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肉桂中肉桂酸的单扫描示波极谱法测定 总被引:11,自引:2,他引:9
本文报道一种测定肉桂中肉桂酸含量的单扫描示波极谱法。在0.4mol/L氯化铵-0.0025mol/L盐酸(pH2.6±0.1)支持电解质溶液中肉桂酸在-1.35V(vs.SCE)有一极谱还原波。该波的一阶导数峰峰高与肉桂酸浓度在2.0×10~(-6)~1.0×10~(-4)mol/L范围内呈良好线性关系(r=0.999).肉桂的其它成分如肉桂醛、肉桂醇、香豆素等不干扰测定。应用本方法测定广西肉桂中肉桂酸的含量,结果令人满意。 相似文献
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本文建立了胆骨化醇及其制剂的二阶导数差示脉冲极谱法定量测定方法。在2.0×l0-5mol/LKCl底液中,于-1.69V(vsAg/AgCl)处出现一良好的二阶导数差示脉冲极谱峰,在2.0×10-5~1.2×10-4mol/L范围内,胆骨化醇的浓度与其导数峰幅值呈非常显著的线性关系(p<0.01),检测限为8.4×10-9mol/L。方法简便、快速、灵敏,结果准确。 相似文献
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反相高效液相色谱法测定血浆和脑脊液中甲氨蝶呤浓度 总被引:8,自引:0,他引:8
目的:建立测定血浆和脑脊液中甲氨蝶呤的反相高效液相色谱方法。方法:采用ODS不锈钢柱,流动相:甲醇∶0.025mol·L-1磷酸盐缓冲液(21∶79,pH6.70),检测波长:306nm,样品经沉淀蛋白质后直接进样。结果:血浆和脑脊液样品回收率分别为97.5%±4.1%和100.8%±1.5%,日内和日间误差(RSD)均<7%,最低检测浓度4.75×10-8mol·L-1,血浆中在1.10×10-7~2.2×10-6mol·L-1和2.20×10-6~1.76×10-4mol·L-1;脑脊液中在1.10×10-7~2.20×10-6mol·L-1和2.20×10-6~1.10×10-3mol·L-1浓度范围内有良好线性关系,r=0.9996~0.9999。结论:本法简便、准确、灵敏。 相似文献
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诺氟沙星的吸附伏安法研究 总被引:5,自引:0,他引:5
在pH=7.49氨-氯化铵缓冲液中,诺氟沙星在滴汞电极上产生一灵敏的还原峰,峰电位为-1.52V(vs.SCE)。在1.98×10(-7)~4.88×10(-6)mol/L之间有良好的线性关系。本文对其极谱性质和电极过程进行了研究,用于诺氟沙星的测定,方法简便,结果准确、可靠。 相似文献
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采用反相高效液相色谱法,选择苯酚为内标物,ZY1104型XWG-C18柱(4.6mm×250mm)为分析柱,甲醇-水-0.2mol/L硫酸铵(10.680.72,pH=7.7)为流动相,流速为1.0mL/min,检测波长为254nm,进样量20μL,测定片剂中盐酸莫索尼定的含量,按盐酸莫索尼定计算,理论板数为2544,样品峰与内标峰的分离度为3.9.线性范围为4~100mg/L(r=0.9998),最低检测量为10ng,平均回收率为99.82%,RSD=0.79%.用该法测定3批盐酸莫索尼定片剂的含量,结果分别为(96.8±1.9)%、(99.4±1.7)%和(99.3±1.6)%.本法快速、灵敏、准确,专属性高. 相似文献
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高效液相色谱法测定血浆中尼麦角林体内代谢物10α—甲氧基—9,10—?… 总被引:1,自引:0,他引:1
采用高效液相色谱法测定人血浆中10α-甲氧基-9,10-二氢麦角醇(MDL,尼麦角林体内代谢物)的浓度。MDL和麦角新碱(内标)有兴亡 从血浆中提取,并在SphersorbC18柱上,以乙腈-0.1mol/L(pH5)醋酸铵(1:4)为流动相进行分离。在225nm处检测。本法简便、灵敏、专属性好。 相似文献
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将离子敏感场效应晶体管(ISFET)与药物敏感膜相结合,研制成一种对麻黄三有良好响应的药物敏感场效应晶体管传感器(DrugFET)。四苯硼钠作为电活性物质,不必转型,民PVC膜IS-FET,对盐酸麻黄碱的线性响应范围,1.0×10^0-9.0×10^-5mol/L,斜率58.8mv/PC(C=mol/L),检测下限3.0×10^-5mol/L,pH5.0-8.0。用其分析麻黄碱片剂的含量,结果与药 相似文献
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Clinical and in vitro investigations were carried out to test the efficacy of gut lavage, hemodialysis, and hemoperfusion in the treatment of poisoning with paraquat or diquat. In a patient suffering from diquat intoxication 130 times more diquat was removed by gut lavage 30 h after ingestion than was removed by complete aspiration of the gastric contents.Determination of in vitro clearances for paraquat and diquat by hemodialysis showed that, at serum concentrations of 1–2 ppm, such as are frequently encountered in poisoning in man, toxicologically relevant quantities of herbicide cannot be removed from the body. At a concentration of 20 ppm, on the other hand, hemodialysis proved to be effective, the clearance being 70 ml/min at a blood flow rate of 100 ml/min. The efficacy of hemoperfusion with coated activated charcoal was on the whole better. Especially at concentrations around 1–2 ppm, the clearance values for hemoperfusion were some 5–7 times higher than those for hemodialysis.In a patient suffering from paraquat poisoning, both hemodialysis as well as hemoperfusion were carried out. The in vitro results could be confirmed: At serum concentrations of paraquat less than 1 ppm no clearance could be obtained by hemodialysis while by hemoperfusion with activated charcoal quite high clearance values were measured and the serum level dropped down to zero.
