扶正胶囊提取物对环磷酰胺诱导的免疫低下小鼠免疫调节作用研究

[目的] 探讨扶正胶囊提取物对免疫抑制小鼠免疫功能的调节作用。[方法] 将60只昆明小鼠进行随机分组,每组10只,分为空白组、模型组、阳性对照组以及扶正胶囊提取物低、中、高剂量组。采用环磷酰胺腹腔注射建立小鼠免疫功能低下模型,检测各组小鼠的胸腺指数和脾脏指数、单核细胞吞噬能力、血清中白细胞介素-4（IL-4）、白细胞介素10（IL-10）、白细胞介素-17（IL-17）、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）水平和脾T淋巴细胞亚群CD3⁺T淋巴细胞（CD3⁺）、CD4⁺T淋巴细胞（CD4⁺）、CD8⁺T淋巴细胞（CD8⁺）的分布与比例。[结果] 扶正胶囊提取物能提升由环磷酰胺诱导的免疫低下小鼠的胸腺指数和脾脏指数,增强单核细胞吞噬功能,显著提高小鼠细胞免疫因子IL-4、IL-10、IFN-γ含量,降低IL-17、TNF-α含量,上调脾淋巴细胞亚群CD3⁺CD4⁺的表达程度和提升CD3⁺CD4⁺/CD3⁺CD8⁺比值。[结论] 扶正胶囊提取物能够提升环磷酰胺所致的免疫抑制小鼠的免疫功能。     

儿童过敏性鼻炎治疗前后外周血CD4+CD25+CD127lo/-Treg细胞与炎症因子变化分析

目的 探讨儿童过敏性鼻炎（AR）治疗前后外周血CD4⁺CD25⁺CD127^lo/-Treg及白细胞介素2（IL-2）、肿瘤坏死因子α（TNF-α）、人转化生长因子β1（TGF-β1）的水平变化及临床意义。方法 将厦门长庚医院收治的108例AR患儿作为AR组,根据疾病严重程度分为轻度、中重度组,另采用单纯随机抽样法选取年龄、性别匹配的80例健康儿童作为对照组,检测两组对象入院时CD4⁺CD25⁺CD127^lo/-Treg、IL-2、TNF-α、TGF-β1水平;AR患者均给予对症治疗,检测治疗前、治疗1个月、治疗6个月CD4⁺CD25⁺CD127^lo/-Treg、IL-2、TNF-α、TGF-β1水平,采用Pearson相关分析疾病严重程度与外周血CD4⁺CD25⁺CD127^lo/-Treg的相关性及两者与IL-2、TNF-α、TGF-β1相关性。结果 AR患者CD4⁺CD25⁺CD127^lo/-Treg、IL-2、TGF-β1水平分别为（6.264±0.135）%、（50.16±11.67）ng/L、（14.21±5.34）ng/L,均低于对照组;TNF-α为（1.82±0.62）ng/L,高于对照组的（0.34±0.19）ng/L,差异均有统计学意义（P<0.05）。轻度AR患者CD4⁺CD25⁺CD127^lo/-Treg、IL-2、TGF-β1水平分别为（6.305±0.137）%、（59.34±13.05）ng/L、（16.97±4.69）ng/L,均高于中重度AR组患者,TNF-α水平为（1.64±0.57）ng/L,低于中重度AR组患者的（1.93±0.65）ng/L,差异均有统计学意义（P<0.05）。AR组治疗后第1、6个月CD4⁺CD25⁺CD127^lo/-Treg、IL-2、TGF-β1水平高于治疗前,TNF-α水平低于治疗前,差异均有统计学意义（P<0.05）。Pearson相关分析显示：外周血CD4⁺CD25⁺CD127^lo/-Treg、TNF-α与疾病严重程度呈正相关关系（r=0.681、0.581,P<0.05）,IL-2、TGF-β1与疾病严重程度呈负相关关系（r=-0.613、-0.579,P<0.05）;外周血CD4⁺CD25⁺CD127^lo/-Treg与IL-2、TGF-β1呈负相关性（r=-0.675、-0.691,P<0.05）,与TNF-α呈正相关关系（r=0.618,P<0.05）。结论 AR患者存在CD4⁺CD25⁺CD127^lo/-Treg及IL-2、TGF-β1下降,TNF-α上升,且与病情程度有关,治疗后均明显改善。     

