脑梗塞与红细胞胆固醇,血液超氧化物歧化酶,过氧化脂质水平…

检测１０９例脑梗塞患者和１００例对照者红细胞胆固醇（Ｅ－Ｃｈ）含量、务 和红细胞超氧化物歧化酶（Ｐ－ＳＯＤ、Ｅ－ＳＯＤ）活性及血浆和红细胞过氧化脂质（Ｐ－ＰＯ、Ｅ－ＬＰＯ）含量的结果表明，患者组Ｅ－Ｃｈ、Ｐ－ＬＰＯ、Ｅ－ＬＰＯ平均含量皆显著高于对照组（Ｐ〈０．０５－０．００１），Ｐ－ＳＯＤ、Ｅ－ＳＯＤ平均活性皆显著低于对照组（Ｐ〈０．００１）；患者病情随Ｅ－Ｃｈ含量升高而加重，呈直线相关；患者Ｅ－     

多发性脑梗塞痴呆患者部分抗氧化指标检测及临床意义

检测配对设计的５４例多发性脑梗塞痴呆患者的５４例健康老年人血浆维生素Ｃ、血浆维生素Ｅ含量及血浆和红细胞超氧化物歧化酶活性、血浆和红细胞过氧化脂质、Ｅ－ＳＯＤ值均显著降低（Ｐ＜０．００１），平均Ｐ－ＬＰＯ和Ｅ－ＬＰＯ含量均显著升高；５４例患者的Ｐ－ＶＣ、Ｐ－ＶＥ含量均与Ｐ－ＳＯＤ、Ｅ－ＳＯＤ值呈直线正相关，均与Ｐ－ＬＰＯ、Ｅ－ＬＰＯ含量呈直线负相关；患者病程与Ｐ０ＶＣ、Ｐ－ＶＥ、Ｅ－ＳＯＤ值呈直线负     

脑中风与红细胞胆固醇,过氧化脂质和超氧化物歧化酶水平关系的研究

检测１０９例脑梗塞、１０５例脑出血患者和１００例健康对照者红细胞胆固醇（Ｅ－Ｃｈ）、血浆和红细胞过氧化脂质（Ｐ－ＬＰＯ、Ｅ－ＬＰＯ）含量及超氧休物歧化酶（Ｐ－ＳＯＤ、Ｅ－ＳＯＤ）活性的结果表明，与对照组比较，脑梗塞组Ｅ－Ｃｈ显著升高，脑出血组Ｅ－Ｃｈ显著降低；２患者组Ｐ－ＬＰＯ、Ｅ－ＬＰＯ显著升高，Ｐ－ＳＯＤ、Ｅ－ＳＯＤ显著降低；Ｅ－Ｃｈ与脑梗塞患者病情及Ｐ－ＬＰＯ、Ｅ－ＬＰＯ、Ｐ－ＳＯＤ、Ｅ－Ｓ     

吸烟者血浆VitC、VitE、β－CAR、LPO和E－SOD值的检测

检测３８４例健康老年吸烟者和１７６例健康老年非吸烟者血浆维生素Ｃ（Ｐ－ＶｉｔＣ）、维生素Ｅ（Ｐ－ＶｉｔＥ）、β－胡萝卜素（Ｐ－β－ＣＡＲ）、过氧化脂质（Ｐ－ＬＰＯ）含量和红细胞超氧化物歧化酶（Ｅ－ＳＯＤ）活性的结果表明，与非吸烟组比较，吸烟组的Ｐ－ＶｉｔＣ、Ｐ－ＶｉｔＥ、Ｐ－β－ＣＡＲ和Ｅ－ＳＯＤ平均值显著降低（Ｐ＜０．００１），而Ｐ－ＬＰＯ平均值显著升高（Ｐ＜０．００１）；８２例６１岁男性吸烟者Ｐ－ＶｉｔＣ、Ｐ－ＶｉｔＥ、Ｐ－β－ＣＡＲ和Ｅ－ＳＯＤ均随吸烟史及吸烟量的增加而降低，Ｐ－ＬＰＯ随吸烟史及吸烟量的增加而升高，并均呈一定程度的直线相关（Ｐ＜０．０００１）。提示老年吸烟者体内的氧化和抗氧化平衡严重失调，氧自由基反应和脂质过氧化反应明显加剧。     

