消化道肿瘤患者红细胞促淋巴细胞增殖反应的检测及意义

目的 从红细胞促淋巴细胞增殖角度探讨消化道肿瘤患者红细胞免疫功能状况。方法 采用MTT(四甲基噻唑蓝)比色法检测健康人和消化道肿瘤病人红细胞(RBC)促淋巴细胞(PBL)增殖作用。结果 健康对照组RBC促PBL增殖作用(57.3％±10.7％)明显高于消化道肿瘤患者(30.1％±6.8％),P<0.01,而肝癌患者(29.3％±4.3％)与食道癌患者(32.6％±8.6％)之间也有显著性差异P<0.05。结论 检测消化道肿瘤患者RBC促PBL增殖能力,可为临床观察患者RBC免疫功能状况提供新的实验方法。     

消化道肿瘤患者红细胞促淋巴细胞增殖反应的检测及意义

目的从红细胞促淋巴细胞增殖角度探讨消化道肿瘤患红细胞免疫功能状况。方法采用MTT(四甲基噻唑蓝)比色法检测健康人和消化道肿瘤病人虹细胞(RBC)促淋巴细胞(PBL)增殖作用。结果健康对照组RBC促PBL增殖作用(57．3%±10．7％)明显高于消化道肿瘤患(30．1％±6．8％)，p＜0.01，而肝癌患(29．3%士4．3％)与食道癌患(326%士8．6%)之间也有显性差异P＜O．05。结论检测：肖化道肿瘤患RBC促PBL增殖能力，可为临床观察患RRC免疫功能状况提供新的实验方法。     

消化系统肿瘤患者红细胞促淋巴细胞增殖反应的影响

我们采用四甲基偶氮唑蓝（ＭＴＴ）比色法检测消化系统肿瘤患者红细胞（ＲＢＣ）促淋巴细胞（ＰＢＬ）的增殖反应,以探讨消化系统肿瘤虱红细胞的免疫调控功能状况。结果发现：健康人ＲＢＣ促ＰＢＬ增殖反应作用明显,而消化系统肿瘤患者ＲＢＣ 促ＰＢＬ增殖反应较健康人明显低下,但手术后有一定的改善,不同部位的肿瘤ＲＢＣ促ＰＢＬ增殖反应亦不相同,肝癌与食道癌有显著性差异。     

西宁地区正常人的红细胞免疫功能研究

目的:了解掌握西宁地区正常人红细胞免疫功能的正常值。方法:按文献[1]采用郭峰建立的最常用的三种检测指标和方法。结果:西宁地区健康人三种红细胞花环率(%):补体C3b受体花环率(R-C3bR)、红细胞免疫复合物IC花环率(R-ICR)、红细胞免疫粘附肿瘤细胞花环率(R-TRR)分别为13.47±3.05,3.61±1.46,31.32±6.06。结论:西宁地区正常人三种花环率正常值与外省各地区的正常值有显著差别,数值较外地低下。三种花环率与性别之间无显著性差别。     

红细胞CD35在抗原激活淋巴细胞免疫反应中的作用

目的探讨红细胞CD35在抗原激活淋巴细胞免疫反应中的作用。方法用淋巴细胞分离液分离健康成人ACD抗凝全血获得淋巴细胞悬液(1×106/ml)和红细胞悬液(1×108/ml),以灭活腹水癌细胞S180(1×106/ml)作为激活抗原,以自体血浆作为反应基质,考察红细胞对淋巴细胞的调控能力,流式细胞仪检测淋巴细胞表面CD25的表达阳性率。单抗封闭红细胞CD35或EDTA破坏补体,观察淋巴细胞CD25表达的变化。结果CD35单抗封闭后淋巴细胞CD25表达量(18·22%±4·27%)明显降低;EDTA破坏补体后,淋巴细胞CD25表达量(18·79%±3·90%)比ACD抗凝组(23·29%±4·40%)明显降低,与CD35阻断组基本相同,但仍高于Hanks′液对照组(13·19%±2·96%)。结论红细胞CD35和血浆补体对抗原激活淋巴细胞免疫反应起着重要的调控作用;血浆中还存在其他调控淋巴细胞活化的机制。促进红细胞表面CD35分子表达、保持血浆补体活性对提高淋巴细胞免疫活性有着重要意义。     

