PRE-EXPOSURE OF MICE TO LOW DOSE OR LOW DOSE RATE IONIZING RADIATION REDUCES

ＰＲＥ－ＥＸＰＯＳＵＲＥＯＦＭＩＣＥＴＯＬＯＷＤＯＳＥＯＲＬＯＷＤＯＳＥＲＡＴＥＩＯＮＩＺＩＮＧＲＡＤＩＡＴＩＯＮＲＥＤＵＣＥＳＣＨＲＯＭＯＳＯＭＥＡＢＥＲＲＡＴＩＯＮＳＩＮＤＵＣＥＤＢＹＳＵＢＳＥＱＵＥＮＴＥＸＰＯＳＵＲＥＴＯＨＩＧＨＤＯＳＥＯＦＲ...     

DIFFERENTIATION OF PSEUDOCONDYLOMA OF VULVA AND CONDYLOMA ACUMINATA BY DOT BLOT HYBRIDIZATION AND POLYMERASE CHAIN REACTION

ＤＩＦＦＥＲＥＮＴＩＡＴＩＯＮＯＦＰＳＥＵＤＯＣＯＮＤＹＬＯＭＡＯＦＶＵＬＶＡＡＮＤＣＯＮＤＹＬＯＭＡＡＣＵＭＩＮＡＴＡＢＹＤＯＴＢＬＯＴＨＹＢＲＩＤＩＺＡＴＩＯＮＡＮＤＰＯＬＹＭＥＲＡＳＥＣＨＡＩＮＲＥＡＣＴＩＯＮＬｉｕＹｕｅｈｕａ刘跃华；Ｗａｎｇ...     

INTEGRATION OF TRADITIONAL AND MODERN METHODS IN THE IDENTIFICATION OF AFB CULTURES ISOLATED FROM CLINICAL SPECIMENS OF PATIE

ＩＮＴＥＧＲＡＴＩＯＮＯＦＴＲＡＤＩＴＩＯＮＡＬＡＮＤＭＯＤＥＲＮＭＥＴＨＯＤＳＩＮＴＨＥＩＤＥＮＴＩＦＩＣＡＴＩＯＮＯＦＡＦＢＣＵＬＴＵＲＥＳＩＳＯＬＡＴＥＤＦＲＯＭＣＬＩＮＩＣＡＬＳＰＥＣＩＭＥＮＳＯＦＰＡＴＩＥＮＴＳＷＩＴＨＳＫＩＮＤＩＳＥＡＳ...     

LIGH T MICROSCOPE STUDIES OF THE MORPHOLOGY AND DISTRIBUTION OFAUERBODIESINACUTEPROMYELOCYTICLEUKEMIA

ＬＩＧＨＴＭＩＣＲＯＳＣＯＰＥＳＴＵＤＩＥＳＯＦＴＨＥ·ＭＯＲＰＨＯＬＯＧＹＡＮＤＤＩＳＴＲＩＢＵＴＩＯＮＯＦＡＵＥＲＢＯＤＩＥＳＩＮＡＣＵＴＥＰＲＯＭＹＥＬＯＣＹＴＩＣＬＥＵＫＥＭＩＡＷａｎｇＫｅｑｉａｎｇ（王克强），ＬｉＹａｎ（李雁），Ｈｕａｎｇ...     

FUNCTIONAL AND STRUCTURAL RELATION BETWEEN CENTRAL CHEMORECEPTION OF RESPIRATION AND THE NUCLEUS RETROFACIALIS IN RATS

ＦＵＮＣＴＩＯＮＡＬＡＮＤＳＴＲＵＣＴＵＲＡＬＲＥＬＡＴＩＯＮＢＥＴＷＥＥＮＣＥＮＴＲＡＬＣＨＥＭＯＲＥＣＥＰＴＩＯＮＯＦＲＥＳＰＩＲＡＴＩＯＮＡＮＤＴＨＥ　ＮＵＣＬＥＵＳＲＥＴＲＯＦＡＣＩＡＬＩＳＩＮＲＡＴＳＴｉａｎＧｏｕｆｅｎｇ；（田国锋）Ｑｉａ...     

CHANGES OF PLASMA DYNORPHIN LEVELS BEFORE AND AFTER PERCUTANEOUS BALLOON MITRAL COMMISSUROTOMY IN PATIENTS WITH MITRAL STENOS

ＣＨＡＮＧＥＳＯＦＰＬＡＳＭＡＤＹＮＯＲＰＨＩＮＬＥＶＥＬＳＢＥＦＯＲＥＡＮＤＡＦＴＥＲＰＥＲＣＵＴＡＮＥＯＵＳＢＡＬＬＯＯＮＭＩＴＲＡＬＣＯＭＭＩＳＳＵＲＯＴＯＭＹＩＮＰＡＴＩＥＮＴＳＷＩＴＨＭＩＴＲＡＬＳＴＥＮＯＳＩＳＹｉｎＲｕｉｘｉｎｇ；（尹瑞...     