一氧化氮等自由基与脑出血关系的研究

目的探讨一氧化氮等自由基与脑出血的关系。方法检测１３３例脑出血患者和１００例健康对照者血浆一氧化氮（Ｐ－ＮＯ）、维生素Ｃ（Ｐ－ＶＣ）、维生素Ｅ（Ｐ－ＶＥ）、β－胡萝卜素（Ｐ－β－ＣＡＲ）和过氧化脂质（Ｐ－ＬＰＯ）含量及红细胞超氧化物歧化酶（Ｅ－ＳＯＤ）、过氧化氢酶（Ｅ－ＣＡＴ）、谷胱甘肽过氧化物酶（Ｅ－ＧＳＨ－Ｐｘ）活性和过氧化脂质（Ｅ－ＬＰＯ）含量。结果与对照组比较,患者组Ｐ－ＮＯ、Ｐ－ＬＰＯ、Ｅ－ＬＰＯ均值显著升高（Ｐ＜０．００１）,Ｐ－ＶＣ、Ｐ－ＶＥ、Ｐ－β－ＣＡＲ、Ｅ－ＳＯＤ、Ｅ－ＣＡＴ、Ｅ－ＧＳＨ－Ｐｘ均值显著降低（Ｐ＜０．００１）;逐步回归发现,患者病情（ＮＤＳ）与Ｐ－ＮＯ、Ｐ－ＶＣ、Ｅ－ＬＰＯ值相关最为密切,颅内血肿量与Ｐ－ＮＯ、Ｐ－ＶＥ、Ｅ－ＬＰＯ值相关最为密切。结论脑出血患者体内自由基反应病理性加剧,氧化抗氧化平衡严重失     

NO和脂质过氧化与老年矽肺关系的研究

目的　探讨ＮＯ和脂质过氧化与老年矽肺的关系。方法　检测８５ 例老年矽肺患者和８０ 例健康老年人的血浆ＮＯ（Ｐ－ＮＯ）、过氧化脂质（Ｐ－ＬＰＯ）及红细胞超氧化物歧化酶（Ｅ－ＳＯＤ）、过氧化氢酶（Ｅ－ＣＡＴ）、谷胱甘肽过氧化物酶（Ｅ－ＧＳＨ－Ｐｘ）、过氧化脂质（Ｅ－ＬＰＯ）值。结果　与对照组比较,患者组Ｅ－ＳＯＤ、Ｅ－ＣＡＴ、Ｅ－ＧＳＨ－Ｐｘ 均值显著降低,Ｐ－ＮＯ、Ｐ－ＬＰＯ、Ｅ－ＬＰＯ 均值显著升高（Ｐ＜ ０．００１）;直线回归和相关分析表明上述各检测值与患者病程均有相关;逐步回归表明患者病情、肺功能状态与Ｐ－ＮＯ、Ｅ－ＳＯＤ、Ｅ－ＬＰＯ 值相关最密切。结论　矽肺患者体内ＮＯ 代谢异常,氧化抗氧化平衡严重失调,氧化和脂质过氧化反应病理性加剧。     

银杏叶抗氧化和抗脂质过氧化损伤效应的探讨

目的探讨银杏叶抗氧化和抗脂质过氧化损伤的效应。方法检测７８例心绞痛患者经银杏叶制剂天保宁治疗前后的血浆维生素Ｃ（Ｐ－ＶＣ）、维生素Ｅ（Ｐ－ＶＥ）、β－胡萝卜素（Ｐ－β－ＣＡＲ）、过氧化脂质（Ｐ－ＬＰＯ）和红细胞超氧化物歧化酶（Ｅ－ＳＯＤ）、过氧化氢酶（Ｅ－ＣＡＴ）、谷胱甘肽过氧化物酶（Ｅ－ＧＳＨ－ＰＸ）、过氧化脂质（Ｅ－ＬＰＯ）值，在Ｃｏｍｐａｑ５８６微机上用ＳＰＳＳ／ＰＣ＋统计软件包分析处理。结果与治疗前比较，治疗后的Ｐ－ＶＣ、Ｐ－ＶＥ、Ｐ－β－ＣＡＲ、Ｅ－ＳＯＤ、Ｅ－ＣＡＴ、Ｅ－ＧＳＨ－ＰＸ平均值皆显著升高（Ｐ＜０．０１），Ｐ－ＬＰＯ、Ｅ－ＬＰＯ平均值皆显著降低（Ｐ＜０．０１）；且均与对照组无显著差异（Ｐ＞０．０５）。结论心绞痛患者体内病理性氧化反应和脂质过氧化反应在治疗后明显减缓，银杏叶制剂具有较强的抗氧化损伤和抗脂质过氧化损伤的作用     

