血清CEA与β2-微球蛋白联合检测在胃癌诊断中的临床价值

目的探讨血清CEA与β2-微球蛋白联合检测在胃癌诊断中的临床价值。方法检测40例胃癌患者血清CEA、β2-微球蛋白的表达水平,并与胃溃疡组（38例）和健康对照者（42例）对比分析。结果胃癌患者血清CEA和β2-微球蛋白表达水平明显高于胃溃疡组和健康对照组（P〈0．05）,两指标联合检测阳性率明显高于单一指标检测阳性率（P〈0．05）。结论血清CEA与β2-微球蛋白联合检测可明显提高胃癌诊断的阳性率,具有重要的临床应用价值。     

胃癌及术后随访患者血清血管生成素-2含量的临床意义

目的 检测胃癌及术后随访患者血清血管生成素-2 (Ang-2)含量的变化,探讨其临床意义。方法采用ELISA法分别检测158例胃癌、31例慢性萎缩性胃炎、38例胃腺瘤息肉、28例慢性胃炎伴胃溃疡患者及30名健康对照者血清Ang-2与癌胚抗原(CEA)的含量,并检测131例行胃癌根治术患者术后2年血清Ang-2含量的变化,随访其预后。分析胃癌患者血清Ang-2含量与胃癌病理特征的关系。结果 胃癌组血清Ang-2和CEA含量分别为（331.8±64.3）μg/L和(42.6±37.3) μg/L,较正常对照组[（187.4±32.7）μg/L和（4.2±3.1）μg/L]及胃溃疡组[(197.3±35.4) μg/L和(4.5±3.2) μg/L]显著升高（P值均＜0.01）,慢性萎缩性胃炎组Ang-2含量亦明显升高(P＜0.05),而CEA含量无明显变化,胃腺瘤息肉组Ang-2与CEA含量均无明显变化。随访复发组及转移组术前血清Ang-2含量明显高于未复发组(P＜0.05)。未复发组术后1个月血清Ang-2含量降至正常范围,随访2年无明显升高。复发组术后1个月Ang-2含量亦降至正常范围,而术后6个月至2年显著高于未复发组及正常组(P＜0.01)。转移组术后2年Ang-2含量始终显著高于未复发组及正常组(P＜0.01)。未复发组2年存活率显著高于复发组和转移组。胃癌患者血清Ang-2含量变化与胃癌分化程度、TNM病理分期、有无淋巴结转移、有无远处组织转移、浸润深度和肿瘤大小均相关(P＜0.01),与组织学分型、肿瘤部位无相关性(P＞0.05)。结论血清Ang-2可作为一种新的具有一定临床价值的胃癌肿瘤标志物,有助于术后复发、转移的监测和预后评价。     

血清CEA、AFP、CA72-4联合检测评价胃癌分化程度的效能

目的探讨血清癌胚抗原(CEA)、甲胎蛋白(AFP)、糖类抗原(CA72-4)联合检测对胃癌分化程度的评价效能。方法选取2019年2月至2020年9月合肥市第三人民医院收治的92例胃癌患者,并招募92例健康志愿者,分别记为胃癌组和健康组,开展前瞻性研究。两组均采用酶联免疫吸附试验检测血清CEA、AFP和CA72-4水平并进行比较;比较胃癌组不同分化程度患者血清CEA、AFP和CA72-4水平,分析三者联合对胃癌分化程度的评价效能。结果胃癌组血清CEA、AFP和CA72-4水平均高于健康组(P<0.05)。胃癌组未/低分化患者血清CEA、AFP和CA72-4水平均高于中/高分化患者(P<0.05)。血清CEA、AFP和CA72-4水平联合对胃癌组未/低分化评价的灵敏度和AUC分别为100.00%和0.917,均高于单独评价,差异均有统计学意义(P<0.05),特异度与单独评价对比差异均无统计学意义(P>0.05)。结论胃癌患者血清CEA、AFP和CA72-4水平均较高,且未/低分化患者水平更高。三者联合对胃癌分化程度评估效能理想。     

