外周血T淋巴细胞 Ag-NORs 与恶性肿瘤的关系

Ａｇ ＮＯＲｓ是与核仁组成区相关的嗜银蛋白的缩写。它对核糖体的形成及蛋白质的合成极为重要。当某种外因或内因所致血清中出现抑制性细胞因子时 ,Ａｇ ＮＯＲｓ含量降低 ,会不同程度地抑制免疫细胞的ｒＤＮＡ转录。因此 ,外周血Ｔ淋巴细胞核仁区酸性非组蛋白的转录活性是机体细胞免疫功能的重要标志。目前 ,许多学者[1～ 3] 将其引入肿瘤研究领域 ,通过观察外周血Ｔ淋巴细胞Ａｇ ＮＯＲｓ的数目、强度、位置和分布 ,可进行恶性肿瘤的筛选、诊断及病情监控。1　Ａｇ ＮＯＲｓ的主要生物学特征核仁组成区 (ｎｕｃｌａｒｏｒｇａｎｉｚｅｒ…     

GANGLIOSIDE GM_1 POTENTIATES NERVE GROWTH FACTOR EFFECTS ON REGENERATION OF CRUSHED SCIATIC NERVE IN RATS

ＧＡＮＧＬＩＯＳＩＤＥＧＭ１ＰＯＴＥＮＴＩＡＴＥＳＮＥＲＶＥＧＲＯＷＴＨＦＡＣＴＯＲＥＦ┐ＦＥＣＴＳＯＮＲＥＧＥＮＥＲＡＴＩＯＮＯＦＣＲＵＳＨＥＤＳＣＩＡＴＩＣＮＥＲＶＥＩＮＲＡＴＳＷＡＮＧＭｉｎ－ｓｈｅｎｇ（王民生），ＣＨＥＮＺｈｏｎｇ－ｗｅｉ（陈...     

Ag－NORs在胃粘膜良恶性病变的定量观察

Ａｇ－ＮＯＲｓ在胃粘膜良恶性病变的定量观察武警广西总队医院病理科陆爱权，刘时才，黎而介，黄振录（南宁５３０００３）核仁组成区（Ｎｕｃｌｅｏｌａｒｏｒｇａｎｉｚｅｒｒｅｇｉｏｎｓ）是出现在细胞核仁内与核糖体ＲＮＡ转录有关的ＤＮＡ环。自从１９８６年Ｐｌｏ...     

GROSS TRANSPLANTATION OF A LEG IN CASES OF MULTISEVERED LIMBS AND LITERATURE REVIEW

ＧＲＯＳＳＴＲＡＮＳＰＬＡＮＴＡＴＩＯＮＯＦＡＬＥＧＩＮＣＡＳＥＳＯＦＭＵＬＴＩ┐ＳＥＶＥＲＥＤＬＩＭＢＳＡＮＤＬＩＴＥＲＡＴＵＲＥＲＥＶＩＥＷＹＩＮＹｕｎ－ｓｈｅｎｇ（尹芸生），ＷＡＮＧＤｏｎｇ（王东），ＧＡＯＦｕ－ｇｕｉ（高富贵），ＬＵＺｈｉ（吕...     

Three-Dimensional Motion Measurement of the Atlantoaxial Joint

ＴＨＲＥＥ┐ＤＩＭＥＮＳＩＯＮＡＬＭＯＴＩＯＮＭＥＡＳＵＲＥＭＥＮＴＯＦＴＨＥＡＴ┐ＬＡＮＴＯＡＸＩＡＬＪＯＩＮＴＬＵＹｉ－ｓｈｅｎｇ（卢一生），ＪＩＡＬｉａｎ－ｓｈｕｎ（贾连顺），ＤＩＮＧＺｈｕ－ｑｕａｎ（丁祖泉），ＤＡＩＬｉ－ｙａｎｇ（戴力扬）Ｏ...     

