非小细胞肺癌患者放疗前后机体免疫功能变化的研究

目的：报道３０例非小细胞肺癌（ＮＳＣＬＣ）患者放疗前后免疫功能的变化。方法：采用双抗体夹心法和比浊法检测血清中ｓＩＬ－２Ｒ、免疫球蛋白（ＩｇＧ,ＩｇＡ,ＩｇＭ）含量,用单克隆抗体和流式细胞仪技术检测外周血中Ｔ细胞亚群,Ｂ细胞,ＮＫ细胞百分数;与健康人做对照。结果：放疗前ｓＩＬ－２Ｒ显著增高（Ｐ〈０．０１）,ＩｇＧ及ＩｇＭ６亦增高（Ｐ〈０．０５）,Ｂ细胞百分数低于（Ｐ〈０．０１）,ＣＤ３＾＋细胞降低     

分泌抗rHuIL—6McAb杂交瘤细胞系的建立及其应用研究

应用常规方法建立了４株稳定分泌抗重组人ＩＬ－６（ｒＨｕＩＬ－６）单克隆抗体（ＭｃＡｂ）小鼠杂交瘤细胞系１Ｈ３、２Ａ１０、３Ａ３和４Ｂ１。其中，１Ｈ３为ＩｇＧ２ｂ（Ｋ），２Ａ１０为ＩｇＧ１（Ｋ），３Ａ３和４Ｂ１为ＩｇＧ２ａ（Ｋ）。４株ＭｃＡｂ特异性强，与细胞因子ＩＬ－１β、ＩＬ－３、ＩＬ－８、ＴＮＦ－α、ＧＭ－ＳＣＦ、ＩＣＡＭ－１，以及受体菌菌体蛋白成分均无交叉反应。间接ＥＬＩＳＡ测定小鼠腹水ＭｃＡ     

肿瘤碱性蛋白单克隆抗体免疫学特性的研究及其应用

应用淋巴细胞杂交瘤技术，建立了４株抗人血清肿瘤碱性蛋白（Ｔｕｍｏｕｒｂａｓｉｃｐｒｏ－ｔｅｉｎ，ＴＢＰ）杂交瘤细胞株（１Ｃ３、４Ｆ４、５Ｆ４、３Ｇ９），并以制备的单克隆抗体（ＭｃＡｂ）对其抗原决定簇及免疫学特性进行了分析。Ｉｇ亚类测定：均为ＩｇＧ２ａ，腹水效价为１×１０－６～１×１０－８。特异性测定：ＴＢＰＭｃＡｂ与ＩｇＧ、ＩｇＡ、ＩｇＭ和Ａｌｂ无交叉反应。单抗相加试验证实：５Ｆ４、４Ｆ４和１Ｃ３为识别ＴＢＰ上同一抗原决定簇，３Ｇ９则为识别ＴＢＰ上另一抗原决定簇。分别利用单株和混合株ＭｃＡｂ标酶建立了可应用于人血清ＴＢＰ含量测定的ＥＬＩＳＡ双抗体夹心法，并用于人血清ＴＢＰ含量测定。     

rhIL—2调节离体boPBMC增殖和分泌Ig的作用

应用＾３Ｈ－ＴｄＲ掺入法，ＥＬＩＳＡ和ＦＡＣＳ研究了ｒＨＩＬ－２调节离体奶牛外周血单核细胞（ｂｏＰＢＭＣ）免疫应答的特点。ｒｈＩＬ－２能诱导细胞持续性增殖和分泌Ｉｇ，尤其是分泌ＩｇＡ和ＩｇＧ２（与ＰＷＭ相比，Ｐ〈０．０１）；还能增强ＰＷＭ诱导的Ｉｇ的分泌，但不改变ＳＥＢ抑制Ｉｇ分泌的作用。细胞表型也表明，ｒｈＩｌ－２对ＰＷＭ或ＳＥＢ诱导的ｂｏＰＢＭＣｋ中ＣＤ４或ＣＤ８细胞增殖作用无选择性。     

