CXCR4在食管鳞癌组织中的表达及临床意义

目的探讨趋化因子受体CXCR4在食管鳞癌组织中的表达及其与食管鳞癌发生、发展的关系。方法采用RT-PCR及Western blot法检测35例食管鳞癌及其癌旁对照组织中CXCR4的表达水平。结果CXCR4 mR-NA及蛋白在食管鳞癌组织中的表达水平显著高于癌旁对照组（P〈0．05）,癌组织中淋巴结转移组的CXCR4 mR-NA及蛋白表达显著高于无淋巴结转移组（P〈0．05）;癌组织中CXCR4蛋白在侵及外膜组高于未侵及外膜组（P〈0．05）,在Ⅲ期的表达高于Ⅱ期（P〈0．05）。结论趋化因子受体CXCR4与食管鳞癌的发生、侵袭和转移密切相关,可能成为抑制食管鳞癌侵袭转移的新靶点。     

食管鳞癌中核转录因子-κB和CXCR4的表达及意义

目的探讨核转录因子-κB(NF-κB)和CXCR4在食管鳞癌发生、发展中的作用。方法应用免疫组化SP法检测124例食管鳞癌组织、62例癌旁不典型增生组织及62例正常食管组织中NF-κB和CXCR4蛋白的表达情况。结果 NF-κB和CXCR4蛋白在正常食管黏膜、癌旁不典型增生组织和食管鳞癌组织中的阳性表达率逐渐增高(P<0.05)。NF-κB和CXCR4蛋白的表达与食管鳞癌的浸润深度和淋巴结转移情况均密切相关(P<0.05),而与患者的性别、年龄无显著相关性(P>0.05),同时NF-κB和CXCR4蛋白表达间呈正相关关系(P<0.05)。结论 NF-κB和CXCR4的高表达与食管鳞癌的发生及浸润、转移密切相关,二者可能协同促进了食管鳞癌的转移。     

食管鳞状细胞癌组织JNK1蛋白和mRNA的表达及意义

采用免疫组化SP法和逆转录—聚合酶链反应法分别检测40例食管鳞状细胞癌组织和癌周正常食管黏膜组织中氨基未端激酶（JNK1）蛋白及其mRNA的表达。结果癌周正常食管黏膜组织JNK1蛋白及JNK1 mR-NA的阳性表达率均低于癌组织（P均〈0.05）。食管鳞状细胞癌组织中JNK1蛋白和mRNA的表达与癌组织的分化程度和浸润深度有关（P〈0.05）,与性别、年龄及淋巴结转移无关（P〉0.05）。提示JNK1可能在食管鳞癌的恶性发展过程中起一定的作用。     

Bmi-1和S100A4蛋白在食管鳞状细胞癌中的表达及其临床病理意义

目的:探讨Bmi-1和S100A4蛋白在食管鳞状细胞癌(ESCC)组织中的表达及其临床病理意义.方法:采用免疫组织化学法分别检测68例食管鳞状细胞癌、45例癌旁异型增生及36例正常食管黏膜组织中Bmi-1及S100A4蛋白的表达,并分析两者的表达水平与临床病理因素的关系.采用X2检验进行统计学分析.结果:Bmi-1蛋白在食管鳞状细胞癌、癌旁异型增生及正常黏膜组织中的阳性表达率分别为57.4%、48.9%、25.0%;S100A4蛋白的阳性表达率分别为48.6%、26.7%、13.9%,两者在组间的表达差异有统计学意义(P<0.01).食管鳞癌组织中Bmi-1和S100A4的蛋白表达与淋巴结转移及TNM分期均密切有关(P<0.05),而S100A4的蛋白表达还与肿瘤浸润深度有关(P<0.05).两者在食管鳞状细胞癌组织中的表达呈正相关(r=0.302,P<0.05).结论:Bmi-1及S100A4的蛋白表达与食管鳞状细胞癌发生发展密切相关,联合检测Bmi-1及S100A4两蛋白指标对食管鳞癌的预后判断具有重要的意义.     

