Detection of hepatitis G virus RNA in hepatitis C patients and hepatitis C virus infected hemodialysis patients

AlthoughsensitiveandspecificimmunoassayandmolecularbiologicaltechniquesforthedetectionofthehepatitisA--Evirusesareavailable,theetiologyofasubstantialfractionofpost--transfusionandcommunity--acquiredhepatitiscasesremainsundefined[1],suggestingtheexistenceofadditionalcausativeagents.Anewhumanhepatitisviruswasisolatedbytwoindependentgroups.ThenewvirusisprovisionallydesignatedashepatitisGvirus(HGV)[ZJorGBvirusC(GBV~C)[3'4),whichwasthoughttobetheagentofpartofthenon--A--Ehepatitispatients.Fro…     

Study of hepatitis C virus genotype in Guizhou area of southwestern China.

ＳｔｕｄｙｏｆｈｅｐａｔｉｔｉｓＣｖｉｒｕｓｇｅｎｏｔｙｐｅｉｎＧｕｉｚｈｏｕａｒｅａｏｆｓｏｕｔｈｗｅｓｔｅｒｎＣｈｉｎａＤｉｎｇＪｉｎｇｊｕａｎ丁静娟，ＴａｋａｄａＡｋｉｒａ高田昭，ＹａｍａｄａＮｏｒｉｋｏ山田规子，ＴｓｕｔｓｕｍｉＭｉｋｉｈｉｒ...     

Hepatitis D virus infection in liver tissues of patients with hepatitis B in China.

2,346 liver samples from 17 cities of China for intrahepatic hepatitis D antigen (HDAg) were studied by direct enzyme-labelled technique. HDAg was detected in 167 out of 1,764 samples of HBsAg positive individuals making a detection rate of 9.47%. Hepatitis D virus (HDV) infection existed in all the examined districts with no significant difference in the HDAg detection rate. It was found that the intrahepatic HDAg detection rate was related to the pathologic type of the liver disease. The HDAg detection rate in chronic liver diseases and severe hepatitis was higher than in other liver diseases. It suggests that HDV infection is associated with the progression and chronicity of the liver disease. Studies on the relationship between HDV infection and HBV replication showed that HBV replication might be suppressed by HDV infection. Both HDV and HBV, however, could replicate in the same hepatocyte simultaneously.

     

Genotyping of hepatitis C virus in patients with hepatocellular carcino Ma

ＧｅｎｏｔｙｐｉｎｇｏｆｈｅｐａｔｉｔｉｓＣｖｉｒｕｓｉｎｐａｔｉｅｎｔｓｗｉｔｈｈｅｐａｔｏｃｅｌｌｕｌａｒｃａｒｃｉｎｏＭａ￥ＣｕｉＸｉａｏｈｏｎｇ（崔晓红）；ＰａｎＷｅｉ（潘卫）；ＱｉＺｈｏｎｇｔｉａｎ（戚中田）；ＳｏｎｇＹａｎｂｉｎ（宋燕斌）...     

Studies on hepatitis C virus and prevalence of its infection in China

PrinceandGoldfieldfirstreportedthenon--A,non--Bpost--transfusionhepatitis(NANBH)byexclusivediagnosisin1974.Theepidemiologicda.talaterconfirmedthatthereweretwokindsofNANBH,parenterallytransmittedNANBHandenterallytransmittedNANBH.'In1989,Chooandhiscolleagues['JidentifiedhepatitisCvirus(HCV)genomiccDNAbyimmunoscreeningtheAgtllcDNAlibraryofthevirus,themajorcausativeagentofparenterallytransmittedNANBH.TheanalysisofthegenomeshowedthatHCVisamemberofflavivirusfamilywithasinglepositiv…     

Detection of hepatitis C virus core, NS3 and NS5 antigens in liver tissues of hepatocellular carcinoma patients

ＤｅｔｅｃｔｉｏｎｏｆｈｅｐａｔｉｔｉｓＣｖｉｒｕｓｃｏｒｅ，ＮＳ３ａｎｄＮＳ５ａｎｔｉｇｅｎｓｉｎｌｉｖｅｒｔｉｓｕｅｓｏｆｈｅｐａｔｏｃｅｌｕｌａｒｃａｒｃｉｎｏｍａｐａｔｉｅｎｔｓＷａｎｇＲｏｎｇｑｕａｎ汪荣泉，ＺｈｏｕＺｉｃｈｅｎｇ周子成，Ｙ...     