分泌抗rHuIL－6McAb杂交瘤细胞系的建立及其应用研究

应用常规方法建立了４株稳定分泌抗重组人ＩＬ－６（ｒＨｕＩＬ－６）单克隆抗体（ＭｃＡｂ）的小鼠杂交瘤细胞系１Ｈ３、２Ａ１０、３Ａ３和４Ｂ１。其中，１Ｈ３为ＩｇＧ２ｂ（ｋ），２Ａ１０为ＩｇＧ１（ｋ），３Ａ３和４Ｂ１为ＩｇＧ２ａ（ｋ）。４株ＭｃＡｂ特异性强，与细胞因子ＩＬ－１β、ＩＬ－３、ＩＬ－８、ＴＮＦ－α、ＧＭ－ＳＣＦ、ＩＣＡＭ－１，以及受体菌菌体蛋白成分均无交叉反应。间接ＥＬＩＳＡ测定小鼠腹水ＭｃＡｂ效价为１０（－６）～１０（－８）。应用识别不同表位的ＭｃＡｂ建立了双抗体夹心ＥＬＩＳＡ法检测ＩＬ－６，敏感性为１００ｐｇ／ｍｌ。初步应用表明可用于临床标本的检测。     

rhIL－2调节离体boPBMC增殖和分泌Ig的作用

应用￣３Ｈ－ＴｄＲ掺入法、ＥＬＩＳＡ和ＦＡＣＳ研究了ｒＨＩＬ－２调节离体奶牛外周血单核细胞（ｂｏＰＢＭＣ）免疫应答的特点。ｒｈＩＬ－２能诱导细胞持续性增殖和分泌Ｉｇ，尤其是分泌ＩｇＡ和ＩｇＧ＿２（与ＰＷＭ相比，Ｐ＜０．０１）；还能增强ＰＷＭ诱导的Ｉｇ分泌，但不改变ＳＥＢ抑制Ｉｇ分泌的作用。细胞表型也表明，ｒｈＩＬ－２对ＰＷＭ或ＳＥＢ诱导的ｂｏＰＢＭＣ中ＣＤ４￣＋或ＣＤ８￣＋细胞增殖作用无选择性。     

重组人红细胞生成素单克隆抗体的制备

本文应用常规淋巴细胞杂交瘤技术制备了４株能稳定分泌抗人重组红细胞生成素（ｒＨｕＥＰＯ）单克隆抗体（ＭｃＡｂ）的小鼠杂交瘤细胞系ＢⅡ１Ｂ５、ＤⅡ６Ｂ９、ＭⅡ１Ｈ４和ＧＩ３Ｅ７。用鼠单克隆抗体分型试剂盒鉴定，其分泌的ＭｃＡｂ的类分别是ＩｇＭ、ＩｇＭ、ＩｇＧ１和ＩｇＧ２ａ。间接ＥＬＩＳＡ法测定细胞上清的效价为１×１０－２～１．２５×１０－４，腹水效价为１×１０－２～１×１０－８。培养上清经ＥＬＩＳＡ鉴定，与ＩＬ－２、ＧＭ－ＣＳＦ、ＩＦＮ－α等细胞因子均无交叉反应，只与ｒＨｕＥＰＯ特异性结合。     

大肠癌患者围手术期免疫状态变化的临床意义

目的 研究大肠癌患者围手术期的免疫状态。方法 应用ＡＰＡＡＰ夹心法ＥＬＩＳＡ和比色法分别测定大肠癌患者手术前后Ｔ细胞亚群变化,血浆可溶性白介２受体（ｓＩＬ－２Ｒ）及一氧化氮（ＮＯ）和癌组织的ＳＩＬ－２Ｒ,ＮＯ含量。结果 大谫ＣＤ３,ＣＤ４细胞、ＣＤ４／ＣＤ８比值和血浆ＮＯ水平明显低于正常,血浆ＳＩＬ－２Ｒ、ＣＤ８细胞较正常显著增高,肿瘤切除后３周左右ＣＤ３、ＣＤ４细胞、ＣＤ４／ＣＤ８和血浆术前有明     

