文书档案管理人员应加强修养、提高素质

正档案是人类活动的真实历史记录,是党和国家的宝贵财富,是进行两个文明和社会主义现代化建设的必要条件。加强文书档案管理人员思想、作风、业务的修养,对提高档案保管质量和利用价值,保证机关工作的高效,强化国家机器的正常运转,维护党和国家历史真实面貌,实现档案管理的制度化、规范化和标准化,推进档案管理的科学化和现代化具有重要的现实意义。     

文书档案管理人员应加强修养、提高素质

档案是人类活动的真实历史记录，是党和国家的宝贵财富，是进行两个文明和社会主义现代化建设的必要条件。加强文书档案管理人员思想、作风、业务的修养，对提高档案保管质量和利用价值，保证机关工作的高效，强化国家机器的正常运转，维护党和国家历史真实面貌，实现档案管理的制度化、规范化和标准化，推进档案管理的科学化和现代化具有重要的现实意义。     

重视医院基建档案管理

医院基建档案管理是医院档案管理中的一个重要组成部分,是反映医院基本建设历史和现状的真实记录。基建档案管理的好坏直接关系到医院整体建设和维修改造的速度、成本,甚至影响到建筑安全。及时、准确、完整、系统地做好基建档案的收集归档,科学地立卷,合理地保管,迅速地为利用者提供服务,是档案管理人员的职责。档案管理人员要尽职尽责,努力工作,使得基建档案管理更加科学化、规范化、标准化,为医院今后基建设施的安全使用及维修等方面能发挥重要作用。     

护理档案数字化管理的实践与思考

护理档案是护理工作的真实记录,是医院档案的重要组成部分.护理档案数字化管理从根本上改变了传统的护理档案管理模式,针对在护理档案数字化管理的实践现状和存在的问题,简析其原因,提出相应的对策,从而推动医院管理现代化水平,提高护理管理效能.     

关于基层医院档案材料鉴定的探讨

档案是人类社会实践活动和思维活动的真实记录,档案鉴定工作是档案归档工作的重要环节,通过分析档案鉴定现状、开展档案鉴定的益处及做好档案鉴定工作的对策等方面的探讨,提出了加强基层单位的档案鉴定工作对于推动档案管理工作的发展具有重要的意义.     

谈电子文件的归档在我院档案管理中的影响及应用研究

目的:主要探讨电子文件的归档在我院档案管理中的影响及应用。方法:首先从当前我院电子文件归档方式及途径的选择入手,加强对档案业务管理的影响,对档案学理论的影响及应采取的对策,包括做好归档电子文件的技术处理工作,实施电子文件管理战略,解决好电子文件的保存问题,档案管理向立体化方向发展,档案人员知识结构的变革等措施。结果:电子文件的归档得到了系统管理,实现了档案信息自动化及档案现代化的管理,解决了医院档案管理的薄弱环节;加快了医院档案管理工作的制度化和规范化进程,使医院档案管理工作取得重大提高。结论:加快电子文件的归档进程,实现档案工作各个环节的有机管理,更好地做好医院档案管理工作。     

浅谈医院档案管理的现状及档案信息自动化的管理对策

探讨本院档案管理的现状及存在的问题,找出解决问题的方法与档案信息自动化的对策。从当前本院档案管理的现状入手,加强档案管理工作,提高依法归档的自觉性,加强集中统一管理,建立科学的档案管理体系,健全档案管理制度,加强培训,加大投入,实现档案的现代化管理,建立档案三级网络管理模式。加强档案信息自动化,提高系统的整体水平,有重点地抓好数据库建设。通过以上措施本院档案得到了系统管理,实现了档案信息自动化及档案现代化的管理,解决了档案管理的薄弱环节;加快了档案管理工作的制度化和规范化进程,使本院档案管理工作取得重大提高。加快档案信息自动化系统建设,实现档案工作各个环节的计算机化,使本院档案工作实现现代化,可更好地做好新时期档案管理工作。     

以现代化信息手段加强医院档案管理

随着现代化医院信息化建设的不断发展，置身于医院信息集成系统中，医院档案工作者应该认识到医院档案和档案工作发生了深刻的变化。本文试从我院信息化建设的实际情况，谈以现代化手段加强医院档案管理工作。[第一段]     