食管鳞状细胞癌组织中RSK4、p63的表达变化及意义

目的 探讨食管鳞状细胞癌（鳞癌）组织中核糖体S6蛋白激酶4(RSK4)、p63的表达变化及意义。方法收集食管鳞癌活检标本72例份,应用免疫组织化学EnVision两步法检测癌组织中RSK4、p63蛋白表达,染色结果采用半定量分析。选取食管鳞状细胞癌细胞系TE-11、TE-10及正常食管细胞Het-1A,分别采用qRT-PCR法、Western blotting法检测RSK4、p63 mRNA及蛋白表达。采用Kaplan-Meier法及Cox风险回归模型对患者进行生存分析。结果食管鳞状细胞癌组织中RSK4蛋白阳性表达率为62.5%(45/72),p63蛋白阳性表达率为83.3%(60/72)。食管鳞状细胞癌细胞系（TE-11、TE-10）中RSK4、p63基因的mRNA和蛋白表达均高于正常食管细胞Het-1A(P均<0.05)。Kaplan-Meier法生存分析结果显示,RSK4阳性表达与食管癌患者预后不良相关（P<0.05）,且分期、组织分化程度、淋巴结转移等均是影响患者生存期的因素（P均<0.05）;p63表达与患者临床病理特征及预后均无关（P均>0.05）。...     

TMEM16A在食管鳞癌中的表达及意义

目的: 探讨TMEM16A在食管鳞状细胞癌发生发展中的意义.方法: 2007-01/2008-04南京医科大学附属江宁医院手术切除的食管鳞癌标本60例, 均为癌组织及距癌至少5 cm远的配对癌旁组织. 应用免疫组织化学SP法及Western blot法检测食管鳞癌组织及相应癌旁组织的TMEM16A蛋白表达.结果: 免疫组织化学结果: 食管鳞癌组织、癌旁组织中TMEM16A蛋白的表达差异具有统计学意义(52.50% vs 20.00%, P<0.05),TMEM16A在淋巴结转移组中的阳性表达率显著高于无淋巴结转移组(65.38% vs 21.43%,P<0.05), 不同分化程度高分化、中分化、低分化的食管鳞状细胞癌中, TMEM16A的阳性表达率分别为27.27%、43.75%和84.62%,3者比较差异有显著性( P<0.05). TMEM16A表达与食管鳞癌的TNM分期无明显相关性;Western blot法检测发现TMEM16A在食管癌组织的表达较癌旁组织强( t = 42.16, P<0.05).结论: TMEM16A可能在食管癌的发生中有一定的作用, 有望为食管癌的分子诊断、基因治疗和预后评估提供新的分子靶点.     

食管鳞癌组织糖代谢酶活性变化及意义

目的 探讨食管鳞状上皮癌组织中糖代谢酶活性变化及与肿瘤生物学行为的关系.方法 用比色法测定30例食管鳞状上皮癌组织与癌旁正常食管黏膜组织中己糖激酶（HK）、苹果酸脱氢酶（MDH）、乳酸脱氢酶（LDH）、葡萄糖-6-磷酸脱氢酶（G6PD）活性,并分析四种酶活性与肿瘤临床病理特征的关系.结果 HK、MDH、LDH、G6PD活性在食管鳞状上皮癌组织中较正常食管黏膜组织中显著升高（P＜0.01）;HK、MDH活性与肿瘤T分期、淋巴结转移无关（P＞0.05）,LDH、 G6PD活性与肿瘤T分期相关（P＜0.05）、与淋巴结转移无关（P＞0.05）;HK、MDH、LDH、G6PD活性均与体质量下降无关（P＞0.05）.结论 食管鳞状上皮癌组织无氧代谢随浸润深度进展而增强;有氧代谢及无氧代谢酶活性均与食管鳞状上皮癌的淋巴结转移、体质量下降无明显关系.     

Barrett食管进展到食管腺癌的凋亡机制

目前已公认Barrett食管（BE）演变为腺癌要经过上皮化生-异型增生-腺癌3个阶段。食管腺癌的发生、发展不仅存在细胞的增殖和分化的异常，同时也存在细胞凋亡异常。目前已发现的在BE进展为食管腺癌过程中可能有重要作用的细胞凋亡调节因子主要有：（1）fas／fasL；（2）TRAIL（肿瘤坏死因子相关凋亡诱导配体）；（3）bcl-2基因家族；（4）survivin；（5）p53；（6）caspase：（7）其他，如胆汁酸受体fxr等。     

