claudin-4和MMP-2在子宫内膜癌中的表达及其意义

目的：探讨claudin-4和MMP-2在子宫内膜癌中的表达与临床病理参数之间关系。方法：采用免疫组化S—P法检测75例子宫内膜癌和28例正常增生期子宫内膜中claudin-4和MMP-2表达并作相应分析。结果：子宫内膜癌中claudin-4和MMP-2表达均高于正常增生期子宫内膜中的表达（P〈0．05）;claudin-4和MMP-2高表达均与癌组织肌层浸润深度有关（P〈0．05）,与组织学类型、组织分级、有无淋巴结转移及手术病理分期无关（P〉0．05）;claudin-4与MMP-2呈正相关（r=0．53,P〈0．05）。结论：claudin-4和MMP-2高表达可能促进子宫内膜的发生和发展,claudin-4和MMP-2表达可能是判断子宫内膜癌预后的重要参考指标。     

子宫内膜癌组织中DcR3的表达及临床意义

目的:检测DcR3在正常增生期子宫内膜、子宫内膜非典型增生和子宫内膜癌组织中的表达,探讨它们在子宫内膜癌发生、发展中的作用.方法:用免疫组织化学法检测DcR3在不同子宫内膜组织中的表达情况.结果:DcR3在子宫内膜癌组织中的表达水平均高于子宫内膜非典型增生和增生期子宫内膜组织(P<0.01).DcR3的表达与子宫内膜癌的临床分期和有无淋巴结转移有关(P<0.05),但与不同病理类型、组织学分级无关(P>0.05).结论:DcR3 在子宫内膜癌的发生、发展过程中可能起着重要的作用.     

PTEN与MMP-9在子宫内膜癌中的表达及临床意义

目的:探讨PTEN与MMP-9在子宫内膜癌中的表达、临床意义及二者之间的相关性。方法:采用免疫组织化学SP法检测40例子宫内膜腺癌,10例子宫内膜不典型增生和12例正常子宫内膜石蜡标本中PTEN及MMP-9的表达情况。结果:子宫内膜腺癌组织与子宫内膜不典型增生、正常子宫内膜组织相比,PTEN阳性表达率降低,差异有非常显著性(2χ=12.476,P<0.01);MMP-9的阳性表达率增加,差异有非常显著性(2χ=24.996,P<0.01)。PTEN阳性表达与子宫内膜腺癌病理分级及淋巴结转移有关(P<0.05),与手术病理分期无关(P>0.05)。MMP-9强阳性表达与子宫内膜腺癌病理分级及淋巴结转移相关(P<0.05),与手术病理分期显著相关(P<0.01)。在子宫内膜腺癌中,PTEN与MMP-9的表达呈负相关(r=-0.447,P<0.01)。结论:PTEN的表达缺失可能是诊断子宫内膜腺癌组织与癌前病变的分子标志物,MMP-9在子宫内膜腺癌的浸润转移中起促进作用,与子宫内膜腺癌的预后不良有关。     

联合检测LIN28B和HMGA2在子宫内膜病变中的表达及临床意义

目的:研究LIN28B和HMGA2在子宫内膜病变组织中的表达以探索子宫内膜癌的发病机制,并探讨联合检测两者在子宫内膜癌的诊断及评估中的临床价值。方法:用免疫组化法（SABC）检测正常子宫内膜36例做为对照组,非典型增生组21例,子宫内膜癌136例,共157例做为实验组,观察LIN28B和HMGA2在正常子宫内膜及不同子宫内膜病变组织中的阳性表达情况。结果:LIN28B和HMGA2在子宫内膜癌组织中的表达阳性率均较正常子宫内膜组织及非典型增生子宫内膜高(P<0.05),非典型增生子宫内膜组织中LIN28B和HMGA2的表达阳性率较正常子宫内膜组织高（P<0.05）。LIN28B的表达阳性率与手术病理分期有关,而与肌层浸润、分化程度、淋巴转移及患者绝经与否无关（P>0.05）。HMGA2的表达阳性率与手术病理分期有关（P<0.05）,可能与肌层浸润有关（P=0.0496）,而与分化程度、淋巴转移及患者绝经与否无关（P>0.05）。136例子宫内膜癌组织中LIN28B和HMGA2表达呈正相关（r=0.327,P<0.05）。结论:LIN28B和HMGA2与子宫内膜组织的异常增生及癌变密切相关。LIN28B可能通过某种途径上调HMGA2的表达,从而诱导子宫内膜癌的发生与发展。两者可能成为对于子宫内膜癌诊断及评估有一定参考价值的检测指标。     