宫颈癌与妇科良性疾病患者血中抗氧化指标的检测及其临床意义

检测了２７例宫颈癌、３０例宫颈炎和３０例子宫肌瘤患者的血清ＳＯＤ及其同功酶、全血ＧＳＨ－Ｐｘ、ＣＡＴ等的活性水平以及血清ＬＰＯ含量。结果表明，与健康对照组相比，宫颈癌患者血清Ｃｕ，Ｚｎ－ＳＯＤ、总－ＳＯＤ（Ｔ－ＳＯＤ）活性明显降低（Ｐ＜０．０１），ＬＰＯ含量明显增高（Ｐ＜０．０５）；宫颈炎患者血清Ｍｎ－ＳＯＤ、Ｔ－ＳＯＤ活性明显降低（Ｐ＜０．０１），全血ＧＳＨ－Ｐｘ活性与血清ＬＰＯ含量明显增高（Ｐ＜０．０１）；子宫肌瘤患者血清Ｍｎ－ＳＯＤ、Ｃｕ、Ｚｎ－ＳＯＤ、Ｔ－ＳＯＤ与全血ＧＳＨ－Ｐｘ活性均明显降低（Ｐ＜０．０１），血清ＬＰＯ含量明显增高（Ｐ＜０．０１）。可见，血清Ｔ－ＳＯＤ活性降低和ＬＰＯ含量升高是宫颈癌、宫颈炎与子宫肌瘤患者自由基代谢紊乱的共同特点，提示体内病理性氧自由基反应和脂质过氧化反应明显增强是上述患者共同的病理基础。     

前列腺素E1对肺心病心衰疗效的观察

探讨前列腺素Ｅ１静脉滴注对肺心病心衰的疗效。方法对３０例肺心病心衰患者，应用前列腺素Ｅ１（ＰＧＥ１）２００μｇ／ｄ静脉滴注，疗程５天。于治疗前后同步检测内源性洋地黄因子（ＥＤＦ）、过氧化脂质（ＬＰＯ）、超氧化物歧化酶（ＳＯＤ）及动脉血氧分压（ＰａＯ２）、动脉血二氧化碳分压（ＰａＣＯ２）和观察症状体征变化，并与对照组（不用ＰＧＥ１）的３０例肺心病心衰患者及２０例健康者进行比较。结果两组肺心病心衰患者的ＥＤＦ、ＬＰＯ显著高于健康组（Ｐ＜０．００１），ＳＯＤ含量则显著降低（Ｐ＜０．００１）。治疗组病例治疗后ＥＤＦ、ＬＰＯ较治疗前显著降低（Ｐ＜０．００１），ＳＯＤ含量则显著升高（Ｐ＜０．００１），症状体征及血气参数显著改善（Ｐ＜０．０１～０．００１）。对照组（不用ＰＧＥ１）病例的ＥＤＦ、ＬＰＯ、ＳＯＤ、血气参数及症状体征，于治疗前、后无显著变化（Ｐ＞０．０５）。结论ＰＧＥ１对肺心病心衰患者确有较好的疗效。     

老年人脑出血与一氧化氮等自由基关系的探讨

１９９４年３月至１９９６年９月检测了９８例老年脑出血急性期患者血浆一氧化氮（ＰＮＯ）、维生素Ｃ（ＰＶＣ）、维生素Ｅ（ＰＶＥ）、血浆和红细胞过氧化脂质（ＰＬＰＯ、ＥＬＰＯ）含量及血浆和红细胞超氧化物歧化酶（ＰＳＯＤ、ＥＳＯＤ）活性,并与１...     

老年心绞痛患者银杏叶黄酮治疗前后的自由基变化

以邻苯三酚自氧化抑制法和硫代巴比妥酸反应产物比色分析法检测经用“天保宁”(银杏叶制剂)治疗50例心绞痛患者前后红细胞超氧化物歧化酶(E—SOD)活性及血浆过氧化脂质(P—LPO)和红细胞过氧化脂质(E—LPO)含量的结果表明,与治疗前相比,治疗后的E-SOD平均活性显著升高(P<0.01),P—LPO和E—LPO平均含量均显著降低(P<0.01)。提示老年心绞痛患者体内的病理性氧自由基反应和脂质过氧化反应在治疗后明显减缓,银杏叶黄酮具有较强的抗氧自由基损伤和抗脂质过氧化损伤作用。     