巨细胞病毒感染与可溶性白细胞介素2受体的关系

应用酶联免疫吸附试验（ＥＬＩＳＡ）对１０４例育龄妇女的血清进行了巨细胞病毒（ＨＣＭＶ）ＩｇＧ、ＩｇＭ抗体的检测，同时用ＥＬＩＳＡ双抗体夹心法测定了不同感染状态下血清中可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）的水平，并将ｓＩＬ－２Ｒ水平与未感染ＨＣＭＶ的正常育龄妇女进行了比较。结果，育龄妇女中抗－ＨＣＭＶＩｇＧ的阳性率为８９．４％，ＩｇＭ的阳性率为９．６％，感染ＨＣＭＶ的妇女血清中ｓＩＬ－２Ｒ水平均大于未感染的对照组（１７８．１±５７．３Ｕ／ｍｌ），Ｐ＜０．０５，其中ＩｇＭ阳性者和ＩｇＭ、ＩｇＧ同时阳性者血清中ｓＩＬ－２Ｒ水平最高，分别为９１０±４６５．６Ｕ／ｍｌ和９０５±３４７．８Ｕ／ｍｌ，两者间的差异无显著性意义（Ｐ＞０．０５），但均大于仅抗－ＨＣＭＶＩｇＧ阳性者（４４６．８±１５８．９Ｕ／ｍｌ），Ｐ均＜０．０５。表明，ＨＣＭＶ感染可致ｓＩＬ－２Ｒ水平升高，并且活动性感染者上升明显。提示：ｓＩＬ－２Ｒ可能参与了ＨＣＭＶ的免疫致病机制。     

Epstein—Barr病毒潜伏膜蛋白2A重组痘苗病毒转染DC及特异性CTL的体外诱导

通过ＥＢ病毒ＬＭＰ２Ａ重组痘苗病毒转染的ＤＣＳ体外诱导ＬＭＰ２Ａ特异性ＣＴＬ,并通过ＧＭ－ＣＳＦ、ＩＬ－４和ＴＮＦ－ａ培养体系,我们诱导出了人外周血单核细胞来源的ＤＣ。同时选用在鼻咽癌患者中表达的ＥＢ病毒潜伏蛋白之一ＬＭＰ２Ａ作为靶基因,利用重组痘苗病毒转染诱导的ＤＣＳ。ＤＣＳ与自体ＰＢＭＣＳ混合培养,在ＩＬ－２的刺激作用下获得特异性ＣＴＬ。结果如下：１．人外周血单核细胞经ＧＭ－ＣＳＦ、ＩＬ－４、ＴＮＦ－ａ的混合培养,１０天可获得成熟的功能性ＤＣＳ。ＦＡＣＳ检测显示ＤＣ表面相对特异性标志ＣＤ８３…     

