彝族中老年人干眼症与正常眼结膜囊细菌状况对照研究

目的 比较彝族中老年人干眼症与正常人眼结膜囊细菌状况.方法 筛查出九龙县两个彝族聚居点的老年人群中干眼症70人140眼,刮取下睑结膜囊分泌物进行细菌培养分离鉴定.以同一人群中正常人66人132眼作为对照组进行分析对比.结果 干眼症组结膜囊细菌阳性率72.1％(101/140),正常组阳性率67.4％(89/132),差异无统计学意义(P=0.397).两组间不同性别之间差异无统计学意义(P＞0.05).干眼症95.2％(98/103)为革兰阳性菌,正常人群91.1％(82/90)为革兰阳性菌;优势菌种均为表皮葡萄球菌和棒状杆菌,其构成比差异无统计学意义(P＞0.05);干眼症组与正常组中单菌与多菌共存构成比、菌种平均数差异均无统计学意义(P＞0.05).结论 彝族老年人干眼症与正常人结膜囊细菌阳性率差别不明显;两组结膜囊细菌以革兰阳性菌为主;优势细菌种属及其构成比相似,均为结膜囊正常菌群.     

超重/肥胖患者高胰岛素血症与低钾周期性瘫痪的关系研究

【摘要】 目的比较彝族中老年人干眼症与正常人眼结膜囊细菌状况。方法筛查出九龙县两个彝族聚居点的老年人群中干眼症70人140眼,刮取下睑结膜囊分泌物进行细菌培养分离鉴定。以同一人群中正常人66人132眼作为对照组进行分析对比。结果干眼症组结膜囊细菌阳性率72.1%(101/140),正常组阳性率67.4%(89/132),差异无统计学意义(P=0.397)。两组间不同性别之间差异无统计学意义(P>0.05)。干眼症95.2%(98/103)为革兰阳性菌,正常人群91.1%(82/90)为革兰阳性菌;优势菌种均为表皮葡萄球菌和棒状杆菌,其构成比差异无统计学意义(P>0.05);干眼症组与正常组中单菌与多菌共存构成比、菌种平均数差异均无统计学意义(P>0.05)。结论彝族老年人干眼症与正常人结膜囊细菌阳性率差别不明显;两组结膜囊细菌以革兰阳性菌为主;优势细菌种属及其构成比相似,均为结膜囊正常菌群。 更多还原     

白内障超声乳化术前结膜囊细菌种类及药物敏感性

目的观察白内障超声乳化术前患者结膜囊细菌种类及药物敏感性。方法对白内障超声乳化术前398例398眼结膜囊取材进行细菌培养,并观察培养阳性的细菌对临床常见抗生素的药物敏感性。结果细菌培养阳性113眼,阳性率为28.4%。白内障患者术前结膜囊细菌主要由革兰阳性(G )菌构成(92.9%),其中G 球菌占87.6%,G 杆菌占5.3%。而革兰阴性(G-)杆菌占7.1%。G 球菌以表皮葡萄球菌为主(72.6%),其次微球菌占7.1%,金黄色葡萄球菌占6.2%。大多数菌株对氧氟沙星、妥布霉素、万古霉素、利福平及左氧氟沙星敏感性较好,耐药性<10%。结论表皮葡萄球菌是超声乳化术前患者结膜囊存在的最常见菌。术前预防性抗生素使用可选氧氟沙星、妥布霉素、利福平及左氧氟沙星。     

革兰阳性菌及革兰阴性菌所致新生儿社区获得性败血症晚发型临床分析

目的：探讨革兰阳性菌及革兰阴性菌所致新生儿社区获得性败血症晚发型的临床特点及药敏情况。方法：收集我院2009年1月至2012年12月收治的83例致病菌为革兰阳性菌和革兰阴性菌的新生儿社区获得性败血症晚发型的临床资料,进行回顾性分析。结果：83例新生儿社区获得性败血症晚发型中革兰阳性菌53例,占63．9％,以凝固酶阴性葡萄球菌、金黄色葡萄球菌为主;革兰阴性菌30例,占36．1％,以大肠埃希菌为主。检出的革兰阳性菌对红霉素、青霉素敏感率最低,对左氧氟沙星、庆大霉素、四环素部分敏感,万古霉素高度敏感。检出的革兰阴性菌对头孢噻吩、氨苄青霉素敏感率较低,对头孢他定、头孢曲松、环丙沙星、庆大霉素部分敏感,美罗培南高度敏感。革兰阳性菌感染多见于足月儿,革兰阴性菌感染多见于早产儿,对比两组围产情况无统计学差异,临床表现缺乏特异性。脐部感染多引起革兰阳性菌败血症。血小板降低多见于革兰阴性菌败血症,而在白细胞、杆状核细胞/中性柱细胞、CRP 上无差异。结论：新生儿社区获得性败血症晚发型临床表现无特异性,早期诊断需结合胎龄、感染部位、非特异性指标变化,抗生素应用应依据药敏结果。     

烧伤早期创面细菌学调查及药敏分析

目的:分析烧伤早期创面主要菌群和药物敏感性,指导烧伤临床抗生素的选择和应用。方法:对我科烧伤者(153例)创面分泌物进行菌株分离培养+药敏实验,并分析结果。结果:送检标本检出病原菌120株(78.4%),其中革兰阴性菌74株(61.7%。以铜绿假单胞菌和大肠埃希氏菌为主);革兰阳性菌46株(38.3%。以金黄色葡萄球菌和表皮葡萄球菌为主)。革兰阴性菌对妥布霉素、阿米卡星、庆大霉素、环丙沙星和左旋氧氟沙星较敏感,革兰阳性菌对万古霉素、呋喃妥因、替考拉宁和环丙沙星较敏感。部分病原菌呈多重耐药性。结论:早期烧伤创面仍以革兰阴性菌感染为主,及早进行创面细菌培养和药敏试验有利于合理选用抗生素。     