Clinical significance of serum levels of E-selectin,intercellular adhesion molecule-1, and vascular cell adhesion molecule-1 in gastric cancer patients

OBJECTIVES: This study evaluated serum concentrations of soluble cell adhesion molecules in patients with gastric cancer and in healthy control subjects. Our objectives were to correlate these levels with clinicopathological features, established tumor markers, and patient survival, and to assess changes in serum levels of cell adhesion molecules after tumor surgery. METHODS: The serum concentrations of the adhesion molecules E-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) were investigated by ELISA in 57 gastric cancer patients, both before and 7 days after surgery, and in 47 healthy control subjects. RESULTS: Preoperative serum concentrations of ICAM-1 and VCAM-1 in gastric cancer patients were significantly higher when compared with those of healthy controls, whereas there were no differences regarding serum E-selectin levels. Serum levels of E-selectin, ICAM-1, and VCAM-1 correlated significantly with each other. There was a significant association between preoperative levels of all three adhesion molecules and disease stage, gastric wall invasion, lymph node involvement, and presence of distant metastases. Their concentrations decreased significantly after radical resection of the tumor, whereas they remained almost unchanged in patients with unresectable disease. Elevated preoperative serum levels of E-selectin, ICAM-1, and VCAM-1 levels were found in 24.6%, 33.3%, and 28.1% of patients, respectively. Elevated levels of all three molecules were significant prognostic factors for patient survival but not independent of disease stage. CONCLUSIONS: These findings suggest that serum concentrations of E-selectin, ICAM-1, and VCAM-1 may reflect tumor progression and metastasis, and may be clinically useful.     

CEA蛋白、CEAmRNA检测在大肠癌、胃癌早期诊断中的价值及方法探讨

目的研究癌胚抗原（CEA）蛋白、CEAmRNA在大肠癌、胃癌患者血清、癌组织中的表达,为大肠癌、胃癌的早期诊断提供理论依据,并探讨最佳诊断方法。方法选取21例大肠腺癌、20例胃腺癌住院患者和20例胃肠道良性疾病患者,检测其术前血清CEA蛋白表达;用免疫组织化学法和实时荧光定量RT—PCR法检测癌组织、癌旁组织及良性胃肠道疾病组织中CEA蛋白和CEAmRNA表达。结果大肠癌、胃癌组织CEA蛋白及CEAmR-NA的表达均高于癌旁组织及良性胃肠道疾病组织（P〈0．01,或〈0．05）,胃癌组织CEAmRNA表达与血清及组织中CEA表达比较有统计学差异（P〈0．05）;大肠癌组织CEAmRNA表达与血清CEA表达比较有统计学差异（P〈0．05）。结论应用实时荧光定量RT—PCR法检测组织CEAmRNA在大肠癌和胃癌早期诊断中有重要价值。     

Clinical significance of changes in tumor markers, extracellular matrix, MMP-9 and VEGF in patients with gastric carcinoma

BACKGROUND/AIMS: The aim of this study was to evaluate the prognostic significance of some serum tumor marker level, extracellular matrix (ECM), matrix metalloproteinase-9 (MMP-9), vascular endothelial growth factor (VEGF) in patients with gastric cancer. METHODOLOGY: The serum tumor markers included CEA, CA50 and CA19-9, ECM included laminin (LN), hyaluronic acid (HA), and collagen type III and IV were measured in 40 patients with gastric carcinoma and 20 matched healthy controls by radioimmunoassay. MMP-9, VEGF and MVD were measured with immunohistochemical methods and the computer image analyzer. Microvascular density (MVD) in tissues of patients with gastric carcinoma was detected. RESULTS: The levels of CEA, CA50, CA19-9, HA, LN and collagen type IV in the patients with metastasis were significantly higher than those in the patients without metastasis (p < 0.05). The expression of MMP-9 and collage type IV in the patients with metastasis and poorly differentiated carcinomas were significantly higher than those in the patients without metastasis whose tumors were well/moderately differentiated (p < 0.05). CONCLUSIONS: CEA, CA50, CA19-9, HA, LN and collagen type IV levels can be used as a signal of metastasis and disease progression in patients with gastric carcinoma. When a gastric carcinoma expresses a high level of MMP-9 and VEGF with high MVD, the power of infiltration and metastasis of the gastric carcinoma is enhanced.     

