GM—CSF鉴别结核性和癌性胸膜炎的价值

采用放射免疫法检测２５例结核性和３１例癌性胸膜炎患者血清和胸水中粒细胞－巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）浓度。结果发现，结核组血清ＧＭ－ＣＳＦ［（１９．５６±１２．６４）ｎｇ／Ｌ］明显高于正常组［（９．５２±３．６１）ｎｇ／Ｌ，Ｐ〈０．０１］和癌性组［（９．３５±６．１３）ｎｇ／Ｌ，Ｐ〈０．０１］，而癌性组与正常组比较无显著性差异（Ｐ〉０．０５）；结核组胸水ＧＭ－ＣＳＦ浓度［（２４．３４±１４     

瘤体原位G—CSF基因治疗对化疗后结肠癌小鼠瘤体免疫功能？…

探讨瘤体原位脂质体介导的Ｇ－ＣＳＦ基因治疗抗肿瘤作用的机制。方法 将脂质体包裹的Ｇ－ＣＳＦ基因注射至结肠癌小鼠瘤体内，观察肿瘤浸润性淋巴细胞信全身免疫功能的变化。结果 治疗后，新鲜分离的ＴＩＬ的ＮＫ活性及经ＩＬ－２体外培养的ＴＩＬ杀伤Ｃ－２６细胞的活性均有明显提高；胸细胞与新鲜分离的Ｃ－２６细胞培养后诱导的ＣＴＬ活性以及外周血中性粒细胞杀伤Ｃ－２６细胞的活性显著高于对照组。     

烧伤延迟复苏与细胞集落刺激因子

为探讨严重烧伤延迟复苏血清粒细胞集落刺激因子（Ｇ－ＣＳＦ）的变化规律及其与感染发生，发展，预后的关系，采用大鼠３０％ＴＢＳＡⅢ度烧伤模型，动态了观察了大鼠在立即复苏与延迟复苏两种条件下，外周血白细胞数量，血清（Ｇ－ＣＳＦ）和肿瘤坏死因子－α（ＴＮＦ－α）含量，中性粒细胞吞噬功能；采用大鼠３０％ＴＢＳＡⅡ度烧伤延迟复苏合并早期创面感染模型，动态观察应用重组粒细胞－巨噬细胞集落刺激因子（ｒｈＧＭ－ＣＳ     

多发伤后血清细胞因子水平的动态观察

目的 检测多发伤后血清中各种细胞因子的水平,探讨其意义。方法 研究对象为多发伤创伤病人２０例。于伤１－７天,每日采空腹血样,用双抗体夹心酶联免疫法分别检测血清中肿瘤坏死因子,白细胞介素１β,６及粒细胞集落刺激因子,并进行了动态观察。结果 多发伤组ＴＮＦ－α,ＩＬ－２,ＩＬ－６明显高于对照组,而且在伤情严重的病人中ＴＮＦ－α和ＩＬ－２升高更为明显,Ｇ－ＣＳＦ的升高与严重感染有关。,     

p16与粒细胞-巨噬细胞集落刺激因子联合基因疗法治疗小鼠肾癌的研究

目的 探讨瘤体内直接注射途径的Ｐ１６抑癌基因疗法与粒细胞－巨噬细胞落刺激因子（ＧＭ－ＣＳＦ）的基因疗法联合应用对小鼠肾癌（ｒｅｎｅａ）的治疗效果及其免疫学机理。方法 建立皮下荷瘤肾癌小鼠模型,干接种肿瘤细胞３天后将荷瘤小鼠随机分为５组,分别给予不同的治疗,检测每组小鼠的免疫功能,观察每组小 的存活期,并行肿瘤病理学分析。结果 Ａｄｐ１６＋ＡｄＧＭ－ＣＳＦ联合治疗组有２只长期存活（存活时间〉９０ｄ）     

TK基因/9-丙氧鸟苷联合mGM-CSF基因治疗胃癌

目的 观察自杀基因ＴＫ对小鼠胃癌的杀伤作用,并联合ｍＧＭ－ＣＳＦ基因,观察免疫反应在增强ＴＫ杀伤性中的作用。方法 应用逆转录病毒方法转导自杀基因ＴＫ,应用脂质体方法转导细胞因子基因ｍＧＭ－ＣＳＦ。观察ＴＫ基因体外对小鼠胃癌６１５小鼠前胃癌（ＭＦＣ）细胞的杀伤性和旁观者效应;体内实验中将病毒上清注入瘤体内,并结合应用其前药９－丙氧鸟苷（ＧＣＶ）和ｍＧＭ－ＣＳＦ基因,分别观察肿瘤的生长情况。通过病理切     

c—erbB—2,c—erbB—4原癌基因蛋白表达与乳腺癌妆生的相关关系

目的 研究ｃ－ｅｒｂＢ－２、ｃ－ｅｒｂＢ－４原癌基因过度表达与乳腺癌发生及其病理参数的相关关系。方法 对８０例乳腺癌、１６例乳腺纤维瘤、９例乳腺纤维囊性病、１７例乳腺癌转移淋巴垄石蜡切块采用免疫组化（ＬＳＡＢ法）结合计算机图像分析研究原癌基因产物ｃ－ｅｒｂＢ－２、ｃ－ｅｒｂＢ－４的表达情况。结果 Ｃ－ｅｒｂＢ－２在乳腺癌中过度表达有显著意义。ｅ－ｅｒｂＢ－２在乳腺癌转移洒巴结中的表达较原发灶中高，     