人附睾蛋白4在子宫内膜癌组织及血清中的表达及临床意义

目的:探讨人附睾蛋白4(HE4)在子宫内膜癌组织及血清中的表达及临床意义.方法:选取2009年9月至2010年3月宁夏医科大学附属31例子宫内膜癌患资料,用免疫组织化学法检测正常对照组(正常增生期子宫内膜)20例,子宫内膜增生组19例及子宫内膜癌组31例组织中HE4蛋白的表达水平,检测结果用image pro-plus 6.0专业图像分析软件进行定量分析,选择测定目标积分光密度(IOD)作为评价参数.应用酶联免疫吸附试验检测正常对照组(正常增生期子宫内膜)20例,子宫内膜增生组19例及子宫内膜癌组31例患者血清中HE4的水平.结果:1)HE4在子宫内膜癌组的表达高于正常对照组及子宫内膜增生组,差异有统计学意义(P<0.001).HE4在子宫内膜癌Ⅰ期组织中的IOD中位数低于Ⅱ、Ⅲ期组织中IOD中位数,差异有统计学意义(P<0.001).HE4在子宫内膜癌组织中IOD与FIGO分期呈正相关关系(P<0.001).2)子宫内膜癌组HE4的血清水平均高于正常对照组及子宫内膜增生组,差异有统计学意义(P<0.05);子宫内膜癌组术后血清中HE4的表达水平明显低于术前(P<0.05);3)子宫内膜癌组组织中的表达和术前血清中的水平呈正相关关系;4)子宫内膜癌组术前血清HE4水平与FIGO分期、血清CA125呈正相关关系.结论:子宫内膜癌组织及血清HE4表达升高,可作为子宫内膜癌血清学标志物,是子宫内膜癌的早期辅助诊断、疗效评价、预后评估的客观指标,可用于指导临床的综合治疗.     

葡萄糖转运蛋白1和磷脂结合蛋白-1在子宫内膜癌中的表达及意义

背景与目的:探讨葡萄糖转运蛋白1(GLUT1)和磷脂结合蛋白.1(Annexin-1)在子宫内膜癌组织中的表达情况及其与临床病理参数之间的关系.材料与方法:采用免疫组化S-P法检测65例子宫内膜癌、27例非典型增生和21例增生期子宫内膜组织中GLUT1和Annexin-1的表达.结果:在增生期子宫内膜、非典型增生、子宫内膜癌的GLUT1阳性表达率分别为28.6%、59.3%、81.5%,呈递增趋势,组间两两比较,差异均具有统计学意义(P<0.05);Annexin-1阳性表达率分别为85.7%、55.6%、49.2%,呈下降趋势,其中子宫内膜癌与增生期子宫内膜比较差异有统计学意义(P<0.05).GLUT1高表达与子宫内膜癌的组织分级、肌层浸润深度有关(P<0.05),与病理分期、淋巴结是否转移、组织学类型无关(P>0.05);Annexin-1低表达与上述的临床病理参数皆无关(P>0.05).子官内膜癌中GLUT1与Annexin-1呈负性相关(r=-0.540,P=0.000).结论:Annexin-1低表达和GLUT1高表达可能对子宫内膜癌的发牛和发展具有促进作用,二者对子宫内膜癌早期诊断和预后预测有一定意义.     

Claudin-4和PTEN在子宫内膜癌中的表达及其意义

 目的 探讨claudin-4和PTEN在子宫内膜癌中的表达、意义及两者之间的关系。 方法 采用免疫组化SP法检测75例子宫内膜癌、35例不典型增生和28例增生期子宫内膜的claudin-4和PTEN表达。 结果 从正常增生期子宫内膜、非典型增生、到宫内膜癌,claudin-4阳性表达呈逐渐增高趋势,组间差异有统计学意义（P<0.05）;PTEN阳性表达呈下降趋势,组间差异有统计学意义（P<0.05）。claudin 4高表达与癌组织肌层浸润有关（P<0.05）,与组织分级、手术病理分期、组织学类型及有无淋巴结转移无关;PTEN低表达与肌层浸润有关（P<0.05）,与组织分级、手术病理分期、组织学类型、及有无淋巴结转移无关。claudin 4与PTEN呈负相关（r= 0.36, P<0.05）。 结论 claudin-4高表达可能促进了子宫内膜癌发生和发展,PTEN低表达在这一过程中可能具有协同作用。     