脐血红细胞CD35调控白细胞CXCR4表达能力的变化

目的探讨脐血红细胞调控白细胞CXCR4表达能力的变化。方法采用灭活癌细胞(S180,5×106/ml)0·2ml(或生理盐水)加入全血细胞(或白细胞)0·2ml和血浆0·3ml中,37℃温育1h,用流式细胞仪免疫荧光法测定红细胞CD35和白细胞(淋巴细胞、粒细胞)CXCR4分子表达量。分组:①癌细胞悬液0·2ml加全血细胞悬液0·2ml和血浆0·3ml;②生理盐水0·2ml加全血细胞悬液0·2ml和血浆0·3ml;③癌细胞悬液0·2ml加白细胞悬液0·2ml和血浆0·3ml;④生理盐水0·2ml加白细胞悬液0·2ml和血浆0·3ml。结果脐血红细胞CD35表达量在S180激活后下降不如正常人红细胞明显,而白细胞CXCR4表达量在S180激活后上升不明显;正常人全血红细胞CD35表达量在S180激活后明显下降(40·21%±11·52%vs28·65%±8·08%,P<0·01),而粒细胞CXCR4表达量明显上升(40·62%±17·89%vs64·18%±11·69%,P<0·01)。结论脐血红细胞免疫调控能力和白细胞免疫功能都明显低下。     

肺癌患者红细胞免疫功能与外周血T淋巴细胞亚群的改变及其相关因素研究

 目的 为了观察肺癌患者红细胞免疫功能及T淋巴细胞亚群的变化.方法 采用检测55例肺癌患者红细胞免疫功能及T淋巴细胞亚群,并与15例肺良性肿瘤患者和20例正常人对照.结果 (1)肺癌组红细胞C3 b受体花环率(RBC-C 3 bRR)、CD 3<'+>、CD4<'+>、CD 8<'+>、CD4<'+>/CD8<'+>比值低于正常人(P<0.05～0.01),红细胞免疫复合物花环率(RBC-ICR)依次高于肺良性肿瘤组、正常人(P<0.05～0.01).(2)肺癌RBC-C 3 bRR与CD4<'+>/CD 8<'+>成直线正相关(P<0.01,r=0.9131).(3)Ⅲa、Ⅲb期肺癌,RBC-C 3 bRR、CD 3<'+>、CD4<'+>、CD 4<'+>/CD 8<'+>比值低于Ⅰ、Ⅱ期肺癌患者、正常人(P<0.05～0.01),而RBC-ICR高于正常人(P<0.01).Ⅰ、Ⅱ期病人CD3<'+>、CD 4<'+>、CD 8<'+>低于正常人(P<0.01).RBC-ICR高于正常人(P<0.01).(4)术后10～14 d,根治组与术前比较:RBC-C 3 bRR、CD 3<'+>、CD 4<'+>/CD 8<'+>比值显著升高(P<0.01),而RBC-ICR、CD 8<'+>显著降低(P<0.01);术后与正常人比较:RBC-C 3 bRR、CD4<'+>/CD 8<'+>比值无显著差异(P>0.05).姑息组与术前比较:RBC-C 3 bRR、CD 4<'+>、CD 4<'+>/CD 8<'+>比值显著升高(P<0.05～0.01),而RBC-ICR显著下降(P<0.01),CD 3<'+>、CD 8<'+>无明显改变,术后与正常人比较:RBC-C 3 bRR、CD4<'+>/CD 8<'+>比值无显著差异(P>0.05).探查组与术前比较:两者指标无显著差异(P>0.05).结论 肺癌患者RBC免疫和T细胞亚群的检测及其相关性分析,对肺癌的诊断,治疗及病情预后估计有一定价值.     

肺癌患者外周血淋巴细胞CD44分子的表达

 目的探讨外周血淋巴细胞中黏附分子CD44在肺癌患者中的表达及其临床意义.方法用流式细胞仪定量检测72例肺癌患者外周静脉血中CD44水平,与正常人及慢性支气管炎患者进行对照,应用统计学分析比较.结果肺癌组外周血CD44含量为(57.85±1.99),明显高于正常人组(43.03±2.33)(P＜0.01)及慢性支气管炎组(45.37±1.79)(P＜0.01),CD44的表达水平与患者病理类型不呈相关性(P＜0.05),但与临床分期和组织学分级相关(P＜0.01或P＜0.05).结论外周血淋巴细胞中CD44含量可作为肺癌患者诊断的参考指标.     

