口腔颌面部肿瘤患者血清脂质结合唾液酸,总唾液酸水平检测

用生化方法测定口腔颌面部恶性肿瘤５６例，良性肿瘤４７例，并与３５例正常人作对照。检测肿瘤患者血清脂质结合唾液酸（ＬＳＡ），总唾液酸（ＴＳＡ）的含量。结果：恶性肿瘤组中ＬＳＡ、ＴＳＡ高于良性肿瘤组和正常对照组（Ｐ＜０．０１）；良性肿瘤组与正常对照组无显著差异（Ｐ＜０．０５）；ＬＳＡ、ＴＳＡ的值与口腔颌面部恶性肿瘤的ＴＮＭ分期之间有一定的关系；其含量水平升高或明显下降对判定疗效预后有价值。在鉴别诊断方面有重要意义。     

口腔颌面肿瘤患者血清唾液酸的测定

本文对３８例正常人及２９例口腔颌面部肿瘤患者血清唾液酸水平进行了分析,结果显示：口腔颌面部肿瘤患者血清唾液酸的含量明显高于正常人（ｐ＜０．０１）,且涎腺混合瘤（临界瘤）、口腔颌面部恶性肿瘤患者血清唾液酸的含量均有显著升高（ｐ＜０．０１）,但这两组之间无明显差异（ｐ＞０．０５）．肿瘤血清唾液酸检测的阳性率约为８３％,提示血清唾液酸的升高可作为口腔颌面部肿瘤诊断的辅助指标之一。     

口腔颌面部肿瘤患者血清CEA测定结果的初步观察

本文介绍通过采用ＥＬＬＩＳＡ法对６１份正常人血清及４９份口腔颌面部肿瘤患者血清进行癌胚抗原（ＣＥＡ）含量测定，得出各组ＣＥＡ含量结果（ｕｇ／Ｌ）。经统计学处理，正常人组（Ｘ±ＳＤ，２．９４±１．５５）与良性肿瘤组（Ｘ±ＳＤ，４．１２±２．００）对比，Ｐ＜０．０１。正常人组与恶性肿瘤组（Ｘ±ＳＤ，５．７５±２．７７）对比，Ｐ＜０．０１。良性肿瘤组与恶性肿瘤组对比，Ｐ＜０．０５．血清ＣＥＡ含量测定阳性率：良性肿瘤组为４．８％，恶性肿瘤组为２５％。由此可以推测口腔颌面部恶性肿瘤患者血清ＣＥＡ含量明显高于正常人和良性肿瘤患者。本文实验结果提示，在口腔科临床开展血清ＣＥＡ含量的测定，对诊断颌面部恶性肿瘤具有一定的辅助作用。     

口腔颌面部恶性肿瘤患者血清唾液酸测定

口腔颌面部恶性肿瘤患者血清唾液酸测定邢汝东，陈瑞梅，王植三，李长贵，唐全勇，蒋崇槟，张玉琢唾液酸（Ｓｉａｌｉｃａｃｉｄ，ＳＡ）是细胞膜的重要组成部分，参与细胞表面的多种生理功能，与细胞的恶变、癌转移及浸润等有密切关系。我们曾对口腔颌面部恶性肿瘤患者血...     

ＦＳＧＦ在口腔颌面部肿瘤的检测及其临床意义

目的：研究恶性肿瘤特异生长因子（Ｔumor Specific Growth Factor,TSGF)在口腔颌面面部肿瘤检测。结果：恶性肿瘤组ＴＳＧＦ水平高于正常对照组和良性肿瘤组（Ｐ＜０．０１）,在恶性肿瘤组中第三期ＴＳＧＦ值最高恶性肿瘤组阳率达达７７．７８％。结论：血清ＴＳＧＦ检测对口腔颌面部肿瘤的诊断和预后判断有一定意义,可作为一种有价值的肿瘤标记物应用于临床。     