胆囊癌患者血清sVEGF-C和CEA水平变化及其与组织病理学特征的关系探讨*

目的 研究血清血管内皮生长因子-C(sVEGF-C)和癌胚抗原(CEA)水平与胆囊癌患者临床和病理学特征的关系。方法 2011年2月～2018年1月收治的93例胆囊癌患者,采用双抗体夹心酶联免疫吸附法检测血清sVEGF-C水平,采用放射免疫分析法检测血清CEA水平,分析血清sVEGF-C和CEA水平与胆囊癌患者临床和病理学特征的关系。结果 不同肿瘤浸润程度(T分期)、肿瘤淋巴转移程度(N分期)、肿瘤远处转移(M分期)的胆囊癌患者血清sVEGF-C和CEA水平相差显著(P＜0.05);中位随访22个月,在93例患者中死亡48例(51.6%),生存45例(48.4%);死亡组血清sVEGF-C水平为(84.9±20.7)pg/mL,显著高于生存组[(56.4±18.9)pg/mL,P＜0.05],血清CEA水平为(138.7±49.6)μg/L,显著高于生存组[(76.8±27.4)μg/L,P＜0.05];多因素Logistic回归分析显示,临床病理分期(OR=6.658,95%CI=1.800～24.632)及血清sVEGF-C(OR=4.005,95%CI=1.292～12.415)和CEA(OR=3.170,95%CI=1.482～6.849)水平是影响胆囊癌患者预后的独立危险因素(P＜0.05)。结论 胆囊癌患者血清sVEGF-C和CEA水平显著升高,可能与不良预后相关,值得积极的深入研究。     

血清肿瘤标志物对老年非小细胞肺癌检测的临床意义

目的探讨血清肿瘤标志物癌胚抗原（CEA）、糖类抗原199（CA199）、鳞状上皮细胞癌抗原（SCC-Ag）、糖类抗原125（CA125）、细胞角蛋白19血清片段抗原21-1（CYFRA21-1）与非小细胞肺癌（NSCLC）患者病理分型以及分期的关系。方法2009年8月至2012年12月住院的90例年龄≥65岁NSCLC患者,全部经组织学诊断确认。应用电化学发光方法检测其血清中5种肿瘤标志物水平。结果血清中5种肿瘤标志物在肺癌患者中表达水平明显高于健康人组（P＜0.05）,在不同性别以及不同年龄段患者中的表达水平差异无统计学意义（P＞0.05）。CEA,CA199,CA125在腺癌中的水平明显高于鳞癌（P＜0.05）;SCC-Ag和CYFRA21-1在鳞癌中的水平明显高于腺癌（P＜0.05）。CYFRA21-1和CA125在N2和N3患者中表达水平明显高于N0和N1患者（P＜0.05）。CEA在有远处转移的患者明显高于无转移的患者（P＝0.041）。CEA和SCC-Ag转移部位越多,其表达越高（P＜0.05）。CEA在Ⅳb患者中表达明显高于Ⅰ+Ⅱ+Ⅲ和Ⅳa患者（P＝0.046）。结论 CEA,CA199,CA125对晚期腺癌的诊断价值较高,SCC-Ag和CYFRA21-1是诊断鳞癌有价值的标志物。5种肿瘤标志物表达与性别、年龄无明显相关。CEA与临床分期正相关,高表达预示分期晚,低表达则对分期参考作用小。CEA和SCC-Ag高浓度应高度警惕有无多部位转移。     

Serum levels of interleukin-2 and tumor necrosis factor-alpha correlate to tumor progression in gastric cancer