原发性肝癌患者红细胞调控IL-8新的实验体系研究

目的采用血液免疫反应路线图实验体系探讨原发性肝癌患者红细胞调控白细胞分泌IL-8的能力。方法将癌细胞(S180)悬液0·2ml(5×106/ml)或生理盐水0·2ml加至枸橼酸抗凝全血细胞悬液(或白细胞悬液)0·2ml和血浆0·3ml中,37℃水浴1h,酶联免疫法测定反应液中IL-8含量。结果原发性肝癌患者全血细胞实验组、全血细胞对照组、白细胞实验组和白细胞对照组的IL-8含量(pg/ml)分别为376·35±243·96、353·64±271·92、461·27±277·11和424·97±278·93,正常人相应各组则分别为11·36±6·93、4·98±4·35、29·41±30·66和20·77±24·20;原发性肝癌患者全血细胞实验组、白细胞实验组和正常人全血细胞实验组、白细胞实验组的癌细胞激活率(%)分别为0·22±0·24、0·25±0·53、2·49±2·33和0·75±0·21;原发性肝癌患者全血细胞实验组、全血细胞对照组和正常人全血细胞实验组、全血细胞对照组的红细胞IL-8吸附率(%)分别为0·22±0·18、0·17±0·33、1·18±2·29和0·86±0·49。原发性肝癌患者红细胞IL-8激活率及IL-8吸附率均明显低于正常人(P<0·01)。结论采用血液免疫反应路线图实验体系测定红细胞对IL-8的调控作用方法简便、实用。原发性肝癌患者红细胞对IL-8含量的调控能力明显下降。     

高原地区进展期胃癌患者红细胞免疫功能和T淋巴细胞亚群的研究

目的：研究高原地区进展期胃癌（AGC）患者的红细胞免疫功能和淋巴细胞免疫功能;方法：对我科住院手术治疗的32例进展期胃癌患者作了红细胞C3b受体花环（RBC－C3bRR)、红细胞免疫复合物花环（RBC－ICR）及淋巴细胞亚群CD3、CD4、CD8、CD4／CD8的检测,并与20例正常人结果进行比较;结果：高原地区进展期胃癌患者RBC－C3bRR,CD3,CD4,CD8、CD4／CD8比值均低于正常人（P＜0．01）,RBCICR高于正常人（P＜0．01）;结论：高原地区进展期胃癌患者红细胞免疫系统与淋巴细胞免疫系统均处于抑制状态,2个免疫系统的改变有着密切的关系,对高原地区进展期癌患者应给予免疫支持。     

Immunohistochemical detection of an AB0-incompatible blood transfusion in formalin-fixed tissue

A fatal incident of an AB0 incompatible erythrocyte transfusion in a 75-year-old male patient who suffered from dilated cardiomyopathy with cardiac failure is reported. Blood group A red cells were transfused to the unintended recipient who had blood group 0. The patient died 45 min after the incompatible erythrocyte transfusion. The way the incident happened remained unclear and the immunohistochemical detection of AB0 incompatible erythrocytes in formaldehyde-fixed paraffin-embedded kidney, lung, liver and spleen tissue provided the only material evidence of the transfusion error. Received: 6 July 2000 / Accepted: 4 September 2000     

Ambulatory arterial blood pressure monitoring in patients before and after thyroidectomy

BACKGROUND/AIM: [corrected] Increased values of thyroid hormones in the clinical syndrome of hyperthyreosis affect blood pres sure values and its circadial variation. The aim of this study was to define the influence of hyperthyreosis on the values and circadial variations of arterial blood pressure, as well as to investigate the effect of thyroid surgery on blood pressure values. METHODS: We compared the 24-hour averages of systolic and diastolic blood pressure, their variations and their reduction during the night between 20 female patients with hyperthyroidism and hypertension de novo and 20 healthy females. We compared the values of 24-hour ambulatory monitoring performed before the surgery with the values gathered two weeks after the surgery. RESULTS: The 24-hour average systolic and diastolic blood pressure values were higher in the patients with hyperthyroidism than in the control group (p < 0.001). In the group of patients, the variations in blood pressure were significantly higher than they were in the group of healthy people (p < 0.001). The amplitude of the nocturnal reduction of blood pressure was also significantly lower in the patients with hyperthyroid status and hypertension, in comparison to the healthy persons (p < 0.001). Two weeks after the surgery, a significant reduction of blood pressure values (both for systolic and diastolic) appeared. CONCLUSION: The patients with hyperthyroidism caused hypertension had higher systolic and dia stolic blood pressure, higher variations in blood pressure and lower nocturnal reduction of blood pressure than healthy subjects. Thyroid surgery, as a control of thyroid function, optimised blood pressure very rapidly.     