Zusammenfassung Klinische Untersuchungen und Laboratoriumsversuche wurden durchgeführt, um die Wirksamkeit von Darmspülung, Hämodialyse und Hämoperfusion bei Paraquat- und Deiquat-Vergiftungen zu prüfen.Bei einem Patienten wurde 30 Std nach Deiquat-Aufnahme durch Darmspülung 130mal mehr Deiquat entfernt als durch vollständige Aspiration des Mageninhaltes. In vitro-Versuche ergaben, daß bei Blutserumkonzentrationen von 1–2 ppm, die bei Vergiftungen oft gemessen werden, durch Hämodialyse keine toxikologisch relevanten Paraquat- oder Deiquat-Mengen entfernt werden können. Dagegen erwies sich die Hämodialyse bei 20 ppm und einer Blutumlaufgeschwindigkeit von 100 ml/min mit einer Clearance von 70 ml/min als wirksam. Die Hämoperfusion mit beschicheter Aktivkohle war in diesen Versuchen aber eindeutig überlegen, denn insbesondere bei Konzentrationen um 1–2 ppm waren die Clearance-Werte 5–7mal höher als bei der Hämodialyse.Die in vitro-Ergebnisse wurden bei einem Patienten mit einer Paraquat-Vergiftung bestätigt: Bei Konzentrationen unter 1 ppm war die Hämodialyse wirkungslos, während durch Hämoperfusion relativ hohe Clearance-Werte erreicht wurden, so daß der Serumspiegel rasch unter die Nachweisgrenze abfiel.相似文献
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Petrikovics I McGuinn WD Sylvester D Yuzapavik P Jiang J Way JL Papahadjopoulos D Hong K Yin R Cheng TC DeFrank JJ 《Drug delivery》2000,7(2):83-89
This study describes a new approach for organophosphorous (OP) antidotal treatment by encapsulating an OP hydrolyzing enzyme, OPA anhydrolase (OPAA), within sterically stabilized liposomes. The recombinant OPAA enzyme was derived from Alteromonas strain JD6. It has broad substrate specificity to a wide range of OP compounds: DFP and the nerve agents, soman and sarin. Liposomes encapsulating OPAA (SL)* were made by mechanical dispersion method. Hydrolysis of DFP by (SL)* was measured by following an increase of fluoride ion concentration using a fluoride ion selective electrode. OPAA entrapped in the carrier liposomes rapidly hydrolyze DFP, with the rate of DFP hydrolysis directly proportional to the amount of (SL)* added to the solution. Liposomal carriers containing no enzyme did not hydrolyze DFP. The reaction was linear and the rate of hydrolysis was first order in the substrate. This enzyme carrier system serves as a biodegradable protective environment for the recombinant OP-metabolizing enzyme, OPAA, resulting in prolongation of enzymatic concentration in the body. These studies suggest that the protection of OP intoxication can be strikingly enhanced by adding OPAA encapsulated within (SL)* to pralidoxime and atropine. 相似文献
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《Critical reviews in toxicology》2013,43(5-6):327-369
AbstractThe uptake of metals from food and water sources by insects is thought to be additive. For a given metal, the proportions taken up from water and food will depend both on the bioavailable concentration of the metal associated with each source and the mechanism and rate by which the metal enters the insect. Attempts to correlate insect trace metal concentrations with the trophic level of insects should be made with a knowledge of the feeding relationships of the individual taxa concerned. Pathways for the uptake of essential metals, such as copper and zinc, exist at the cellular level, and other nonessential metals, such as cadmium, also appear to enter via these routes. Within cells, trace metals can be bound to proteins or stored in granules. The internal distribution of metals among body tissues is very heterogeneous, and distribution patterns tend to be both metal and taxon specific. Trace metals associated with insects can be both bound on the surface of their chitinous exoskeleton and incorporated into body tissues. The quantities of trace