Immunohistochemical study of hepatitis E virus antigen in liver tissues from patients with hepatitis E.

Hepatitis E virus antigen (HEVAg) has been identified in liver tissue of 6 out of 14 patients with hepatitis E using anti-HEV-IgG-horseradish peroxidase (HRP) and direct immunoperoxidase staining methods. HEVAg was found only in the cytoplasm of hepatocytes, but nor in the nuclei. The HEVAg positive hepatocytes were scattered singly, but in a few parts, where the liver damage was severe, the HEVAg positive cells were quite concentrated. We also observed the invasion of lymphocytes into the HEVAg positive hepatocytes.These phenomena suggest that the liver damage may be related to the immune reaction. No HEVAg was found in the liver tissues of stillborn fetus and neonate from woman patients with hepatitis E.     

Detection of specific IgM of Cox B virus in patients with acute myocardial infarction

<正> In this paper, the authors determined the specific antibody IgM of Cox B virus in serum of patients with acute myocardial infarction, using SPA absorption, combined with immunoenzyme staining method. Meanwhile, the antibodies were determined with mieromethod of neutralization test.The results showed that in 26 patients with acute myocardial infarction, the specific antibody IgM ratio of Cox B virus was 57.6%, and the double blood tests also showed that patients with acute myocardial infarction was really suffering from the viral infection.     

Assessment of compliance-relevant indispensable knowledge to cope with epilepsy in epileptic patients in Xi＇an

Objective: To investigate the knowledge level related to compliance in patients with epilepsy. Methods: Eighty-seven patients with epilepsy were tested in the city of Xi‘an with a knowledge questionnaire containing 21 questions related to compliance. Results: Over 39. 5% of patients did not know that epilepsy is the result of abnormal firing of neurons, while 29. 9% were uncertain or did not realize that epilepsy attacks can bring up accidents such as traffic accidents, drowning and trauma. A total of 36.6% of responders thought that the best therapy for epilepsy could be found in Traditional Chinese Medicine. As many as 36.8% of tested patients were uncertain or did not know that frequent epilepsy attacks can affect their intelligence. Up to 36% were unaware of the possible consequence of sudden withdrawal of anti-epileptic drugs (AED). Among responders, 39. 1% did not know the right treatment method of epilepsy. That AED can control seizure attacks were doubted in 57.5% of epileptic patients.Furthermore, 32. 2% did not know whether the disease could be cured. Conclusion: In this group of epileptic patients, generally they do not have enough indispensable knowledge to cope with epilepsy. They urgently need for proper health education besides effective AED to control seizure attacks and improve their quality of life.     

Detection of hepatitis C virus RNA sequences in hepatic portal cholangiocarcinoma tisue by reverse t

ＨｅｐａｔｉｔｉｓＣｖｉｒｕｓ（ＨＣＶ）ｉｓａｐｏｓｉｔｉｖｅｓｔｒａｎｄＲＮＡｖｉｒｕｓａｓｏｃｉａｔｅｄｍｏｓｔｌｙｗｉｔｈｐｏｓｔｔｒａｎｓｆｕｓｉｏｎｎｏｎＡ，ｎｏｎＢｈｅｐａｔｉｔｉｓ．１ＰｅｒｓｉｓｔｅｎｔＨＣＶｉｎｆｅｃｔｉｏｎ...     

在西安地区HBeAg阴性患者中检出乙型肝炎病毒前C区基因突变株

作用ＨＢｅＡｇ阴性，抗ＨＢｅ阳性患血清提取ＨＢＶ ＤＮＡ作为模板，用前Ｃ区引物进行聚合酶链反应（ＰＣＲ），选择３份ＰＣＲ扩增阳性产物，用ＥｃｏＲＩ酶切获得长为３１２ｂｐ的基因片段。将其克至ＰＵＣ１８质粒中，转化大肠杆菌ＪＭ１０９，提取质粒，经ＰＣＲ扩增及酶切鉴定后，用双脱氧末端标记法进行基因序列测定。结果发现，在２例患ＨＢＶ ＤＮＡ克隆中存在有前Ｃ区基因突变，即１８９６位的鸟嘌呤为腺嘌呤...     