DEN-2 NGC株感染BALB/c小鼠TH1/TH2类细胞因子产生的差异

目的通过观察2型登革病毒(DEN2)NGC株初次感染和再次感染BALB/c小鼠后小鼠血浆中TH1和TH2类细胞因子的产生及其动态变化,研究DEN2感染的免疫机制。方法用不同量的DEN2NGC株经皮下多点注射,建立BALB/c小鼠感染模型,间接ELISA法测定感染后不同时间小鼠血浆IL-2、IFN-γ、IL-4、IL-5、IL-6、IL-10的含量。结果(1)在初、再次感染阶段,DEN2NGC株各感染组产生IL-4、IFN-γ、IL-2、IL-5、IL-6、IL-10与正常对照组有显著差异(P<0·05)。①在初次感染阶段,NGC株各感染组血浆IL-2、IL-5、IL-6于第4、6、8天出现;在再次感染阶段,各感染组产生IL-2、IL-5、IL-6均于第1天迅速升高;IL-2水平于第4天达高峰(104448·46±13314·1pg/ml),维持5~7天后迅速下降;IL-5于48小时达到高峰(135·125±46·75pg/ml);各组小鼠IL-6产生的动态相似,在再次感染后第1天,达高峰(555·823±44·639pg/ml)后逐渐下降。②初次感染早期,IL-4水平明显升高,而IFN-γ处于较低水平;再次感染后第1天,IL-4水平达最高峰(4294·668±349·038pg/ml),IFN-γ则于第4天和第11天达较高水平,两者的产生彼消此长,且产生动态与感染病毒量有关。(2)在初、再次感染阶段,用不同量DEN2NGC株感染组产生细胞因子水平有差异。结论DEN2NGC株初次和再次感染BALB/c小鼠后,TH1和TH2应答均可发挥一定作用,二者相互影响,TH细胞及其产生的CK在DEN感染的发病机制中起重要作用。     

Differential effects of interleukin-12 on the development of naive mouse CD4+ T cells

The influence of interleukin (IL)-12 and IL-4 on the differentiation of naive CD4⁺ T cells was studied in an accessory cell-free in vitro system. Dense CD4⁺ T cells were purified from unimmunized mice and activated using immobilized anti-CD3 monoclonal antibodies (mAb) in the presence of IL-4, IL-12, or a combination of both cytokines, and restimulated after 6 days by re-exposure to anti-CD3-coated culture wells. T cells initially activated in the presence of IL-4 produced substantial amounts of IL-4 and trace amounts of interferon (IFN)-γ after restimulation at day 6 with plate-bound anti-CD3 mAb. By contrast, T cells primed in the presence of IL-12 produced high levels of IFN-γ and only minimal amounts of IL-4, thus indicating that IL-12 and IL-4 by acting directly on stimulated naive CD4⁺ T cells support the development of T_H1 and T_H2 cells, respectively. When naive CD4⁺ T cells were stimulated in the presence of IL-12 together with IL-4 in comparable concentrations, the effect of IL-12 on T_H1 differentiation was largely inhibited by IL-4. On the other hand, IL-12 exerted no inhibitory effect on IL-4-induced T_H2 differentiation but rather enhanced the production of IL-4 after restimulation of the respective T cells. Decreasing amounts of IL-4 in combination with a high level of IL-12 led to an increasing production of IFN-γ by the emerging T cells and, simultaneously, to a relatively high production of IL-4. These data were confirmed by time-course experiments which revealed that the delayed addition of IL-4 to IL-12-primed T cell cultures resulted in a gradual restoration of IFN-γ production whereas in parallel the secretion of IL-4 was not reduced over a wide period of delay (6–72 h). These results, therefore, demonstrate that (a) IL-4 dominates the effect of IL-12, (b) IL-12 promotes the development of T_H1 cells; however, in the presence of IL-12 and relatively high levels of IL-4 also the development of T_H2-like cells is slightly but significantly enhanced by IL-12, and (c) high amounts of IL-12 in combination with relatively low levels of IL-4 give rise to a T cell population that upon rechallenge exhibited a cytokine profile resembling that of T_H0 cells.     

