蚌埠市献血员血清铁,铜,锌含量测定

本文采用原子吸收分光光度法测定了蚌埠市健康成人血清中铁、铜及锌的含量。测定结果:铁16.01～32.74μmol/L,铜14.70～31.93μmol/L、锌14.21～23.38μmol/L,本文还阐述了上述三种必需微量元素以酶的形式参与人体生化反应促进机体的物质代谢。     

冠心病患者血清铜锌含量测定的临床意义

应用火焰原子吸收分光光度法对98例冠心病患者及41例健康人血清中铜、锌的含量进行了测定,其中冠心病高胆固醇患者51例;冠心病高甘油三酯患者44例,结果冠心病高胆固醇组血清铜含量为13.10±1.93μmol/L;血清锌含量为19.66±4.07μmol/L;铜锌比值为0.68±0.14。冠心病高甘油三酯组血清铜含量为12.83±2.67μmol/L;血清锌含量为18.22±5.29μmol/L;铜锌比值为0.73±0.15。与对照组相比,冠心病两组血清铜、铜锌比值显著降低(P<0.01);血清锌含量明显升高(P<0.01),提示血清铜、锌含量,尤其是铜锌比值测定是诊断冠心病的一项客观指标,同时也是冠心病易患因素之一。     

冠心病患者血清锌、铜含量的测定及其临床意义

应用火焰原子吸收分光光度法对53例冠心病患者及50例健康人血清中锌、铜的含量进行了测定。结果冠心病组血清铜含量X=15.99±2.64μmol/L;血清锌含量X=19.62±3.78μmol/L。铜/锌比值X=0.83±0.22。与健康人对照组相比,血清铜、铜/锌比值明显降低,血清锌含量显著升高(P均<0.001)。提示血清锌、铜含量测定是诊断冠心病的一项指标。     

妇科恶性肿瘤130例血清微量元素测定分析

目的探讨血清铜、铁、锌、钙、锰的含量与妇科恶性肿瘤的关系。方法采用原子吸收分光光度法,对经组织病理学确诊的130例妇科恶性肿瘤患者血清铜、铁、锌、钙、锰进行测定,并与本院普查的健康女性260例进行对照。结果 25例乳腺癌患者、26例子宫内膜癌患者、38例卵巢癌患者、41例宫颈癌患者血清铜含量分别为（20.44±2.86）、（17.54±3.85）（、6.84±2.21）和（14.23±3.15）μmol/L,与正常对照组的（12.73±2.56）μmol/L比较均明显增高（P〈0.05）;血清铁含量分别为（13.59±2.58）（、11.87±2.54）、（15.44±3.04）和（12.45±2.62）μmol/L;锌含量分别为（5.21±2.06）、（3.88±2.14）、（4.55±1.89）和（5.74±2.11）μmol/L,均低于正常对照组的血清铁含量（19.45±5.34）μmol/L和血清锌含量（7.25±6.73）μmol/L（P〈0.05）;血清钙、锰与正常组比较无明显差异（P〉0.05）。结论血清铜、铁、锌与妇科恶性肿瘤的发生有关,这为妇科恶性肿瘤的防治提供了新的思路。     

火焰原子吸收法测定冠心病患者血清锌、铜含量及临床意义

应用混合酸消化法处理血清样品,对47例冠心病患者及46例健康人血清锌、铜进行了火焰原子吸收测定。结果表明:冠心病组血清铜含量(?)=14.32μmol/L, SD=2.91μmol/L;血清锌含量(?)=19.64μmol/L.SD=3.73μmol/L。铜/锌=0.73±0.21,与健康人对照组相比,血清铜、铜/锌明显降低,血清锌含量显著升高。其差异有高度显著性(p<0.001)。提示血清锌、铜含量的测定是诊断冠心病的一个重要参数。     