e抗原阴性重症乙型肝炎患者HBV前C区热点变异研究

目的　研究ＨＢＶ信号肽区热点变异与重症乙型肝炎发生及转归的关系。方法　采用错配聚合酶链反应（ＰＣＲ）和限 制性片段长度多态（ＲＦＬＰ）分析方法检测６８例ＨＢｅＡｇ阴性的重症乙型肝炎病人（其中急性重肝６例、亚急性重肝３８ 例、慢性重肝２４例）及４４例慢性乙型肝炎患者的Ｔ１８６２、Ａ１８９６变异情况。结果　急性重肝的Ａ１８９６、Ｔ１８６２变异分别 为６６．７％（４／６）、０（０／６）;亚急性重肝４２．１％（１６／３８）、１５．８％（６／３８）;慢性重肝 ２５．０％（６／２４）、１６．７％（４／２４）;慢性肝炎 ４５．５％（２０／４４）、２．３％（１／４４）。重症乙型肝炎组与慢性肝炎组比较Ｔ１８６２变异率有显著差异（Ｐ＜０．０１）,Ａ１８９６变异率无显 著差异（Ｐ>０．０５）。结论　提示Ｔ１８６２变异与乙型肝炎的重症化密切相关。而Ａ１８９６变异与乙型肝炎重症化进程是否有 关还不能确定。     

乙型肝炎病毒前C区基因变异的研究

作者应用聚合酶链反应（ＰＣＲ）检测ｅ系统阳性的慢件乙型肝炎（乙肝）患者血清乙肝病毒（ＨＢＶ）ＤＮＡ，筛选出乙肝ｅ抗原（ＨＢｅＡｇ）阳性／乙肝ｅ抗体（抗—ＨＢｅ）阴性和ＨＢｅＡｇ阴性／抗—ＨＢｅ阳性各２０份ＰＣＲ阳性血清，分别用人工合成的ＨＢＶ前Ｃ区基因寡核苷酸探针Ｍ０（无突变），Ｍ１（１点突变），Ｍ２（２点突变）进行斑点杂交，结果发现ＨＢｅＡｇ阴性／抗—ＨＢｅ附性慢性乙肝患含有１７例（占８５％）在１８９６位核苷酸发生点突变，形成终止密码，其中１０例（占５０％）发生２点突变。选突变ＰＣＲ产物直接测序，同样证实点突变的存在。提示乙肝患含ＨＢｅＡｇ阳性自然转换为抗—ＨＢｅ阳性、并不一定是病毒复制减弱，而可能足ＨＢＶＤＮＡ前Ｃ区发生了基因变异。     

乙肝患者外周血单个核细胞乙肝病毒DNA的检测及临床意义

目的：研究乙肝患者外周血单个核细胞乙肝病毒ＤＮＡ检测的临床意义。方法：用聚合酶链反应技术检测６０例乙肝患者外周血单个核细胞及血清中乙肝病毒ＤＮＡ。结果：６０例乙肝患者外周血单个核细胞及血清中乙肝病毒ＤＮＡ的阳性率分别为４３．３％，４５％；同时检测血清中ＨＢｅＡｇ，其阳性率为２８．２％；外周血单个核细胞乙肝病毒ＤＮＡ的阳性检测率与血清乙肝病毒ＤＮＡ具有一致性；急性肝炎患者外周血单个核细胞乙肝病毒ＤＮＡ的阳性率为２０％，慢性肝炎和肝炎肝硬变分别为５６．７％，４０％，外周血单个核细胞乙肝病毒ＤＮＡ的阳性检出率在急性肝炎和慢性肝炎之间有显著差异。结论：外周血单个核细胞乙肝病毒ＤＮＡ的存在与乙型肝炎的慢性化有关     

丙型肝炎病毒NS5B区基因的扩增、克隆及序列分析

目的 对丙型肝炎病毒(HCV)NSSB区基因克隆并测序，研究其变异状况。方法 从HCV RNA阳性血清中抽提病毒RNA，利用巢式RT-PCR对NS5B区基因进行扩增，将扩增获得的靶cDNA克隆至P^KPL-3a质粒载体中，并以内引物为测序引物进行序列测定分析。结果 构建了已克隆HCVNS5B区基因的重组质粒KHC5—1；序列分析表明，与HCV-BDS株同源性最高，属于主要流行于我国的HCV 1b基因亚型。结论 证实了在NS5B区，与RNA依赖的RNA聚合酶(RDRP)活性密切相关的基元结构Gly-Asp-Asp及其相邻序列的同源性非常高。     