HIV/AIDS患者CD4+T淋巴细胞检测结果与抗病毒治疗效果

目的 探讨某地区HIV/AIDS患者CD4⁺T淋巴细胞检测结果与抗病毒治疗效果,同时进行相关性分析。方法 2013年2月~2016年1月选择在笔者医院诊治的HIV/AIDS患者60例作为观察组,同期选择在笔者医院进行健康体检的健康人60例作为对照组,观察组都给予HAART标准治疗方案,观察组在治疗前后、对照组在入院时都进行CD4⁺T淋巴细胞与细胞因子表达的检测。结果 观察组外周血的CD4⁺CD45RA⁺、CD4⁺CD28⁺、CD4⁺CD45RO⁺的细胞比例明显低于对照组（P<0.05）,两组CD4⁺CD25⁺的细胞比例对比,差异无统计学意义（P>0.05）。与对照组相比,观察组的血浆IL-10和TGF-β1含量都显著升高（P<0.05）。治疗后观察组的CD4⁺CD45RA⁺、CD4⁺CD28⁺、CD4⁺CD45RO⁺的细胞比例都明显上升（P<0.05）,而血浆IL-10和TGF-β1含量明显降低（P<0.05）。在观察组中,Spearman秩相关分析显示CD4⁺CD45RA⁺、CD4⁺CD28⁺、CD4⁺CD45RO⁺的细胞比例与IL-10、TGF-β1水平均呈负相关（P<0.05）。结论 HIV/AIDS患者只有较低的免疫功能,具有较低的CD4⁺T淋巴细胞亚群比例和较高的IL-10和TGF-β1水平,HAART治疗能改善免疫功能,为此通过CD4⁺T淋巴细胞就能判断HIV/AIDS患者的治疗效果和病情。     

三焦针法对阿尔茨海默病免疫功能影响的研究

[目的]观察三焦针法对阿尔茨海默病（AD）的临床疗效及对患者免疫功能的影响。[方法]收集AD患者32例和健康老年人30例。AD组给予三焦针法,取穴膻中、中脘、气海、血海、足三里和外关,健康组不给予任何干预。利用简易精神状态检查表（MMSE）、临床痴呆量表（CDR）、老年性痴呆评估量表-认知分量表（ADAS-cog）和日常生活活动量表（ADL）评价AD患者针刺前后的认知功能、非认知功能和日常生活能力。应用流式细胞术和酶联免疫吸附法（ELISA法）检测外周血CD3⁺、CD3⁺CD4⁺、CD3⁺CD8⁺T细胞数量、CD4⁺/CD8+比例、免疫球蛋白（IgG）、白介素（IL）-1β、IL-2、IL-6、干扰素-γ（IFN-γ）的水平。[结果]针刺后AD组MMSE评分升高,CDR、ADAS-cog、ADL评分降低（P<0.05）。与健康组比较,AD组CD3⁺、CD8⁺T细胞数量减少,CD4⁺T细胞数量增加（P<0.05）,针刺后CD3⁺T细胞数量增加（P<0.05）。与健康组比较,AD组IL-1β、IL-2、IL-6、IFN-γ水平增加（P<0.05）,IgG未见明显改变（P>0.05）,针刺后以上细胞因子水平降低（P<0.05）。[结论]三焦针法可能通过调节AD患者的免疫功能而改善其痴呆状态。     

幼儿贫血患者的调节T细胞表达及临床预后相关性研究

[目的]探讨调节性T细胞在幼儿贫血中的表达及临床预后价值。[方法]选择2016年3月-2017年3月天津市儿童医院治疗的贫血患儿80例作为研究对象,设为观察组;根据贫血严重程度分为轻度组、中度组与重度组;所有患儿均给予铁剂、维生素及饮食强化治疗。根据治疗预后分为预后良好组与预后差组;选择同期健康体检者56例,设为对照组。采用流式细胞仪完成各组调节性T细胞CD4⁺、CD4⁺CD125⁺、CD4⁺CD125⁺CD127^low水平;采用SPSS Pearson相关性分析软件对调节性T细胞与患儿治疗预后进行相关性分析。[结果]观察组调节性T细胞CD4⁺、CD4⁺CD125⁺、CD4⁺CD125⁺CD127^low百分比,均低于对照组（P<0.05）;观察组贫血患儿中预后良好组CD4⁺、CD4⁺CD125⁺、CD4⁺CD125⁺CD127^low百分比高于预后差组（P<0.05）。[结论]调节性T细胞在幼儿贫血中呈低表达,且与患儿临床预后呈负相关性,加强调节性T细胞测定能评估预后,指导临床治疗。     