DANCR在不同子宫内膜病变组织中的表达及意义

目的:探讨在子宫内膜癌和子宫内膜非典型增生及正常子宫内膜组织中DANCR的表达及意义。方法:收集2016年手术切除的子宫内膜癌组织55例、正常子宫内膜标本30例以及子宫内膜非典型增生组织7例,运用实时PCR技术分别检测DANCR在不同子宫内膜病变组织中的表达情况,并分析其与临床病理参数以及预后的关系。结果:子宫内膜癌组织中DANCR的表达水平高于子宫内膜非典型增生及正常子宫内膜组织,3组间差异有统计学意义（P<0.05）,并且在正常子宫内膜组织、子宫内膜非典型增生以及子宫内膜癌中呈逐渐上升趋势;DANCR在I型子宫内膜癌中的表达高于II型子宫内膜癌,差异具有统计学意义（P<0.05）;结论:DANCR在子宫内膜癌中显著性高表达,可能与子宫内膜癌的发展有关;DANCR可能对子宫内膜癌病理分型有标志性的意义。     

JMJD2A在子宫内膜癌中的表达及意义

目的:探讨组蛋白去甲基化酶JMJD家族中的JMJD2A在正常子宫内膜以及子宫内膜癌中的表达及临床意义.方法:收集临床手术标本,包括子宫内膜癌组织56例,正常子宫内膜组织20例.采用免疫组化的ElⅣisionTM法检测标本中JMJD2A抗体的表达,分析其表达水平与临床病理参数之间的关系,探讨其临床意义.结果:JMJD2A在子宫内膜癌组织中的表达高于正常子宫内膜组织(P<0.05),JMJD2A的表达与年龄、子宫内膜癌的组织学分级和临床病理分期无相关(P>0.05).结论:JMJD2A可能是一个与子宫内膜癌相关的癌基因,高表达提示子宫内膜癌.     

高迁移率族蛋白A2在子宫内膜癌中的表达及意义

目的:探讨高迁移率族蛋白A2(high mobility group protein A2,HMGA2)在子宫内膜癌细胞系AN3CA、ECC-1、Ishikawa以及子宫内膜癌组织、正常子宫内膜组织中的表达及意义.方法:采用逆转录-多聚合酶链反应(RT-PCR)、免疫印迹法(Western Blot)检测HMGA2在体外培养的子宫内膜癌细胞系AN3CA、ECC-1、Ishikawa以及21例子宫内膜癌组织标本和18例正常子宫内膜组织标本中的表达.结果:HMGA2-mRNA在子宫内膜癌细胞系AN3CA、ECC-1、Ishikawa中均有表达;在I期、Ⅱ期子宫内膜癌患者中表达的阳性率分别为8.3%(1/12)、88.9%(8/9),HMGA2-mRNA表达的阳性率与手术-病理分期有关(P=0.000,P<0.05),在高分化腺癌、中低分化腺癌的组织标本中表达阳性率分别为66.7%(4/6)、33.3%(5/15),HMGA2-mRNA表达阳性率与子宫内膜癌细胞的分化程度无关(P=0.331,P>0.05);HMGA2-mRNA在18例正常子宫内膜组织标本中无一例表达;Western Blot的结果与RT-PCR的结果相符合.结论:HMGA2基因可能参与了子宫内膜癌的发生和发展,在子宫内膜癌细胞的增殖与侵袭中可能发挥重要作用.     

Bacteria and cancer--antagonisms and benefits

There is considerable historical and recent evidence concerning the antagonisms between acute bacterial infections or their toxins and cancer and allied diseases. These data provide renewed incentives to undertake clinical programmes with mixed bacterial vaccines in many countries at the present time.     