A RAPID ESTIMATION OF CELL CYCLE PARAMETERS：ONE HOUR COUNTINUOUS BROMODEOXYURIDINE（BrdUrd）LABELING METHOD

ＡＲＡＰＩＤＥＳＴＩＭＡＴＩＯＮＯＦＣＥＬＬＣＹＣＬＥＰＡＲＡＭＥＴＥＲＳ：ＯＮＥＨＯＵＲＣＯＵＮＴＩＮＵＯＵＳＢＲＯＭＯＤＥＯＸＹＵＲＩＤＩＮＥ（ＢｒｄＵｒｄ）ＬＡＢＥＬＩＮＧ　ＭＥＴＨＯＤＳｈｉＸｕｅｇｅｎｇ（石学耕）（ＤｅｐａｒｔｍｅｎｔｏｆＢ...     

THE RELATIONSHIPS BETWEEN ERYTHROBLAST DENUCLEATION AND THE NUCLEAR MATRIX－INTERMEDIATE FILAMENTS

ＴＨＥＲＥＬＡＴＩＯＮＳＨＩＰＳＢＥＴＷＥＥＮＥＲＹＴＨＲＯＢＬＡＳＴＤＥＮＵＣＬＥＡＴＩＯＮＡＮＤＴＨＥＮＵＣＬＥＡＲ　ＭＡＴＲＩＸ－ＩＮＴＥＲＭＥＤＩＡＴＥＦＩＬＡＭＥＮＴＳＭａＷｅｎｌｉ（马文丽）ａｎｄＸｕｅＳｈｅｐｕ（薛社普）；（Ｄｅｐａｒｔ...     

IMPLANTATION OF AUTOLOGOUS SKIN FIBROBLAST GENETICALLY MODIFIED TO SECRETE CLOTTING FACTOR IX PARTIALLY CORRECTS THE HEMORRHA

ＩＭＰＬＡＮＴＡＴＩＯＮＯＦＡＵＴＯＬＯＧＯＵＳＳＫＩＮＦＩＢＲＯＢＬＡＳＴＧＥＮＥＴＩＣＡＬＬＹＭＯＤＩＦＩＥＤＴＯＳＥＣＲＥＴＥＣＬＯＴＴＩＮＧＦＡＣＴＯＲＩＸＰＡＲＴＩＡＬＬＹＣＯＲＲＥＣＴＳＴＨＥＨＥＭＯＲＲＨＡＧＩＣＴＥＮＤＥＮＣＩＥＳＩＮ...     

SURGICAL TREATMENT OF HALLUX VALGLUS BY RECONSTRUCTION OF METATARSAL ARCH AND MODIFIED MCBRIDE OPERATION （40 CASES REPORT）

ＳＵＲＧＩＣＡＬＴＲＥＡＴＭＥＮＴＯＦＨＡＬＬＵＸＶＡＬＧＬＵＳＢＹＲＥＣＯＮＳＴＲＵＣＴＩＯＮＯＦＭＥＴＡＴＡＲＳＡＬＡＲＣＨＡＮＤＭＯＤＩＦＩＥＤＭＣＢＲＩＤＥＯＰＥＲＡＴＩＯＮ（４０ＣＡＳＥＳＲＥＰＯＲＴ）ＷｅｎｇＸｉｓｈｅｎｇ（翁习生）；Ｈｅ...     

Effects of combined radiation and thermal burn injury on the survival of skin allograft and immune function in rats