我院档案管理工作的做法与体会

胡锦涛同志曾在全国档案工作暨表彰先进会议上的讲话中指出:“档案是人类活动的真实记录,是人们认识和把握客观规律的主要依据。借助档案,我们能够更好地了解过去,把握现在,预见未来。档案事业是党和国家事业发展的一个不可缺少的方面。”医院档案工作是医院一项重要的基础性工作,它不但反映医院的发展、建设情况,而且对于保证医院工作的连续性和反映医院的历史面貌都具有十分重要的意义。它在医院管理中扮演着重要的角色,它取之于医院又用之于医院,并伴随医院发展而发挥着越来越重要的作用。1我院档案工作实行规范化管理迫在眉睫我院是广西最大的集医疗、教学、科研及预防保健于一体的综合性医院,拥有广西一流的技术、一流的人才、一流的设备。但前几年,由于种种原因,我院档案管理工作一直比较滞后,表现在:办公设备不齐全或不完善、库房要求不合格、人才匮乏、管理制度不健全、档案资料未按时归档等等。这与我院的医疗、教学、科研工作相比极不相称,与医科大学第一附属医院在广西的地位也格格不入。档案管理工作的滞后,也给医院带来不少损失。如:我院旧干部病房建成于1986年,当时没有安装空调,随着社会发展,人们生活水平的提高,2002年为照顾老同志,让他们能在冬暖夏凉的...     

加强医院档案现代化管理

医院档案管理是医院管理的基础性工作。面对日益深化的医疗改革,实现医院档案管理现代化,迎接新的挑战,更好地服务于医疗、科研、教学工作,是目前医院档案工作的新任务、新课题,是新形势下对医院提出的迫切要求。1医院档案管理的意义1.1档案是我们在工作实践中认识和把握客观规律的重要依据。借助档案,我们能够更好地了解过去,把握现在,预见未来。因此,档案管理是我们医院的一项重要的不可或缺的工作。1.2档案是医疗工作的真实记录。医院病案不但是医院的重要资料而且是国家的重要档案,它集中反映了医院对病人的治疗过程,反映了我国医药卫生…     

UniPron is A Fully Effective Non-hormonal Reversible Contraceptive in Baboon Model （Papio Anubis）

Objective To determine the safety and efficacy of UniPron as a reversible contraceptive.
Methods Vaginal swabs were obtained before and after UniPron administration, cultured onto appropriate culture media and bacteria identification was done based on type of media used, Gram stain reactions, colony morphology and biochemical tests. Vaginal biopsy tissues were processed using paraffin wax method, stained with hematoxylin and eosin and examined under light microscopy to determine the effect of the product on vaginal tissues. The effect of UniPron on sperm was examined by mixing the product with electroejaculated spermatozoa in vitro at different concentrations. For efficacy studies, male baboons of proven fertility were mated with UniPron treated or untreated females of proven fertility during the fertile stages.
Results All the five females （100%） that were treated with UniPron did not conceive and they regained total fertility when the treatment was stopped while all the controls conceived. At a concentration of 40%, UniPron completely immobilized spermatozoa in an in-vitro system. UniPron mechanism of action was by lowering the vaginal pH and on application in baboon, the pH was lowered for at least 3 h after which it went back to normal.
Conclusions As we plan for a study to test UniPron as a microbicide to prevent STIs including HIV, our current study has established that this novel product is effective in contraception and harmless to vaginal tissues and vaginal microbial flora in a baboon model （Papio anubis）.     

Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata （L,） Stapf in rats

OBJECTIVE： In continuation to the growing evidence for therapeutical potential of Desmostachya bipinnata （Linn） Stapf, the current pharmacological study was carried out to evaluate the diuretic and laxative activity of its hydro-alcoholic extract in rats. METHODS： The hydro-alcoholic extract of D. bipinnata whole plant was prepared by using Sox- hlet extractor and subjected to analysis by standard preliminary phytochemical tests. Evaluation of both diuretic and laxative activity was carried out using standard methods as reported earlier. Frusemide （20 mg/kg） was served as positive control for diuretic activity and sennosides （10 mg/kg） served as negative control for laxative activity. RESULTS： The hydro-alcoholic extract showed significant diuretic activity and was found to be the most potent in increasing the urinary output at 500 mg/kg when the effect was compared with that of the standard frusemide （P〈0.01）. Moreover, this extract was found to be most effective in increasing urinary electrolyte concentration （Na＋, K＋, and Cl-） at both doses tested. Whereas the results for laxative activity showed minimal increase of feces output at the dose of 500 mg/kg and the increase was negligible when compared with that of the standard drug sennosides. CONCLUSION： Altogether, the above significant findings validate and support its folkloric diuretic use and lend pharmacological credence to the ethno-medical use of this plant in traditional system of medicine, which demands further studies to investigate its active constituents, as well as its use and safety.     

Effect of the disruption of three cytoskeleton components on chondrocyte metabolism in rabbit knee cartilage

Background Chondrocytes＇ phenotype and biosynthesis of matrix are dependent on having an intact cytoskeletal structure.Microfilaments,microtubules,and intermediate filaments are three important components of the cytoskeletal structure of chondrocytes.The aims of this study were to determine and compare the effects of the disruption of these three cytoskeletal elements on the apoptosis and matrix synthesis by rabbit knee chondrocytes in vitro.Methods Chondrocytes were isolated from full-thickness knee cartilage of two-month-old rabbits using enzymatic methods （n=24）.The isolated cells were stabilized for three days and then exposed to low,medium,and high doses of chemical agents that disrupt the three principal cytoskeletal elements of interest：colchicine for microtubules,acrylamide for intermediate filaments,and cytochalasin D for actin microfilaments.A group of control cells were treated with carrier.Early apoptosis was assessed using the Annexin-FITC binding assay by flow cytometry on days 1 and 2 after exposure to the disrupting chemical agents.The components and distribution of the cytoskeleton within the cells were analyzed by laser scanning confocal microscopy （LSCM） with immunofluorescence staining on day 3.The mRNA levels of aggrecan （AGG） and type Ⅱ collagen （Col-2） and their levels in culture medium were analyzed using real-time PCR and enzymelinked immunosorbent serologic assay （ELISA） on days 3,6,and 9.Results In the initial drug-dose-response study,there was no significant difference in the vitality of cells treated with 0.1 μmol/L colchicine,2.5 mmol/L acrylamide,and 10 μg/L cytochalasin D for two days when compared with the control group of cells.The concentrations of colchicine and acrylamide treatment selected above significantly decreased the number of viable cells over the nine-day culture and disrupted significantly more cell nuclei.Real-time PCR and ELISA results showed that the mRNA levels and medium concentrations of AGG and Col-2 were significantly decreased for     

Liuwei Dihuang,a traditional Chinese herbal formula,suppresses chronic inflammation and oxidative stress in obese rats

OBJECTIVE： To investigate the anti-inflammatory, anti-oxidative stress, and adipokine-ameliorating effects of Liuwei Dihuang （LWDH）, a traditional Chinese herbal formula, in obese rats. METHODS： After 2 weeks of acclimation with free access to regular rodent chow and water, obese-prone-caesarean-derived （OP-CD） rats were fed a modified AIN-93G diet containing 60% energy from fat. Treatment was performed twice daily by gavage feeding with 500, 1 500, or 3 500 mg/kg body weight LWDH suspended in water （n=12 rats per group）. Twelve obese-resistant-CD （OR-CD） rats were fed the atherogenic diet and gavaged with water, and served as the normal control. Blood biomarkers of inflammation, oxidative stress and adiponectin were measured post-sacrifice and used to determine the treatment effect of LWDH and assess the suitability of OR/OP-CD rats for studying these parameters. RESULTS： After 9 weeks of treatment, LWDH lowered serum C-reactive protein （CRP） and tumour necrosis factor-α （TNF-α） levels. Serum interleukin-6 （IL-6） levels showed a tendency towards reduction, but were not significantly different from the OP-CD control. Liver superoxide dismutase （SOD） activity was increased in response to all three doses of LWDH, while the levels of reduced （GSH） and oxidized glutathione （GSSG） and thiobarbituric acid reactive substances （TBARS） were unchanged. Serum adiponectin levels were increased in response to oral administration of LWDH at the dose of either 500 or 1 500 mg/kg body weight. In addition, comparisons between OR-CD and OP-CD rats revealed differential, and for some biomarkers, conflicting characteristics of high-fat diet-fed OP-CD rats in reference to obese human subjects in terms of inflammatory and oxidative stress biomarkers and circulating adiponectin levels. CONCLUSION： The results show, for the first time, the anti-inflammatory, anti-oxidative stress and adiponectin-ameliorating effects of LWDH in obese rats. The suitability of the OP-JOP-CD rat model as     