新疆哈萨克族食管鳞癌中微小RNA let-7的表达及病理生物学意义

目的探讨微小RNA（miRNA）let-7在食管鳞癌细胞及组织中的表达,及let-7与食管鳞癌临床病理的关系。方法在细胞水平,运用实时荧光定量聚合酶链反应（real-time quantitative PCR,qRT-PCR）检测let-7在正常食管鳞状上皮细胞Heepic及食管鳞癌细胞CaES-17、Eca109和KYSE-150中的表达差异;在组织水平,采用qRT-PCR技术检测let-7在15对哈萨克族食管鳞癌组织和癌旁正常组织中的表达,并分析其与食管鳞癌浸润、转移等临床病理的关系。结果 qRT-PCR结果显示,let-7在3个食管鳞癌细胞系中的表达明显低于在正常食管鳞状上皮细胞Heepic中的表达,let-7在食管鳞癌组织中的表达明显低于其在癌旁正常组织中的表达,其相对表达量比值为0.79±0.56,差异有统计学意义（P〈0.05）;let-7在不同性别、分化程度、大体分型、浸润深度及淋巴结转移之间的相对表达量比值均无统计学差异（P〉0.05）。结论 Let-7在正常食管鳞状上皮细胞和食管鳞癌细胞中均有表达,但在食管鳞癌细胞中呈低表达;let-7在食管鳞癌组织和正常食管鳞状上皮组织中均有表达,但在癌组织中呈显著低表达;哈萨克族食管鳞癌患者let-7低表达与其临床病理参数之间无显著相关性;let-7对哈萨克族食管鳞癌的发生起抑制作用,是食管鳞癌的潜在分子标记物。     

食管鳞癌中Paxillin mRNA的表达及其与癌转移的关系

目的探讨Paxillin mRNA表达与食管癌转移的关系。方法采用原位杂交方法检测54例食管鳞癌、癌旁不典型增生组织及其正常食管组织中Paxillin mRNA的表达。结果有淋巴结转移的21例（A组）食管鳞癌组织、癌旁不典型增生组织和正常食管组织中Paxillin mRNA阳性表达率分别为90．48%（19／21）、52．38％（11／21）和0，无淋巴结转移的33例（B组）食管鳞癌组织、癌旁组织和正常食管组织中的Paxillin mRNA阳性表达率分别为33．33％（7／33）、23．80％（5／33）和0。两组癌组织、癌旁不典型增生组织中Paxillin mRNA阳性表达率比较，P均〈0．05。结论Paxillin mRNA表达与食管鳞癌的转移密切相关。     

膜联蛋白A2在食管鳞状细胞癌中的表达及其与浸润转移的关系

目的 探讨膜联蛋白A2(Annexin A2)在人食管鳞状细胞癌(ESCC)中的表达变化与浸润转移的关系.方法 收集新疆医科大学第一附属医院2000年至2008年间,手术切除并经病理证实的ESCC组织作为实验组、对应癌旁5cm以上的组织作为对照组;随机选取了ESCC与癌旁配对的22对新鲜组织和175对石蜡包埋组织作为研究对象.应用实时荧光定量PCR(real-time quantitative PCR,qRT-PCR)、蛋白免疫印迹和免疫组织化学的方法,从mRNA和蛋白两个方面检测Annexin A2的表达,并分析其与临床病理参数的关系.结果 在22对新鲜ESCC与癌旁组织中,Annexin A2在癌旁组织中的mRNA表达量(0.06±0.06)显著高于癌组织(0.02±0.02,P<0.05);Annexin A2在癌旁组织中的蛋白表达量(0.95±0.42)高于癌组织(0.81±0.36,P<0.05).在175对ESCC与癌旁组织石蜡标本中,Annexin A2蛋白的阳性表达率为82.3%(144/175),低于配对的癌旁组织中的阳性表达率[92.0%(161/175),P<0.05].此外,Annexin A2的表达与ESCC患者的淋巴结转移及分化程度相关(P<0.05),而与ESCC患者的大体类型关系不明显(P＞0.05).结论 Annexin A2在ESCC中的低表达可能对ESCC的发生发展及浸润转移起着一定的作用.     