分子检测非小细胞肺癌外周血中MUC1表达的临床意义

 目的探讨非小细胞肺癌(NSCLC)患者外周血微转移的分子诊断的临床意义.方法应用逆转录聚合酶链反应(RT-PCR)技术,对31例肺癌患者和10例肺良性病变患者外周血中MUC1基因mRNA表达进行检测.结果术前10例NSCLC患者检测到外周血肺癌微转移,外周血微转移与肺癌组织学类型,细胞分化程度及P-TNM分期均存在密切关系(P＜0.05).10例肺良性病变患者的外周血中未检测到MUC1 mRNA表达.结论MUC1基因mRNA RT-PCR法检测肺癌患者外周血微转移具有较高的特异性和敏感性,可为制定治疗方案和评估预后提供重要参考依据.     

非小细胞肺癌术后外周血循环肿瘤细胞的检测及其意义

 目的探讨对非小细胞肺癌术后外周血中循环肿瘤细胞(circulatingtumorcells,CTCs)的检测及意义。方法经根治手术治疗并经病理确诊的肺腺癌患者38例,术后TNM分期为Ⅰ期19例,Ⅱ期6例,Ⅲ期13例。10例肺良性病变患者作为对照组,术后病理为肺结核4例,肺部炎性病变6例。采用免疫磁珠富集联合流式细胞术方法检测外周血CTCs(每7.5ml)。结果10例肺良性病变患者术后外周血中CTCs阳性率为10%(1/10),38例非小细胞肺癌患者术后外周血中CTCs阳性率为39.5%(15/38)。肺良、恶性组CTCs阳性率比较差异无统计学意义(P=0.132)。Ⅰ期与Ⅲ期CTCs阳性率相比差异具有统计学意义(χ²=3.970,P=0.046)。结论非小细胞肺癌术后外周血中存在CTCs,其阳性率与肿瘤TNM分期相关。CTCs检测可用于指导术后治疗。     

成份输血对急性颅脑创伤患者围术期脑氧供需平衡的影响

 目的研究红细胞输注对急性颅脑创伤患者围术期脑氧供需平衡的影响.方法随机选择40例急性闭合性颅脑损伤行开颅探查患者,对照组20例,输入全血;试验组20例,输入红细胞.监测患者围术期颈静脉球及桡动脉血气,评价其对脑氧供需平衡的影响.结果红细胞输入组术毕脑氧摄取率(CEO2)和动脉与颈静脉球氧含量差[C(a-jv)]O2与对照组相比无明显变化(P＞0.05).结论在维持血流动力学稳定状况下,单纯红细胞输入不影响急性颅脑损伤患者围术期脑氧供需平衡.     

早期肺癌外周血中肿瘤细胞的检测及其临床意义探讨

目的探讨检测早期肺癌循环肿瘤细胞(CTCs)的可行性及临床意义。方法应用免疫磁性分离(IMS)＋免疫细胞化学(ICC)法及RT-PCR法检测3组标本(A组：早期肺癌组,15例;B组：肺部良性病变组,20例;C组：健康志愿者组,20例)的CTCs,并对A组患者进行了6～9个月的随访。结果应用IMS＋ICC法检测,A组有5例阳性,B、C组均为阴性;应用巢式RT-PCR法检测,A组7例、B组8例、C组6例检测为阳性。A组患者随访中有2例术前及术后检测均为阳性的患者发生了复发。结论IMS＋ICC法是检测早期肺癌CTCs的敏感方法;早期肺癌患者CTCs的检出可能与临床预后有一定的关系。     