New Mechanism of Herb-herb Interaction between Ginseng and Trogopterus Based on CYPs in Rat Livers

The study was designed to investigate the potential mechanism of herb-herb interaction between ginseng and Trogopterus （Trg） based on Cytochrome P450 isozymes （CYPs） in rat livers. We estimated the influence on CYP1A2, CYP2E1, and CYP3A1/2 activity caused by ginseng and Trg used in combination. The CYP1A2 and CYP2E1 enzyme activity were induced by ginseng and Trg used in combination. And this induction effect was caused via inducing CYP1A2 and CYP2E1 protein expression which was supposed caused by inducing the gene expression of CYP1A2 and CYP2E1.     

Iloprost inhibits fracture repair in rats

Background Previous studies have shown that prostaglandins （PGs） dramatically stimulate healing processes in bone.However,the effect of prostaglandin l2 （PGI2） on fracture healing remains unclear.To investigate the effect of PGI2,a study on fracture healing process in closed tibia fractures was designed.Methods Thirty-six Sprague-Dawley male rats were randomized into two groups.On the first day,their right tibias were fractured by three-point bending technique.The study group （n=18） received a single injection of 10 μg/kg iloprost for 5 days,while the control group （n=18） received saline solution in the same way.On the 7th,14th and 28th days following the fracture,six rats were sacrificed and their right legs were harvested in each group.The progression of fracture healing was assessed for each specimen by the scores of radiography （by Lane-Sandhu） and histology （by Huo et al）.Results On the 7th day,the radiographic and histologic scores were equal.On the 14th day radiographic total score was 6 and histologic total score was 23 in the iloprost group,whereas radiographic total score was 11 and histologic total score was 33 in the control group.On the 14th day radiographic and histologic scores were significantly decreased in the iloprost group compared to the control group （P 〈0.05）.On the 28th day radiographic total score was 12 and histologic total score was 37 in the iloprost group,whereas radiographic total score was 15 and histologic total score was 40 in the control group.On the 28th day although there was a decrease in radiographic and histologic scores of the iloprost group acording to control group,it was not statistically significant （P 〉0.05）.Conclusion Iloprost delays fracture healing in early stage in rats.     

Effect of Shenfu on the Expression of Bcl-2 and Bax of Intestinal Mucosal Epithelial Cells after Ischemic Reperfusion in Rats

Objective To study the effect of Shenfu on the expression of bax and bcl-2 in small intestinal mucosal epithelial cells after ischemic reperfusion injury and explore the effect of Shenfu on small intestinal mucosal epithelial cells apoptosis. Methods 36 SD rats were randomized into three groups, each consisting of 12 rats： Sham group （S group）, Ischemic reperfusion group （IR group）, Shenfu group （SF group）, Ischemic reperfusion models were made by ligated the superior menseneric artery for 1 hour followed by 2 hrs reperfusion. Histological mucosal damage in each group was graded according to Chiu＇s score. Immunohistochemistry detected the expression of bax and bcl-2, and obtained the optical density （OD） value using a color image pattern analysis system, and then calculated the ratio of bcl-2/bax. TUNEL method measured apoptotic intestinal mucosal epithelial cells, and calculated the apoptotic index. Results There were edema and epithelial impairment in part of villus and the integral was higher in SF group than S group （P〈0.05）, but lower than IR group （P〈0.01）. Compared IR group with S group and SF group, the average OD value of bcl-2 and bax had significant statistic difference （P〈0.01）, and the average OD value of bcl-2 in SF group was higher than in S group （P〈0.05）, meanwhile bcl-2/bax was significantly lower in IR group than in S group and SF group （P〈0.01）, but that was higher in SF group than in S group （P〈0.05）. Apoptotic index in IR group was significantly higher than that in other two groups （P〈0.01）, and that in SF group was higher than in S group （P〈0.05）. Conclusion Shenfu can enhance the expression of bcl-2, decrease the expression of bax, meanwhile increase the ratio of bcl-2/bax, inhibit small intestinal mucosal epithetlial cells apoptosis, and protect small intestine mucosal epithetlial after ischemic reperfusion.     