BACKGROUND/AIMS: The aim of this study was to assess serum levels of Interleukin-2 and tumor necrosis factor-alpha with disease progression and correlate these levels with CEA and CA19-9 serum levels. METHODOLOGY: Serum levels of interleukin-2 and tumor necrosis factor-alpha were measured in 23 patients with gastric cancer (being 9 stage I or II and 14 stage III or IV) and 10 patients without cancer by ELISA using Predicta Genzyme Diagnostica. The patients were followed for at least 2 years or until death. CEA and CA19-9 were also measured in both groups by ELISA (Abbott Diagnostic). RESULTS: Patients with gastric cancer stage III or IV had elevated levels of these cytokines (P = 0.002 for IL-2 and P = 0.003 for tumor necrosis factor-alpha). There was no difference between the serum levels of tumor necrosis factor-alpha and interleukin-2 in patients with gastric cancer stage I or II and the control group (P > 0.05). We also found no difference among the groups for CEA and CA19-9 (P = 0.17 and 0.72, respectively). Only one gastric cancer patient stage I or II had elevated level of IL-2 and none had elevated levels of tumor necrosis factor-alpha. In the group of patients with gastric cancer stage III or IV, 87.5% of them with elevated levels of tumor necrosis factor-alpha and 75% of them with elevated levels of interleukin-2 died during the follow-up. CONCLUSIONS: We conclude that serum interleukin-2 and tumor necrosis factor-alpha are associated with advanced gastric cancer and that these cytokines might be a useful tumor marker for gastric cancer, being associated with poor prognosis.     

胃病患者血清和胃液胃泌素、胃动素和亮-脑啡肽的研究

用放射免疫法测定胃病患者(胃癌25例,胃溃疡18例和萎缩性胃炎24例)血清和胃液中胃泌素、胃动素和亮-脑啡肽的含量。结果与正常对照组和胃良性疾病比较,胃癌患者血清和胃液胃泌素、胃动素显著升高(P<0.01),血清亮-脑啡肽含量也升高(P<0.05)。揭示测定胃液胃泌素、胃动素和血清亮-脑啡肽对胃癌诊断有一定参考价值。     

cagA阳性幽门螺杆菌感染与老年胃十二指肠病的相关性

目的　探讨老年人幽门螺杆菌 (Hp)cagA基因存在状况及其与老年胃十二指肠病的关系。方法　收集 89例老年和 96例青壮年慢性胃炎、消化性溃疡患者及 3 0例正常人群的血清标本及胃组织标本 ,应用血清学方法检验其Hp cagA阳性菌株感染状况。结果　 89例老年患者中慢性胃炎、胃溃疡、十二指肠溃疡的Hp cagA基因的阳性率分别为 73 .5 % ( 3 8/5 2 )、81.3 % ( 13 /16)和85 .7% ( 18/2 1) ;96例青壮年患者中慢性胃炎、胃溃疡、十二指肠溃疡的Hp cagA基因的阳性率分别为 5 9.3 % ( 3 2 /5 4)、68.8% ( 11/16)和 61.5 % ( 16/2 6) ;对照组Hp cagA阳性菌株感染率为 3 3 .3 %。各疾病组间Hp cagA阳性菌株感染率比较无显著性差异 (P >0 .0 5 ) ,但均高于对照组 (P <0 .0 5 ) ;老年组cagA阳性菌株感染率高于青壮年组 (P <0 .0 5 )。结论　老年患者cagA阳性菌株感染与上述 3种胃十二指肠疾病的发生均密切相关 ,老年患者感染的Hp绝大多数为cagA阳性菌株     

Reduced expression of circulating microRNA-218 in gastric cancer and correlation with tumor invasion and prognosis