微囊化转mIFN-γ基因CHO细胞皮下移植促进荷瘤小鼠TH1/TH2的漂移

目的：通过观察微囊化转mIFN-γ基因CHO细胞皮下移植对荷瘤小鼠TH1／TH2类细胞因子的影响，进一步了解外源性IFN－γ对TH1／TH2平衡的调节作用，同时验证微囊化基因工程细胞移植治疗恶性肿瘤的可行性。方法：将mIFN-γ基因转染到正常细胞CHO，然后进行微囊化，移植到荷瘤小鼠的皮下，2周后，测定小鼠血清TH1和TH2类细胞因子IFN－γ、IL－2、IL－12、IL－4、IL－10的水平，并与对照组相比较。同时测量肿瘤的平均直径，以观察肿瘤的生长速度，并观察荷瘤小鼠的生存期。结果：经微囊化转mIFN-γ基因CHO细胞皮下移植干预治疗后，小鼠血清TH1类细胞因子IFN－γ、IL－2、IL－12水平明显升高，而TH2类细胞因子IL－4、IL－10变化不明显；小鼠肿瘤的生长速度明显减慢，生存期明显延长。结论：外源性IFN－γ促进荷瘤小鼠TH1细胞的分化，使TH1／TH2向着TH1方向漂移；微囊化基因工程细胞的移植，有望替代基因工程药物，成为治疗恶性肿瘤的又一新平台。     

TH1/TH2细胞因子与习惯性流产的关系研究

目的通过测定习惯性流产患者（RSA）及正常妊娠妇女外周血血清TH1/TH2细胞因子的含量,探讨TH1/TH2细胞因子与习惯性流产发生的关系。方法采用酶联免疫吸附法检测30例RSA患者,72例正常妊娠妇女血清IL-2、IL-4、IL-10、IFN-γ、TNF-α及TNF-β水平并比较两组之间的差异。结果RSA患者IL-2、IL-4、IL-10、IFN-γ、TNF-α血清水平显著高于正常妊娠妇女（P〈0.05）,TNF-β血清水平显著低于正常妊娠妇女（P〈0.05）。结论血清IL-2、IL-4、IL-10、IFN-γ、TNF-α水平可能在习惯性流产发生中起重要作用。     

呼吸道合胞病毒下呼吸道感染TH亚群功能状态的研究

目的 探讨RSV下呼吸道感染TH亚群功能状态。方法 用单克隆抗体双色免疫荧光PE／FITC双标记，流式细胞仪检测30例RSV下呼吸道感染患儿急性期外周血单个核细胞（PBMCs)辅助性T淋巴细胞CD4^ 及其亚群CD4^ CD8RA^ 、CD4^ CD45RO^ 的表达，ELISA方法检测血清中IFN－γ、IL－4，IgE水平。结果 RSV下呼吸道感染组患儿外周血辅助性T淋巴细胞CD4^ 明显低于对照组（P＜0．05），CD4^ CD45RA^ 细胞明显下降（P＜0．05），CD4^ CD45RO^ 细胞虽有下降，但与对照组相比无统计学差异（P＞0．05）。CD4^ CD45RA^ /CD4^ CD45RO^ 比值与对照组相比明显降低（P＜0．05）。RSV下呼吸道感染组患儿血清中IFN－γ，IL－4水平均有降低，其中IFN－γ水平下降非常明显（P＜0．01），IFN－γ/IL-4比值降低（P＜0．05）。RSV下呼吸道感染组患儿血清中IgE水平明显升高。结论 婴幼儿RSV下呼吸道感染急性期的免疫功能紊乱主要表现为辅助性T淋巴细胞CD4^ 及其亚群CD4^ CD45RA^ /CD4^ CD45RO^ 的失衡。TH1及其功能下降，TH2及其功能相对增强，TH1／TH2失衡是导致该病发生TH2样反应重要的免疫机制。     

Tyrosine hydroxylase-immunoreactive neurons of the hypothalamus: A light and electron microscopic study

