基于径向基函数神经网络的组合模型在煤工尘肺发病工龄预测中的应用

目的：研究径向基函数（ RBF）神经网络与多重线性回归的组合模型在煤工尘肺发病工龄预测中的性能优劣。方法：采用RBF神经网络模型与多重线性回归模型对研究数据进行分析,对2模型进行加权拟合,采用均方根误差、均方误差、平均相对误差对模型的预测性能进行评价。结果：多重线性回归模型、RBF神经网络模型和组合模型真实值与预测值比较,差异均无统计学意义（t配对＝1．552、0．231、0．155,P均＞0．05）。多重线性回归模型、RBF神经网络模型和组合模型的均方根误差分别为（1．63±0．11）、（2．45±0．19）和（0．59±0．07）（F＝26．141, P＜0．001）,均方误差分别为（2．6569±0．2412）、（5．9867±0．3804）和（0．3483±0．0653）（F ＝49．678, P＜0．001）,平均相对误差分别为（7．15±0．82）％、（15．39±1．25）％和（3．68±0．59）％（F＝35．282,P＜0．001）。结论：在煤工尘肺发病工龄的预测中,组合模型预测性能优于单一模型。     

我校基础部两教授创建的疼痛模型得到国际公认

由我校神经生物学教研室胡三觉教授与邢俊玲副教授，于1998年创建的背根节慢性压迫实验模型，被美国科学院院士、《PAIN》杂志主编A．I．Basbaum与国际疼痛研究学会主编M．C．Bushnell联合编著的《Science of Pain》（2009年版）选用．该书详尽介绍了当代疼痛领域的基础知识与前沿进展，是公认的疼痛学领域权威专著．书中选择介绍了12个国际通用的神经病理性疼痛模型，只有背根节慢性压迫实验模型属于我国学者自主创建．这一模型被世界著名专著选用，标志着该模型已得到国际公认．     

大鼠辐射损伤模型的复制

为复制辐射损伤大鼠模型，将40只SD大鼠随机分成模型组和对照组，每组雌雄各半。用60CoY射线小剂量多次累积全身照射模型组大鼠，每次0．3Gy，共8次．累积剂量为2．4Gy。结果，模型组大鼠的白细胞总数比对照组下降35．5％（P＜0．01），骨髓细胞染色体畸变率明显上升（P＜0．01），表明复制成功。     

不同形态桩核三维有限元模型建立

目的提出一种快速建立具有复杂形态结构的上前牙不同形态桩核三维有限元模型,并利用有限元分析方法验证所建模型的有效性。方法采用螺旋CT扫描离体上颌中切牙．利用“计算机颅颌骨三维识别系统”软件和unigraphics(UG)软件建立上颌中切牙实体模型及锥形、柱形柱核模型,并将后者插入前者实体模型．分别建成锥形、柱形桩冠三维有限元模型．对锥形桩冠模型进行网格划分并设定边界条件、加载条件,计算分析：结果本实验所建模型与实体模型具有良好的几何相容性,模型受力后应力分布符合预期结果．结论所建模型能较真实地模拟实体．实验方法具有准确快速的特点．     

ARMA模型在非意外死亡率动态预测中的应用

目的：探讨ARMA模型在某县区死亡率动态分析中的应用,分析和预测其非意外死亡率的动态发展趋势。方法：对该地2008年1月-2012年9月的逐月死亡率进行ARMA模型拟合,采用2012年10-12月的实际月死亡率验证模型的预测效果。结果：MA（1）模型较好地拟合了该地区既往时间段上的死亡率序列,模型残差为白噪声（P〉0．05）,模型参数MAl,1—0．470,有统计学意义（t=-4．010,P=O．000）,AIC=431．719,SBC=435．907,模型数学函数式为：X1=35．07217＋（1＋0．4757B）εt预测得到的2012年10—12月死亡率为32．65／10万、35．07／10万、35．07／lO万,2013年1月死亡率为35．07／10万,预测误差为12．25％。结论：ARMA模型可以较好地拟合死亡率的时间变化趋势,并用于预测未来的死亡率,是一种短期预测精度较高的预测模型。     

经颅注射无水乙醇建立大鼠脑损伤模型初步探讨

目的探讨如何经颅注射无水乙醇建立大鼠脑损伤模型。方法10只SD大鼠,采用经颅注射无水乙醇建立大鼠脑损伤模型。结果大鼠脑损伤模型成功率为100％。10只脑损伤大鼠,均存活。10只假手术组大鼠也全部存活。实验组及假手术组术后4周体重无显著差异（376．1±11．5g vs 353．2±10．3g;P=1．534）。日饮食量也无显著差异（23．4±3．1gVS19．8±2．3g;P=0．723）。通过脑核磁共振、病理HE染色、及脑组织切片TTC染色检测可以证实建模成功。结论经颅注射无水乙醇建立大鼠脑损伤模型较传统大鼠脑损伤模型的建立操作简单、模型成功率高,结果可靠。     

