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胆宁片对高脂饮食大鼠非酒精性脂肪性肝炎模型形成的影响 总被引:4,自引:0,他引:4
目的 高脂饮食建立大鼠非酒精性脂肪性肝炎模型 ,观察中药胆宁片对造模形成的影响。方法 以连续16周的高脂饮食建立大鼠脂肪性肝炎模型 ,治疗组大鼠从高脂饮食 8周起加用胆宁片治疗。测定血脂和血清转氨酶 ,苏木精 伊红染色观察肝脏病理改变 ,并以正常饮食大鼠作为对照。结果 与正常组 (n =6)相比 ,模型组大鼠 (n =12 )血清TC、LDL C、ALT、AST水平显著升高 ,所有造模肝组织均呈现脂肪性肝炎改变 ,模型复制率为 10 0 %。与模型组相比 ,治疗组大鼠 (n =12 )血清TC、LDL C以及肝指数 ( 3 .91± 0 .5 3对 3 .48± 0 .2 9,P <0 .0 5 )显著下降 ,肝组织炎症活动计数显著降低 ( 5 .83± 2 .0 2对 4.2 3± 0 .64 ,P <0 .0 5 ) ,但仍显著高于正常对照组 ,并且血清转氨酶和肝脂肪变性程度在模型组和治疗组之间差异无显著性。结论 持续 16周的高脂饮食可成功复制大鼠非酒精性脂肪性肝炎模型 ,胆宁片对模型大鼠血脂紊乱和肝组织炎症改变有一定改善作用。 相似文献
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节制饮食对大鼠非酒精性脂肪性肝炎的治疗作用 总被引:4,自引:0,他引:4
非酒精性脂肪性肝病 (nonalcoholicfattyliverdiseases ,NAFLD)可发展为肝纤维化和肝硬化 ,且经过非酒精性脂肪性肝炎 (nonalcoholicsteatohepatitis ,NASH )这一病理阶段[1 3 ] ,因而早期干预是必要的。然而至今尚无治疗NAFLD的特效药物 ,因此作为基本治疗的饮食疗法在NAFLD治疗中的作用越发受到重视。本研究将观察节制饮食对NASH的治疗作用。材料与方法一、材料雄性SD大鼠 2 0只 ,购自西普尔 必凯公司 ,体重 14 0~160 g ,正常喂养 1周后 ,通过高脂饮食 12周建立NASH大鼠模型 ,随机分为 2组 :模型组 10只继予高脂饮食喂养 ;… 相似文献
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熊去氧胆酸对高脂饮食大鼠脂肪性肝炎形成的影响 总被引:5,自引:1,他引:4
目的 探讨熊去氧胆酸对高脂馀食诱发的大鼠非酒精性脂肪性肝炎模型形成的影响。方法 29只SD大鼠随机分为3组:模型组予高脂饮食饲养;治疗组在高脂饮食饲养2周后同时加用熊去氧胆酸;正常组予标准饮食饲养,12周处死,结果 所有造模大鼠均出现肥胖、高脂血症和血清转氨酶升高,伴典型的脂肪性肝炎;与模型组相比,治疗组仅血清转氨酶、肝组织脂肪变和炎症坏死等指标有改善趋势,但两组间并无统计学差异。结论 熊去氧胆酸对高脂饮食诱发的肥胖、高脂血症性脂肪性肝炎可能并无防治作用。 相似文献
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目的:观察改良蛋氨酸胆碱缺乏饮食(MCD)建立的脂肪性肝炎大鼠模型肝脏的脂肪变性和炎症情况.方法:大鼠26只随机分为4组,分别喂养MCD饮食和胆碱添加饮食(CS).3-8wk后处死,肝脏标本在甲醛中固定和石蜡包埋.HE染色和Masson染色后对脂肪变性、炎症和纤维化进行评分.血清ALT,AST,CH,LDL等指标通过生化分析仪进行测定.结果:MCD喂养组3wk可以看见肝脂肪变性和炎症,肝指数显著增加,ALT和AST显著升高,8wk可见纤维形成.结论:可通过改良的蛋氨酸胆碱缺乏(MCD)饮食建立非酒精性脂肪肝动物模型. 相似文献
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[目的]观察荷丹片对非酒精性脂肪性肝炎(NASH)大鼠脂代谢的干预作用。[方法]雄性SD大鼠40只,随机分为4组:正常组、模型组、阳性对照组和治疗组。除正常组外,其余各组给予高脂饲料喂养,8周后开始药物灌胃干预,治疗4周后处死全部动物,应用全自动生化分析仪进行血清生化指标检测,油红0染色光镜下观察大鼠肝细胞脂肪变性情况。[结果]与模型组相比,荷丹片治疗后可明显减低NASH大鼠血清中丙氨酸氨基转移酶、天冬氨酸转氨酶和三酰甘油水平,升高高密度脂蛋白胆固醇水平。光镜观察荷丹片能明显缓解NASH大鼠肝细胞脂肪变性。[结论]荷丹片对高脂饮食诱导的大鼠NASH有一定的治疗作用。 相似文献
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吴婷 《内科急危重症杂志》2023,29(3):236-241
目的:研究高脂饮食对病毒性肝炎小鼠肝内细胞因子表达的影响。方法:选取C3H/HeN小鼠共152只,随机分为标准饮食组(32只)、高脂饮食组(32只)、标准饮食感染组(44只)、高脂饮食感染组(44只)。标准饮食组及标准饮食感染组持续予以标准饮食喂养,高脂饮食组及高脂饮食感染组持续予以高脂饮食喂养。喂养第12周末,标准饮食感染组及高脂饮食感染组予以腹腔注射3型鼠肝炎病毒(MHV-3)诱导病毒性肝炎。于感染后不同时间点,采用定量逆转录聚合酶链式反应(PCR)检测肝内肿瘤坏死因子(TNF-α)、白介素(IL)-1β、IL-6、IL-17A表达。结果:高脂饮食喂养12周诱导小鼠出现代谢异常。感染后第4天,标准饮食感染组小鼠肝内仅IL-6表达上调,而高脂饮食感染组TNF-α、IL-1β、IL-6、IL-17A表达上调(P均<0.05)。感染后第8天,标准饮食感染组及高脂饮食感染组小鼠肝内TNF-α、IL-1β、IL-6、IL-17A表达上调,且高脂饮食感染组表达水平显著高于标准饮食感染组(P均<0.05)。感染后第12天,标准饮食感染组小鼠肝内TNF-α、IL-17A表达回落至未感染... 相似文献