口腔颌面部肿瘤患者血清可溶性白细胞介素─2受体水平的研究

作者用双抗体夹心法酶联免疫吸附试验（ＥＬＩＳＡ法）检测了４６例颌面部肿瘤患者血清可溶性白细胞介素－２受体（ＳＩＬ－２Ｒ）水平、并与２５例正常健康人进行对照比较。结果显示：口腔颌面部恶性肿瘤患者血清ＳＩＬ－２Ｒ水平显著高于颌面部良性肿瘤患者血清ＳＩＬ－２Ｒ水平（Ｐ＜０．０ｌ）及正常健康人血清ＳＩＬ－２Ｒ水平（Ｐ＜０．０ｌ），而颌面部良性肿瘤患者血清ＳＩＬ－２Ｒ水平与正常健康人血清ＳＩＬ－２Ｒ水平之间无显著性差异（Ｐ＞０．０５）。本研究表明、血清ＳＩＬ－２Ｒ有可能作为颌面部恶性肿瘤的诊断、疗效考核及预后判断的一个辅助监测指标。     

血清唾液酸含量测定对口腔颌面部恶性肿瘤的诊断价值

血清唾液酸含量测定对口腔颌面部恶性肿瘤的诊断价值安银东王虹杨聚才张浩（第四军医大学口腔医学院检验科７１００３２）～～～～～～～～～～～～～～～～～～～～～～～许多研究表明，血清唾液酸（ＳＡ）含量增高与恶性肿瘤相关，并与病情的严重程度成正相关。血清ＳＡ...     

口腔颌面部肿瘤患者血清可溶性白细胞介素—2受体水平的研究

作者用双抗体夹心法酶联免疫吸附试验（ＥＬＩＳＡ法）检测了４６例颌面部肿瘤患者血清可溶性白细胞介素－２受体（ＳＩＬ－２Ｒ）水平，并与２５例正常健康人进行对照比较，结果显示，口腔颌面部恶性肿瘤患者血清ＳＩＬ－２Ｒ水平显著高于颌面部良性肿瘤患者血清ＳＩＬ－２Ｒ水平（Ｐ〈０．０１）及正常健康人血清ＳＩＬ－２Ｒ水平（Ｐ〈０．０１），而颌面部良性肿瘤患者血清ＳＩＬ－２Ｒ水平与正常健康人血清ＳＩＬ－２Ｒ水平之间     

口腔颌面部恶性肿瘤患者血清唾液酸测定及其临床价值

本文对81例口腔颌面部恶性肿瘤患者进行血清唾液酸测定。血清唾液酸含量恶性肿瘤患者高于正常人和良性肿瘤患者,Ⅲ、Ⅳ期患者高于Ⅰ、Ⅱ期患者。唾液酸含量下降后再升高或持续保持在高水平,提示有复发、转移的可能或预后不良,如一直保持在正常水平则提示病情平稳。     

口腔颌面部肿瘤患者血清唾液酸测定及其临床意义

口腔颌面部肿瘤患者血清唾液酸测定及其临床意义宋萌，郭洁之，马钧，龚武明唾液酸（ｓｉａｌｉｃａｃｉｄ，ＳＡ）是细胞膜的重要组成成份。它参与细胞表面的多种生理功能，其含量和成份的变化直接影响到细胞膜的生物学特性和功能，它在恶性肿瘤的生物行为中起重要的作用...     

唾液SA、CEA测定在口腔鳞状细胞癌诊断中的意义

目的　通过对唾液中癌胚抗原 (CEA)和唾液酸 (SA)含量的测定和分析 ,探讨唾液中CEA和SA含量在口腔颌面部鳞状细胞癌诊断中的意义。方法　分别采用ELLISA法酶法 ,检测了 2 2例口腔鳞状细胞癌患者、2 2例口腔良性肿瘤患者、4 0例正常人唾液中的CEA和SA含量。结果　经过对测定数据进行统计学处理 ,SA含量在正常人组与良性肿瘤组对比 ,P >0 .0 5 ;正常人与鳞状细胞癌组对比 ,P <0 .0 1。良性肿瘤与鳞状细胞癌组对比 ,P >0 .0 5 ;CEA含量在正常人组与良性肿瘤和鳞状细胞癌组对比 ,P <0 .0 1,而鳞状细胞癌和良性组对比P >0 .0 5。结论　联合检测口腔颌面部鳞状细胞癌唾液CEA和SA含量明显高于正常人和良性肿瘤患者 ,口腔科临床开展唾液CEA和SA的测定 ,对诊断颌面部鳞状细胞癌具有一定的辅助作用     

