共查询到20条相似文献,搜索用时 109 毫秒
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中药指纹图谱是指中药经适当处理后,采用一定的分析手段,得到的能够标示其特性的共有峰的图谱。中药指纹图谱是一种综合的、可量化的化学(不包括生物学)鉴定手段,可以鉴别真伪,评价原料药材、半成品和成品质量的均一性和稳定性[1]。利用高效液相色谱-质谱(HPLC-MS)联用技术,将HPLC对复杂样品的高分离能力与MS的高选择性、高灵敏度以及能够提供分子量与结构信息的优点结合起来,可对中药质量进行比较全面的评价[2]。本文就HPLC-MS联用技术的发展近况以及近年来该技术在中药材和中药制剂指纹图谱研究中的应用作一综述。1HPLC-MS联用… 相似文献
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色谱指纹图谱在中药产品专利申请中的应用 总被引:1,自引:0,他引:1
中药产品的专利申请是保护中药知识产权,加强中药创新研究的有效方式之一。本文论述了中药指纹图谱在专利申请中的特点,认为结合现代分析工具——色谱指纹图谱技术进行专利申请是一条有效显示专利创造性和新颖性的途径。 相似文献
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色谱指纹图谱在中药质量标准体系中的应用 总被引:2,自引:0,他引:2
该文综述了色谱指纹图谱在中药质量标准体系中的应用。介绍了我国中药质量标准体系的现状,色谱指纹图谱在中药质量标准体系中的重要性,以及色谱指纹图谱的定义和特点,并详细介绍的了薄层色谱指纹图谱、高效液相色谱指纹图谱、气相色谱指纹图谱、高效液相色谱指纹图谱和色谱联用技术在中药质量标准体系中的应用。 相似文献
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指纹图谱在中药质量控制中的应用进展 总被引:5,自引:1,他引:4
本文综述了化学指纹图谱、生物指纹图谱及代谢指纹图谱在中药质量控制研究中的应用。化学指纹图谱已经发展比较成熟,并广泛应用于中药及其制剂的定性、定量研究中;生物指纹图谱和代谢指纹图谱与中药的药效活性相关,其发展尚处于起步阶段,但已经越来越受到人们的重视;建立从原料、中间体到制剂的包括化学与药效学信息的多维多息指纹图谱已经成为解决中药目前"信任危机"最有效的手段。 相似文献
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现代色谱技术在中药指纹图谱研究中的应用 总被引:1,自引:0,他引:1
中药指纹图谱的研究必须与现代分析技术相结合。本文以国内近年来发表的论文为基础,论述了色谱技术在中药指纹图谱研究中的的应用现状。指出应灵活运用不同的分析手段,依靠计算机技术,进行中药指纹图谱的构建,从而实现中药材及中成药的质量控制,推进中药的现代化进程。 相似文献
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色谱指纹图谱在中药质量控制中的应用研究 总被引:1,自引:0,他引:1
通过对中药材质量控制的重要性以及中药自身特点的概述,说明要实现中药现代化,采用适当的分析手段进行中药指纹图谱研究是非常必要的;同时对中药指纹图谱以及在中药质量控制中的应用研究进行了探讨,并对中药指纹图谱的应用前景进行了展望。 相似文献
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Gammelgaard B Hansen HR Stürup S Møller C 《Expert opinion on drug metabolism & toxicology》2008,4(9):1187-1207
Background: The inherent properties of element selectivity combined with high sensitivity and structure independent response, make inductively coupled plasma mass spectrometry (ICP-MS) an interesting alternative detection technique in drug metabolism studies. Objective: The application of online separation with ICP-MS detection in drug metabolism studies is reviewed with focus on the merits and demerits of this detection technique. The prerequisite for inclusion in this review is that the study involves a separation technique hyphenated online to the ICP-MS detection. Result/conclusion: ICP-MS detection is found to be advantageous for analysis of all drug substances detectable by ICP-MS compared to radiochemical detection. Detectable drugs are limited to halogen-, sulfur-, metal- and metalloid-containing compounds. The drawback of interference from endogenous compounds on quantitative mass balance estimations of non-metal drugs is addressed. The potential of determining the stoichiometry in metallo-drug biomolecule interactions is pointed out by presenting examples of simultaneous monitoring of metals in metallo-drugs and intrinsic ICP-MS detectable elements in biomolecules. It is concluded that ICP-MS detection is an indispensable technique in drug metabolism studies of metallo-drugs, although the applicability for traditional drugs is limited. 相似文献
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基于主成分分析的中药色谱指纹图谱多维多息特征数据挖掘方法研究 总被引:3,自引:1,他引:3
目的对评价中药色谱指纹图谱多维多息的F和I等37个特征参数进行数据挖掘,为用计算机解析与评价图谱、建立标准的指纹图谱提供理论参考和实践探索。方法运用统计软件SPSS对10批次不同产地的当归指纹图谱的37个多维多息特征参数进行主成分分析。结果运用药学专业知识对分析过程产生的4个主成分进行命名,计算主成分得分,其中S10批次样本得分最高为1.52。结论综合主成分得分排名可作为评价指纹图谱的模型,从对各主成分的命名中发现了4个主成分能够反映中药色谱指纹图谱信息的规律,证实了主成分分析能达到降维目的,使繁多的求解目标简化,可用于中药指纹图谱的数据挖掘。 相似文献