The localization and morphology of neurons, processes, and neuronal groups in the rat hypothalamus containing tyrosine hydroxylase-like immunoreactivity were studied using an antiserum to bovine tyrosine hydroxylase. This antiserum was thoroughly characterized by precipitation of enzyme activity, immunoblotting, and precipitation of cell-free translation products; a single molecular weight band was recognized by the antiserum. Absorption of the antiserum with purified tyrosine hydroxylase abolished immunocytochemical staining, while addition of bovine dopamine β-hydroxylase had no effect on immunostaining.Immunoreactive cells were found throughout the hypothalamus. Significant numbers of cells were found in the arcuate, periventricular, dorsomedial hypothalamus/zona incerta, posterior hypothalamic regions (A11–A14), and paraventricular nucleus, as previously described, and in addition, in the preoptic area, adjacent to the anterior commissure, medial and lateral to the suprachiasmatic nucleus, dorsal to and in the supraoptic nucleus, at the lateral borders of the ventromedial nucleus, and in the dorsal and ventral lateral hypothalamus. None of the immunoreactive cell groups are totally separated from adjacent cell groups. Dendritic overlap occurs between any two adjacent groups.From cell counts of 30 μm coronal sections, we estimate the hypothalamus has about 12,000 cells based on raw counts, or 8000 immunoreactive cells after correction for possible split cells. Mean soma size varied considerably from one immunoreactive group to another. Cells in the caudal part of the dorsomedial hypothalamus/zona incerta region were the largest, with a mean diameter of 25 μm, while cells in the anterior commissural and posterior hypothalamic group were among the smallest, with mean diameters of 10 μm. The largest immunoreactive cells in the hypothalamus had volumes in excess of ten times greater than the smallest immunoreactive cells.Tyrosine hydroxylase immunoreactivity was found in dendrites in every region of the hypothalamus, sometimes extending hundreds of micrometers from the perikaryon of origin. Although adjacent cell groups were not distinctly separated, the dendritic arbors of the different cell groups differed greatly. Dendritic and somatic appendages were found on some cells, particularly in the paraventricular nucleus. Immunoreactive dendritic arbors were particularly large in cells seen on horizontal sections through the caudal dorsomedial hypothalamic group and through the anterior hypothalamus. Only slight dendritic trees were observed in the rostral dorsomedial hypothalamus/zona incerta region, and in the pericommissural group.Immunoreactive axons, containing dopamine, norepinephrine or epinephrine, were found in every area of the hypothalamus. Axon morphology, size, density and terminals varied from region to region. Axons of hypothalamic origin arose from both immunoreactive somata and primary dendrites. A large number of non-synapsing immunoreactive axons were found in the median eminence, and many were also found throughout the hypothalamus. Retrograde transport of substances injected in the neurohypophysis labeled cells in the most rostral part of the arcuate nucleus and periventricular area; some labeled cells also showed tyrosine hydroxylase immunoreactivity. Many of the immunoreactive boutons contained primarily clear vesicles, while others contained both clear and large dense-core vesicles. Immunoreactive axons made synaptic contact with unlabeled dendrites.     

TH5对雄性大鼠性行为与血清性激素水平影响的研究

为了开发避孕新药,本文报告昆明山海棠提取物ＴＨ５对Ｗｉｓｔａｒ成年雄性大鼠抗生育试验性行为的影响,并测定血清性激素水平。灌服ＴＨ５（１１６ｍｇ／ｋｇ／ｋｇ／ｄ,共３０ｄ）的雄性大鼠与成年雌性大鼠合笼后,其阴栓与阴道涂片精子检出率及服药雄鼠的血清Ｔ、ＬＨ、ＦＳＨ测定值、和对照组相比均无统计学差异,表明受试雄鼠服用ＴＨ５后,可能并不干扰其性欲与血清性激素水平。     

Immunological changes during specific immunotherapy of grass pollen allergy: reduced lymphoproliferative responses to allergen and shift from TH2 to TH1 in T-cell clones specific for Phi p 1, a major grass pollen allergen