AIM:To investigate the expression of microRNA-218(miR-218)in serum from gastric cancer patients and its relationship with clinicopathological characteristics.METHODS:A total of 68 patients with pathologically diagnosed gastric cancer and 56 healthy individuals were recruited to this study.The expression of miR-218was detected in the serum of gastric cancer patients and healthy individuals by quantitative real-time polymerase chain reaction.The clinical data were collectedand analyzed by statistical software.RESULTS:miR-218 was reduced significantly in the serum of gastric cancer patients compared to healthy individuals(1.15±0.08 vs 0.37±0.023;P=0.026).In the gastric cancer group,serum expression of miR-218was lower in patients with metastasis and poorly differentiated cancer compared with non-metastatic and well-differentiated cancer(0.19±0.011 vs 0.45±0.021,P=0.031 and 0.21±0.019 vs 0.49±0.021,P=0.025).Serum miR-218 was found to be significantly associated with gastric cancer metastasis(P=0.003),tumor T stage(P=0.018)and tumor grade(P=0.012).Low serum expression of miR-218 was related to an increase in the stage of gastric cancer.The expression level of miR-218 in the serum was correlated with the3-year survival.Ninety-seven percent of patients with a high level of miR-218 expression survived for 3 years,while only 54%of those with low miR-218 expression survived.CONCLUSION:miR-218 is deregulated in gastric cancer patients and is strongly correlated with tumor stage,grade and metastasis.Serum expression of miR-218 may be a prognostic marker.     

Significance of a novel sucrose permeability test using serum in the diagnosis of early gastric cancer

AIM: To investigate the usefulness of sucrose permeability test using serum in the diagnosis of gastric diseases, with special reference to early gastric cancer (EGC). METHODS: A total of 63 subjects, including 11 patients with gastric ulcer, 20 patients with gastric cancer (13, early; 7, advanced) and 32 healthy controls, were studied. Blood and urine samples were collected repeatedly for 5 h before and after the sucrose loading. Sucrose levels were measured by a newly developed enzymatic method. RESULTS: Serum sucrose levels started to increase 15 min after loading, and peaked at 60 min in the gastric disease groups. The levels for gastric ulcer, EGC and advanced gastric cancer (AGC) at 60 min were significantly higher than that in the healthy controls (26.9±2.4, 34.4±5.0, and 71.8±15.6 vs 7.9±0.7 mol/L, respectively, p<0.01). The cut-off level set at 15.4 mol/L (60 min) offered the best distinction between EGC patients and healthy controls; and the sensitivity and specificity were 92.3% and 93.8%, respectively, while those of the urine method were 76.9% and 93.8%, respectively. CONCLUSIONS: The gastric permeability test using serum is reliable for the detection of EGC, and this test can provide results much earlier than the conventional urine method. This test may offer a useful alternative to more invasive tests for EGC.     

Clinical significance of serum vascular cell adhesion molecule-1 levels in patients with hepatocellular carcinoma

AIM: To evaluate the correlation between serum vascular cellular adhesion molecule-1 (VCAM-1) levels and clinicopathological features in patients with hepatocellular carcinoma (HCC).METHODS: Ninety-six patients who underwent HCC resection were recruited in the study. Preoperative serum levels of soluble VCAM-1 were measured by enzyme-linked immunosorbent assay.RESULTS: Serum VCAM-1 level in HCC patients was inversely correlated with platelet count (r=-0.431,P＜0.001)and serum albumin level (r=-0.279, P＜0.001), and positively correlated with serum bilirubin level (r=-0.379, P＜0.001).Serum VCAM-1 level was not associated with tumor characteristics such as tumor size, venous invasion,presence of microsatellite nodules, tumor grade and tumor stage. Serum VCAM-1 level was significantly higher in HCC patients with cirrhosis compared with those without cirrhosis (median 704 vs 546 ng/mL, P＜0.001). Furthermore, a significantly better disease-free survival was observed in HCC patients with low VCAM-1 level (P=0.019).CONCLUSION: Serum VCAM-1 level appears to reflect the severity of underlying chronic liver disease rather than the tumor status in HCC patients, and low preoperative serum VCAM-1 level is predictive of better disease-free survival after surgery.     