葛根黄酮对乙醇性肝损伤的保护作用

目的探讨葛根黄酮对乙醇引起的肝损伤模型大鼠肝脏的保护效果。方法采用乙醇建立大鼠肝损伤模型,测定肝组织的丙二醛（MDA）、甘油三酯（TG）及还原型谷胱甘肽（GSH）含量,并观察肝脏的病理组织学改变。结果受试样品高、中、低剂量组大鼠肝组织的MDA含量均值分别为18．6、18．7、18．4nmol／g（肝组织）,均低于肝损模型组的32．4nmol／g;TG含量分别为2．27、2．74、2．69mmol／100g（肝组织）,均低于肝损模型组的4．97mmol／100g;而GSH含量分别为351．2、336．9、360．3μmoL／100g（肝组织）,均高于肝损模型组的276．4μmoL／100g;各剂量组大鼠肝脏病理改变评分均值分别为51．4、61．3、102．6分,均低于肝损模型组的206．6分。结论葛根黄酮对乙醇引起的肝损伤具有保护作用。     

维生素D受体基因多态性与肺结核关系的Meta分析

目的 分析维生素D受体基因多态性与肺结核易感性的病例对照研究的结果。方法应用Meta—analysis，以Fleiss模型和DerSimonian and Nan Laird模型（D—L模型）对6项研究结果进行数据处理。结果累计病例841例，对照1419例，采用Fleiss模型计算的合并OR值ff vs FF为1．20（0．84-1．71），Ff vs FF为0．99（0．81～1.21），D—L模型计算的合并OR值ff vs FF为1．12（0．67-1．86），Ff vs FF为0．99（0．80-1.23）。失效安全数小于1。结论尚不能认为维生素D受体基因多态性是肺结核易感性的影响因素。     

建立兔腹主动脉血管再狭窄模型的实验研究

目的探讨建立兔血管再狭窄的简单模型方法．方法总计67只新西兰兔接受了经股动脉途径行球囊损伤腹主动脉术，术后4周处死兔，观察血管再狭窄模型制作情况．结果所有兔均完成了手术，59只存活至实验结束，平均手术时间（26±7）min，成功造成兔血管再狭窄模型，平均相关狭窄达（63．3±6．4）％．结论本方法成功建立了兔血管再狭窄实验模型，手术成功率高，操作时间少，存活率高，模型稳定，重复性好，值得进一步研究应用．     

青蒿琥酯对肺纤维化大鼠热休克蛋白47表达的影响

目的：探讨青蒿琥酯对肺纤维化大鼠热休克蛋白47（Hsp47）表达的影响。方法雄性 SD 大鼠30只,分为对照组、模型组和青蒿琥酯干预组（简称干预组）。对照组一次性气管内滴入生理盐水约0．4 mL,模型组和干预组一次性气管内滴入博来霉素5 mg／kg。对照组和模型组次日每天腹腔注射生理盐水1．0 mL。干预组每天腹腔注射青蒿琥酯10 mg／100 g。记录0、14、28 d 大鼠的体质量。各组大鼠于第28天处死,计算肺系数,取肺组织进行苏木精-伊红（HE）染色、Masson 染色、羟脯氨酸检测和Hsp47、Ⅰ型胶原 RT-PCR 和 Western blot 检测。结果（1）对照组大鼠体质量增长明显高于其他两组（P ＜0．05）。（2）肺系数：模型组[（12．31±1．89）mg／g]＞干预组[（8．54±0．67）mg／g]＞对照组[（4．81±0．38）mg／g],P ＜0．05。（3）Ashcroft 评分：模型组[（5．70±1．09）分]＞对照组[（3．08±0．56）分],模型组[（5．70±1．09）分]＞干预组[（4．01±1．25）分],P ＜0．05;对照组和干预组差异无统计学意义（P ＞0．05）。（4）羟脯氨酸含量：模型组（620．33±66．16）μg／g＞对照组（379．00±35．51）μg／g,模型组（620．33±66．16）μg／g＞干预组（429．00±36．51）μg／g,P ＜0．05;对照组和干预组间差异无统计意义（P ＞0．05）。（5）Hsp47 mR-NA：模型组高于对照组,模型组和干预组比较差异无统计意义（P ＞0．05）。Ⅰ型胶原 mRNA：模型组＞干预组＞对照组（P ＜0．05）。（6）Hsp47蛋白表达水平模型组明显高于对照组和干预组。结论青蒿琥酯可能通过下调 Hsp47表达水平来抑制胶原的合成进而减轻肺纤维化。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.