补肾活血方对PCOS大鼠模型卵巢中PAI-1mRNA表达的影响

目的探讨补肾活血方对大鼠PCOS模型卵巢局部纤溶酶原激活物抑制因子-1（PAI—1mRNA）表达的影响。方法选用未成年24d龄SD雌性大鼠60只,随机分为模型组、克罗米酚组、补肾活血方高剂量组、补肾活血方低剂量组、正常对照组5组。用Bogovich法建立大鼠多囊卵巢病理模型。以克罗米酚为对照。用原位杂交法观察补肾活血方对多囊卵巢大鼠局部PAI—1mRNA的影响。结果模型组卵巢局部PAI—1mRNA存在卵泡膜间质细胞显著增高,用补肾活血方高、低剂量与克罗米酚药后,卵巢局部PAI-1mRNA的表达明显降低．差异具有统计学意义（P〈0．05、P〈0．01）。补肾活血方高剂量组与克罗米酚组比较,差异具有显著统计学意义（P〈0．01）;低荆量组与克罗米酚组比较差异无统计学意义（P〉0．05）,补肾活血方高、低剂量组比较,低剂量组卵泡膜间质细胞上PAI-1的基因表达增高更明显,但二者差异无统计学意义（P〉0．05）。结论PAI-1mRNA可能与多囊卵巢综合征的发病机制有关。以补肾活血立法的补肾活血方能降低多囊卵巢大鼠局部PAI—1mRNA的显著增高表达．降低PAI—1mRNA卵巢局部的作用。提示补肾活血方可能通过PAI—1mRNA途径促进卵巢排卵的机制。     

表皮生长因子受体酪氨酸激酶抑制剂在非小细胞肺癌应用的瓶颈——获得性耐药

以表皮生长因子受体酪氨酸激酶抑制剂（epidermal growth factor receptor-tyrosine kinase inhibitor,EGFR-TKI）分子靶向治疗研究为肇始,转化性研究使基础实验和临床实践间的鸿沟迅速填平,改变着人们认识治疗肺癌的视角。无可否认,EGFR-TKI上市后极大地延伸了肿瘤学家治疗肺癌的手段,但不管从临床经验、临床研究数据、分子生物学层面还是文献计量学的角度,     

益智健脑颗粒联合针灸对阿尔茨海默病大鼠学习记忆的影响

目的观察益智健脑颗粒联合针灸对阿尔茨海默病（Alzheimers disease,AD）大鼠学习记忆的影响。方法将大鼠随机分为假手术组（A组）、模型组（B组）、针灸组（C组）、益智＋针灸组（D组）各10只,B、C、D 3组分别以海马CA1区注射β淀粉样蛋白25-35（Aβ25-35）造模,A组注射等量的双蒸水,各组分别治疗20 d后行Morris水迷宫试验,观察大鼠学习记忆能力变化。结果B组较A组的平均潜伏期明显延长,差异具有统计学意义（P〈0.05）;与B组比较,C组、D组的平均潜伏期明显缩短,过台次数增多,差异具有统计学意义（P〈0.05,P〈0.01）;与C组比较,D组的潜伏期缩短,过台次数增多,差异具有统计学意义（P〈0.05）。结论益智健脑颗粒联合针灸能够提高Aβ25-35介导的AD模型大鼠的学习记忆能力。