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目的:观察荷丹片对非酒精性脂肪性肝炎(NASH)大鼠的干预作用。方法:雄性SD大鼠40只,随机分为4组:正常组、模型组、阳性对照组和荷丹片治疗组。除正常组外,其余各组大鼠给予高脂饲料喂养,造模8周后开始药物灌胃干预,治疗4周后处死全部动物,应用全自动生化分析仪、放免法等进行血清生化和肝组织生化指标检测,并在HE染色光镜下观察大鼠肝组织病理形态学改变。结果:与模型组相比,荷丹片治疗后可明显降减低大鼠血清及肝组织中胰岛素抵抗相关指标:血糖(Glu)、胰岛素(INS)及游离脂肪酸(NEFA)水平,增加肝组织中超氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶(GSH-Px)含量。光镜观察发现荷丹片能明显缓解NASH大鼠肝细胞脂肪变性,减轻肝小叶中炎性细胞浸润。结论:荷丹片能够通过缓解胰岛素抵抗、调节抗氧化功能等途径降低NASH大鼠肝细胞中的脂质蓄积,从而改善肝功能。 相似文献
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Guang-De Zhou Mei-Rong Li Jing Zhang Deng Pan Su-Xian Zhao Jian-Fa Yang 《Scandinavian journal of gastroenterology》2013,48(11):1371-1377
Objective. Currently, no agent has been conclusively demonstrated to prevent the progression of non-alcoholic steatohepatitis (NASH). Chitosan, a natural product derived from chitin, was thought to possess hypocholesterolemic properties. The aim of this study was to evaluate the potential effects of chitosan on nutritional steatohepatitis in rats. Material and methods. Rats were fed with a high fat diet for 4 weeks to develop NASH that was confirmed by liver biopsy, and then 4 weeks of chitosan was given. Serum chemistry and liver histology were assessed and the steatoinflammatory mechanisms were studied. Results. Chitosan significantly protected against high fat diet-induced hepatic steatohepatitis. This effect was associated with repressed serum levels of total protein (TP), globulin (GLO), alanine aminotransferase (ALAT), alkaline phosphatase (ALP), γ-glutamyl transpeptidase (GGT), total cholesterol (TC) and low-density lipoprotein (LDL). Chitosan elevated the serum levels of high-density lipoprotein (HDL) and the ratio of albumin to globulin. Furthermore, increased TNF-α, lipoemia, hyperinsulinemia, hyperleptinemia and hypoadiponectin in NASH were significantly ameliorated by treatment with chitosan. Conclusions. Chitosan effectively attenuated the steatohepatitis induced by a high fat diet. The therapeutic effect of chitosan on NASH may be activated through exerting an influence on adipokines. 相似文献
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Takeharu Asano Kiyotaka Watanabe Naoto Kubota Toshiaki Gunji Masao Omata Takashi Kadowaki Shin Ohnishi 《Journal of gastroenterology and hepatology》2009,24(10):1669-1676