MSCT支气管动脉三维成像对中心型肺癌的血供研究

目的：探讨多层螺旋CT(MSCT)三维支气管动脉成像在中心型肺癌中的影像学表现．为显示中心型肺癌的血供来源及介入治疗提供理论依据。方法：对18例中心型肺癌的患者行MSCT造影增强检查，采用实时螺旋薄层CT扫描，在独立工作站行支气管动脉三维重建，使用不同的旋转轴观察支气管动脉的空间解剖细节。结果：其中6例(33．33％)中心型肺癌的支气管动脉三维图像能够清晰显示出支气管动脉的起源、走行的路径(肺内段和纵隔段)和血管直径等。有肺动脉明显狭窄截断的病例支气管动脉显示率最高，且明显增粗，表明此类肺癌的血供来源主要以支气管动脉为主。三维成像中以容积再现(VR)技术显示最佳。结论：MscT三维支气管动脉成像能够准确、直观地显示支气管动脉的空间解剖特点，为中心型肺癌的血供研究及介入治疗提供理论基础。     

Bcl-2 and Bax protein interaction in B-lymphocytes of peripheral blood in patients with chronic lymphocytic leukemia

BACKGROUND: Chronic lymphocytic leukemia (CLL) is a neoplastic disease characterized by the accumulation of morphologically mature monoclonal CD5+ B cells in the early phase (G0/G1) of the cell cycle. The accumulation of neoplastically transformed B-lymphocytes (CLL cells) is primarily the consequence of apoptosis blocking in these cells. Bcl-2 proteins are well-known modulators of this process. Some of these proteins are anti-apoptotic while the others are pro-apoptotic. All contain at least one of the four conserved regions called the Bcl-2 homologous domains (BH1-BH4). Evidence indicates that Bcl-2 and Bax form homo- and heterodimers. The anti-apoptotic effect of Bcl-2 protein is based on its ability to bind Bax protein in the heterodimer form, and thus to block the forming of Bax/Bax proapoptotic homodimers. The ratio of Bcl-2/Bax represents the cell autonomous rheostat which determinates the type of the cell reaction to an apoptotic stimulus. METHODS: The aim of this study was to determine the level of interaction between these two proteins in CLL cells using the co-immunoprecipition method. The study included the analysis of 20 peripheral blood specimens from 20 patients with CLL, and 20 peripheral blood specimens from healthy persons, who were in the control group. Specimens were precipitated with the monoclonal antibody for Bcl-2 protein, and immunoblotted with the palyclonal antibady for Bax protein (IP: Bcl-2/WB:Bax). At the same time, specimens were precipitated with the polyclonal antibody for Bax protein, and immunoblotted with the monoclonal antibody for Bcl-2 protein (IP: Bax/WB:Bcl-2). The intensity of Bcl-2 and Bax protein's binding compared to the control samples of the peripheral blood from healthy persons, was increased in CLL cells. RESULTS: IP: Bax/WB: Bcl-2 showed a high level of "free" Bcl-2 protein which was not bound in the heterodimer form to Bax protein. Simultaneously IP: Bcl-2/WB: Bax showed that a higher quantity of Bax protein was bound in the heterodimer form to Bcl-2 protein as opposed to the quantity of pro-apoptotic Bax protein potentialy bound in the homodimer form. CONCLUSION: Further studies involving larger groups of patients are necessary to explore the potential significance of the Bcl-2/Bax protein ratio as a prognositc parameter in the CLL treatment.     

亮氨酸氨基肽酶在肝病及癌症诊断中的应用

目的了解肝病患者及胃癌、肺癌、食道癌、胰腺癌患者血清中的亮氨酸氨基肽酶(LAP)活性变化,探讨其临床意义。方法采用酶速率法试剂检测血清LAP的活性,选择25例健康献血员做对照,对63例肝病患者和64例其它癌症患者血清LAP活性进行测定。结果在肝病组中肝癌LAP活性升高最大,与对照组和其他肝病组比较差异显著(P<005);在胃癌、肺癌、食道癌组中,LAP活性与对照组比较,无显著差异(P>005);但胰腺癌与对照组差异显著,13例中11例升高(P<005)。结论LAP活性在肝病中普遍升高,肝癌尤高,差异显著,为肝癌的鉴别诊断提供了依据;LAP活性在胰腺癌中的升高对其诊断也有一定临床意义。