizer regions associated protein (Ag-NORs) in salivary gland tumors

A silver colloid technique used to identify nucleolar organizer regions associated protein (Ag-NORs) has been applied to 20 salivary gland tumors. The method was readily applicable to the preparations of paraffin-embedded sections and the Ag-NORs were enumerated with ease. A significant difference was found between the numbers of Ag-NORs in the nuclei of malignant salivary gland tumors, such as adenoid cystic carcinoma, mucoepidermoid tumor and adenocarcinoma (with a mean of from 2.05 to 2.78 per nucleus) and those of benign salivary gland, such as pleomorphic adenoma, adenolymphoma (Wartin tumor) and clear cell adenoma (with a mean of from 1.47 to 1.72 per nucleus). It is proposed that the Ag-NORs technique, which is rapid, simple, and inexpensive, may be useful in the differential diagnosis of malignant and benign salivary gland tumors.