OBJECTIVES: To investigate the effects of combined radiation and thermal burn injury on the survival of skin allografts and to analyze the relationship between the prolongation of allograft survival and the changes of immune functions of the thymocytes and splenocytes in rats. METHODS: Wistar rats were irradiated with 3, 4, 5, 6 and 8 Gy of gamma rays. Thirty minutes after radiation, 15% TBSA III-degree burn was inflicted to the rats. Twenty-four hours after the burn injury, allografts were used to cover the burn wounds. In the 8 Gy group, 1 hour before skin grafting, the bone marrow cells (4 x 10(8)) from the same donor were also transplanted. All rats were carefully observed after injury. The rats with single radiation injury of 5 Gy gamma rays, with single burn injury and with combined radiation-burn injury were killed 3, 7, 10, 15 and 30 days after skin grafting for immunological assay and pathological study. RESULTS: All the allografts in the single burn group were rejected in 10 days. In the combined injury groups, the survival rates of the allografts in rats undergoing 3 and 4 Gy radiation were 20% and 30%, respectively. In the combined injury groups undergoing 5, 6 and 8 Gy radiation, the 10-day survival rates of the allografts were 69%, 88% and 100% respectively, and the 30-day survival rates in the three groups were 36%, 42% and 100% separately. The grafted allogenic skin, with normal epithelial cells and good vascularity, healed well with the recipient's skin. Hairs grew well from the allografts 30 days after grafting. Three, 7 and 15 days after allografting, in the single burn group, the proliferative activities of the thymocytes were 90%, 185% and 130% of the preinjury level, and the antibody forming capacities of the splenocytes were 200%, 171% and 300% of the preinjury level, respectively; in the combined injury groups, the proliferative activities were 6%, 99% and 91% of the preinjury level, and the forming capacities were 2%, 36% and 90% of the preinjury level. CONCLUSIONS: The survival rate of allograft in rats undergoing combined radiation and thermal burn injury rises with the increase in radiation dosage. The allograft covering single bun injury is severely rejected by immune reaction. The prolongation of the survival of allograft in combined injury group mainly results from radiation that suppresses immune functions.     

放射损伤,烧伤与放烧复合伤早期在体心脏功能变化的实验研究

采用复合伤与单一伤相比较，研究了放烧复合伤后８ｈ以内在体心肌力学变化，并通过对伤后补液与加维拉帕米的比较，对复合伤后心肌力学变化的进行了初步的探讨。     

A study on mitochondrial respiratory dysfunction and lipid peroxidation in the liver after radiation,burn,and combined radiation-burn injuries in mice

Male mice were subjected to 6 Gy total body irradiation,20% TBSAfull-thickness burns,or combined radiation-burn injury and lipid peroxides(LPO),vita-min E,sulfhydryl group,respiratory control ratio(RCR),ADP/O ratio,and cytochromeoxidase activity of the liver mitochondria were determined in the first 9 d postinjury.Theresults are as follows:(1)LPO level increased in the early postinjury stage after combinedradiation-burn injury,on the 5th-7th day after irradiation and on the 7th day postburn.(2)Vitamin E level decreased significantly in the two groups of radiation and burn inju-ries but showed no significant decrease after combined injury.(3)The sulfhydryl groupshowed a tendency to increase in all the 3 groups.(4)The activity of cytochrome oxidaseincreased significantly on the 7th day after radiation but decreased considerably in theburn and combined injury groups.(5)RCR and ADP/O ratio decreased more significantlyin the combined injury group than in either the radiation group or the burn group.These facts suggest that the respiratory dysfunction of the liver mitochondria results mostprobably from the damage on the mitochondrial membrane due to lipid peroxidation.     

放烧复合伤与单纯放射或烧伤对大鼠在体心室肌细胞动作电位影响的比较

本文用健康SD大鼠57只,随机分为6组,用悬浮微电极记录并比较了单纯~(50)Co照射或烧伤与二者结合的放烧复合伤对在体心室肌细胞动作电位的影响,同时观察了复合伤对心肌影响的恢复过程。结果表明:放烧复合伤与单纯放射或烧伤相似.明显地影响心室肌细胞的除极化和复极化过程。但有其特点:与对照组相比,复合伤后24h,动作电位幅度降低13.6％,最大除极速率减慢41.2％。各组的心率有所不同:烧伤组增加9.8％,放射组减少17.4％,复合伤后24h组却近于对照水平,但复合伤后3天组心率减少12％,伤后5天组才略有恢复。复合伤降低平均复极化速率(-13.2％～-26.3％)及延长动作电位持续时间(APD),尤其是APD50及APD90更为明显(分别为+22.9％～+33.7％和+6.0％～+18％)。实验结果表明,放烧复合伤对大鼠心室肌动作电位的影响并非单纯放射或烧伤两种致伤作用的简单相加,在有的方面(如最大除极速率)二者是协同作用;而在另外方面(如心率)二者又是互相抵消。本实验中复合伤的致伤作用是可逆的。一般说,损伤反应出现早的参数,恢复较快,反之较慢。     

Dynamic changes of myocardial nitric oxide formation and cGMP content in the early stage after radiation, burn and combined radiation-burn injuries in rats

Nitricchide(NO)ispresentinmanyhssuesandactSasap~crineautocoidandintracellularchemicalmessenger.NOiss}vithesizedfromL--argininebynitricoxidesynthase(NOS).TwOmaintYPesofNOShavebeenidentified:aconstitutiveCab -calmedulindependentNOS(cNOS)andaCab independentNOS(iNOS)whichareexpressedafterthestimulationofendotoxinandceTtaincytoki...[IJ.RecentstudiesdemonstratedthatNOalightplayaroleinthemaculationofmyocdriialcontractility.TheactivityofbathcNOSandiNOSisidentifiedinisolatedeardialmyocy…     