绵阳市中老年人结膜囊细菌状况调查分析

目的了解绵阳市中老年人结膜囊细菌状况。方法对绵阳市郊区两个聚居点的中老年人群160例320眼刮取下睑结膜囊分泌物,接种于血平板,培养48-72h后进行细菌分离鉴定。结果 320眼中结膜囊细菌培养阳性212眼,培养阳性率66.3%。培养出菌株238株;革兰阳性菌占94.1%;以棒状杆菌最多,共144株（60.5%）;表皮葡萄球菌53株（22.3%）。结论多数绵阳市汉族中老年人结膜囊带菌,以革兰阳性菌为主,棒状杆菌及表皮葡萄球菌为其结膜囊的优势菌种,尤以棒状杆菌为多。     

血液透析患者中心静脉留置导管相关性感染菌群分布及药敏分析

目的探讨血液透析患者中心静脉留置导管相关性感染菌群分布和药敏试验结果。方法选取2013年3月至2016年6月安阳地区医院38例经血液透析治疗的中心静脉留置导管相关性感染患者,采集导管血及外周血实施病原菌检查和药敏试验。结果38例患者共分离出43株细菌,其中革兰阳性菌占60.47%(26/43),革兰阴性菌占39.53%(17/43);表皮葡萄球菌对万古霉素、丁胺卡那敏感性较高,金色葡萄球菌对左氧氟沙星、头孢西丁、氯霉素、丁胺卡那、万古霉素敏感性较高,摩氏摩根菌及大肠埃希菌对亚胺培南、头孢哌酮舒巴坦敏感性较高,棒状杆菌对庆大霉素、万古霉素敏感性较高。结论血液透析患者中心静脉留置导管相关性感染主要致病菌包括表皮葡萄球菌、金色葡萄球菌,药敏结果可为干预方案的制定提供一定参考依据。     

羌族中老年人干眼症与正常眼结膜囊细菌状况对照研究

目的 比较羌族中老年人干眼症与正常人眼结膜囊细菌状况.方法 筛查出北川县两个羌族聚居点的老年人群中干眼症54人108眼,刮取下睑结膜囊分泌物进行细菌培养分离鉴定.以同一人群中正常人52人104眼作为对照组进行分析对比.结果 干眼症组结膜囊细菌阳性率57.4％(62/108),正常组阳性率61.5％(64/104)...     

灌注液中庆大霉素对白内障患者结膜囊细菌的作用

目的:分析白内障手术灌注液中庆大霉素对白内障患者结膜囊细菌的作用,为制定围手术期眼内炎预防措施提供依据。方法:采集200例白内障患者结膜囊拭子标本,进行细菌培养鉴定和药物敏感试验,观察检出细菌对20 μg/mL、40 μg/mL、80 μg/mL庆大霉素和2 μg/mL左氧氟沙星敏感率。结果:结膜囊细菌培养阳性检出率为62%,其中以表皮葡萄球菌为主,耐甲氧西林金黄色葡萄球菌（MRSA）检出率为3.22%,耐甲氧西林表皮葡萄球菌（MRSE）检出率为28.2%;全部检出细菌对20 μg/mL、40 μg/mL、80 μg/mL庆大霉素和2 μg/mL左氧氟沙星敏感率分别为79.84%、95.16%、97.58%、78.23%,中高浓度庆大霉素和左氧氟沙星比较,差异有统计学意义（均P<0.05）;MRSA和MRSE对20 μg/mL、40 μg/mL、80 μg/mL庆大霉素和2 μg/mL左氧氟沙星敏感率分别为69.23%、94.87%、100%、41.03%,各浓度庆大霉素和左氧氟沙星比较,差异有统计学意义（均P<0.05）。结论:白内障患者结膜囊细菌对灌注液中庆大霉素具有较高的敏感度,灌注液中加入适宜浓度庆大霉素,作为白内障围手术期眼内炎预防的措施应继续加以推广。     

儿童上呼吸道感染咽拭子标本细菌培养结果及耐药性分析

目的 探究某院儿童上呼吸道感染咽拭子标本细菌培养结果及耐药性。方法 将2019年8月～2022年9月河南黄河科技学院附属医院收治的上呼吸道感染患儿1053例纳入研究,所有患儿均进行咽拭子标本细菌培养与药敏试验,分析其培养结果及耐药性。结果 咽拭子标本涂片质量中,A级共125例,阳性率49.6%;B级共531例,阳性率16.01%;C级共305例,阳性率6.23%;D级92例,阳性率1.09%。1053例咽拭子标本检出阳性167例,检出率为15.86%(167/1053),其中革兰阳性菌检出121株,检出率为72.46%(121/167),革兰阴性菌检出46株,检出率为27.54%(46/167)。本次无真菌检出。革兰阳性菌中金黄色葡萄球菌检出率最高为88.43%,革兰阴性菌中大肠埃希菌检出率最高为43.48%。金黄色葡萄球菌对红霉素耐药性最高,为74.77%,对万古霉素、替考拉宁的敏感性均为100%;铜绿假单胞菌对氨苄西林耐药性最高,为100%,对环丙沙星、左氧氟沙星、美洛培南敏感性均为100%。大肠埃希菌对左氧氟沙星耐药性最高,为50%。病原菌耐药菌种的年度趋势分析中,以金黄色葡萄球...     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.