Background and Aim: Low levels of serum adiponectin have been reported to be associated with obesity, diabetes, and non-alcoholic steatohepatitis (NASH), as well as several malignancies. Adiponectin knockout (KO) mice have been reported to cause insulin resistance and neointimal formation of the artery. We used adiponectin KO mice fed a high fat (HF) diet, and investigated the effect of adiponectin on the progression of steatohepatitis and carcinogenesis in vivo .
Methods: Adiponectin KO mice and wild type (WT) mice were fed a HF diet or normal chow for the periods of 24 and 48 weeks. The HF diet contained 60% of calories from fat.
Results: The adiponectin KO mice on the HF diet showed obesity, marked elevation of serum transaminase levels, and hyperlipidemia. At 24 weeks, hepatic expression of tumor necrosis factor-α and procollagen α (I) was higher in KO mice as compared with WT mice. At 48 weeks, liver triglyceride contents in KO mice on normal chow were significantly higher than those in WT mice. Hepatocyte ballooning, spotty necrosis, and pericellular fibrosis around central veins were observed in KO mice on the HF diet. The pericellular fibrosis was more severe in KO mice on the HF diet than that in WT mice (1.62% vs 1.16%, P = 0.033). Liver adenoma and hyperplastic nodules developed in a KO mouse on the HF diet at 48 weeks (12.5%, n = 1/8), whereas no tumor was detected in WT mice ( n = 10).
Conclusions: Adiponectin may play a protective role in the progression of NASH in the early stages by suppressing tumor necrosis factor-α expression and liver fibrosis. 相似文献
Methods: Adiponectin KO mice and wild type (WT) mice were fed a HF diet or normal chow for the periods of 24 and 48 weeks. The HF diet contained 60% of calories from fat.
Results: The adiponectin KO mice on the HF diet showed obesity, marked elevation of serum transaminase levels, and hyperlipidemia. At 24 weeks, hepatic expression of tumor necrosis factor-α and procollagen α (I) was higher in KO mice as compared with WT mice. At 48 weeks, liver triglyceride contents in KO mice on normal chow were significantly higher than those in WT mice. Hepatocyte ballooning, spotty necrosis, and pericellular fibrosis around central veins were observed in KO mice on the HF diet. The pericellular fibrosis was more severe in KO mice on the HF diet than that in WT mice (1.62% vs 1.16%, P = 0.033). Liver adenoma and hyperplastic nodules developed in a KO mouse on the HF diet at 48 weeks (12.5%, n = 1/8), whereas no tumor was detected in WT mice ( n = 10).