Religiosity and physical and emotional functioning among African American and White colorectal and lung cancer patients

The literature suggests that religiosity helps cope with illness. The present study examined the role of religiosity in functioning among African Americans and Whites with a cancer diagnosis. Patients were recruited from an existing study and mailed a religiosity survey. Participants (N = 269; 36% African American, 56% women) completed the mail survey, and interview data from the larger cohort was utilized in the analysis. Multivariate analyses indicated that in the overall sample religious behaviors were marginally and positively associated with mental health and negatively with depressive symptoms. Among women, religious behaviors were positively associated with mental health and negatively with depressive symptoms. Religiosity was not a predictor of study outcomes for men. Among African Americans, religious behaviors were positively associated with mental health and vitality. Among Whites, religious behaviors were negatively associated with depressive symptoms. These findings suggest a mixed role of religious involvement in cancer outcomes. The current findings may have applied potential in the areas of emotional functioning and depression.     

Renal irradiation and the pharmacology and toxicity of methotrexate and cisplatinum

We used a rat model to study the effects of renal irradiation on the pharmacology of methotrexate (MTX) and cisplatinum (cis-Pt). Unanesthetized rats were given bilateral kidney irradiation (20 Gy in 9 fractions). At 9 months after irradiation, 3% of the animals had died and survivors showed moderately impaired renal function. At 15 months, 30% of the animals had died and survivors showed severely impaired renal function. Some animals were given i.v. MTX 1 week to 15 months after irradiation. In irradiated rats, the area under the MTX plasma clearance curve equaled that of controls through 6 months, and was significantly above controls from 9 months on. Other animals were given i.p. cis-Pt 1 week to 9 months after irradiation. The acute toxicity of cis-Pt was the same in control and irradiated rats when cis-Pt was given immediately before or after irradiation. Beginning 3 months after irradiation there was a progressive increase in cis-Pt toxicity and a simultaneous decrease in urinary platinum excretion. Irradiated animals that survived cis-Pt treatment showed increased radiation nephritis; the greatest effect occurred when cis-Pt was given 3 months or more after irradiation. MTX and cis-Pt clearance decreased when renal dysfunction was first observed and changes in renal function preceded changes in drug clearance and toxicity.     

VEGF及KDR在大肠腺瘤和腺癌中的表达及意义

目的：探讨VEGF和KDR在大肠腺瘤和大肠腺癌中的表达及临床病理特征的关系。方法：大肠腺瘤和大肠腺癌组织标本各100例,采用免疫组织化学染色法检测VEGF和KDR在标本中的表达情况。结果：VEGF和KDR在大肠腺癌组中的阳性表达明显高于大肠腺瘤组（P〈0.05）;在正常大肠黏膜均未见VEGF和KDR表达的阳性染色;VEGF阳性表达组中KDR的阳性表达率为70%,显著高于VEGF阴性表达组中KDR的阳性表达率16%,两组比较有统计学意义（P〈0.01）。结论：大肠腺癌组织中KDR的表达与肿瘤大小、转移情况、浸润深度密切相关;VEGF和KDR在大肠腺瘤中的表达与患者的年龄、性别及分型均无相关性,而与增生程度相关（P〈0.05）。在大肠腺癌患者中VEGF及KDR表达更高,二者具有协同效应。     

Morphine and tumor growth and metastasis

Morphine is an analgesic widely used to alleviate cancer pain. In addition, the perioperative management of pain in cancer surgery patients most often includes opioids. However, there are reports that these drugs may alter cancer recurrence or metastasis. Several mechanisms have been proposed, such as the modulation of the immune response or cellular pathways that control the survival and migratory behavior of cancer cells. The published literature, however, presents some discrepancies, with reports suggesting that opioids may either promote or prevent the spread of cancer. It is of great importance to determine whether opioids, in particular the most widely used, morphine, may increase the risk of metastasis when used in cancer surgery. This review examines the available data on the effects of morphine which influence cancer metastasis or recurrence, including immunomodulation, tumor cell aggressiveness, and angiogenesis, with special emphasis on recently published clinical and laboratory based studies. We further discuss the parameters that may explain the difference between reports on the effects of morphine on cancer.     

肾上腺素能β受体与肿瘤生长及转移

大量研究表明肿瘤细胞可表达β受体，而一些神经递质、药物和社会心理因素可能通过β受体影响肿瘤的生长和转移，β受体激动剂、β受体阻滞剂以及抑郁等社会心理因素可加强或削弱这种作用。这为表达β受体肿瘤的治疗开辟了新的道路，提供了新的治疗靶点。