Some molecular mechanisms of the antioxidative action of dalargin on the liver in experimental cholestasis

Clinical Biochemical Laboratory and Department of Anesthesiology and Resuscitation, A. V. Vishnevskii Institute of Surgery, Academy of Medical Sciences of the USSR, Moscow (Presented by Academician of the Academy of Medical Sciences of the USSR V. D. Fedorov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 113, No. 1, pp. 38–40, January, 1992.     

姜黄素预处理减轻急性心肌缺血大鼠心肌细胞凋亡

目的探讨姜黄素预处理减轻急性心肌缺血大鼠心肌细胞凋亡的机制。方法大鼠随机分为假手术组、心肌缺血2 h组及姜黄素预处理组,采用冠状动脉结扎的方法建立大鼠心肌缺血模型。用TUNEL法观察各组大鼠的心肌细胞凋亡情况;采用RT-PCR和Western blot检测各组大鼠心肌细胞凋亡相关因子mRNA和蛋白表达水平,并探讨它们之间的关系。结果与心肌缺血组相比,姜黄素预处理组BCL-2/β-actin/mRNA和蛋白表达相对值水平明显升高(P<0.05),心肌细胞凋亡数与BCL-2/β-actin蛋白表达相对值之间成明显的负相关(P<0.05);姜黄素预处理组caspase-3/β-actin mRNA和蛋白的表达相对值降低(P<0.05),心肌细胞凋亡数与caspase-3/β-actin mRNA和蛋白表达相对值之间成明显的正相关(P<0.05)。结论姜黄素对心肌缺血大鼠模型心肌梗死有明显保护作用,其作用机制可能与调节心肌细胞凋亡相关因子作用有关。     

Morphological and functional changes induced by dalargin in the pancreas in the normal state and in experimental pancreatitis

Central Research Laboratory, Andizhan Medical Institute. Department of Pathomorphology, N. V. Sklifosovskii Moscow Emergency Aid Research Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR D. S. Sarkisov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 105, No. 4, pp. 445–447, April, 1988.     

Fine Electrophysiological Analysis of Impulse Activity of Various Neuron Types in Nodose Ganglion during Intraatrial Injection of Dalargin

Experiments on narcotized cats showed that intraatrial injection of dalargin in doses crossing and not crossing the blood-brain barrier modifies impulse activity of nodose ganglion neurons.__________Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 139, No. 3, pp. 244–246, March, 2005     

Clofibrate and dalargin increase luminol-dependent chemiluminescence of mouse blood

The effects of hypolipidemic drug clofibrate and polypeptide dalargin on activity of the neutrophil peroxidase system in mice were studied using the method of luminol-enhanced chemiluminescence. Clofibrate and dalargin increased the chemiluminescence of mouse whole blood. Their combined use several-fold potentiated this effect. It is expected that combined use of hypolipidemics and polypeptides will open a new trend in the search for stimulators of oxygen-dependent nonspecific immunity. __________ Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 144, No. 9, pp. 291–292, September, 2007     

红景天苷对急性心肌缺血大鼠心肌细胞凋亡的作用及机制研究

目的：观察红景天苷对急性心肌缺血大鼠心肌细胞凋亡的影响,探讨其可能的分子机制。方法：制备Sprague Dawley大鼠急性心肌缺血模型,随机分为红景天苷高、低剂量组(40 mg/kg、10 mg/kg)、急性心肌缺血组和对照组。原位末端转移酶标记染色法检测心肌组织凋亡情况,免疫印迹法检测心肌组织Bcl-2、Bax、Cytochrome c (Cyt-c)、cleaved caspase-3以及cleaved caspase-9蛋白的表达水平。结果：原位末端转移酶标记染色法显示红景天苷浓度依赖性抑制急性心肌缺血所引起心肌细胞的凋亡;免疫印迹法结果显示,与急性心肌缺血组相比,红景天苷干预后心肌组织中Bcl-2的蛋白表达显著增加,Bax的蛋白表达显著减少,胞浆(cyto)中Cyt-c的蛋白表达水平显著下降,cleaved caspase-3和cleaved caspase-9表达均显著降低。结论：红景天苷具有抗心肌细胞凋亡的作用,其作用机制可能是通过抑制线粒体通路减少细胞的凋亡。本实验为红景天苷可作为临床上治疗缺血性心脏病提供实验依据。     

Antiarrhythmic effect of antibodies to digoxin in experimental myocardial infarction (the arrhythmogenic action of endogenous digoxinlike factor)

I. N. Dzhanelidze Leningrad Emergency Aid Research Institute. I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR. I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad. Institute of Organic Synthesis, Academy of Sciences of the Latvian SSR, Riga. Institute of Endocrinology, Prague, Czechoslovakia. (Presented by Academician of the Academy of Medical Sciences of the USSR A. I. Vorob'ev.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 112, No. 7, pp. 20–22, July, 1991.