Background and Objective The mechanisms operative in specific immunotherapy (SIT) of Type I allergy are not completely understood. In the present study we evaluated immunological changes during SIT in pollinosis. Method Eight patients suffering from pollinosis (monosensitized to grass pollen) were treated with conventional SIT. All subjects had IgE specific for Phi p 1. a major allergen of timothy grass. In vitro changes in the immunological reactivity to grass pollen extract and to recombinant Phi p 1 were evaluated. Subjects were examined at three occasions: before, after 3 months and after I year of SIT. Results Serological analysis revealed a marked increase of grass pollen- and Phi p 1-specific IgG, titres of specific IgE did not change significantly. Lymphoproliferative responses to grass pollen extract and rPhl p 1 were reduced already after 3 months of treatment. Accordingly, the cloning efficiency for Ph1 p 1-specific T-cell clones (TCC) dropped markedly in all patients. The majority of allergen-specific TCC raised before SIT revealed a TH₂-like pattern of cytokine production. TCC established after SIT revealed TH₁ characteristics. This shift was due to a decrease in IL-4 rather than an increase in IFN-production by T cells. Investigations of the epitopes recognized by T cells before and after SIT did not reveal the outgrowth of new (ldquo;protecting”) specificities. We could not observe induction of allergen-speeific CD8⁺ lymphocytes (supressor cells). Conclusion Our data indicate that — on the level of TH lymphocytes — SIT induces tolerance to the allergen and a modulation of the cytokine pattern produced in response to allergen stimulation.     

强直性脊柱炎TH亚群激活及T细胞活化状态研究

目的 :研究强直性脊柱炎 (AS)患者TH1 TH2细胞激活状态及T细胞活化状况 ,探讨其发病机理。方法 :运用流式细胞仪 (CBA)法检测 35例AS患者TH1(INF γ、TNF α、IL 2 )、TH2 (IL 10、IL 5、IL 4 )细胞因子水平以及外周血淋巴细胞CD3 、CD4 、CD8 T细胞、B细胞 (CD19 )、NK细胞 (CD16 5 6 )和CD3 HLA DR 、CD4 HLA DR 、CD8 HLA DR T细胞百分率 ,并与健康对照组比较。结果 :AS患者血浆TNF α水平、IL 2水平均显著低于健康对照组 (P <0 0 1,P <0 0 5 ) ,IL 10水平显著高于健康对照组 (P <0 0 5 )。CD3 、CD3 CD8 T细胞百分率显著低于健康对照。CD8 HLA DR T细胞百分率均显著低于健康对照 (P <0 0 5 ) ,CD4 HLA DR T显著高于健康对照 (P <0 0 5 )。结论 :AS患者血浆低水平的TNF α、IL 2和高水平的IL 10提示其体内存在着TH1 TH2平衡的偏移 ;TH1激活程度低下 ,而TH2激活程度增强 ,细胞因子水平的改变尤以TH1细胞因子TNF α改变特别明显。AS患者在多个层面存在细胞免疫功能紊乱     

原代培养大鼠脊髓神经元损伤前后Fos、TH及PNMT表达变化的研究

为了探讨多巴胺及肾上腺素在神经元损伤和修复过程中的作用,我们通过锐器切割原代培养的纯化大鼠胚胎脊髓神经元,模拟脊髓全横断后脊髓神经元损伤状态,观察了损伤前后Fos蛋白与儿茶酚胺类神经递质合成酶的表达变化。分离、培养、纯化孕14d的Wistar大鼠脊髓神经元,10d后在直视下进行脊髓神经元的锐器切割损伤。用免疫组化及Western blot技术研究损伤前后神经元内Fos蛋白与酪氨酸羟化酶(TH)、苯乙醇胺氮位甲基移位酶(PNMT)的共定位情况及表达水平变化。结果发现:脊髓神经元损伤10min后Fos蛋白开始表达,2h后Fos阳性达到最高并同时表达TH及PNMT;Western blot结果也证实损伤后Fos、TH和PNMT表达均较损伤前上调。上述结果提示,神经元经损伤后激活的即刻早期基因c-fos,可作为信号分子活化受损神经元内儿茶酚胺类神经递质(TH和PNMT),多个分子共同作用可能参与了神经元的损伤和修复过程,其生物学意义有待进一步探讨。