白附子混悬液对H22荷瘤小鼠抗肿瘤作用的免疫调节机制研究

[目的]研究白附子混悬液对荷瘤小鼠免疫功能的调节作用,探讨其抗肿瘤作用的免疫学机制。[方法]采用H₂₂荷瘤小鼠模型为研究对象,用酶联免疫吸附实验(ELISA)法检测小鼠血清中白介素-2(IL-2)和白介素-4(IL-4)水平;用流式细胞术(FACS)法分析对CD₄⁺,CD₈⁺T细胞的调节作用。[结果]白附子混悬液能够上调荷瘤小鼠血清IL-2细胞因子的表达,降低IL-4水平,可以使CD₄⁺T细胞表达升高,CD₈⁺T细胞表达降低。[结论]白附子混悬液能够通过调节荷瘤机体异常免疫功能状态而发挥抗肿瘤作用。     

卡泊三醇治疗寻常性银屑病的临床疗效观察

目的 探讨卡泊三醇治疗寻常性银屑病的临床疗效。方法 选取2013年1月~2016年6月于中国人民解放军第一八四医院皮肤科收治的306例稳定期寻常性银屑病,随机分成对照组与试验组,每组153例。均予消银胶囊、维生素C、叶酸以及窄谱中波紫外线等基础治疗,对照组再予复方丙酸氯倍他索软膏外敷,试验组在上述基础上再予卡泊三醇软膏外敷。检测血清炎性细胞因子、T淋巴细胞亚群及皮肤屏障功能指标,评估皮损严重程度（PASI）与皮肤病学生活质量指数（DLQI）,比较治疗效果。结果 与治疗前比较,两组干扰素γ（IFN-γ）、白介素-2（IL-2）、白介素-17（IL-17）及白介素-18（IL-18）降低（P < 0.01）,CD3⁺、CD4⁺及CD4⁺/CD8⁺升高（P < 0.01）,CD8⁺降低（P < 0.01）,皮脂含量、角质层含水量升高（P < 0.01）,pH值降低（P < 0.01）,PASI评分、DLQI评分降低（P < 0.01）;与对照组比较,试验组IFN-γ、IL-2、IL-17及IL-18较低（P < 0.01）,CD3⁺、CD4⁺及CD4⁺/CD8⁺较高（P < 0.01）,CD8⁺较低（P < 0.01）,皮脂含量、角质层含水量较高（P < 0.01）,pH值较低（P < 0.01）,PASI评分、DLQI评分较低（P < 0.01）,治疗有效率较高（P < 0.01）。结论 卡泊三醇治疗寻常性银屑病效果显著,值得临床推广。     

异基因造血干细胞移植后小鼠共刺激分子表达与急性移植物抗宿主病的关系

目的 探究异基因造血干细胞移植后小鼠共刺激分子表达及其与急性移植物抗宿主病（aGVHD）发病的关系。方法 供体为5只C57BL/6J（H2KD^-H2KB⁺）雄性小鼠,受体为30只CB6F1（H2KD⁺H2KB⁺）雌性小鼠。将受体小鼠随机分为单纯照射组、骨髓移植组和aGVHD组,每组10只。单纯照射组：受体小鼠接受γ射线照射后不注射任何细胞;骨髓移植组：受体小鼠接受γ射线照射后注射5×10⁶个供体来源去T淋巴细胞的骨髓有核细胞;aGVHD组：受体小鼠接受γ射线照射后,同时注射5×10⁶个供体来源去T淋巴细胞的骨髓有核细胞和3×10⁷个供体来源脾细胞。采用log-rank生存曲线分析各组小鼠移植后生存情况。分别在移植后7、14、21、28 d采用流式细胞术检测骨髓移植组和aGVHD组小鼠外周血CD4⁺T淋巴细胞和CD8⁺T淋巴细胞表面共刺激分子细胞毒性T淋巴细胞相关抗原4（CTLA-4）、程序性死亡蛋白1（PD-1）、可诱导共刺激分子（ICOS）和CD28的表达情况,以及移植后21 d两组小鼠脾组织CD4⁺T淋巴细胞内白细胞介素（IL）-17、γ干扰素、IL-4的表达情况,并在移植后21 d采用3,3''-二氨基联苯胺（DAB）染色检测两组小鼠结肠组织共刺激分子配体的表达情况。结果 单纯照射组受体小鼠均在γ射线照射后19 d内死亡;骨髓移植组小鼠在γ射线照射后均未出现aGVHD症状,30 d内均存活;aGVHD组小鼠在γ射线照射后出现aGVHD症状的时间为14（11~18）d,生存时间为22（13~30）d。随着时间的推移,aGVHD组小鼠外周血CD4⁺和CD8⁺T淋巴细胞表面CTLA-4、PD-1表达在移植后均呈下降趋势,ICOS、CD28表达均呈上升趋势。在移植后28 d时aGVHD组小鼠外周血CD4⁺和CD8⁺T淋巴细胞表面CTLA-4、PD-1表达均低于骨髓移植组,ICOS、CD28表达均高于骨髓移植组,差异均有统计学意义（P均<0.05）。DAB染色结果示,骨髓移植组结肠组织CD80、ICOS配体（ICOSL）、PD-1配体（PD-L1）表达均为阴性,aGVHD组结肠组织CD80、ICOSL、PD-L1阳性表达率分别为40%、80%、80%。与骨髓移植组比较,aGVHD组小鼠脾组织CD4⁺T淋巴细胞内IL-17、γ干扰素表达均增加,IL-4表达减少,差异均有统计学意义（P均<0.05）。结论 异基因造血干细胞移植小鼠发生aGVHD后,共刺激分子CTLA-4表达随时间推移逐渐降低。CTLA-4、ICOS、PD-1均可能通过调节辅助性T细胞（Th）1、Th2、Th17等T淋巴细胞亚群分布参与aGVHD的发生、发展。     