The significance of serum transforming growth factor beta 1 in detecting of gastric and colon cancers

BACKGROUND/AIMS: TGF-beta1 is a growth factor with wide ranging effects on proliferation, differentiation, immune suppression, apoptosis and matrix remodeling. We aimed to clarify the clinical significance of circulating levels of TGF-beta1 as a tumor marker in gastrointestinal tract cancers by comparing it to CEA across a range of parameters such as cancer type and severity. METHODOLOGY: Sera collected from patients with gastrointestinal tract cancers (32 gastric, 36 colon) and from 25 healthy volunteers were analyzed for TGF-beta1 and CEA. Relations between serum TGF-beta1 levels and tumor stage and tumor grade were also evaluated. RESULTS: Mean serum TGF-beta1 levels were higher in patients with gastric or colon cancer compared to the control group (p = 0.001). In both types of cancer there were no differences in TGF-beta1 levels associated with serosal involvement, lymph node involvement, vascular invasion, distant metastasis or tumor size. Mean serum TGF-beta1 levels were also not statistically different across histopathological tumor grades in either type of cancer. The sensitivity of TGF-beta1 was higher in patients with gastric cancer than in patients with colon cancer. TGF-beta1 had greater sensitivity than CEA in gastric cancer patients. CONCLUSIONS: TGF-beta1 has higher sensitivity in gastric and colon cancers. Since it may be increased even in cancer without closed and distant metastasis, TGF-beta1 may be used as a tumor marker and combined with CEA particularly in gastric cancers.     

结直肠癌AIM2和血清CEA表达水平及其临床意义

目的探讨结直肠癌患者黑色素瘤缺乏因子2（AIM2）和血清癌胚抗原（CEA）表达水平及其临床意义。 方法收集辽宁省肿瘤医院2010年1月~2013年3月118例结直肠癌患者的肿瘤组织标本及其50例癌旁正常组织标本,采用免疫组织化学法测定组织中AIM2的表达,回顾性搜集患者临床病理参数及术前通过电化学发光法（ECUA）测定的血清CEA水平。通过相关性分析癌组织中AIM2表达水平和血清CEA水平的相关性,分析两种指标与临床病理参数的关系。采用Kaplan-Meier法对不同AIM2、CEA水平组别进行生存分析。 结果118例肿瘤组织中有42例AIM2呈高表达,有39例癌旁正常组织呈高表达,差异具有统计学意义（χ²=25.295,P<0.001）;结直肠癌患者术前血清CEA阳性率为44.07%（52/118）。Spearman等级相关性分析结果显示,结直肠癌组织AIM2和血清CEA表达呈负相关（r=-0.660,P<0.001）。肿瘤的浸润深度、TNM分期以及淋巴结转移是影响癌组织AIM2表达水平的相关因素（χ²=4.847,7.794,3.961;均P<0.05）;肿瘤大小、TNM分期以及分化程度是影响患者术前血清CEA水平的相关因素（χ²=17.14,5.779,5.293;均P<0.05）。K-M生存分析显示,AIM2高表达组生存时间明显长于低表达组,术前CEA阴性组生存时间明显长于阳性组,AIM2高表达联合CEA阴性患者生存时间明显长于AIM2低表达联合CEA阳性患者,差异具有统计学意义。 结论结直肠癌患者AIM2和血清CEA表达可能与结直肠癌的进展有关,联合分析两个指标有助于评估结直肠癌患者预后。     

血清p53抗体是消化系肿瘤新的标志物

目的　探讨血清p5 3抗体对消化系肿瘤的诊断价值。方法　应用ELISA法检测了食管癌、胃癌、肝癌患者血清中p5 3抗体的浓度。结果　血清p5 3抗体浓度在肿瘤患者和良性疾病对照组中分别为 46 .9pg/mL和 18.6pg/mL(P <0 .0 5 )。以对照组 X± 2S为界值 ( 2 1.3pg/mL) ,则肿瘤组血清p5 3抗体阳性率为 2 1.4%,而对照组则为 5 .6 %(P <0 .0 5 )。肿瘤伴远处转移者p5 3抗体阳性率高于无转移者 (P <0 .0 0 5 ) ,手术后p5 3抗体浓度显著下降 (P <0 .0 5 )。血清p5 3抗体与CEA显著相关 (P <0 .0 0 5 )。血清p5 3抗体对消化系肿瘤诊断的敏感性、特异性、准确性分别为 2 1.4%、94.4%、5 0 %。结论　血清p5 3抗体浓度在消化系肿瘤中异常升高 ,是特异性的肿瘤标志物。     