Conclusions: Adiponectin may play a protective role in the progression of NASH in the early stages by suppressing tumor necrosis factor-α expression and liver fibrosis. 相似文献
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吡格列酮治疗大鼠非酒精性脂肪性肝病的研究 总被引:1,自引:0,他引:1
目的观察胰岛素抵抗与非酒精性脂肪性肝病的关系,探讨吡格列酮对高脂喂养诱导大鼠脂肪肝的治疗作用。方法32只雄性SD大鼠分为3组:对照组(11只)喂饲普通饮食16周,模型组(10只)喂饲高脂饮食16周,药物组(11只)为高脂饮食喂饲8周后改为高脂饮食同时加用吡格列酮4 mg·kg~(-1)·d~(-1)灌胃8周。16周末处死大鼠,称量肝湿重及体重,测定丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)和血糖水平。用放射免疫法测定空腹胰岛素和肿瘤坏死因子(TNF)-α,计算胰岛素抵抗指数(HOMA-IR),并观察肝脏病理变化。结果16周末,模型组肝脏出现明显肝细胞脂肪变性和肝小叶炎症,肝指数、ALT、AST、ALP、胰岛素水平及HOMA-IR均高于对照组(P<0.05);药物组和模型组相比,脂肪变性和肝小叶炎症明显减轻,肝指数、ALT、ALP、TNF-α和HOMA- IR均降低(P<0.05),胰岛素水平有降低趋势。结论高脂喂养脂肪肝大鼠随病变进展可能存在胰岛素抵抗。吡格列酮治疗可以改善或延缓大鼠脂肪肝病变程度。 相似文献
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[目的]探究苦龙胆酯苷(MAG)对高脂饮食(HFD)所致动脉粥样硬化的拮抗作用及其机制。[方法]将30只ApoE-/-小鼠随机分为对照组(正常饮食)、HFD组、HFD+MAG组,每组10只小鼠,其中HFD+MAG组每日予50 mg/kg MAG灌胃,持续12周。生物化学检测仪测定血脂水平;油红O染色观察主动脉斑块沉积情况;苏木精-伊红(HE)染色观察主动脉血管病理形态表现;Mac-3免疫组织化学染色观察主动脉巨噬细胞聚集情况;免疫荧光定位染色观察NOD样受体热蛋白结构域相关蛋白3(NLRP3)表达与分布;Western blot检测主动脉中NLRP3、凋亡相关斑点样蛋白(ASC)和Caspase-1亚基p20表达水平。[结果] HFD+MAG组血清甘油三酯、总胆固醇和低密度脂蛋白胆固醇水平分别降低至HFD组的88.39%、74.85%和64.97%(均P<0.05),高密度脂蛋白胆固醇水平升高至HFD组的142.18%(P<0.05),斑块面积减少至HFD组的45.16%(P<0.05),MAG能改善主动脉病理形态表现,使巨噬细胞沉积减少,斑块内... 相似文献
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马来酸罗格列酮干预高脂饲养大鼠脂肪肝的研究 总被引:2,自引:0,他引:2
目的观察高脂饲养所致正常SD大鼠脂肪肝与胰岛素抵抗的关系及罗格列酮干预脂肪肝的效果。方法将8周龄雄性SD大鼠随机分为三组,每组各11只:正常饲养组(NC)、高脂饲养组(HF)、高脂+罗格列酮组(HF4-Ros)。饲养8周后取空腹血测定血糖、脂联素,胰岛素敏感性用正常血糖高胰岛素钳夹术稳态时的葡萄糖输注率(GIR)来评价,测定肝脏中TG含量,并对大鼠肝脏组织做HE染色。结果高脂饲养8周后,与NC组相比,HF组肝脏呈现明显脂肪肝,肝脏中TG明显增加达154.2%(NC组3.89mg/g,HF组9.89mg/g,P〈0.01);与HF组相比,HF4-Ros组肝脏中TG显著降低达46.6%(5.28mg/g,P〈0.01),脂肪肝减轻。结论马来酸罗格列酮能明显改善高脂饲养大鼠的脂肪肝。 相似文献