Relationship between changes of N—methyl—D—aspartate receptor activity and brain after brain injury in rats

OBJECTIVE: To investigate the relationship between the changes of N-methyl-D aspartate (NMDA) receptor activity and brain edema after injury in rats. METHODS: The brain injury models were made by using a free-falling body. The treatment model was induced by means of injecting AP5 into lateral ventricle before brain injury; water contents in brain cortex were measured with dry-wet method; and NMDA receptor activity was detected with a radio ligand binding assay. RESULTS: The water contents began to increase at 30 minutes and reached the peak at 6 hours after brain injury. The maximal binding (B(max)) of NMDA receptor increased significantly at 15 minutes and reached the peak at 30 minutes, then decreased gradually and had the lowest value 6 hours after brain injury. Followed the treatment with AP5, NMDA receptor activity in the injured brain showed a normal value; and the water contents were lower than that of AP5-free injury group 24 hours after brain injury. CONCLUSIONS: It suggests that excessive activation of NMDA receptor may be one of the most important factors to induce the secondary cerebral impairments, and AP5 may protect the brain from edema after brain injury.     

联合应用c—Ha—ras,c—myc反义寡聚脱氧核苷酸对人膀胱癌细胞生长增殖…

作者联合应用人工合成的ｃ－Ｈａ－ｒａｓ，ｃ－ｍｙｃ反义寡聚脱氧核苷酸（ＡＳＯ－ｒ、ＡＳＯ－ｍ）对人膀胱癌细胞株进行作用，观察到ＡＳＯ－ｒ、ＡＳＯ－ｍ能明显抑制癌细胞中ｒａｓＰ２１、ｍｙｃＰ６２的表达，抑制了癌细胞ＤＮＡ合成及生长增殖，经ＡＳＯ－ｒ、ＡＳＯ－ｍ处理后癌细胞裸鼠致瘤力下降，这一结果表明：针对多外癌基因联合应有反义寡聚脱氧核苷酸，可能给膀胱癌的治疗提供新的途径，有进一步探索的价值。     

Eeffects of nerve growth factor on N—methyl—D—asparate receptor 1 after spinal cord injury in rats

OBJECTIVE: To explore the effects of the nerve growth factor (NGF) on N-methyl-D-asparate receptor 1 (NMDAR 1) after spinal cord injury. METHODS: Spinal cord injury of Wistar rats was performed with Allen's method by a 10 gx2.5 cm impact on the posterior T8 spinal cord. NGF was given to the rats of the treatment group via subarachnoid space tube at once, 2, 4, 8, 12 and 24 hours after spinal cord injury, respectively. The expression of NMDAR1 mRNA in spinal cord was detected by in situ hybridization. RESULTS: Rare expression sequence of NMDAR1 mRNA was found in rat spinal cord of the normal group. A strong expression sequence of NMDAR1 mRNA was found in rat spinal cord of the normal saline group. The expression of NMDAR1 mRNA in the NGF group was significantly decreased as compared with that in the normal saline group (P=0.01). CONCLUSIONS: NGF can relieve damage of injured spinal cord by prohibiting the expression of NMDAR1 mRNA.     

胆—肠Roux—Y吻合术的长期效果

胆－肠Ｒｏｕｘ－Ｙ吻合术的长期效果林擎天王洪胆－肠Ｒｏｕｘ－Ｙ吻合术是胆道外科常用的一种内引流手术。它的适应症较广，可用于良性或恶性胆道疾病。现为总结其长期效果，本文仅就我院１５年来对４７例良性胆道疾病使用的情况报导如下：临床资料１一般资料：全组４７...     

C—erbB—2和波形蛋白在乳癌表达及临床意义分析

Ｃ－ｅｒｂＢ－２和波形蛋白在乳癌表达及临床意义分析辽宁省人民医院病理科（１１００１５）景士兵魏少忠任秋华王泽兴中国医科大学第二临床学院病理科（１１０００３）何小明沈阳市红十字会医院外科（１１００４３）吴星恺近年来研究报告表明Ｃ－ｅｒｂＢ－２在乳癌中的...     

IL—1,TNF和IL—6与骨关节炎

骨关节炎患者关节滑液中可检测了高水平的ＩＬ－１，ＴＮＦ和ＩＬ－６，这是参与滑膜炎性病变，关节软骨基质降解及干扰软骨细胞功能的重要细胞因子，对其采取有效的抑制措施可能是早期防治骨关节炎的新途径。