TESTIN和Caspase-3蛋白表达与食管鳞癌临床病理特征及预后的关系

目的 检测食管鳞癌中TESTIN基因和Caspase-3蛋白表达水平,及与食管鳞癌临床病理特征及预后的关系.方法 应用免疫组化法检测50例食管鳞癌及癌旁＞5 cm组织中的TESTIN蛋白和Caspase-3蛋白的表达水平.Western印迹及半定量RT-PCR法检测65例配对人食管鳞癌和癌旁组织中TESTIN表达的差异.分析两因素表达的相关性及其与食管鳞癌临床病理特征及预后的关系.结果 50例食管鳞癌标本中,免疫组化结果显示TESTIN蛋白和Caspase-3蛋白阳性率分别为30.0%(15/50)和24.0%(12/50),显著低于癌旁组织的84.0%(42/50)和94.0%(47/50),组间差异有统计学意义(P值均＜0.01).65例配对人食管鳞癌组织中,TESTIN mRNA的表达量显著低于癌旁组织(P＜0.05);Western印迹检测结果显示,69.2%(45/65)的食管鳞癌组织TESTIN蛋白表达量比对应癌旁组织下降(P＜0.01).TESTIN的表达与食管鳞癌分化程度有关,而与性别、年龄、肿瘤大小、TNM分期、淋巴结转移无关.食管鳞癌中TESTIN在蛋白水平与Caspase-3表达呈正相关.TESTIN蛋白阴性表达者的累计生存时间显著低于阳性者(P＜0.05).结论 TESTIN和Caspase-3在食管鳞癌组织中的表达下调且呈正相关性,两者在食管鳞癌的发生发展过程中可能起协同作用,TESTIN对评价食管鳞癌的预后可能有较好价值.     

Persistent CXCR4 expression after preoperative chemoradiotherapy predicts early recurrence and poor prognosis in esophageal cancer

INTRODUCTIONThe migration of tumor cells to a secondary site from their primary location is a crucial issue in cancer metastasis. Recently, a novel “homing” signaling mechanism has been proposed, in which target organs produce and release specific chemo…     

Association between Bmi1 and clinicopathological status of esophageal squamous cell carcinoma

AIM： To investigate the clinicopathological roles of Bmil in esophageal squamous cell carcinoma （ESCC）.METHODS： Quantitative real-time polymerase chain reaction and immunohistochemical staining for Broil were performed in cancerous and adjacent non-cancerous paraffin-embedded esophageal specimens.RESULTS： The Bmil expression level was unaffected by gender and age. The level of Broil mRNA in ESCC was significantly higher than that in the adjacent non-cancerous tissues （2.181 ± 2.158 vs 0.931 ± 0.894, P = 0.0152）, and its over-expression was aggressively associated with lymph node metastasis （3.580 ± 2.487 vs 1.703 ± 0.758, P = 0.0003）, poorer cell differentiation （P = 0.0000） and advanced pathological stage （3.827± 2.673 vs 1.590 ± 0.735, P = 0.0001）. The patients were divided into high-expression and low-expression groups based on the median expression level of Bmi1 mRNA, and a shorter overall survival time in the former group was observed. Immunohistochemistry for Bmi1 oncoprotein showed diffusely positive, focally positive and negative expression in 44, 16 and 10 of 70 ESCC cases, respectively, compared with three, two and five of 10 adjacent non-cancerous cases （P = 0.027）. The positive rate of the oncoprotein in samples of histological grade Ⅲ was higher than that of grade Ⅱ（P = 0.031）, but its expression had no relation to the lymph node metastasis and pathological staging. In 70 ESCC samples, Bmi1 showed high intense expression in the cytoplasm and less or even no expression in the nucleus.CONCLUSION： Bmi1 was over-expressed in ESCC. Increased Bmi1 mRNA expression was significantly associated with ESCC progression, and the oncoprotein was largely distributed in the cytoplasm of tumor cells.     

Expression of thymidylate synthase and glutathione-s- transferase π in patients with esophageal squamous cell carcinoma

AIM： To investigate the expression of thymidylate synthase （TS） and glutathione-s-transferase π （GST-π） in esophageal squamous cell carcinoma and their association with the clinicopathologic characteristics. METHODS： Immunohistochemical methods were used to detect the expression of TS and GST-π in surgically resected formalin-fixed, paraffin-embedded esophageal squamous cell carcinoma （ESCC） tissue sections from 102 patients （median age, 58 years） and in 28 normal esophageal mucosa （NEM） samples. The relationship between TS and GST-π expression and clinicopathologic factors was examined. RESULTS： The expression of TS and GST-π was not statistically significantly associated with age of the patients, tumor size, lymph node metastasis, depth of invasion or tumor stage. TS staining was positive in 17.86% of normal esophageal mucosa and in 42.16% of ESCC samples （P 〈 0.05）. The expression level of TS was not only significantly lower in well-differentiated （21.88%） than in poorly-differentiated carcinomas （51.43%, P 〈 0.05）, but was also significantly higher in samples from male patients （46.51%） than from female patients （18.75%, P 〈 0.05）. GST-π was positively stained in 78.57% of normal esophageal mucosa and in 53.92% of ESCC samples （P 〈 0.05）. The expression level of GST-π was also significantly higher in welldifferentiated carcinomas （65.63%） than in poorly- differentiated carcinomas （35.00%, P 〈 0.05）. CONCLUSION： The expression of TS and of GST-π may be used as molecular markers for the characterization of ESCC. Poorly-differentiated cells showed increased expression of T5 and reduced expression of GST-π.     