牙菌斑体外产酸方法测定龋活性研究

目的 探讨龋活性结果与龋病现状之间的关联性。方法 将龋活性试验应用于幼儿匀儿童１５名调查研究。结果 龋生试验结果人群分布比例,（＋）最高占３７％,其次为（＋＋）占２８％,（＋＋＋）占１２％,（－）占２３％,不同龋活性结果之间ｄｆｌ、ｄｆｓ有显著性差异（Ｐ〈０．００１）并且ｄｔ,ｄｓ、ｄｆｔ、ｄｆｓ,ＣＳＩ龋病现状随龋活性结果增加有加重的趋势。结论 牙菌斑龋活性试验是对患者口腔健康教育的重要手段。     

Flow rate,amylase activity,and protein and sialic acid concentrations of saliva from children aged 18, 30 and 42 months attending a baby clinic

Whole-saliva samples were collected under slight suction from children enrolled in a dental-care programme at a baby clinic and analysed according to sex. The children were allocated to three age groups: 18, 30 and 42 months. No distinct sex difference was found for flow rate; there was a statistically non-significant tendency for an increased rate in females. In the males the flow rates of the 30- and 42-month groups were higher than those of the 18-month group; a similar result was obtained when findings for both sexes were combined. There was no difference in salivary protein concentration between the sexes or the age groups. Amylase activity increased significantly in males from 18 to 30 months of age. Sialic acid concentration (micromol/mg protein) showed a sex difference for the 18-month group; it was 38% higher in males than in females, and 48% higher than in the males of 42 months. This trend toward a reduction in sialic acid concentration with age suggests a reduced synthesis and secretion of glycoproteins.     

正常成人颞颌关节的CT和MRI影相分析

本文对10名正常成人双侧TMJ进行了CT扫描,其中3名作了MRI扫描。对所有CT片作了TMJ骨性结构的三维分析,观察了正常人髁状突和关节凹的形态类型,并发现双侧形态均为同型,双侧髁状突运动范围一致。同时,还用CT软组织窗位相(闪烁相)对关节盘进行了正中(牙合)位和尽力开口位的观察,并用MRI对其结果加以证实。本研究所得结果较其它方法准确,更具有可比性。因为本研究选用CT对双测TMJ进行同时扫描,消除了以往X光片需分侧和多种投照检查所造成的误差。     

变形链球菌的适应性耐酸能力

目的 研究变形链球菌的适应性耐酸能力及其相关生理变化。方法 通过测定在各种酸性pH值培养基中预酸化两小时后，能否使变形链球菌UA159抵抗致死性pH值的杀伤而存活及存活率，来测定适应性耐酸能力的有无及大小；氯霉素对该能力的影响可用来判断有否新蛋白的产生；用pH下降实验来测定产酸的改变。结果 变形链球菌UA159属强适应性耐酸能力类菌株，最大生存率(9．89％)出现在pH5．5预酸化组。适应性耐酸能力的产生与蛋白质的合成密切相关。酸适应能增加细菌的产酸能力。结论 在亚致死性pH值中生长能引发链球菌的某些生理变化。产生适应性耐酸能力，抵抗酸性环境的杀伤作用。     