meals accumulated by an individual reflect the net balance between the rate of metal influx from both dissolved and particulate sources and the rate of metal efflux from the organism. The toxicity of metals has been demonstrated at all levels of biological organization: cell, tissue, individual, population, and community. Much of the literature pertaining to the toxic effects of metals on aquatic insects is based on laboratory observations and, as such, it is difficult to extrapolate the data to insects in nature. The few experimental studies in nature suggest that trace metal contaminants can affect both the distribution and the abundance of aquatic insects. Insects have a largely unexploited potential as biomonitors of metal contamination in nature. A better understanding of the physico-chemical and biological mechanisms mediating trace metal bioavailability and exchange will facilitate the development of general predictive models relating trace metal concentrations in insects to those in their environment. Such models will facilitate the use of insects as contaminant biomonitors. 相似文献
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《Expert opinion on investigational drugs》2013,22(1):79-86
Advances in the molecular biological knowledge of neuronal nicotinic acetylcholine receptors (nAChRs) have led to a growing interest by the pharmaceutical industry in the development of novel compounds that selectively modulate nAChR function. The ability of (-)-nicotine, an activator of nAChRs, to enhance attentional aspects of cognition in animals and humans, to exert neuroprotective and anxiolytic-like effects, and presumably to mediate the negative correlation between smoking and Alzheimer's (and Parkinson's) Disease, has focused interest on the potential therapeutic utility of modulators of nAChR function for treatment of some of the deficits associated with these progressive, neurodegenerative conditions. Numerous compounds are known which activate nAChRs and which might serve as lead compounds toward the development of such agents. The pharmacologic diversity of neuronal nAChR subtypes suggests the possibility of developing selective compounds which would have more favourable side-effect profiles than existing agents. This broader class of agents, collectively called cholinergic channel modulators (ChCMs), is anticipated to encompass compounds which would have more favourable side-effect profiles than existing agents, which generally exhibit low selectivity. This selectivity may be achieved by preferentially activating some subtypes of nAChRs (i.e., Cholinergic Channel Activators, ChCAs) or inhibiting the function of other subtypes (Cholinergic Channel Inhibitors, ChCIs). An overview of the biology of nAChRs and the rationale for the use of ChCMs for the treatment of dementia related to neurodegenerative diseases are presented, followed by a discussion of lead compounds and compounds under consideration for clinical evaluation. 相似文献
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Chang Min Kim Kun Ho Son Sung Hwan Kim Hyun Pyo Kim 《Archives of pharmacal research》1991,14(4):305-310
In order to find out the values of the steroid resources for the future use. the compositions and contents of steroidal sapogenins
from 13 domestic plants have been investigated. As a result,Dioscorea nipponica, D. quinqueloba andSmilax china were found to have large amount of diosgenin. And pennogenin inTrillium kamtschaticum andParis verticillata, yuccagenin inAllium fistulosum, hecogenin inAgave americana and neochlorogenin inSolanum nigum were appeared to be major steroidal sapogenins. 相似文献
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