A study on relationship of nitric oxide, oxidation, peroxidation, lipoperoxidation with chronic chole-cystitis

AIM To study relationship of injury induced by nitric oxide, oxidation, peroxidation, lipoperoxidation with chronic cholecystitis. METHODS The values of plasma nitric oxide (PNO), plasma vitamin C (P-VC), plasma vitamin E ( P-VE ), plasma β-carotene ( P-β-CAR ), plasma lipoperoxides (P-LPO), erythrocyte superoxide dismutase (E-SOD), erythrocyte catalase (ECAT), erythrocyte glutathione peroxidase (E-GSHPx) activities and erythrocyte lipoperoxides (ELPO) level in 77 patients with chronic cholecystitis and 80 healthy control subjects were determined, differences of the above average values between the patient group and the control group and differences of the average values between preoperative and postoperative patients were analyzed and compared, linear regression and correlation of the disease course with the above determination values as well as the stepwise regression and correlation of the course with the values were analyzed. RESULTS Compared with the control group, the average values of P-NO, P-LPO, E-LPO were significantly increased ( P < 0.01 ), and of P-VC, P-VE, P-β-CAR, E-SOD, E-CAT and E-GSH-Px decreased ( P < O. 01 ) in the patient group. The analysis of the linear regression and correlation showed that with prolonging of the course, the values of P-NO, P-LPO and E-LPO in the patients were gradually ascended and the values of P-VC, P-VE, P-β-CAR, E-SOD, E-CAT and E-GSH-Px descended (P < 0. 01 ). The analysis of the stepwise regression and correlation indicated that the correlation of the course with P-NO, P-VE and P-β-CAR values was the closest. Compared with the preoperative patients, the average values of PNO, P-LPO and E-LPO were significantly decreased (P < 0.01 ) and the average values of PVC, E-SOD, E-CAT and E-GSH-Px in postoperative patients increased ( P < 0.01) in postoperative patients. But there was no significant difference in the average values of P-VE, P-W-CAR preoperative and postoperative patients. CONCLUSION Chronic cholecystitis could induce the increase of nitric oxide, oxidation, peroxidation and lipoperoxidation.     

Therapeutic efficacy of high-dose vitamin C on acute pancreatitis and its potential mechanisms

AIM: To observe the therapeutic efficacy of high-dose Vitamin C 0/it. C) on acute pancreaUtis (AP), and to explore its potential mechanisms. METHODS: Eghty-four AP patients were divided into treatment group and control group, 40 healthy subjects were taken as a normal group, In the treatment group, Vit. C (10 g/day) was given intravenously for 5 days, whereas in the control group, Vit. C (1 g/day) was given intravenously for 5 days. Symptoms, physical signs, duration of hospitalization, complications and mortality rate were monitored. Meanwhile, serum amylase, urine amylase and leukocyte counts were also determined. The concentration of plasma vitamin C (P-VC), plasma lipid peroxide (P-LPO), plasma vitamin E (P-VE), plasma β-carotene (P-β-CAR), whole blood glutathione (WB-GSH) and the activity of erythrocyte surperoxide dimutase (E-SOD) and erythrocyte catalase (E-CAT) as well as T lymphocyte phenotype were measured by spectrophotometry in the normal group and before and after treatment with Vit. C in the treatment and the control group. RESULTS: Compared with the normal group, the average values of P-VC, P-VE, P-β-CAR, WB-GSH and the activity of E-SOD and E-CAT in AP patients were significantly decreased and the average value of P-LPO was significantly increased, especially in severe acute pancreatitis (SAP) patients (P&lt;0.05. P-VC, P=0.045; P-VE, P=0.038; P=0.041;P-β-CAR, P=0.046; WB-GSH, P=0.039; E-SOD, P=0.019;E-CAT, P=0.020; P-LPO, P=0.038). Compared with the normal group, CD3 and CD4 positive cells in AP patients were significantly decreased. The ratio of CD4/CD8 and CD4 positive cells were decreased, especially in SAP patients (P&lt;0.05. CD4/CDs, P=0.041; CD4, P =0.019). Fever and vomiting disappeared, and leukocyte counts and amylase in urine and blood become normal quicker in the treatment group than in the control group. Moreover, patients in treatment group also had a higher cure rate, a lower complication rate and a shorter in-ward days compared with those in he control group. After treatment, the average value of P-VC was significantly higher and the values of SIL-2R, TNF-α, IL-6 and IL-8 were significantly lower in the treatment group than in the control group (P&lt;0.05 P-VC, P=0.045; SIL-2R, P=0.012; TNF-α, P=0.030; IL-6, P=0.015,and IL-8, P=0.043). In addition, the ratio of CDJCD8 and CD4 positive cells in the patients of treatment group were significantly higher than that of the control group after treatment (P&lt;0.05. CD4/CDs, P =0.039; CD4, P=0.024). CONCLUSION: High-dose vitamin C has therapeutic efficacy on acute pancreatitis. The potential mechanisms include promotion of anti-oxidizing ability of AP patients, blocking of lipid peroxidation in the plasma and improvement of cellular immune function.     

