紫外分光光度法测定马钱子中士的宁的含量

目的 用紫外分光光度法测定马钱子提取液中士的宁含量,为士的宁含量测定提供了依据.方法 用紫外分光光度计对士的宁标准溶液进行波长扫描,确定最适吸收波长,在此波长下建立士的宁标准品的吸光度-浓度标准曲线.根据标准曲线测定马钱子提取液中士的宁的含量.结果:士的宁最适吸收波长为254nm,吸光度-浓度回归方程为y=36.425x+0.0039,平均加样回收率为99.21%,RSD为2.24%,马钱子提取液中士的宁含量平均值为833mg·g-1.结论 紫外分光光度法测定马钱子提取液中士的宁含量,方法简单、快速,重现性好,可为士的宁质量控制提供依据.     

导数光谱法测定疏风定痛丸中士的宁的含量

疏风定痛丸是由马钱子、麻黄、乳香、没药、千年健、自然铜、杜仲等 1 5味中药制成的复方蜜丸制剂[1 ] 。具有祛风散湿、活血止痛的功效。由于君药马钱子中含有毒性较大的士的宁 ,因此《中华人民共和国药典》规定了士的宁的含量范围。疏风定痛丸中士的宁的分析测定方法很多 ,药典采用了高效液相色谱法代替以前一直沿用的薄层 -比色法 ,然而在实际应用中 ,该方法存在着提取过程烦琐等缺点。本实验针对上述问题采用导数光谱法直接测定样品中的士的宁含量 ,结果较为满意。1　仪器、药品与试剂岛津 UV-2 40 1型紫外分光光度计 ;士的宁对照品由…     

马钱子制剂中士的宁含量测定方法研究进展

马钱子为马钱科植物马钱(Strychnos nux-uomica L.)的干燥成熟种子。为常用的剧毒中药,具有通络止痛、散结消肿功效。所含的主要有效成分和有毒成分为士的宁,为确保该成品安全有效,必须采用可靠简便的含量测定方法严格控制士的宁的含量。笔者对不同剂型中药制剂中士的宁含量测定     

现代分析方法测定马钱子中士的宁和布鲁生的含量

士的宁和布鲁生是马钱子中主要成分,马钱子中士的宁含量的测定方法有紫外分光光度法、双波长分光光度法和薄层色谱法等。本文采用三波长分光光度法同时测定马钱子中士的宁和布鲁生两种主要成份的含量.1 仪器与试剂(1)岛津 uv-300型分光光度计;(2)MILTON ROY SP1001型分光光度计(以下简称 MRSP1001):(3)士的宁和布鲁生对照品(中国药品生物制品检定所提供);(4)制马钱子粉(江苏省中医院提供)。2 实验条件的选择(1)吸收光谱的绘制:士的宁和布鲁生均     

龙马胶囊中士的宁的含量测定

龙马胶囊中士的宁的含量测定福建省中医药研究院（福州３５０００３）陈玲福建中医学院屏山制药厂（福州３５０００３）王玉兰福州市中医院（福州３５０００１）李志忠龙马胶囊是由马钱子、白术等几味中药制成的中药制剂，用于治疗肿瘤。马钱于是解毒、止痛之要药，其有效...     

通脉丸溶出液中士的宁和马钱子碱的含量测定

目的:建立通脉丸溶出液中士的宁和马钱子碱的含量测定方法。方法:采用超高效液相色谱法测定通脉丸溶出液中士的宁和马钱子碱的含量,色谱条件:Acquity UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm)色谱柱,以乙腈-0.02 mol·L~(-1)磷酸二氢钾与0.014 8 mol·L~(-1)庚烷磺酸钠等量混合溶液(用体积分数10%磷酸调节PH为2.8)(17∶83)为流动相;检测波长为260 nm;流速为0.3 m L·min~(-1);柱温30℃。结果:本方法对通脉丸溶出液中士的宁与马钱子碱均能适用,其中马钱子碱在3.712~74.24μg、士的宁在3.472~69.44μg内呈良好线性关系,r~2=0.999 9,平均加样回收率分别为102.65%(RSD 1.08%),104.19%(RSD 1.46%)。结论:该法分离效果好,专属性强,操作简便快捷,适用于通脉丸溶出液中马钱子碱与士的宁的测定。     

乌头碱类中毒致严重心律失常28例临床分析

乌头碱类中毒症状重，特别是对心脏毒性较强，现报道笔者遇到乌头碱类中毒致严重心律失常的治疗体会。１临床资料２８例患者男２０例、女８例，年龄１９～７６岁；服用伸筋丸６例、舒筋活血丸６例、大活络丸９例、小活络丸５例、含附子中药煎剂２例；服毒９例、误服１１例...     

分光光度法测定马钱子中士的宁含量时共存组分的干扰考察方法

建立了分光光度法测定药材中单一成分含量时 ,共存组分干扰的考察方法。考察了药典载马钱子中士的宁的含量测定方法 ,表明共存物的干扰占其含量的 5 0 %以上。并用薄层层析 -分光光度法和药典法测定了士的宁的含量 ,结果与考察方法一致。     

对油炸前后马钱子中士的宁含量的测定

本文测定了油炸前后马钱子中士的宁的含量，实验结果表明，油炸前和油炸后的马钱子中有效成分士的宁的含量有较大差别。     

HPLC法测定东乐膏中马钱子生物碱的含量

应用HPLC法(高压液相法)测定是东乐膏中士的宁、马钱子碱的含量,为修订质量标准、保证药品质量提供依据。线性回归方程：士的宁y=1841258x 5823,r=0．9987,马钱子碱y=1040171x 5314,r=0．9971;精密度：士的宁RSD=1.8％,马钱子碱RSD：2．0％.测定991101、991102、991129三个批次东乐膏中士的宁的含量(μg/片)分别为：398.2、534.8、428.6：马钱子碱的含量(μg／片)分别为：213.0、307.3、266．8,回收率测定平均为：士的宁102．2％,马钱子碱98．2％。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.