食管鳞癌中微小核糖核酸let-7的表达及病理生物学意义

目的 探讨微小RNA(miRNA)1et-7对食管鳞癌细胞增殖的影响及食管鳞癌组织中let-7表达水平与临床病理特征间的关系.方法 利用RNA干扰(RNAi)和细胞转染技术将食管鳞癌细胞Eca109分别转染入let-7、let-7抑制剂及随机序列.以正常培养的Eca109细胞为阴性对照组.噻唑蓝(MTT)比色法检测各组Eca109细胞的增殖情况.实时荧光定量聚合酶链反应(qRTPCR)检测各组细胞、45例食管鳞癌组织及其癌旁组织中let-7的表达水平,并分析其与食管鳞癌临床病理特征间的关系.结果 转染后72 h,转染let-7组吸光度(A)值较阴性对照组明显降低(P=0.005),转染let-7抑制剂组A值较阴性对照组明显升高(P=0.029).与阴性对照组let-7表达量比较,转染let-7组表达增加33%(1.33比1.00,P=0.039),转染let-7抑制剂组表达降低50%(0.50比1.00,P=0.014).食管鳞癌组织和癌旁组织中let-7相对表达量的比值为0.66±0.47,差异有统计学意义(P=0.001).汉族患者食管鳞癌组织中let-7相对表达量(0.48±0.43)低于哈萨克族(0.88±0.51,P=0.019).低分化食管鳞癌组织中let-7相对表达量(0.42±0.30)低于高分化食管鳞癌组织(0.84±0.38,P=0.015).有淋巴结转移者食管鳞癌组织中let-7相对表达量(0.50±0.35)低于无淋巴结转移者(0.80±0.52,P=0.032).结论 let-7对食管鳞癌的发生、发展起抑制作用,其表达水平与组织分化程度、淋巴结转移及民族相关.

Abstract：

Objective To estimate the effect of microRNA (miRNA) let-7 expression on human esophageal squamous cell carcinoma(ESCC) and the relationship between let-7 level and clinicopathological parameters. Methods ESCC cell line (Eca109) was transfected with let-7 or its inhibitor by RNAi and cell transfection techniques. Normal cultured Eca109 cell was served as negative control. The proliferation of Eca109 cell was detected by MTT. The expression of let-7 in Eca109 cells and 45 paired ESCC tissues and corresponding para-cancerous tissues were measured using real-time quantitative polymerase chain reaction (qRT-PCR). The relationship between let-7 level and clinicopathological parameters in patients with ESCC was analyzed. Results The A value of let-7 in Eca109 cells transfected with let-7 was lower than negative control (P=0.005), while it was higher in Eca109 cells transfected inhibitor than that in negative control 72 hours after transfection. In comparison with negative control, the expression of let-7 in Eca109 cells transfected with let-7 was increased 33% (1.33 vs 1.00,P=0. 039) and it was decreased 50% in Eca109 cells transfected with inhibitor (0.50 vs 1.00,P=0. 014). The ratio of let-7 expression in ESCC tissue and para-cancerous tissue was 0.66 ± 0.47 with significant differece (P= 0.001). Moreover, The level of let-7 expression in Han patients with ESCC was lower than Kazakh patients with ESCC (0.48±0.43 vs 0. 88±0.51,P=0. 019). The level of let-7 expression in poorly differentiated ESCC tissue was lower than well differentiated ESCC tissue (0.42±0.30 vs 0.84±0.38,P=0. 015). The level of let-7 expression in patients with lymph node metastasis was lower than those without lymph node metastasis (0.50±0.35vs 0. 80±0.52,P=0. 032) . Conclusion It is demonstrated that let-7 can inhibit the carcinogenesis and development of ESCC. The level of let-7 expression is associated with cell differentiation,lymph node metastasis and nationalities.     