Measurement of plasma malondialdehyde contents and erythrocyte superoxide dismutase activities in patients with chronic cor pulmonale]

We measured the contents of plasma MDA and erythrocyte SOD activities in patients with chronic cor pulmonale. The contents of malondialdehyde (MDA) increased; the superoxide dismutase (SOD) activities suppressed significantly and reoxygenation caused increases in the SOD activities in patients with acute attacks. But the changes of the contents of MDA and SOD activities did not appear in patients with stationary phase. These results indicate that oxygen free radicals may contribute to the physiopathological process of chronic cor pulmonale.     

人红细胞胆固醇含量的增龄变化

用胆固醇酶联试剂显色法检测１８９例健康成人红细胞胆固醇和血浆胆固醇含量，结果表明，健康成人的Ｅ－Ｃｈ含量随增龄而升高，并呈直线相关；Ｐ－Ｃｈ含量也随增龄而升高，并呈直线相关；Ｅ－Ｃｈ含量呈直线正样关。提示Ｅ－Ｃｈ含量检测可作为反映机体衰老程度的一项客观指标。     

Levels of plasma malondialdehyde and erythrocyte antioxidant enzyme activities in patients with chronic hepatitis B

BACKGROUND/AIMS: Hepatitis B virus infection, extensively seen throughout the world, can become highly chronic. Pathogenesis of chronic hepatitis is not yet known fully. It is shown that oxidative stress may play a role in pathogenesis and may regulate collagen synthesis and thus may contribute to the process of liver damage. This study is aimed at investigating the existence of oxidative stress in chronic hepatitis B cases and its relation with alanine aminotransferase and aspartate aminotransferase which are the serum indicators of liver damage; along with interaction of erythrocyte antioxidation enzyme activities in the same cases. METHODOLOGY: Eighty patients with chronic hepatitis B under follow-up, and 40 healthy volunteers were included in this study. In the control and patients groups, together with serological markers for viral etiology, alanine aminotransferase and aspartate aminotransferase levels; plasma malondialdehyde level; erythrocyte superoxide dismutase, glutathione peroxidase and catalase activities were analyzed. RESULTS: Malondialdehyde levels of chronic hepatitis B cases were statistically high compared to control group (p < 0.05). There was correlation between serum malondialdehyde levels and serum alanine aminotransferase, aspartate aminotransferase levels in the patient group (r = 0.324, p < 0.01, r = 0.273, p < 0.05). Average superoxide dismutase and catalase activities were found to be significantly low compared to control group (p < 0.001); average glutathione peroxidase activity were significantly high when considered statistically (p < 0.001). No correlation between serum alanine aminotransferase and aspartate aminotransferase levels and glutathione peroxidase, catalase and superoxide dismutase activities was found in the patients group (p > 0.05). CONCLUSIONS: In the study we showed that there is correlation between serum malondialdehyde level and alanine aminotransferase and aspartate aminotransferase levels of chronic hepatitis B patients. According to the results of our study, it might be thought that serum malondialdehyde level might be a marker of hepatocellular damage in chronic hepatitis B cases. We suggest that antioxidant treatment for chronic hepatitis B patients should be examined in future studies.     

糖尿病病人红细胞超氧化物岐化酶和血浆过氧化脂质的变化

本文测定了47例糖尿病病人及47例正常人红细胞超氧化物岐化酶(SOD)及血浆过氧化脂质(Lpo)水平。结果发现,糖尿病病人红细胞SOD明显低于正常人(P<0.001),Lpo水平明显高于正常人(P<0.001)。糖尿病病人中伴有血管并发症者与无血管并发症者相比,红细胞SOD活性下降幅度更大(P<0.05),血浆Lpo增高更明显(P<0.02)。血浆Lpo与HDL-C、红细胞SOD呈负相关,与甘油三酯、空腹血糖呈正相关;红细胞SOD与血糖及血脂无明显相关关系。