4D microstructural changes in dentinal tubules during acid demineralisation

ObjectiveDental erosion is a common oral condition caused by chronic exposure to acids from intrinsic/extrinsic sources. Repeated acid exposure can lead to the irreversible loss of dental hard tissues (enamel, dentine, cementum). Dentine can become exposed to acid following severe enamel erosion, crown fracture, or gingival recession. Causing hypersensitivity, poor aesthetics, and potential pulp involvement. Improving treatments that can restore the structural integrity and aesthetics are therefore highly desirable. Such developments require a good understanding of how acid demineralisation progresses where relatively little is known in terms of intertubular dentine (ITD) and peritubular dentine (PTD) microstructure. To obtain further insight, this study proposes a new in vitro method for performing demineralisation studies of dentine.MethodsAdvanced high-speed synchrotron X-ray microtomography (SXM), with high spatial (0.325 μm) and temporal (15 min) resolution, was used to conduct the first in vitro, time-resolved 3D (4D) study of the microstructural changes in the ITD and PTD phases of human dentine samples (∼0.8 × 0.8 × 5 mm) during 6 h of continuous acid exposure.ResultsDifferent demineralisation rates of ITD (1.79 μm/min) and PTD (1.94 μm/min) and their progressive width-depth profiles were quantified, which provide insight for understanding the mechanisms of dentine demineralisation.SignificanceInsights obtained from morphological characterisations and the demineralisation process of ITD and PTD during acid demineralisation would help understand the demineralisation process and potentially aid in developing new therapeutic dentine treatments. This method enables continuous examination of relatively large volumes of dentine during demineralisation and also demonstrates the potential for studying the remineralisation process of proposed therapeutic dentine treatments.     

Oral lichen planus: an immunohistochemical study of heat shock proteins (HSPs) and cytokeratins (CKs) and a unifying hypothesis of pathogenesis

The expression of heat shock proteins HSP60 and HSP70 and cytokeratins CK1/10 and CK7/18 were compared in epithelium of oral lichen planus (OLP) lesions and oral fibromas using an avidin-biotin-peroxidase complex (ABC) immunohistochemical method. An immunostaining intensity distribution (IID) index was developed to assess staining intensity and the proportion of positively stained cells in different layers of the epithelium. The expression of HSP60 in the basal layer was significantly higher in OLP than in fibromas. No difference in HSP70 expression was evident between OLP and fibromas. The expression of CK1/10 in the epithelial basal and suprabasal layers was significantly higher in OLP than in fibromas. There was no demonstrable staining for CK7/18 in either OLP or fibromas. A significant correlation was evident between the expression of HSP60 and CK1/10 in the basal epithelial cells in OLP. The findings support a role for HSP60 in the pathogenesis of OLP. A unifying hypothesis of the pathogenesis of OLP, involving two sequential immune reactions, is proposed.     

牙周韧带细胞与骨髓基质细胞表面抗原表达的比较

目的：比较骨髓基质细胞与正常人牙周韧带细胞表面抗原的表达情况,探讨牙周韧带细胞与骨髓基质细胞的关系。方法：体外培养人牙周韧带细胞及骨髓基质细胞,采用免疫组化SP法检测细胞中CD14、CD44、CD105、CD34的表达,并进行图像分析。结果：与骨髓基质细胞相似,人的牙周韧带细胞上表达CD44、CD105,不表达CD34、CD14结论：人的牙周韧带细胞与骨髓基质细胞在表面抗原特性上有相似性。     

性激素受体在牙龈瘤组织中的表述

本文采用免疫组织化学方法（ＡＢＣ法）对３４例牙龈瘤和２０例正常牙龈组织进行雌激素受体（ＥＲ）和孕激素受体（ＰＲ）检测．结果发现,牙龈瘤ＥＲ和ＰＲ的阳性率（８２％和６２％）明显高于正常牙龈组织（１５％和２５％）,而且阳性程度亦明显高于正常组（ｐ＜０．００１）．进一步研究发现,纤维型牙龈瘤ＥＲ和ＰＲ阳性率高于肉芽肿型牙龈瘤（ｐ＜０．０５）;女性正常牙龈组织中ＥＲ和ＰＲ的阳性率略高于男性．结果说明：正常牙龈组织中存在有特异性的性激素受体,牙龈瘤组织中ＥＲ和ＰＲ的明显增高可能说明在肿瘤的生长过程中存在着一定程度的性激素依赖性．