EFFECT OF ENDOTOXIN ON ENDOTHELIN RECEPTOR AND EXPRESSIONO FENDOTHELIN-A RECEPTOR mRNA OF RAT LIVER IN ENDOTOXEMIA

ＥＦＦＥＣＴＯＦＥＮＤＯＴＯＸＩＮＯＮＥＮＤＯＴＨＥＬＩＮＲＥＣＥＰＴＯＲＡＮＤＥＸ┐ＰＲＥＳＳＩＯＮＯＦＥＮＤＯＴＨＥＬＩＮ┐ＡＲＥＣＥＰＴＯＲｍＲＮＡＯＦＲＡＴＬＩＶＥＲＩＮＥＮＤＯＴＯＸＥＭＩＡＬＩＵＢａｏ－ｈｕａ（刘宝华），ＣＨＥＮＨｕｉ－ｓ...     

酪氨酸磷酸酶抑制剂过矾酸钠对受照NFS—60细胞周期及细 …

目的：观察辐射对造血细胞的细胞周期及细胞凋亡的影响。蛋白质酪氨酸磷酸酶（ＰＴＰ）在造血细胞受体信号传递过程中，参与ＪＡＫ／ＳＴＡＴ通路及ＲＡＳ激活的负调控，与细胞增殖关系密切。过矾酸钠（ｓｏｄｉｕｍ ｐｅｒｖａｎａｄａｔｅ，ＰＥＲＶ）是ＰＴＰ特异性抑制剂，从而观察ＰＥＲＶ对受照ＮＦＳ－６０细胞周期及细胞凋亡的影响。方法：ＮＦＳ－６０细胞以适当数理接种于含１０％胎牛血清的ＲＰＭＩ１６４０培养基中，加     

A Comparison in Healing of Skull Defect Repaired with Four Different Kinds of Graft Materials in Rabbits

ＡＣＯＭＰＡＲＩＳＯＮＩＮＨＥＡＬＩＮＧＯＦＳＫＵＬＬＤＥＦＥＣＴＲＥＰＡＩＲＥＤＷＩＴＨＦＯＵＲＤＩＦＦＥＲＥＮＴＫＩＮＤＳＯＦＧＲＡＦＴＭＡＴＥＲＩＡＬＳＩＮＲＡＢ┐ＢＩＴＳＤＩＮＧＺｈｅｎ－ｑｉ（丁真奇）１，ＴＡＮＦｕ－ｓｈｅｎｇ（谭富生）２，...     

抗HBsAg人IgG表达载体的构建及其在CHO细胞中的表达

尝试将一株来源于人源性噬菌体抗体库的抗ＨＢｓＡｇ人Ｆａｂ,转换成完整的抗ＨＢｓＡｇ人ＩｇＧ。方法：采用重叠ＰＣＲ方法,在抗ＨＢｓＡｇ人Ｆａｂ的Ｖｋ和ＶＨ基因的５’端接上合工合成的人ｋ链的前导序列,构建表达完整ＩｇＧ１的真核表达载体,转染ＣＨＯ（ＤＨＦＲ＾－）细胞,用ＥＬＩＳＡ、ＲＴ－ＰＣＲ和免疫印迹检测抗ＨＢｓＡｇ人ＩｇＧ的表达,通过ＤＨＦＲ／ＭＴＸ体系及金属离子诱导提高Ｉｇ表达。结果：获得表达量     

OSTEOGENIC POTENTIAL OF RABBIT DERMAL FIBROBLASTS CULTURED IN VITRO: A HISTOCHEMICAL AND RADIOAUTO-GRAPHICAL STUDY

ＯＳＴＥＯＧＥＮＩＣＰＯＴＥＮＴＩＡＬＯＦＲＡＢＢＩＴＤＥＲＭＡＬＦＩＢＲＯＢＬＡＳＴＳＣＵＬＴＵＲＥＤＩＮＶＩＴＲＯ：ＡＨＩＳＴＯＣＨＥＭＩＣＡＬＡＮＤＲＡＤＩＯＡＵＴＯ┐ＧＲＡＰＨＩＣＡＬＳＴＵＤＹＣＨＡＩＢｅｎ－ｆｕ（柴本甫），ＴＡＮＧＸｕｅ－...     