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An approach was proposed to develop binary chromatographic fingerprints by means of high performance liquid chromatography/diode array detector (HPLC/DAD) and gas chromatography/mass spectrometry (GC/MS). HPLC fingerprint and GC/MS fingerprint that, respectively, represent chemical characteristics of aporphinoid and quinolizidine alkaloids of the total alkaloids from Caulophyllum robustum were developed, which were used to construct binary chromatographic fingerprints of the total alkaloids. Moreover, the authentication and validation of the binary fingerprints were performed. Then, a data-level information fusion method was employed to capture the chemical information encoded in two chromatographic fingerprints. Finally, based on the fusion results, the quality of 10 batches of the total alkaloids samples was further evaluated by similarity measure and cluster analysis method. In comparison with conventional fingerprint, the binary chromatographic fingerprints which represent the characteristics of more constitutions can comprehensively and properly reveal the quality characteristics of the total alkaloids. The binary chromatographic fingerprints can reach more objective conclusions in the practice of quality control of the total alkaloids. In conclusion, the binary chromatographic fingerprints are suitable for quality control of the total alkaloids. The presented approach is a powerful and meaningful tool to comprehensively conduct the quality control of traditional Chinese medicine (TCM). 相似文献
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The application of inductively coupled plasma mass spectrometry in pharmaceutical and biomedical analysis 总被引:2,自引:0,他引:2
Huang J Hu X Zhang J Li K Yan Y Xu X 《Journal of pharmaceutical and biomedical analysis》2006,40(2):227-234
With the development of life science, pharmaceutical and biomedical analysis becomes more and more important in medical science. Further studies will be hopefully established if it is possible to use inorganic elemental standards or small organic compounds in the quantitative determination of all kinds of drugs, nucleotides and sulfur or phosphorus containing peptides and proteins at appropriate concentration with an acceptable accuracy. Since 1980, inductively coupled plasma mass spectrometry (ICP-MS) has emerged as a new and powerful analytical technique which is suitable for element and isotope analysis. It offers extremely wide detection range of element and co-analysis of most elements in the periodic table. Also, it can be applied to perform qualitative, semiquantitative, and quantitative analysis and isotopic ratios through mass-to-electric charge ratio. With the help of ICP-MS, the struggle of searching for an excellent quantification technique in, e.g. drugs and proteomics has come appreciably close to an end. This review mainly focuses on the introduction of application of ICP-MS in pharmaceutical and biomedical analysis. Some problems in application and the handling strategies are simply presented at the end. 相似文献
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目的探讨脉搏碳氧血红蛋白仪快速测定碳氧血红蛋白饱和度(HbCO%)在院前对一氧化碳中毒患者及早诊断的价值。方法2008年10月~2009年3月收集54例CO中毒患者,按照临床症状和体征和患者病情严重程度分为轻、中、重3组,对总体和各组具体病情恢复情况进行具体的分析。结果轻、中度两组HbE0%差异无统计学意义(P=0.384),轻、重度间比较差异有高度统计学意义(P〈0.01),中、重度间EE较差异有高度统计学意义(P〈0.01)。结论脉搏碳氧血红蛋白定量检测仪对一氧化碳中毒患者的院前急救有很大的诊断价值。 相似文献
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生物质谱技术在蛋白质结构鉴定中的应用进展 总被引:3,自引:1,他引:3
综述了生物质谱技术在蛋白质结构鉴定中的应用新进展,讨论了生物质谱在蛋白质初步鉴定、翻译后修饰的鉴别、蛋白质-蛋白质间相互作用等研究方面的应用趋势,以及一些最新发展,指出了生物质谱技术现存的主要问题,探讨了可能的解决方案。 相似文献
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Techniques: the application of accelerator mass spectrometry to pharmacology and toxicology 总被引:1,自引:0,他引:1
The exquisite sensitivity of accelerator mass spectrometry (AMS) is being used in biomedical applications to quantitate many isotopes, including 14C, 3H, 41Ca and 27Al, at attomole (10(-18)) concentrations. This enables compounds and metabolites to be measured in human urine and plasma after administration of low pharmacologically or toxicologically relevant doses of labelled chemicals and drugs. The detection of modified proteins or DNA in target organs after dosing with potential carcinogens has also been achieved in many studies. Advances aimed at increasing sample throughput and expanding applications by coupling AMS instruments directly to chromatographic separation systems are currently underway. 相似文献