泉州地区466名儿童血清锌、铜、铁含量分析

泉州地区466名健康儿童血清锌含量13.56±6.60μmol/L,血清铜17.8±8.12μmol/L,铜/锌比值为1.31,339名儿童血清铁含量19.48±9.27μmol/L。饮用不同水源儿童血清锌、铜、铁含量无显著差异。     

微量法检测成都市1～3月婴儿的血清锌铜含量

本文报道示波极谱法检测婴儿外周血血清锌铜含量的微量测定方法。用该法检测了成都市243名1～3月正常婴儿的血清锌铜含量,其分布均呈正偏态,无性别及月龄的差异。本市1～3月婴儿血清锌的参考值范围为6.69～25.4μmol/L,血清铜的参考值范围为8.68～28.08μmol/L。     

喉癌患者血清和喉组织中铜、锌含量的分析

用原子吸收光谱仪测定了45例喉癌病人血清和喉癌组织及其癌旁组织中铜、锌含量。同时检测了28例健康人血清铜、锌含量。结果喉癌病人血清铜含量(1.94±2.83μmol/L)、铜/锌比值(1.29±0.33)高于正常对照值(血清铜15.39±2.36μmol/L、铜/锌比值0.87±0.18);喉癌病人血清锌含量(15.76±3.67μmol/L)低于正常对照值(17.75±3.21μmol/L);喉癌病人血清铜含量和铜/锌比值随着喉癌进展而增高;喉癌组织中铜含量及铜/锌比值高于其癌旁组织,差异均有显著意义。结果表明:铜,锌与喉癌的发生,发展可能存在一定的相互关系;血清铜含量及铜/锌比值对喉癌的分期有一定的辅助价值。     

重庆市6 681例儿童血清中部分微量及宏量元素情况分析

目的了解重庆市儿童血清中锌、铁、铜、钙、镁的水平。方法用日立-50型原子吸收分光光度计检测并分析近2年来来我院检查的6 681例0~14岁儿童的血清锌、铁、铜、钙、镁含量及性别、年龄分布特点。结果6 681例儿童的血清锌、铁、铜、钙、镁的含量分别为(15.3±4.5)μmol/L(、19.2±6.3)μmol/L(、18.4±4.4)μmol/L(、2 255.4±177.0)μmol/L和(836±109.7)μmol/L,且男性儿童高于女性儿童,差异有统计学意义。女性儿童明显较男性儿童更易发生锌、铁、钙缺乏的情况。0~3岁儿童血清锌高于3岁及3岁以上儿童。1岁以下儿童血清铁、钙高于1岁及1岁以上儿童。血清锌、铁、钙出现缺乏的可能性随年龄的增加而增加。过量的情况除血清铜外都较少发生,且血清铜过量无性别差异;1岁以上儿童铜过量的发生率高于1岁以下儿童,但不随年龄变化而增加。结论儿童应根据其不同的年龄、性别,有所侧重的、科学合理的预防有益元素的缺乏及过量。     

血清CA125和铜,锌测定诊断盆腔良恶性包块的比较和探讨

本文对100例疑为卵巢肿瘤的盆腔包块患者在剖腹探查前测定其血清CA125、铜、锌和铜/锌比值.结果表明:如以血清CA125≥65u/ml、铜≥24.09μmol/L和铜/锌比值≥1.77为界值,其诊断恶性包块的敏感性分别为80.65%、51.62%和48.39%.特异性分别为93.75%、98.44%和96.88%;经采用逐步判别分析表明,虽然联合应用血清CA125、铜和铜/锌比值诊断恶性包块的敏感性可达87.10%,但与血清CA125测定比较其诊断价值无显著性差异.此外,本文还对其他32例妇科恶性肿病进行了术后随访监测,结晨表明,血清CA125测定能有效地监测卵巢上皮性癌,但对生殖或性索间质肿瘤则无监测意义;而铜、锌或铜/锌比值测定则在任何盆腔恶性肿瘤监护中均无任何价值。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.