三白草洗剂质量标准研究

目的：建立三白草洗剂的质量控制标准.方法：采用薄层色谱法对制剂中的三白草、苦参和黄柏进行定性鉴别,采用高效液相色谱法对制剂中的苦参碱进行含量测定.结果：薄层色谱法可鉴别出三白草、苦参和黄柏,斑点清晰,分离度好,且阴性对照无干扰;苦参碱在0.109～1.090μg/mL范围内呈良好线性关系,r=0.9999,平均回收率为98.82％,RSD=0.86％.结论：该方法操作简便易行、准确可靠、灵敏度高、重现性好,可作为三白草洗剂的质量控制方法.     

从马兜铃内酰胺探讨鱼腥草安全性

鱼腥草是常见的药食同源植物,具有数千年的食用、药用历史,现有研究表明,鱼腥草安全无毒。但由于鱼腥草含有马兜铃内酰胺成分,其安全性一直存在较多争议,而关于鱼腥草中马兜铃内酰胺毒性的探讨并不多见。从鱼腥草中马兜铃内酰胺的构效关系、量效关系、实验研究等方面对鱼腥草的安全性进行探讨,为鱼腥草的食品药品开发及应用提供参考。     

三白草中总黄酮醇提工艺的研究

目的：选择提取三白草中总黄酮的最佳方法。方法：采用紫外分光光度外法测定总黄酮含量，并且利用正交实验法对影响三白草提取效果的因素进行了研究。结果：总黄酮最佳提取工艺为以药材粉碎过20目筛后，加20倍量70％乙醇回流4h提取2次，第1次加12倍量乙醇，提取2h，第2次加8倍量乙醇，提取2h。结论：该方法可用于三白草中总黄酮的提取。     

A new dehydrogeranylgeraniol antioxidant from Saururus cernuus that inhibits intracellular reactive oxygen species (ROS)-catalyzed oxidation within HL-60 cells.

A new triene, 12,13-dehydrogeranylgeraniol (1), was isolated from the aquatic plant Saururus cernuus and its structure determined spectroscopically. Compound 1 inhibits PMA-induced peroxide-catalyzed oxidation of 2',7'-dichlorodihydrofluorescein dye (DCFH) by reactive oxygen species (ROS) within human promyelocyctic HL-60 cells.     

中药三白草的化学成分研究(Ⅰ)

目的：寻找中药三白草的活性成分。方法：三白草地上部分乙醇提取物进行色谱分离,理化数据和光谱方法鉴定结构。结果：所得化合物鉴定结构为马兜铃内酰胺ＡⅡ（Ⅰ）、胡萝卜甙（Ⅱ）、金丝桃甙（Ⅲ）、异槲皮甙（Ⅳ）、槲皮甙（Ⅴ）、槲皮素-３-Ｏ-β-Ｄ吡喃葡萄糖（１→４）-α-Ｌ吡喃鼠李糖甙（Ⅵ）,鞣花酸（Ⅶ）和柯里拉京（Ⅷ）。结论：除Ⅲ,Ⅳ和Ⅴ外,其它５个化合物均为首次从该植物中得到。     

三白草中槲皮素的薄层色谱鉴别

目的：建立三白草药材中槲皮素的提取方法,对三白草药材中槲皮素进行薄层鉴别。方法：采用硅胶G板分离三白草药材中槲皮素成分。结果：供试品色谱在与对照品色谱相应位置上显相同颜色的斑点,可用于鉴别三白草中槲皮素。结论：薄层色谱方法简便、专属性强、重现性好,可用于三白草药材的鉴别。     