P27KiPl在食管良恶性疾病中的表达及与食管癌淋巴结转移的关系

目的 研究 P27KiPl(Kinase inhibitor proteinl,P27)在食管良恶性病变中的表达及意义。方法 以免疫组织化学SP法检测P27在食管增生的鳞状上皮、食管息肉及食管鳞癌中的表达。结果 P27在食管增生上皮、食管息肉及食管癌中的阳性表达率分别为93.3％、100％及48.0%,三者差异有极显著性(P<0.005),良性病变阳性率显著高于恶性肿瘤(P<0.01),而且P27表达状况与食管癌淋巴转移显著相关(P<0.05)。结论 食管癌中P27表达水平显著下降,且P27低表达与淋巴结转移有关。     

肌动蛋白凝胶蛋白在食管鳞癌中的表达及其与临床病理因素的关系

目的探讨肌动蛋白凝胶蛋白(Transgelin)在食管鳞癌组织中的表达及其与临床病理因素间的关系。方法采用免疫组化法检测Transgelin在食管鳞癌组织、癌旁不典型增生组织及正常食管黏膜组织中的表达,并分析Transgelin的表达与患者临床病理因素特征的关系及其对预后的影响。结果Transgelin在食管鳞癌中的阳性率明显高于癌旁不典型增生组和正常食管黏膜组织,其差异有统计学意义(P<0.05)。Transgelin在食管癌中的表达与患者的的TNM分期(P<0.05)、淋巴结转移(P<0.05)、远处转移(P<0.05)有关;与患者的性别、年龄、肿瘤直径无关(P>0.05)。在随访的210例患者中,Transgelin的生存曲线显示,高表达组Transgelin的5年生存率明显低于低表达组(P<0.05)。单因素生存分析显示,Transgelin的表达水平、TNM分期、淋巴结转移、远处转移与食管鳞癌患者术后生存时间相关。同时,多因素Cox比例风险回归模型分析显示,Transgelin的表达水平、TNM分期、淋巴结转移和远处转移为食管鳞癌患者预后的独立危险因素。结论Transgelin在食管鳞癌组织中呈高表达,其表达与食管鳞癌的发生发展和侵袭转移相关,Transgelin对于术后食管鳞癌患者的预后评估具有参考价值。     

Expression of vascular endothelial growth factor-C and angiogenesis in esophageal squamous cell carcinoma

AIM: To investigate the expression of vascular endothelial growth factor-c (VEGF-C) mRNA and microvessel density (MVD) in human esophageal squamous cell carcinoma (ESCC) and its relationship with clinical significance. METHODS: Specimens obtained from 43 patients undergoing surgical resection for ESCC were used in this study. The expression of VEGF-C mRNA was examined by in situ hybridization. Tumor MVD was determined immunohistochemically with anti-CD31 antibody and estimated by image analysis. Ten sections of adjacent normal mucosa were also examined. RESULTS: VEGF-C mRNA expression was detected in cytoplasm of carcinoma cells. Of the 43 ESCC patients studied, 18 cases (41.9%) were positive for VEGF-C mRNA. No VEGF-C mRNA expression was observed in normal esophageal mucosa. VEGF-C mRNA expression correlated significantly with lymph node metastasis, TNM stage and depth of invasion (P < 0.05). Furthermore, histological grade (differentiation) tended to correlate with VEGF-C mRNA expression, but was not statistically significant (P > 0.05). In tumor lesions, the MVD was significantly greater than that in normal esophageal mucosa. MVD correlated significantly with lymph node metastasis, TNM stage and depth of invasion (P < 0.05), but not with histological grade (differentiation) (P > 0.05). Lesions with VEGF-C mRNA expression had a significantly higher MVD than that of those without VEGF-C mRNA expression (P < 0.05). CONCLUSION: VEGF-C plays a role in lymphatic metastasis via lymphangiogenesis and angiogenesis in ESCC. VEGF-C is one of the important predictors of the biological behavior in ESCC.     

食管癌活检标本多药耐药基因表达及意义

目的 研究食管癌活检标本中多药耐药基因（multidrug resistance gene １,ＭＤＲ１基因）和多药耐药相关蛋白（multidrug resistance associatid pritein,ＭＲＰ）表达水平,探讨与食管癌临床特征及化疗的相关性。方法 应用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）技术定量分析标本中ＭＤＲ１基因和ＭＲＰ的表达水平。结果 ５８例标本中,ＭＤＲ１基因阳性表达率为８２．８％（４８/５８）,ＭＲＰ表达率为２９．３％（１７/５８）。