胃癌患者血清RASSF1A基因启动子区甲基化检测

Ras相关结构域家族1A（RASSF1A）基因是近年发现的新型抑癌基因，其启动子区甲基化可能与胃肠道肿瘤的发生、发展密切相关。目的：检测胃癌患者血清RASSF1A基因启动子区甲基化情况，探讨其在胃癌早期诊断和预后评估中的可能作用。方法：以甲基化特异性聚合酶链反应（MSP）检测47例胃腺癌患者、30例胃良性病变患者和30名健康对照者的血清RASSF1A基因启动子区甲基化情况，其中16例胃腺癌患者同时留取手术切除癌组织、癌旁组织标本以及术前、术后血标本行对照研究。结果：胃腺癌患者血清RASSF1A基因启动子区甲基化率为34．0％（16／47），显著高于胃良性病变患者（3.3％，1／30）和健康对照者（0％）（P〈0．01）。16例胃腺癌组织中5例（31．2％）检测到RASSF1A基因启动子区甲基化，其中4例（80．0％）术前、术后血清标本均检测到RASSF1A基因启动子区甲基化。血清RASSF1A基因启动子区甲基化与胃癌患者性别、年龄、肿瘤分化程度、有无转移以及血清癌胚抗原（CEA）水平均无相关性。结论：血清RASSF1A基因启动子区甲基化检测可望为胃癌的早期诊断和预后判断提供依据。     

Transforming growth factor—β1in invasion and metastasis in colorectal cancer

AIM：To investigate the role of TGFβ1 in invasion and metastasis in colorectal cancer by analysing TGFβ1 correlated wity depth of tumor invasion,stage and metastasis.METHODS:Serum TGFβ1levels were determined in50patients with colorectal cancer and 30healthy volunteers using a TGFβ1 enzyme-linked immunosorbent assay.TGFβ1 expression in primary and lymph node metastatic lesions were detected in 98cases of colorectal cancer by immunohistochemical staining and in situ hybridization.RESULTS:Serum levels of TGFβ1 in patients with colorectal cancer(40&#177;18μg&#183;L^-1)were significantly higher than those in the healthy control group(19&#177;8μg&#183;L^-1),P&lt;0.05.Elevated levels of serum TGFβ1were found in 60%of patients with colorectal cancer when the mean+2s was used as the upper limit of the normal range(35.1μg&#183;L^-1).Increases in serum TGFβ1 levels were significantly asociated with Dukei‘s stage(P&lt;0.05),but there was no significant difference between,Duki‘s stage Bpatients and Dukei‘s stage Cpatients.In the cytoplasm of cancer cells,TGFβ1 was immunostained in37.8%(37/98)of colorectal cancer,and this expression was confirmed by in situ hybridization,Among35cases of colorectal cancer with lymph node metastatic lesions,TGFβ1 positive staining was found in18(51.4%)cases of primary tumor,and 25(71.4%)cases with lymph node metastatic lesions,respectively,Of17cases with no staining in the primary lesion.7(41.2%)casesshowed TGFβ1 staining in the metastatic lesion.Serum TGFβ1 levels and TGFβ1 expression in colorectal cancer tissues were correlated significantly with depth of tumor invasion,stage and metastasis,Patients in stage C-D,T3-T4and with metastasis had significantly higher TGFβ1 levels than patients in stage A-B,T1-T2and without metastasis(P&lt;0.05).CONCLUSION:These results suggest that transforming growth factor-β1 is closely related to the invasion and metastasis of colorectal cancer.It increased the invasive and metastatic potential of tumor by altering a tumor microenvironment.TGFβ1 may be used as a possible biomarke.