三白脂素-8的抗炎作用

三白草木脂素类化合物三白脂素－8（Saucemetilignan-8)对角叉菜胶所致的大鼠急性炎症和棉球肉芽肿,均具有明显地抗炎活性。     

不同科属木通比较

目的 :正确认识不同科属木通及其异同 ,指导临床安全高效用药。方法 :不同科属木通本草学、地理分布范围和生态环境、临床、药理、毒理等方面的比较。结果 :①木通主要来源于木通科、毛茛科和马兜铃科 3科植物 ,196 3年版《中国药典》同时收录了木通科木通、毛茛科川木通和马兜铃科关木通 ,以后各版药典则将木通科木通删去 ,仅收录了川木通和关木通。②历代所用木通多为木通科木通 ,未见毒性记载 ,近代则主要为马兜铃科关木通 ,屡见毒性报道。③马兜铃科关木通含马兜铃酸和马兜铃内酰胺 ,毒性大 ,利尿作用有待确证 ,杀菌力较差 ;木通科木通不含马兜铃酸和马兜铃内酰胺 ,安全无毒 ,且有利尿杀菌作用。结论 :临床用木通科木通比用马兜铃科关木通安全。建议药典在修改版中①恢复木通科木通正品地位 ,以正视听 ,还历史本来面目 ;②马兜铃科关木通作为副品使用 ,并严格执行药典标准。     

Lignans from the roots of Saururus chinensis

Four new lignans, saucerneol F (1), saucerneol G (2), saucerneol H (3), and saucerneol I (4), were isolated from the EtOAc extract of the roots of Saururus chinensis, together with one known compound, saucerneol D (5). The structures of compounds 1-4 were elucidated by spectroscopic analysis. These compounds showed cytotoxic activities against HT-29, MCF-7, and HepG-2 cell lines.     

Two new furanoditerpenes from Saururus chinenesis and their effects on the activation of peroxisome proliferator-activated receptor gamma

Two new acyclic furanoditerpene compounds, saurufuran A (1) and B (2), were obtained from the root of Saururus chinensis, and their structures were elucidated by means of 1D and 2D NMR spectroscopic analyses. Saurufuran A (1) is effective on the activation of peroxisome proliferator-activated receptor gamma (PPARgamma) with an EC(50) value of 16.7 microM; however, saurufuran B (2), with an EC(50) value of >100 microM, weakly activated the PPARgamma.     

Anti-inflammatory, anti-angiogenic and anti-nociceptive activities of Saururus chinensis extract

Ethnopharmacolgical relevance: The aerial parts of Saururus chinensis Baill. are used for the treatment of edema and inflammatory diseases in the Oriental folk medicine.     

Hepatoprotective diastereomeric lignans from Saururus chinensis herbs

Two new diastereomeric sauchinones (2, 3), along with the known sauchinone (1), a phenylpropanoid (sarisan), and two known lignans (galbacin and saucernetin), were isolated from the n-hexane fraction of Saururus chinensis using reversed-phase HPLC. The stereostructures of the lignans, sauchinone A (2) and 1'-epi-sauchinone (3), were established by analysis of spectroscopic data. Incubation of cultured rat hepatocytes intentionally injured by CCl(4) with each of the compounds 1, 2, and 3 significantly reduced the levels of glutamic pyruvic transaminase released by the damaged hepatocytes.     

Low-density lipoprotein-antioxidant constituents of Saururus chinensis.

A new diarylbutane lignan, saururin A (1), and a known 8-O-4'-type neolignan, machilin D (2), were isolated from a total methanol extract of the underground parts of Saururus chinensis. The structures of 1 and 2 were elucidated by spectroscopic data analysis. Compounds 1, 2, and virolin (3) (the methyl ether of 2) exhibited significant low-density lipoprotein (LDL)-antioxidant activity in the thiobarbituric acid-reactive substance (TBARS) assay with IC(50) values of 8.5, 2.9, and 4.3 microM, respectively.     

鱼腥草总生物碱的大孔吸附树脂纯化工艺

目的:优选鱼腥草总生物碱大孔吸附树脂的分离纯化工艺.方法:以盐酸小檗碱、胡椒内酰胺及马兜铃内酰胺B含量为指标,比较不同型号树脂和不同工艺条件对鱼腥草总生物碱的分离纯化能力.结果:HPD-100型大孔树脂对鱼腥草总生物碱有良好的吸附分离性能,最佳工艺条件为药液质量浓度20 g?L-1,pH 3,吸附流速0.5 mL? min -1,洗脱溶剂为氨水乙醇溶液(氨水浓度0.5 mol?L-1,乙醇体积分数80％),洗脱剂用量4 BV,洗脱速度3.0mL?min -1.结论:HPD-100型大孔树脂可用于鱼腥草总生物碱的提取纯化.     