胸腺五肽对新辅助化疗的乳腺癌患者局部组织中浸润淋巴细胞的影响

目的 探讨胸腺五肽对新辅助化疗的乳腺癌组织微环境中浸润淋巴细胞的影响。方法 自2012年1月~2014年1月,前瞻性收集笔者医院收治的接受新辅助化疗的乳腺癌患者80例,将患者随机分为研究组和对照组,所有患者均行新辅助化疗,研究组在新辅助化疗期间给予胸腺五肽,化疗结束后进行手术切除肿瘤组织,留取乳腺癌组织标本,观察乳腺癌组织中和外周血中CD4⁺T细胞、CD8⁺T细胞、CD4⁺/CD8⁺T细胞比例、外周血中IL-6、IL-10、TGF-β1水平。结果 与对照组比较,研究组患者乳腺癌组织中CD4⁺T细胞显著降低（21.47%±3.29% vs 23.88%±3.43%,P=0.002）;CD8⁺T细胞显著增高（30.36%±4.38% vs 28.43%±4.16%,P=0.047）;CD4⁺/CD8⁺T细胞显著降低（0.71±0.14 vs 0.84±0.18,P=0.001）。两组患者治疗前外周血CD4⁺T细胞、CD4⁺/CD8⁺T细胞、IL-6、IL-10、TGF-β1等比较,差异均无统计学意义（P>0.05）。与对照组比较,研究组患者术后外周血CD4⁺T细胞水平显著增加（27.67%±4.24% vs 25.09%±3.68%,P=0.005）;CD4⁺/CD8⁺T显著增加（0.83±0.14 vs 0.75±0.18,P=0.029）;IL-6水平显著增高（12.39±2.87 vs 9.24±3.38ng/L,P=0.000）;IL-10水平显著降低（8.49±2.59 vs 12.59±3.65ng/L,P=0.006）;TGF-β1水平显著降低（12.47±3.88 vs 17.76±4.12ng/ml,P=0.000）。两组患者住院时间、切口感染、手术部位出血、复发率和病死率等比较,差异均无统计学意义（P > 0.05）。结论 胸腺五肽联合新辅助化疗可能有助于改善乳腺癌组织微环境中免疫状态和全身免疫功能。     

结核分枝杆菌热休克蛋白65对ApoE基因敲除小鼠Treg/Th17免疫平衡的影响

研究结核分枝杆菌热休克蛋白65(HSP65)对ApoE^-/-小鼠Treg/Th17免疫平衡的影响。首先,将结核分枝杆菌HSP65蛋白免疫高脂饲喂ApoE^-/-小鼠,再采用ELISA检测小鼠血清中抗HSP65抗体的产生情况,流式细胞术检测血中CD4⁺CD25⁺Foxp3⁺调节性T细胞(Treg)和CD4⁺IL-17⁺辅助性T细胞17(Th17)的数量,ELISA检测血清中IL-10、TGF-β1、IL-17和IL-21含量,生化分析仪检测血中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)的含量,染色法计算动脉粥样硬化斑块面积。结果发现结核分枝杆菌HSP65蛋白诱导ApoE^-/-小鼠产生了高水平的抗HSP65抗体,Treg细胞及其分泌的IL-10和TGF-β1细胞因子数量明显减少,Th17细胞及其分泌的IL-17和IL-21细胞因子数量明显增加,TC、TG、HDL-C及LDL-C的含量未见改变,但动脉粥样硬化斑块的面积明显增加。由此可见结核分枝杆菌HSP65蛋白能够破坏ApoE^-/-小鼠Treg/Th17免疫平衡,促进动脉粥样硬化的发展。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.