原位杂交技术测定放烧复合伤创面aFGF mRNA的表达

目的 :探讨放烧复合伤创面愈合过程中aFGF的作用。方法 :用SD大鼠制备深Ⅱ°烧伤、放烧复合伤模型 ,分别于模型后 12h和 1、3、7d取背部创面皮肤组织 ,采用原位杂交技术测aFGFmRNA的表达。结果 :烧伤组大鼠在上述 4个时相点aFGFmRNA表达明显增加 ,而放烧复合伤组仅在第 7天有少量表达 ;aFGFmRNA的表达部位位于真皮及皮下。结论 :提示aFGF有助于创伤的愈合 ;放烧复合伤创面愈合延迟与aFGFmRNA表达低下及延迟有关     

放射损伤,烧伤和放烧复合伤心肌线粒体脂质过氧化的加强效应

Ｗｉｓｔａｒ大鼠经６Ｇｙ＾６０Ｃｏ γ线和／或３０％总体表面积（ＴＢＳＡ）Ⅲ度烧伤致放射损伤（ＲＩ）、烧伤（ＢＩ）和放烧复合伤（ＲＢＩ）。观测伤后１、３、８、１６和２４ｈ大鼠心肌线粒体脂质过氧化和抗氧化酶活力的改变，发现三种伤类均表现为脂质过氧化代谢产物丙二醛（ＭＤＡ）含量伤后１ｈ即明显升高，２４ｈ降至对照组（Ｃ）水平；超氧化物歧化酶（ＳＯＤ）活力在整个实验过程中维持在明显低于Ｃ组的水平；ＢＩ组和     

TEP-87制剂对放烧复合伤小鼠造血功能的影响

健康小鼠受(60)~Co-γ线4Gy照射复合体表面积15％Ⅲ度烧伤。采用含生理需要量元素锌、铜、铁、赖氨酸、维生素B_1、B_2、C、D、叶酸及谷芽组合的TEP-87制剂,与主饲料(谷粉)按1∶9的比例配合,从伤前6天喂饲到伤后8天,通过观察骨髓粒系造血干细胞、外周血白细胞、骨髓有核细胞总数及T淋巴细胞非特异性酯酶染色阳性率,研究TEP-87制剂对造血及免疫功能的影响,结果证明;无论是单照伤或放烧复合伤,伤后1天TEP-87制剂对粒系造血干细胞有明显保护作用,伤后8天也表现出促进恢复作用。伤后1天白细胞总数也都保持在较高水平。但对骨髓有核细胞总数及T淋巴细胞非特异性酯酶染色阳性率,均未显示良好作用。TEP-87制剂对放烧复合伤小鼠造血的影响,可能是微量元素锌、铜、铁对造血干细胞增殖的刺激作用。     

WR-2721及胎肝对放烧复合伤小鼠的防治作用

重度放射损伤复合重度烧伤小鼠伤前给WR-2721[S-2(3-氨丙基氨基)乙基硫代磷酸]预防,伤后输注1×10~6同种异基因胎肝细胞悬液治疗,能明显降低动物死亡率,延长死亡动物平均活存日。与对照动物相比,外周血白细胞计数、骨髓有核细胞总数、骨髓CFU-C(粒系造血干细胞)损伤轻、恢复快。外周血酯酶染色的T淋巴细胞计数改变不如骨髓CFU-C变化明显。输注胎肝治疗合并应用辐射防护剂WR-2721对重度放射损伤复合重度烧伤的效果,超过单用胎肝细胞输注或WR-2721的效果。     

Effects of Combined Radiation-Burn Injury on Intestinal Epithelium in Mice

Seven hundred and forty-six mice,weighing 30-35g,were randomlydivided into 4 groups.The animals of the first three groups were inflicted withradiation injury(12 Gy total body irradiation from a ~(60)Co source),burn injury(15% TBSA third degree burns),and combined injury of the 2 agents ,mentionedabove respectively.The 4th group served as normal control.The mortality and the dynamic changes of the intestinal epithelium of theexperimental animals were studied.It was found that the clinical course of thecombined injury group was quite different from that of the other two.Themortality of the combined injury group was higher and the damage on theintestinal epithelium more severe than those of the radiation injury group in thefirst 48 h after infury.In the second 48 h,the mortality of the combined injurygroup became lower and recovery of the injured intestinal epithelium faster.