三白草中三白草酮提取工艺优选

目的:优选三白草中三白草酮的提取工艺参数.方法:采用HPLC测定三白草酮提出量,并以此为指标,正交试验法对粉碎度、乙醇体积分数、料液比、提取时间、浸泡时间5个因素进行考察,优选最佳提取工艺.结果:最佳提取条件为药材过10目筛,95％乙醇提取2次,第1次加14倍量乙醇浸泡2h,提取2h;第2次加10倍量乙醇提取1h.在此条件下,三白草酮平均提取得率0.21％,提取转移率为96％.结论:优选的提取工艺稳定、合理,可用于三白草中三白草酮的提取.     

马兜铃内酰胺-Ⅰ进入人肾小管上皮细胞及细胞内分布和蓄积的观察

目的:观察马兜铃内酰胺-Ⅰ(AL-Ⅰ)能否进入肾小管上皮细胞及其在细胞内的分布、蓄积情况。方法:以体外培养的人类近端肾小管上皮细胞系(HK-2)为研究对象,用不同质量浓度的AL-Ⅰ(5～20μg.mL^-1)处理细胞,采用荧光倒置显微镜观察细胞内由AL-Ⅰ产生荧光的有无及其分布,并在洗去含AL-Ⅰ的培养液后继续培养HK-2细胞48h,观察细胞内AL-Ⅰ荧光的持续情况以判断其蓄积性。结果:不同质量浓度的AL-Ⅰ(5～20μg.mL^-1)处理细胞0.5h内,均能在肾小管上皮细胞内观察到蓝绿色的AL-Ⅰ荧光,并且荧光仅分布于细胞浆中,而在细胞核内未观察到。在洗去含AL-Ⅰ培养液后继续培养时AL-Ⅰ的荧光可在细胞内持续存在48h以上,其持续存在部位仍为细胞浆。结论:AL-Ⅰ可以在短时间内迅速进入肾小管上皮细胞,其分布部位在细胞浆,不进入细胞核;AL-Ⅰ在细胞浆内的蓄积可能与其致肾损伤的毒性作用机制有关,即通过进入肾细胞并蓄积于细胞内在肾病过程中持续发挥毒性作用。     

三白草的化学成分研究(Ⅰ)

目的 研究三白草Saururuschinensis(Lour.)Baill.的化学成分。方法 采用硅藻土柱层析和反复硅胶柱层析分离纯化,通过理化常数测定和光谱分析鉴定其化学结构。结果 从三白草中分得了3个化合物,即三白脂素(saucernetin,Ⅰ)、三白脂素-8(saucernetin-8,Ⅱ)和三白脂素-7(saucernetin-7,Ⅲ)。结论 它们均为首次从该植物中分得的新木脂素类化合物。     

The hepatoprotective and antifibrotic effects of Saururus chinensis against carbon tetrachloride induced hepatic fibrosis in rats

Ethnopharmacological relevance

Saururus chinensis (Lour.) Baill (Saururaceae) has been used in Chinese folk medicine for treatment of various diseases, such as edema, jaundice, gonorrhea, antipyretic, diuretic, and antiinflammatory agents.

Aim of the study

Our aim was to evaluate the hepatoprotective and antifibrotic effects of Saururus chinensis extract (SC-E) in carbon tetrachloride (CCl₄) induced liver fibrosis rats.

Materials and methods

The SC-E (70 mg/kg) was administrated via gavage once a day starting from the onset of CCl₄ treatment (14 weeks) for subsequent 8 weeks. Evaluated with liver index, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid (HA), hepatic malondialdehyde (MDA) content, and superoxide dismutase (SOD) activity, total cholesterol (TC), triglyceride (TG), total lipoprotein (TP), albumin (ALB), hydroxyproline (HYP), total antioxidant capacity (T-AOC), laminin (LN), type III collagen terminal peptide (PC-IIINP), and type IV collagen (IV-C), as well as with histopathologic changes of liver.

Results

SC-E effectively reduced the elevated levels of liver index, serum ALT, AST, HA, and hepatic MDA contents, enhance the reduced hepatic SOD activity in CCl₄-treated rats. The histopathological analysis suggested that SC-E obviously alleviated the degree of liver fibrosis induced by CCl₄.

Conclusions

Those results suggest SC-E has protective and therapeutic effects on liver fibrosis induced by CCl₄.