Optimization of the system of medical service of students of Cadet Schools of the Ministry of Defense of the Russian Federation

One of the factors of the successful military career guidance Cadet schools students is preserving and promoting their health. Medical support of children and adolescents aged 10-17 years should include the full range of medical and preventive measures defined for this group. The state of providing outpatient care for pupils at the Cadet School in St. Petersburg was studied. These results show that full medical care in accordance with the standards can be based only on children's health clinics. It is important that the organization of medical support pupils cadet schools should be cooperate with civilian health care.     

带状疱疹83例误诊原因分析

带状疱疹是由水痘—带状疱疾病毒引起的皮肤科常见疾病。其主要的病理损害,一是受累神经的严重炎症性浸润,继而导致受侵犯神经节内神经细胞变性、坏死;二是皮肤的水泡。迅速抑制神经节和相应的感觉神经纤维的充血、水肿和坏死,防止粘连形成,达到迅速镇痛、改善皮损,缩短病程及防止后遗症的发生是治疗的关键。因而,尽早明确诊断,     

Estimation of accumulated dose of radiation by method of ESR-spectrometry of dental enamel of mammals

ESR-spectrometry was used to investigate radiation-induced paramagnetic centers in enamel of mammals: carnivores (polar bear and fox), ungulates (reindeer, European bison, moose), and man. Values at half the microwave power saturation of the radiation signal, P_1/2, evaluated at room temperature, was found to range from 16 to 26 mW for animals and man. A new approach to discrimination of the radiation induced signal from the total ESR spectrum of reindeer enamel is proposed. ‘Dose-response’ dependencies of enamel of different species mammals were measured within the dose range from 0.48 up to 10.08 Gy. Estimations of ‘radiosensitivity’ enamel of carnivores and ungulates showed good agreement with radiosensitivity enamel of man by ESR method.     

Analysis of the results of the international comparison of activity measurements of a solution of Fe

The results of an international comparison of activity measurements of a solution of ⁵⁵Fe organized by the BIPM in 2005 are reported and analysed. This exercise, which follows the procedures of the CIPM mutual recognition arrangement to update older comparisons, is a renewal of the comparison organized by the BIPM that took place in 1978. A EUROMET comparison was organized in 1996 specifically to compare activity measurements of a ⁵⁵Fe solution by means of liquid-scintillation techniques. Results of these three comparisons are presented and discussed in this paper.

The radionuclide solution was provided by the NPL, which also distributed the samples to the participants. The activity of the ampoules was measured by 16 laboratories using 12 methods producing 25 results. Some general considerations on uncertainty assessments pertaining to the different techniques used are drawn. The outcome of four different estimators is compared from which the presence of at least one outlier can be confirmed. Further measurements should be made to try to reduce the discrepancy between the results. To date the outcome of the present comparison does not show an improvement to that of the 1996 comparison.     

Evaluation of the results of various technics of non-surgical therapy of varicocele

A new method of non-surgical treatment of varicocele syndrome is described: it consists in sclerotherapy of spermatic vein by trans-femoral percutaneous catheterization with balloon-catheters. In 8 cases venous thrombosis has been induced by direct electric clotting. The techniques and a 6 months follow-up are discussed. It is pointed out that this procedure should be considered as the method of choice for tubular lesions and sub-fertility prophylaxis in young people and in childhood.     

延迟性脾破裂误漏诊原因分析

目的探讨延迟性脾破裂误漏诊原因和预防措施.方法回顾性分析总结12例延迟性脾破裂中的诊断和误漏诊的经验与教训.结果本组延迟性脾破裂的误漏诊5例(41.66%).对多发伤与脾破裂并存可能认识不足,外伤史轻微或伤员隐瞒外伤史,缺乏腹痛-缓解-突然再腹痛的典型病史,缺乏“对冲性脾破裂”力学分析和整体化诊断思路等为其误漏诊的主要原因.结论详细的外伤史和全面系统检查,重视腹以外多发伤掩盖腹内脏器伤及延迟性脾破裂可能.确立外伤-腹内脏器伤-脾破裂整体化诊断思路.不间断地辅以B超检查脾形态学变化和腹内有无积液,腹腔穿刺确定有无血腹、X线胸腹部检查观察左侧胸肋角和膈肌运动情况、必要时CT检查以尽早发现脾包膜下血肿,降低延迟性脾破裂误漏诊率.