慢性乙型肝炎患者196例病毒基因分型与临床的关系

目的探讨西安市中心医院感染科诊治的慢性乙肝患者血清中HBV基因型的分布状况及HBV基因型与临床特征的关系。方法采用巢式PCR法对196例慢性HBV患者的血清进行病毒基因型检测,并分析HBV基因型与患者临床特征的关系。结果 196例慢性HBV患者的基因分型结果为C型138例(70.4%)、B型40例(20.4%)、B/C混合型18例(9.2%),基因型的分布在性别上无统计学差异;各基因型在无症状携带者、慢性乙肝、肝硬化、肝癌中的分布差异也无统计学意义(P>0.05);C型感染者血清HBeAg阳性率(75.2%)高于B型感染者(14.2%),差异有统计学意义(P<0.05)。各基因型患者在HBV-DNA载量和ALT水平上无统计学差异(P>0.05)。结论西安市中心医院感染科诊治的HBV患者的基因型以C型和B型为主;基因C型患者血清HBeAg阳性率高于B型;基因C型患者与B型相比在肝脏炎症程度、HBV-DNA载量方面无显著差异;基因B型和C型患者临床特点相似。     

慢性乙型肝炎HBV前C区基因突变的临床研究

采用聚合酶链反应（PCR）及单链构象多态分析（SSCP）银染技术检测了32例HBV DNA阳性慢性乙型肝炎患者及乙肝病毒携带者的前C区基因变异。结果表明有前C区基因突变株感染者8例，突变株感染者检出率为25．0％；突变株的检出率以慢性乙肝重度最高，其次是慢性乙肝中度、轻度患者，乙肝病毒携带者最低；抗－HBe阳性患者前C区突变株检出率高于HBeAg阳性患者。提示HBV前C区突变与病情轻重及e系统血清标志有关。     

白细胞中乙型肝炎病毒前C区变异株的感染及动态观察

目的 :探讨白细胞中HBV前C基因变异对肝功能的损害。方法 :用特异性引物聚合酶链反应 (3’BS -PCR) ,检测 10 1例慢性乙型肝炎患者。结果 :①白细胞中变异总检出率 34.6 % ;②白细胞中存在HBV前C基因变异时血清中的谷丙转氨酶 (ALT)普遍升高。结论 :①慢性乙型肝炎患者白细胞中不仅普遍存在HBVDNA ,同时还存在HBV突变株 ;②白细胞中的变异株感染可能与发病程度有关。     

哈尔滨地区肝炎患者血清中HBV DNA基因型分析

目的 分析哈尔滨地区乙型肝炎患者HBV基因型的分布情况.方法 采用基因型特异性引物巢式聚合酶链式反应法对96例肝炎患者血清中HBV DNA基因型进行分析.结果 96例肝炎患者中92例HBV DNA检测阳性,其中80例患者属于C基因型,占87.0%.2例为B基因型,占2.2%.10例未检出型别.结论 在哈尔滨地区HBV感染同时存在B和C基因型,以C基因型为主,且可能存在其他型别或发生基因突变.     

一种新发现的乙型肝炎病毒基因变异，C基因启动子nt1726—1730聚集变异及基可能的临床意义

目的：研究乙型肝炎病毒（HBV）C基因启动子（CP）变异与无症状慢性HBV携带者（AsC）肝炎发作及慢性乙肝病情严重性的关系。方法：通过PCR及其产物直接测序,检测4例AsC、27例慢性乙肝和3例慢性重型乙肝病人血清的HBVCP和前C／C基因序列,并定量检测病人血清的HBVDNA。结果：（1）CP主要变异除核苷酸（nt）17621764双变异（1762A→和1764G→A）外,还存在nt1726－1730聚集变异（1726A→C、1727A→T、1730G→G）。（2）11例首次急性发作的慢性乙肝病人中（既往均有AsC史）,8例出现CP聚集变异。而5例隐匿起病的慢性乙肝病人和4例AsC无一例出现该变异,且1例在AsC状态时无CP变异,肝炎发作时出现CP聚集变异。（3）CP聚集变异合并CP双变异的乙肝病人,临床上或表现为重型肝炎或迅速进展为活动性肝硬变,HBVDNA水平高滴度,HBVDNA（斑点法）阳性,HBeAg/抗HBe转换。结论：CP聚集变异与AsC肝炎急性发作有关;CP聚集变异与CP双变异同时存在,使慢性乙肝病人病情加重。